LOCUS       sequence-310035-        1650 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses MAP4-frFAST in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Li C, Tebo AG, Thauvin M, Plamont MA, Volovitch M, Morin X, Vriz S, 
            Gautier A
  TITLE     A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo 
            Molecular Imaging.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Jun 22. doi: 10.1002/anie.202006576.
  PUBMED    32568417
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Jun 22. doi: 10.1002/anie.202006576."
COMMENT     Sequence Label: pAG502-MAP4-frFAST
FEATURES             Location/Qualifiers
     source          1..1650
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1276..1650
                     /codon_start=1
                     /product="small protein tag, derived from photoactive 
                     yellow protein, that allows in vivo fluorescent labeling by
                     addition of a cell-permeant fluorogen (Plamont et al., 
                     2016)"
                     /label=Y-FAST
                     /translation="MEHVAFGSEDIENTLAKMDDGQLDGLAFGAIQLDGDGNILQYNAA
                     EGDITGRDPKQVIGKNLFKDVAPGTVSSGFYGKFKEGVASGNLNTMFEWMIPTSRGPTK
                     VKIHMKKALSGDSYWVFVKRV"
ORIGIN
        1 atggtgtccc ggcaagaaga agcaaaggct gctgtaggtg tgactggaaa tgacatcact
       61 accccgccaa acaaggagcc accaccaagc ccagaaaaga aagcaaagcc tttggccacc
      121 actcaacctg caaagacttc aacatcgaaa gccaaaacac agcccacttc tctccctaag
      181 caaccagctc ccaccacctc tggtgggttg aataaaaaac ccatgagcct cgcctcaggc
      241 tcagtgccag ctgccccaca caaacgccct gctgctgcca ctgctactgc caggccttcc
      301 accctacctg ccagagacgt gaagccaaag ccaattacag aagctaaggt tgccgaaaag
      361 cggacctctc catccaagcc ttcatctgcc ccagccctca aacctggacc taaaaccacc
      421 ccaaccgttt caaaagccac atctccctca actcttgttt ccactggacc aagtagtaga
      481 agtccagcta caactctgcc taagaggcca accagcatca agactgaggg gaaacctgct
      541 gatgtcaaaa ggatgactgc taagtctgcc tcagctgact tgagtcgctc aaagaccacc
      601 tctgccagtt ctgtgaagag aaacaccact cccactgggg cagcaccccc agcagggatg
      661 acttccactc gagtcaagcc catgtctgca cctagccgct cttctggggc tctttctgtg
      721 gacaagaagc ccacttccac taagcctagc tcctctgctc ccagggtgag ccgcctggcc
      781 acaactgttt ctgcccctga cctgaagagt gttcgctcca aggtcggctc tacagaaaac
      841 atcaaacacc agcctggagg aggccgggcc aaggtagaga aaaaaacaga ggcagctacc
      901 acagctggga agcctgaacc taatgcagtc actaaagcag ccggctccat tgcgagtgca
      961 cagaaaccgc ctgctgggaa agtccagata gtatccaaaa aagtgagcta cagtcatatt
     1021 caatccaagt gtgtttccaa ggacaatatt aagcatgtcc ctggatgtgg caatgttcag
     1081 attcagaaca agaaagtgga catatccaag gtctcctcca agtgtgggtc caaagctaat
     1141 atcaagcaca agcctggtgg aggagatgtc aagattgaaa gtcagaagtt gaacttcaag
     1201 gagaaggccc aagccaaagt gggaggcgga ttcgcggatc caccggtcgc caccatgagt
     1261 gtgattaaac cagacatgga gcatgttgcc tttggcagtg aggacatcga gaacactttg
     1321 gccaaaatgg acgacggaca actggatggg ttggcctttg gcgcaattca gctcgatggt
     1381 gacgggaata tcctgcagta caatgctgct gaaggagaca tcacaggcag agatcccaaa
     1441 caggtgattg ggaagaactt attcaaggat gttgcacctg gaacggtttc ctccgggttt
     1501 tacggcaaat tcaaggaagg cgtagcgtca gggaatctga acaccatgtt cgaatggatg
     1561 ataccgacaa gcaggggacc aactaaggtc aagatacaca tgaagaaagc cctttccggt
     1621 gacagctatt gggtctttgt gaaacgggtg
//
