LOCUS       sequence-31003-e         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte 
            EM, Voigt CA, Ellington AD
  TITLE     A synthetic genetic edge detection program.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1272-81.
  PUBMED    19563759
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1272-81."
COMMENT     Sequence Label: pJT104
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(171..275)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     340..358
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(362..405)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    408..429
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             535..546
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(569..591)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
       61 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
      121 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
      181 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
      241 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttcccatggt gccacctgac
      301 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggcag
      361 aatttcagat aaaaaaaatc cttagctttc gctaaggatg atttctggaa ttcgcggccg
      421 cttctagagt aagcacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag
      481 tcgaataaca ccgtgcgtgt tgactatttt acctctggcg gtgatatact ggagaaagag
      541 gagaaatact agagatgata gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      601 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      661 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
      721 ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
      781 a
//
