LOCUS       sequence-310031-         783 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses H2B-frFAST in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Li C, Tebo AG, Thauvin M, Plamont MA, Volovitch M, Morin X, Vriz S, 
            Gautier A
  TITLE     A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo 
            Molecular Imaging.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Jun 22. doi: 10.1002/anie.202006576.
  PUBMED    32568417
REFERENCE   2  (bases 1 to 783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Jun 22. doi: 10.1002/anie.202006576."
COMMENT     Sequence Label: pAG505-H2B-frFAST
FEATURES             Location/Qualifiers
     source          1..783
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      403..412
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             409..783
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNLFKDVAPGTVSSGFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKIHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atgcccgaac ctgcgaagtc agcgcccgct cccaaaaaag gctctaaaaa agctgtcgcc
       61 aagacccaga agaaggggga taagaaaagg cgtaagacca ggaaagagag ttacgccatt
      121 tacgtgtaca aagtactaaa acaagtccac ccggacactg gcatctcctc aaaggcgatg
      181 ggcattatga actcatttgt aaacgacatc ttcgagcgca tcgccggaga agcgtcgcgc
      241 ctggcgcatt acaacaagcg ctccactatc acatcccggg agatccagac ggccgtgcgc
      301 ctgctcctgc ccggagaact ggccaaacac gctgtgtctg agggcacaaa ggccgtgacc
      361 aagtacacca gctccaaggg cggaggctcc ggaggcggat ctgccaccat ggagcatgtt
      421 gcctttggca gtgaggacat cgagaacact ttggccaaaa tggacgacgg acaactggat
      481 gggttggcct ttggcgcaat tcagctcgat ggtgacggga atatcctgca gtacaatgct
      541 gctgaaggag acatcacagg cagagatccc aaacaggtga ttgggaagaa cttattcaag
      601 gatgttgcac ctggaacggt ttcctccggg ttttacggca aattcaagga aggcgtagcg
      661 tcagggaatc tgaacaccat gttcgaatgg atgataccga caagcagggg accaactaag
      721 gtcaagatac acatgaagaa agccctttcc ggtgacagct attgggtctt tgtgaaacgg
      781 gtg
//
