LOCUS       sequence-310012-       24935 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Recipient plant transformation vector containing selection markers 
            and a Cas9 expression cassette, but lacking guide RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 24935)
  AUTHORS   Stuttmann J, Barthel K, Martin P, Ordon J, Erickson JL, Herr R, 
            Ferik F, Kretschmer C, Berner T, Keilwagen J, Marillonnet S, Bonas U
  TITLE     Highly efficient multiplex editing: One-shot generation of 8x 
            Nicotiana benthamiana and 12x Arabidopsis mutants.
  JOURNAL   Plant J. 2021 Feb 12. doi: 10.1111/tpj.15197.
  PUBMED    33577114
REFERENCE   2  (bases 1 to 24935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2021 Feb 12. doi: 10.1111/tpj.15197."
COMMENT     Sequence Label: pDGE673
FEATURES             Location/Qualifiers
     source          1..24935
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1062..1691
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             2120..3193
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      3259..3453
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3689..3711)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3797..3937
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3852..3871
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3952..3969)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4123..4711)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4203..4222)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4957..5748)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASCADQLEEFVHYVKGEITKVVGK"
     misc_feature    6269..6293
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     terminator      complement(6382..6634)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     promoter        complement(7234..7416)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(7270..7448)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     CDS             complement(8085..8813)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="VSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMR
                     IKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGV
                     LTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTDM
                     ALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVA
                     RYCDLPSKLGHKLNGMDELYK"
     CDS             complement(8826..9557)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     terminator      complement(11186..11893)
                     /label=OCS terminator
                     /note="octopine synthase terminator"
     CDS             complement(15142..15162)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(join(20892..21602,21603..21605))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(20934..20955)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     21283..21302
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     21542..21563
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             complement(21618..21644)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     promoter        complement(21741..22086)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(21778..21798)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     promoter        23110..23212
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             23213..23896
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGRKRR
                     VDPAY"
     primer_bind     complement(23279..23298)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             24216..24521
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     24400..24419
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     misc_feature    24642..24666
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ttcctggcgt tttcttgtcg cgtgttttag tcgcataaag tagaatactt gcgactagaa
       61 ccggagacat tacgccatga acaagagcgc cgccgctggc ctgctgggct atgcccgcgt
      121 cagcaccgac gaccaggact tgaccaacca acgggccgaa ctgcacgcgg ccggctgcac
      181 caagctgttt tccgagaaga tcaccggcac caggcgcgac cgcccggagc tggccaggat
      241 gcttgaccac ctacgccctg gcgacgttgt gacagtgacc aggctagacc gcctggcccg
      301 cagcacccgc gacctactgg acattgccga gagcatccag gaggccggcg cgggcctgcg
      361 tagcctggca gagccgtggg ccgacaccac cacgccggcc ggccgcatgg tgttgaccgt
      421 gttcgccggc attgccgagt tcgagcgttc cctaatcatc gaccgcaccc ggagcgggcg
      481 cgaggccgcc aaggcccgag gcgtgaagtt tggcccccgc cctaccctca ccccggcaca
      541 gatcgcgcac gcccgcgagc tgatcgacca ggaaggccgc accgtgaaag aggcggctgc
      601 actgcttggc gtgcatcgct cgaccctgta ccgcgcactt gagcgcagcg aggaagtgac
      661 gcccaccgag gccaggcggc gcggtgcctt ccgtgaggac gcattgaccg aggccgacgc
      721 cctggcggcc gccgagaatg aacgccaaga ggaacaagca tgaaaccgca ccaggacggc
      781 caggacgaac cgtttttcat taccgaagag atcgaggcgg agatgatcgc ggccgggtac
      841 gtgttcgagc cgcccgcgca cgtctcaacc gtgcggctgc atgaaatcct ggccggtttg
      901 tctgatgcca agctggcggc ctggccggcc agcttggccg ctgaagaaac cgagcgccgc
      961 cgtctaaaaa ggtgatgtgt atttgagtaa aacagcttgc gtcatgcggt cgctgcgtat
     1021 atgatgcgat gagtaaataa acaaatacgc aaggggaacg catgaaggtt atcgctgtac
     1081 ttaaccagaa aggcgggtca ggcaagacga ccatcgcaac ccatctagcc cgcgccctgc
     1141 aactcgccgg ggccgatgtt ctgttagtcg attccgatcc ccagggcagt gcccgcgatt
     1201 gggcggccgt gcgggaagat caaccgctaa ccgttgtcgg catcgaccgc ccgacgattg
     1261 accgcgacgt gaaggccatc ggccggcgcg acttcgtagt gatcgacgga gcgccccagg
     1321 cggcggactt ggctgtgtcc gcgatcaagg cagccgactt cgtgctgatt ccggtgcagc
     1381 caagccctta cgacatatgg gccaccgccg acctggtgga gctggttaag cagcgcattg
     1441 aggtcacgga tggaaggcta caagcggcct ttgtagtgtc gcgggcgatc aaaggcacgc
     1501 gcatcggcgg tgaggttgcc gaggcgctgg ccgggtacga gctgcccatt cttgagtccc
     1561 gtatcacgca gcgcgtgagc tacccaggca ctgccgccgc cggcacaacc gttcttgaat
     1621 cagaacccga gggcgacgct gcccgcgagg tccaggcgct ggccgctgaa attaaatcaa
     1681 aactcatttg agttaatgag gtaaagagaa aatgagcaaa agcacaaaca cgctaagtgc
     1741 cggccgtccg agcgcacgca gcagcaaggc tgcaacgttg gccagcctgg cagacacgcc
     1801 agccatgaag cgggtcaact ttcagttgcc ggcggaggat cacaccaagc tgaagatgta
     1861 cgcggtacgc caaggcaaga ccattaccga gctgctatct gaatacatcg cgcagctacc
     1921 agagtaaatg agcaaatgaa taaatgagta gatgaatttt agcggctaaa ggaggcggca
     1981 tggaaaatca agaacaacca ggcaccgacg ccgtggaatg ccccatgtgt ggaggaacgg
     2041 gcggttggcc aggcgtaagc ggctgggttg tctgccggcc ctgcaatggc actggaaccc
     2101 ccaagcccga ggaatcggcg tgagcggtcg caaaccatcc ggcccggtac aaatcggcgc
     2161 ggcgctgggt gatgacctgg tggagaagtt gaaggccgcg caggccgccc agcggcaacg
     2221 catcgaggca gaagcacgcc ccggtgaatc gtggcaagcg gccgctgatc gaatccgcaa
     2281 agaatcccgg caaccgccgg cagccggtgc gccgtcgatt aggaagccgc ccaagggcga
     2341 cgagcaacca gattttttcg ttccgatgct ctatgacgtg ggcacccgcg atagtcgcag
     2401 catcatggac gtggccgttt tccgtctgtc gaagcgtgac cgacgagctg gcgaggtgat
     2461 ccgctacgag cttccagacg ggcacgtaga ggtttccgca gggccggccg gcatggccag
     2521 tgtgtgggat tacgacctgg tactgatggc ggtttcccat ctaaccgaat ccatgaaccg
     2581 ataccgggaa gggaagggag acaagcccgg ccgcgtgttc cgtccacacg ttgcggacgt
     2641 actcaagttc tgccggcgag ccgatggcgg aaagcagaaa gacgacctgg tagaaacctg
     2701 cattcggtta aacaccacgc acgttgccat gcagcgtacg aagaaggcca agaacggccg
     2761 cctggtgacg gtatccgagg gtgaagcctt gattagccgc tacaagatcg taaagagcga
     2821 aaccgggcgg ccggagtaca tcgagatcga gctagctgat tggatgtacc gcgagatcac
     2881 agaaggcaag aacccggacg tgctgacggt tcaccccgat tactttttga tcgatcccgg
     2941 catcggccgt tttctctacc gcctggcacg ccgcgccgca ggcaaggcag aagccagatg
     3001 gttgttcaag acgatctacg aacgcagtgg cagcgccgga gagttcaaga agttctgttt
     3061 caccgtgcgc aagctgatcg ggtcaaatga cctgccggag tacgatttga aggaggaggc
     3121 ggggcaggct ggcccgatcc tagtcatgcg ctaccgcaac ctgatcgagg gcgaagcatc
     3181 cgccggttcc taatgtacgg agcagatgct agggcaaatt gccctagcag gggaaaaagg
     3241 tcgaaaaggt gtctttcctg tggatagcac gtacattggg aacccaaagc cgtacattgg
     3301 gaaccggaac ccgtacattg ggaacccaaa gccgtacatt gggaaccggt cacacatgta
     3361 agtgactgat ataaaagaga aaaaaggcga tttttccgcc taaaactctt taaaacttat
     3421 taaaactctt aaaacccgcc tggcctgtgc ataactgtct ggccagcgca cagccgaaga
     3481 gctgcaaaaa gcgcctaccc ttcggtcgct gcgctcccta cgccccgccg cttcgcgtcg
     3541 gcctatcgcg gccgctggcc gctcaaaaat ggctggccta cggccaggca atctaccagg
     3601 gcgcggacaa gccgcgccgt cgccactcga ccgccggcgc ccacatcaag gcaccctgcc
     3661 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     3721 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     3781 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     3841 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     3901 accgcacaga tgcgtaagga gaaaataccg cataaggcgc tcttccgctt cctcgctcac
     3961 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4021 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4081 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4141 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4201 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4261 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4321 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4381 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4441 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4501 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4561 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4621 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4681 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     4741 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgcatg atatatctcc
     4801 caatttgtgt agggcttatt atgcacgctt aaaaataata aaagcagact tgacctgata
     4861 gtttggctgt gagcaattat gtgcttagtg catctaacgc ttgagttaag ccgcgccgcg
     4921 aagcggcgtc ggcttgaacg aatttctagc tagacattat ttgccgacta ccttggtgat
     4981 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcacgagg ccaagcgatc
     5041 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     5101 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     5161 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     5221 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     5281 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     5341 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     5401 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     5461 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     5521 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     5581 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     5641 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     5701 tacctctgat agttgagtcg atacttcggc gatcaccgct tcccccatga tgtttaactt
     5761 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     5821 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     5881 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     5941 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     6001 ggcttatgtc cactgggttc gtgcccgaat tgatcacagg cagcaacgct ctgtcatcgt
     6061 tacaatcagc atgctaccct ccgcgagatc atccgtgttt aaaacccggc agcttagttg
     6121 ccgttcttcc gaatagcatc ggtaacatga gcaaagtctg ccgccttaca acggctctcc
     6181 cgctgacgcc gtcccggact gatgggctgc ctgtatcgag tggtgatttt gtgccgagct
     6241 gccggtcggg gagctgttgg ctggctggtg gcaggatata ttgtggtgta aacaaattga
     6301 cgcttagaca acttaataac acattgcgga tccctcggtc acatgtgcat cctccacaag
     6361 cgctccaatt cagcaagcgt cgatctagta acatagatga caccgcgcgc gataatttat
     6421 cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
     6481 taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct
     6541 taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc
     6601 ttaagaaact ttattgccaa atgtttgaac gatctgcttg actctagatc cagagtcccg
     6661 ctcaaatctc agtcacagga agaacaggtc taggaggaac aggaagagag aaatcaagct
     6721 gccagaatcc cacatcatgc caatttccat gtttgaaacc agcagctcta agcattcccc
     6781 taggagcata accaagagcc tcatgcatcc tcacagaagg atcattagga agtccaatca
     6841 cagcaaccac agacttgaaa ccctgagcct ccaaagactt caaaagatga gtgtaaagag
     6901 tagatccaag accagttctt tgatgcctag gagacacgta aacagtagac tcagcagtcc
     6961 aatcgtaagc attcctagcc ttccaaggac cagcataagc aataccagcc acttcaccat
     7021 ccacttcagc aacaagccaa ggatacctct ctctaagcct cacaagatca tcagtccact
     7081 cttgtggttc ttgaggctca gtcctgaaat tcacagtaga agtctcaatg taatgattca
     7141 caatagtgca cacagcaggc atatcagcct cagtagccct tctaatatca gcaggccttc
     7201 tttcaggaga catgcgaaac gatccagatc cggtgcagat tatttggatt gagagtgaat
     7261 atgagactct aattggatac cgaggggaat ttatggaacg tcagtggagc attttgacaa
     7321 gaaatatttg ctagctgata gtgaccttag gcgacttttg aacgcgcaat aatggtttct
     7381 gacgtatgtg cttagctcat taaactccag aaacccgcgg ctgagtggct ccttcaacgt
     7441 tgcggttctg tcagttccaa acgtaaaacg gcttgtcccg cgtcatcggc gggggtcata
     7501 acgtgactcc cttaattctc cgctccgcat gcgaattctc tgagcggtac actgccagat
     7561 gaagccgaag gagtacttta atatactagg tctggcgata tgatcaattt gaaaatatag
     7621 agtcataact gtagaaatgg aatcagattc cattacaaaa agagataaat ctcaacttta
     7681 aattttttca ggaagaaaaa aatgtttctt ctaatcttcg tgttttactg gccttctcat
     7741 aaaatggttt gatataaaag aaaattaaat aagaaaaaga gaaaacaaaa ggctagccag
     7801 agagaggaga aaatatataa taatataact tagtttattc atctcataat ctctgcattt
     7861 tgcaaggaga tcgaaactga gcaaaacata cacaaacaga gaaataaaat atagggtaca
     7921 aacaatatca atagcttaga caacatgaag ctacaaaaga acttcgcctc ctgtggggct
     7981 tattatttac tctttttttt ttcttattct tcgtcttttc tcattgcctc tcgaaattgg
     8041 aaaaagcctt tcttttagct caacattctg cttttccaag ctcatttgta cagctcgtcc
     8101 atgccattaa gtttgtgccc cagtttgcta gggaggtcgc agtatctggc cacagccacc
     8161 tcgtgctgct cgacgtaggt ttctttgtcg gcctccttga ttctttccag tctgtggtcc
     8221 acatagtaga cgccgggcat cttgaggttc ttagcgggtt tcttggatct gtatgtggtc
     8281 ttgaagttgc agatcaggtg gcccccgccc acgagcttca gggccatgtc ggttctgcct
     8341 tccaggccgc cgtcagcggg gtacagcatc tcggtgttgg cctcccagcc gagtgttttc
     8401 ttctgcatca cagggccgtt ggatgggaag ttcacccctc tgatcttgac gttgtagatg
     8461 aggcagccgt cctggaggct ggtgtcctgg gtagcggtca gcacgccccc gtcctcgtat
     8521 gtggtgactc tctcccatgt gaagccctca gggaaggact gcttaaagaa gtcggggatg
     8581 ccctgggtgt ggttgatgaa ggttctgctg ccgtacatga agctggtagc caggatgtcg
     8641 aaggcgaagg ggagagggcc gccctcgacc accttgattc tcatggtctg ggtgccctcg
     8701 tagggcttgc cttcgccctc ggatgtgcac ttgaagtggt ggttgttcac ggtgccctcc
     8761 atgtacagct tcatgtgcat gttctcctta atcagctctt cgcccttaga cacggcagcc
     8821 gaagctttgt acagctcgtc catgccatta agtttgtgcc ccagtttgct agggaggtcg
     8881 cagtatctgg ccacagccac ctcgtgctgc tcgacgtagg tttctttgtc ggcctccttg
     8941 attctttcca gtctgtggtc cacatagtag acgccgggca tcttgaggtt cttagcgggt
     9001 ttcttggatc tgtatgtggt cttgaagttg cagatcaggt ggcccccgcc cacgagcttc
     9061 agggccatgt cggttctgcc ttccaggccg ccgtcagcgg ggtacagcat ctcggtgttg
     9121 gcctcccagc cgagtgtttt cttctgcatc acagggccgt tggatgggaa gttcacccct
     9181 ctgatcttga cgttgtagat gaggcagccg tcctggaggc tggtgtcctg ggtagcggtc
     9241 agcacgcccc cgtcctcgta tgtggtgact ctctcccatg tgaagccctc agggaaggac
     9301 tgcttaaaga agtcggggat gccctgggtg tggttgatga aggttctgct gccgtacatg
     9361 aagctggtag ccaggatgtc gaaggcgaag gggagagggc cgccctcgac caccttgatt
     9421 ctcatggtct gggtgccctc gtagggcttg ccttcgccct cggatgtgca cttgaagtgg
     9481 tggttgttca cggtgccctc catgtacagc ttcatgtgca tgttctcctt aatcagctct
     9541 tcgcccttag acaccatttt tgctatttgt gtatgttttc ttgttttggt ttgagtgaag
     9601 agtgtagtga agggttggtt tgtatttata ggtgagaaag gagatttgca tggcaatcac
     9661 gtgtaagaat gcatgcatgc atgagtgtgt gtggagagag agagatgctc ttacgttgtt
     9721 gtcgggatta tgtgttggaa aagagtgaca agtgacgaac aaaacaatac cactcatcat
     9781 agctccgctt atgcatgcta tgtgtgattt gtagctcttg ttctgacatg acactatgaa
     9841 caatttttta aaaagaaggt taaactttta tttaaataaa aaccctatgt taatttggtt
     9901 tcaatcgagt aagatgagaa gtcaatcttt tgatggtagc tagtgatgtt ggaaatatag
     9961 ttgtgccagc ttaatctgaa atttagaata taattttata agagtatcta tcaatatata
    10021 tattaacggg tcactcacga acaatgcatg taagaaattt ttatgaggga tatatatgtt
    10081 aagaaaatac cgaattatat gtgtacgtat acacacataa ttagaggtgt caaaatacat
    10141 caaaactcgt ggttaagggt ttaccaaaaa aattataact caaaccgtga agtaggagta
    10201 aaagaaatta ccaaaaccat gttttgacat aaaattgaaa acagagcgat gaagagaata
    10261 agaagatgat acgtacaaaa tcttacgagt tatgaatatc gagagtttta caccaacaaa
    10321 tcaaaaacca aaatcgagag ttcgtacgtc tcgactaaat caaagctagc aattcttagc
    10381 tatgtttttc atttcaaaga gttaccaawa wraaaaaaaa aaaaaaaaaa aaaragttac
    10441 cactatgaat tactcaaaga gtaaacaata tgaataatac aaatccgatc gatatcactg
    10501 atcaagaaac acattatatt ctaactttct aaccacggtt agcttgaacc aaactaaacc
    10561 ttctcaacac cagagcaatg gccttacata aattctcatc agaaaatggt cgaccagctg
    10621 ttaaccagaa ctaattgtat tcacagaaca acatcaacac gtttttttta taaagacatt
    10681 tgatcggtac gttttacact cttggccaaa atggctagca cttttttacc acaattgtaa
    10741 gcgactatcg atcaaaccga tcatgtaatg taaaatttcg tttttgaact tcgcttccaa
    10801 tggatcatct aatttaaaat taaaatttgg catgtctctt ccacttgatg ccgtgcaatt
    10861 tcttgagccc aaaaaataga tattaacacg catgcggtgg ggcggtggta actagactat
    10921 ggcgtaaatt cacacatgca taatgcttca ttctcatagc gaataaattt tcttcgtgtt
    10981 gggtcactaa ttggatctgt gctttggttt tgtgttttat ccatggtgtt gtgttccttg
    11041 attatatcat atggataaat gtttaatact tgatagttga tcagatgata tagaactagt
    11101 tgcaaatcta tcatcttgaa tgaaaacaca ccgtctgtta gactcgcaaa tttatcttct
    11161 ccccatggga attcgtaaag cggtcctgct gagcctcgac atgttgtcgc aaaattcgcc
    11221 ctggacccgc ccaacgattt gtcgtcactg tcaaggtttg acctgcactt catttggggc
    11281 ccacatacac caaaaaaatg ctgcataatt ctcggggcag caagtcggtt acccggccgc
    11341 cgtgctggac cgggttgaat ggtgcccgta actttcggta gagcggacgg ccaatactca
    11401 acttcaagga atctcaccca tgcgcgccgg cggggaaccg gagttccctt cagtgagcgt
    11461 tattagttcg ccgctcggtg tgtcgtagat actagcccct ggggcacttt tgaaatttga
    11521 ataagattta tgtaatcagt cttttaggtt tgaccggttc tgccgctttt tttaaaattg
    11581 gatttgtaat aataaaacgc aattgtttgt tattgtggcg ctctatcata gatgtcgcta
    11641 taaacctatt cagcacaata tattgttttc attttaatat tgtacatata agtagtaggg
    11701 tacaatcagt aaattgaacg gagaatatta ttcataaaaa tacgatagta acgggtgata
    11761 tattcattag aatgaaccga aaccggcggt aaggatctga gctacacatg ctcaggtttt
    11821 ttacaacgtg cacaacagaa ttgaaagcaa atatcatgcg atcataggct tctcgcatat
    11881 ctcattaaag caggacaagc ctacatttgt tctttccaaa ttttggcaat atcttgtgca
    11941 acattagtgg catctataga agcaccaaac agtcctattc ctgtaaatcc aactgcatac
    12001 agaccatcct cccccttcca accatttggg aatgggcttt ttggacatcc ctcccttgaa
    12061 aacaattcac tctcctgtat cagtaaaatt ctcattagac caaatttcga agggggctat
    12121 tatagaacga gagaaagttg ctcagatggt aaagagcccc ttattcccaa tcttaagatt
    12181 gtgagttcga gttatcaaaa gaacaaaaag gtatgaggaa agggtaaaag aaaaaagacg
    12241 gttatagtga attctaacta attaataact caaaggaaag aatccacagg ataatgagat
    12301 accaacttac atgaatgtaa caagaatcca taaataatta ctacattcat taagagttca
    12361 tttgactaaa tcatccttta tagttgaagg agcttttatc atacttagta ctatatataa
    12421 tttatgatta aacaccaatt taattaggca tagaaataaa acttgtgtat ttccttacca
    12481 ttaaccaaga agttacattg ctggtataac cagttgccaa gataacagag tcgatctcta
    12541 gaatttgtcc attaacaaat tctacttttc cttgagtaaa tttcttgatt gctggaacta
    12601 tgtttatttc ctcagggaaa ttacgaccct gtacctgaaa gattaacaaa actagatgat
    12661 atcattcatg tgtgaatata atatattcat gcgagcaaat gaaagataca agatcacttt
    12721 ctagtgttac ttgaaaaata atacttattg aatataaaac ttaccgagct tcgaactacc
    12781 atgaatggat ttgcattatg ttgggaaaga tcaagtgaga tatcgatccc ggaattgcca
    12841 cagccaaccg ccagcacatt ttctccagta tagtattccc cagtcttgta ctcacaagca
    12901 tgaataacct ggccaccaaa atcttgcaat ccttcgaatt cgggcactat catctcagca
    12961 ttctctcctg tggccacaat aagccactta cacatgtatt cagaggttga accattgatt
    13021 tcagaaactg ttttcacctt ccataaacca catgtctcat catatccagc taagtttact
    13081 gactcgttga gttgtggttt gatctcgaaa tgctttgcat aagatacgag gtagctgatg
    13141 aattggtttt tggttggata ctctggaaag tctggtggaa atggcaagcc aggcaattcg
    13201 cagtattgtc gtggcacgtt aagcctaagc cgatcgtagg tcttgtgttg ccacagagaa
    13261 gcaatgcagt ccgcgcgttc aatgattaca tacggaacac tgtattgctt aaggacagca
    13321 gcagtagcca ggcctgaagg gccagctcca acaataagag gaccatttac ttgtatgcat
    13381 ttagcagcac aagaggattt ttttggttca agtgcatcaa cagttagagg tgaacaagaa
    13441 ttaaagcaat tctgattcat cattaaatat atgtgcaact aggactactt gtgttttgat
    13501 agatttagcg ggtgacaaga acaagaggag aaaagagaaa ggggatgata acttgaagtt
    13561 gtgaggatgg ttaggtttat ataataaaat tggtcaggca aacgtgttca ttgtttaacc
    13621 agggtgtgca aattgtggtt taacccataa actgaaccag aaatggttaa tctatcggtt
    13681 aaacggtttt attgtttttt tttttttttn gatatattga tctttcagtt caatgattag
    13741 ttctttgagt ttacttgcat actaatttca tatttccctt gcataagttt cgttagcttg
    13801 actatgatat attagagttt gctataaggg aaaagatata caaatgtatc gttgtatctg
    13861 tggtaaaaag caagtacaat ctaatgtgtc atgtatcatt cgcatttcaa agtaaaacta
    13921 aggagtttag tctaggttga tattaataga ctgttgtttt gcattaattc tttgttgcgg
    13981 ttggcagtac caagcgttgg taagggaaat gattgaattc tattttactt gatatttttt
    14041 tctagttgga aactcaggaa caaatgtttt cttactagaa aaaataatta gaaacaaaag
    14101 cagcatatga ggaatcttca aatacatgtg aattagaact cttgacacca aaagtttcaa
    14161 cgcaacaagg agatgagcaa atgtgatctc cacatttgct tcctatatca ctctctcatt
    14221 acaagcagca aaccacaaca aaaaatttcg cctatgataa agtatgcttg atttcactcc
    14281 acaaggcatc ccaaagatgt tgatctagcc gtcactctaa gccaccaaac acagacacca
    14341 ttaatttatc atgatacttt tctggataaa ttctgaaaac cagagccaag acacatgaga
    14401 ttgacagttc ctgtggaggc atcaggtttc atgacaaact taacacccaa aaaatctctg
    14461 atgtgcctta atgctgctcc gagctcgaat tctagtagcg ttgtcaatca attggcaagt
    14521 cataaaatgc attaaaaaat attttcatac tcaactacaa atccatgagt ataactataa
    14581 ttataaagca atgattagaa tctgacaagg attctggaaa attacataaa ggaaagttca
    14641 taaatgtcta aaacacaaga ggacatactt gtattcagta acatttgcag cttttctagg
    14701 tctgaaaata tatttgttgc ctagtgaata agcataatgg tacaactaca agtgttttac
    14761 tcctcatatt aacttcggtc attagaggcc acgatttgac acatttttac tcaaaacaaa
    14821 atgtttgcat atctcttata atttcaaatt caacacacaa caaataagag aaaaaacaaa
    14881 taatattaat ttgagaatga acaaaaggac catatcattc attaactctt ctccatccat
    14941 ttccatttca cagttcgata gcgaaaaccg aataaaaaac acagtaaatt acaagcacaa
    15001 caaatggtac aagaaaaaca gttttcccaa tgccataata ctcaaactca gtaggattct
    15061 ggtgtgtgcg caatgaaact gatgcattga acttgacgaa cgttgtcgaa accgatgata
    15121 cgaacgaaag ctctaagctc agactttacg cttcttcttt gggtcagccc tagagtcacc
    15181 acccaactgc gacagatcta tccttgtctc atagagccct gtaatagatt ggtgaataag
    15241 ggtagcgtcc aacacttcct tggttgatgt gtagcgcttt ctgtcgatag tggtatcgaa
    15301 gtacttaaag gctgctgggg ctcccagatt ggtcagtgtg aacaggtgga tgatgttttc
    15361 cgcttgttcc cttatcggct tatctctgtg cttgttgtac gcggaaagca cctttaattg
    15421 tagaaaacca gtaaaacatg gacaattagg aggcatcgat tatctaacca aagactttaa
    15481 caacactaat ctgccactaa tattcatgag aagtccttac cttatcgagg ttagcgtctg
    15541 caagaatgac ccgcttggaa aattcgctga tctgctctat gatctcatca agatagtgct
    15601 tgtgctgctc gacaaacaac tgcttctgct cgttgtcctc tggtgacccc ttcagcttct
    15661 catagtgtga ggccaagtac aggaagttga cgtacttact gggcaatgcc agctcgttgc
    15721 ccttctgcag ttctccggca gatgccagca tgcgttttct gccattttcc aattcgaaaa
    15781 gcgaatactt gggcagtttg ataatgaggt ctttcttcac ctctttgtaa cctggtttgt
    15841 ataattttgg ttcagttaat taggaaaatt acataatggc tccaaaaaaa aattatcgac
    15901 cttttaccaa aaaaaataaa atttatcgac cttgacagga aaaagttaca aatcaaaaga
    15961 aagaatatcg tctggcatcc aatattttac ctttagcctc gaggaagtcg atcggattct
    16021 tctcgaacga ggacctttcc attatggtaa ttccaagcag ttccttgacg cttttcagtt
    16081 tcttgctctt gcccttctcc accttggcca ctaccagtac gctgtatgcc acggtagggc
    16141 tatcaaagcc tccgtatttc ttcggatccc aatccttctt cctcgcaatc agtttgtcgg
    16201 agttgcgctt agggagaatg gactccttgg agaaaccacc ggtttgaacc tccgttttct
    16261 tgactatgtt gacttggggc atgctgagaa ccttacgcac agtcgcaaaa tccctgccct
    16321 tatcccagac aatctcacct gcaagaaaca gcaaagagag agttaagata taagtgaaca
    16381 agaaagtctt gggatcgaca tacatacaat caattgatca aattcctagg attaaacata
    16441 tgaaatagga aagacagacc tgtttcgcca tttgtctcga tcagaggcct cttccggatt
    16501 tcaccattcg ccagagtgat ctctgtcttg aagaagttca tgatattcga gtaaaagaag
    16561 tatttcgcgg tagccttgcc gatctcttgt tctgacttcg cgatcatctt cctaacgtcg
    16621 tacaccttgt aatccccgta cacaaactca gactccagtt tcggatactt cttgatgaga
    16681 gcagtgccaa caacggcgtt caggtaggcg tcatgggcat gatggtagtt gttgatctcg
    16741 cgaaccttgt aaaactggaa gtccttccgg aagtcactaa ccagtttgct cttgagggtg
    16801 atcacctgcg taaccatcaa aagtcaatat ggattcatca caaattaatc aggaatagaa
    16861 agatcgggtg gccttgaagt tgatcaaata aaaagtttcg aactttagtt tgatatggta
    16921 aaggaaagtt ggaaacttta cctttacctc cctgataagt ttgtcattct catcatactt
    16981 cgtattcata cgcgaatcga gtatctgggc cacatgtttc gtaatctgac gagtttcaac
    17041 caactggcgc ttgatgaagc cggctttgtc gagctcagac agtccgcccc tttctgcctt
    17101 cgtgagattg tcgaatttcc gttgggttat gagcttagca ttcaaaagct gcctccaata
    17161 gttcttcatc ttcttcacaa cctcctcgct gggtacattg tcggattttc cgcggttctt
    17221 gtctgagcgc gtaaggacct ataacacggg agaaaccaaa aagcaaactc atttcacgcc
    17281 aaatactaca gatttctcaa aaattaagtt ataatccata tatcctgaca agaaattgat
    17341 taaaacacag ttgctttacc ttgttgtcaa tactatcgtc cttcaggaaa gattgaggga
    17401 caatgtgatc cacatcatag tcggagagtc tgttaatgtc gagctcttgg tcgacgtaca
    17461 tatctctccc attctggagg taatagaggt acagcttctc gttctggagt tgagtatttt
    17521 cgactgggtg ctctttcaga atctgactcc caagctcctt gatgccttcc tctatccgct
    17581 tcatgcgctc ccgtgagttc ttctgccctt tctgagtcgt ctggttttcc ctcgccatct
    17641 cgatgacgat gttctccggc ttatggcggc ccatgacctg tagcaataat taaaacaaaa
    17701 ataccacaaa cagttagaac tgaggcaaga agaatgaatg cagagaaaaa aaatcttagc
    17761 tattcatgga aatggaagtg cgacgaacac ctaaaatgca aggagcataa ccaaatgcag
    17821 aacttacctt caccagctca tccacaacct tcactgtctg cagtatgccc ttctttatag
    17881 ctggcgatcc agccaggttg gctatgtgtt cgtgaaggct atcgccctga cctgacactt
    17941 gggccttctg gatatcttct ttgaaggtca gtgagtcatc gtgaataagc tgcatgaagt
    18001 tccgattcgc aaaaccgtcg gatttgagaa agtccagtat agtcttgccg gattgcttgt
    18061 cgcggatgcc gttgatgagc ttcctggaga gccttcccca acctaaaaca tagaacaaac
    18121 atttgtctca tcacaaatat gcatcaagga acactcagaa gttagtcata tacccaaatt
    18181 cttgaaacta gagaaacaaa aaaagaggga tcattgcgga aaccttttga cctcttacct
    18241 gtatatctcc tcctcttcaa ctgcttcatg accttgtcat cgaacagatg cgcataagtc
    18301 ttaaggcgct cctcgatcat ttctctgtcc tcaaacagcg tgagagtcag cacgatgtcc
    18361 tcgaggatgt cctcgttctc ctcgttgtca aggaagtcct tgtccttgat gattttcaac
    18421 aggtcatggt atgtgccaag agaggcattg aacctatcct caacgccgct gatttccaca
    18481 gaatcaaagc attcgatctt cttaaagtag tcctctttaa gctgtttcac agtgaccttc
    18541 cgattggttt tgaacaagag atcgacaatg gccttcttct gctcacctgt tataacagta
    18601 aaaattactt tagaaacaaa acaaaagtgt ggtcttttag gaatgattat tgatcagttc
    18661 atagtttaaa atcttgtgtt tttatcgtaa aagtgtggac caggatttac ctgacaggaa
    18721 ggccggtttg cgcatcccct ccgtcacata tttcaccttc gtcagctcgt tgtagaccgt
    18781 gaagtattcg tacaggagac tatgcttggg caggactttc tcatttggga gattcttatc
    18841 gaagttggtc atcctttcga tgaaggactg tgcactcgca cccttgtcca cgacctcttc
    18901 gaagttccaa ggcgtgatag tctcctcgga tttgcgtgtc atccacgcaa accgcgagtt
    18961 cccgcgtgcg agtggaccta atgaattaat agaaaaatca ggcaaattag aaaagaaata
    19021 attctcaaaa gagaacaact gaggtcagaa aatagcattc atcaactgaa actaggtatt
    19081 tcttcatatg atactaacct acatagtagg gaatgcggaa cgtgaggatc ttttcaatct
    19141 tctctctgtt gtctttcagg aacggataga agtcctcttg cctcctcaag atagcatgca
    19201 gctcaccgag gtggatctgg tgcggtatgc tgccattgtc gaaggtacgc tgcttccgaa
    19261 gcaaatcttc gcggttcaat ttaacgagca gctcctctgt accgtccatc ttttccagaa
    19321 taggcttgat gaatttgtag aattcctcct gacttgcacc taataatatc atcagaggaa
    19381 aactcaattc ttatatctta cagaaatgaa caacaaaata catgtagcag agaggaaaac
    19441 aatcataagt aacaacttac ctccatcaat gtaccctgcg tagccattct tcgattggtc
    19501 aaagaaaatc tccttgtact tctcggggag ttgctggcga accaaagcct tcagaagggt
    19561 cagatcctgg tgatgctcgt cgtacctctt gatcatggag gcgctaagtg gcgctttggt
    19621 tatctccgtg ttgacccgca gaatgtcact gagcaagata gcgtctgaca gattcttggc
    19681 agccaagaac aggtcggcat actgatcacc tacaaaagtt taaaagactc aattttaact
    19741 ctacagcttc aaataaattt ccgcaaagaa attgataggt ttagacaaca aaagaagagt
    19801 tccaatttca agagcaccta tttgggcgag cagattgtcc agatcatcat cgtatgtatc
    19861 ctttgacagc tgcagcttcg cgtcctcggc gaggtcaaag ttgcttttga agttaggggt
    19921 gaggcccaga gacagtgcaa tcagattccc gaacaagccg ttcttcttct cgcctggcaa
    19981 ttgagcgatc agattctcaa gccgtctgga cttcgaaagt ctggcagaca gtattgcctt
    20041 ggcgtcgaca cctgagattg tttgcaaaga aaaatctcaa agcatgtaca atctataaac
    20101 ttatgatgga aaaagaaaag aaaggtaact aaggaatgtt acctgatgca ttgatggggt
    20161 tctcctcgaa cagctgattg taggtttgga cgagctgaat gaacagttta tccacatcgg
    20221 agttatccgg gttgaggtcc ccttcgatca agaaatggcc acgaaacttt atcatgtgcg
    20281 cgagagccag gtatatgagc cgaaggtctg ccttatcggt tgagtccacc agcttcttgc
    20341 gaaggtggta tatagtcgga tacttttcat gataggctac ttcgtcaaca atattcccaa
    20401 agatggggtg acgctcgtgt ttcttatctt cctctaccag gaaggattcc tcgaggcgat
    20461 ggaagaacga gtcgtcaacc tgcaaaatca agtttattga tcatcaagtg agtttacatg
    20521 tagttaaatg ctttcagtaa tatcccaatg aagcaggata tgctttcagt acaaaataca
    20581 taagcatact atatcataaa aatccttacc ttcgccatct cgttgctaaa gatctcttgg
    20641 agataacaga ttctgttctt cctgcgagtg taccttctcc tcgcagtacg tttaagcctt
    20701 gtcgcctccg cggtttctcc agagtcaaag agcagagctc cgatcaagtt cttcttaatg
    20761 ctgtgcctat ctgtgttgcc cagcacttta aatttcttcg aaggtacctt gtattcatcg
    20821 gtgataacgg cccacccaac tgagttcgta ccaatatcaa gtccaatgct gtacttcttg
    20881 tccattgcag atttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
    20941 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgagta
    21001 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
    21061 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
    21121 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
    21181 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
    21241 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
    21301 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
    21361 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
    21421 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
    21481 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
    21541 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
    21601 ctcactgcgg cagctgagtc gagtttaact cttttggcag caggagccat ggtatcgata
    21661 attgtaaatg taattgtaat gttgtttgtt gtttgttgtt gttggtaatt gttgtaaaaa
    21721 tgagctctta tactcgagcg tgtcctctcc aaatgaaatg aacttcctta tatagaggaa
    21781 gggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatgtcacat
    21841 caatccactt gctttgtaga cgtggttgga acctcttctt tttccacgat gctcctcgtg
    21901 ggtgggggtc catctttggg accactgtcg gcagagagat cttgaatgat agcctttcct
    21961 ttatcgcaat gatggcattt gtaggagcca ccttcctttt ctactgtcct ttcgatgaag
    22021 tgacagatag ctgggcaatg gaatccgagg aggtttcccg aaattatcct ttgttgaaaa
    22081 gtctcaatag ccctttgatc ttctgagact gtatctttga catttttgga gtagaccaga
    22141 gtgtcgtgct ccaccatgtt gacgaagatt ttcttcttgt cattgagtcg taaaagactc
    22201 tgtatgaact gttcgccagt cttgacggcg agttctgtta gatcctcgat ttgaatctta
    22261 gactccatgc atggccttag attcagtagg aactaccttt ttagagactc caatctctat
    22321 tacttgcctt ggtttatgaa gcaagccttg aatcgtccat actggaatag tacttctgat
    22381 cttgagaaat atgtctttct ctgtgttctt gatgcaatta gtcctgaatc ttttgactgc
    22441 atctttaacc ttcttgggaa ggtatttgat ctcctggaga ttgttactcg ggtagatcgt
    22501 cttgatgaga cttgctgcgt aggcctctct aaccatctgt gggtcagcat tctttctgaa
    22561 attgaagagg ctaaccttct cattatcagt ggtgaacata gtgtcgtcac cttcaccttc
    22621 gaacttcctt cctagatcgt aaagatagag gaaatcgtcc attgtaatct ccggggcaaa
    22681 ggagatctct tttggggctg gatcactgct gggccttttg gttcctagcg tgagccagtg
    22741 ggctttttgc tttggtgggc ttgttagggc cttagcaaag ctcttgggct tgagttgagc
    22801 ttctcctttg gggatgaagt tcaacctgtc tgtttgctga cttgttgtgt acgcgtcagc
    22861 tgctgctctt gcctctgtaa tagtggcaaa tttcttgtgt gcaactccgg gaacgccgtt
    22921 tgttgccgcc tttgtacaac cccagtcatc gtatataccg gcatgtggac cgttatacac
    22981 aacgtagtag ttgatatgag ggtgttgaat acccgattct gctctgagag gagcaactgt
    23041 gctgttaagc tcagattttt gtgggattgg aattcctcca agcttgaatt cttgcagaga
    23101 ccgagacgtt gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact
    23161 accgggcgta ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa
    23221 aaaaatcact ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga
    23281 ggcatttcag tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc
    23341 ctttttaaag accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct
    23401 tgcccgcctg atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt
    23461 gatatgggat agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc
    23521 atcgctctgg agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga
    23581 tgtggcgtgt tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt
    23641 tttcgtctca gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat
    23701 ggacaacttc ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt
    23761 gctgatgccg ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag
    23821 aatgcttaat gaattacaac agtactgcga tgagtggcag ggggggcgta aacgccgcgt
    23881 ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt
    23941 ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat gctatgaagc
    24001 agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt
    24061 caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga
    24121 acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa
    24181 cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt tacacctata
    24241 aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg
    24301 ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg
    24361 aactttaccc agtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg
    24421 ccagtgtgcc ggtttccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg
    24481 acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac
    24541 acagccagtc ggtctcagct tctgcaggca tgcgacgtcg agcttagctt gagcttggat
    24601 cagattgtcg tttcccgcct tcagtttaaa ctatcagtgt ttgacaggat atattggcgg
    24661 gtaaacctaa gagaaaagag cgtttattag aataatcgga tatttaaaag ggcgtgaaaa
    24721 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
    24781 agtactttaa agtactttaa agtactttaa agtactttga tccaacccct ccgctgctat
    24841 agtgcagtcg gcttctgacg ttcagtgcag ccgtgttctg aaaacgacat gtcgcacaag
    24901 tcctaagtta cgcgacaggc tgccgccctg ccctt
//
