LOCUS       sequence-31002-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte 
            EM, Voigt CA, Ellington AD
  TITLE     A synthetic genetic edge detection program.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1272-81.
  PUBMED    19563759
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1272-81."
COMMENT     Sequence Label: pJT103
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(172..276)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     341..359
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(363..406)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    409..430
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             547..558
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(581..603)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
       61 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
      121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
      181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
      241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttcccatgg tgccacctga
      301 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggca
      361 gaatttcaga taaaaaaaat ccttagcttt cgctaaggat gatttctgga attcgcggcc
      421 gcttctagag tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt
      481 aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta
      541 ctagagaaag aggagaaata ctagagatga tagatcccgt cgttttacaa cgtcgtgact
      601 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      661 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      721 gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt
      781 gcgatcttcc tgaggccgat actgtcgtcg tcccctcaac tggcagatgc acggttac
//
