LOCUS       sequence-310053-         553 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses eGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 553)
  AUTHORS   Gstalder C, Liu D, Miao D, Lutterbach B, DeVine AL, Lin C, Shettigar
            M, Pancholi P, Buchbinder EI, Carter SL, Manos MP, Rojas-Rudilla V, 
            Brennick R, Gjini E, Chen PH, Lako A, Rodig S, Yoon CH, Freeman GJ, 
            Barbie DA, Hodi FS, Miles W, Van Allen EM, Haq R
  TITLE     Inactivation of Fbxw7 impairs dsRNA sensing and confers resistance 
            to PD-1 blockade.
  JOURNAL   Cancer Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416.
  PUBMED    32371478
REFERENCE   2  (bases 1 to 553)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Discov. 2020 May 5. pii: 2159-8290.CD-19-1416. doi: 
            10.1158/2159-8290.CD-19-1416."
COMMENT     Sequence Label: pLenti6.2_3xFLAG_V5_ eGFP_V5
FEATURES             Location/Qualifiers
     source          1..553
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(63..104)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    146..170
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(232..253)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcaagtcgct tgggagcgcc tcccctaccc ggtaggtacc atcgtcatta ctaaccggta
       61 cgcgtagaat cgagaccgag gagagggtta gggataggct taccttcgaa ccgcgggccc
      121 tctagactcg agttactata gagcaaccac tttgtacaag aaagctgggt cggcgcgccc
      181 acccttggac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      241 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg actgggtgct
      301 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
      361 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcgcctt
      421 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
      481 cagcttgtgc tccatttagt taccgtcctc cttgaggtgg atgcccttcg gctcgatgcg
      541 gttcacgtgg gag
//
