LOCUS       sequence-310054-       13783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  cloning vector with regulatory regions of the murine Tdg gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB,
            Schar P, Schuermann D
  TITLE     Inducible TDG knockout models to study epigenetic regulation.
  JOURNAL   F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. 
            eCollection 2020.
  PUBMED    33082936
REFERENCE   2  (bases 1 to 13783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2020-09-9"; volume: "9:1112. doi"; pages: " 
            10.12688/f1000research.25637.2. eCollection 2020"
COMMENT     Sequence Label: pTCO2
FEATURES             Location/Qualifiers
     source          1..13783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(669..680)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5750..5783)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6271..6304)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(9333..9351)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(9334..9351)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9371..9388)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9377..9584
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(9628..9648)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(9628..9646)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9667..9683)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9667..9683)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9680..9702)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9691..9707
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9715..9721,9722..9739,9740..9745))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9760..9781
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9898..9915)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10069..10657)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10149..10168)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10828..11619,11620..11688))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11451..11470
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11689..11793)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11819..12274)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11956..11977)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12168..12187
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12401..12423
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12415..12432
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12416..12432
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12442..12461
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12442..12460
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    12491..12612
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12502..12521)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12556..12575
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(12834..13433)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(12917..12937)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     13414..13433
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(13443..13772)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      13457..13592
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     13757..13777
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tcgagatcga ttctcctggt tcctgcggtt ctttgaaact ttacacagag ccacacccag
       61 gcacagtacc tttgggtcat ggttttactg gaaaagagac ctccaatcac cttacttaat
      121 ctgagagtgt ttttgattgt gggttcatgc tttgattgtg ggttcatttt tgttaagtaa
      181 actgcaagaa ttttgaagca ttaaggtagc attgtgtaac aggaatcgtg ataacttttt
      241 tttttaactc tgggcacaaa attgctcagg attgggggtg gagggcattt gtcattaaac
      301 ctgactacac aattttgctc ccccagcccc acatgatgga agcagagata gctctgtaaa
      361 ttcttttctt acacacacac aggcacacac acatgctttt ttttaacttc atgataataa
      421 aaaaaattag ttttaactaa atggtattaa gtttgacata taataatctc acagaaagta
      481 tacatctgga agactatacc cacaactaag gcaatcaaca ttatgcccaa gttttctgtg
      541 actgttcatg tgtgggcaca tatgcgattt agaggtcaaa ggacaacttc aggagcattg
      601 aatctcaggc actgtccttt cctctctttt tccttttatt cctcttccaa actgggtgtc
      661 tcattgacct ggagctccca aaataggata ggttagctgc ccagtgaacc caagagatcg
      721 gcttgtctct gcttcactag agcttagccc aattggcgac ttccaggtac ctgtctcaga
      781 actaaacatg taaattaaac atggtggttt aaataggtat gagaaatggc tcaactttta
      841 agagtgggta gggcttttgc agaggactag aaggacactg gcaacactca ggaaactcac
      901 agccatttct agctccaaac ttctggatcc catgagcacc tgtgctcaca tgcctggacc
      961 cacacatccc ctcatgcatg taattcaaaa ttaaaaaaaa aaattcaaaa aggctgagca
     1021 tggtggcaca cacctttaat tccagttcta gggtagcaga ggcagggaga tctcagtaag
     1081 ttggacgaca gtctggtcta catataataa aattcctgaa cagccaagag tacataaaga
     1141 gactgtggtg gtggtttgaa taggcatggc ccccacagac tcaagggttt gaatgcttga
     1201 cccatggaga gtggcatgat tagaaggtgt ggcctattgg agtatatgtg gccttactgg
     1261 aggaggtgtc tcactgggct ttgaggtctt agatgttcca gccaggctgt gtctcactcc
     1321 cttcctgctg cctgccaatc agacatagaa ctctgagcta cctctccagc accatgcata
     1381 actcctgtgt gtcccagtgc tttctgccat gatgacagac taaacctctg aaactataag
     1441 ccagccccaa tgttttctta taagagttgc ttcggtcaca gtgtctcttc acagctatag
     1501 aaaccctaag acagtaagta aataagaata agatgcagag taactgagaa gttatttaat
     1561 gtcagcctct gacttccaca cttatctaca caggtacatg tacacccaaa cagacacata
     1621 catacaaaat acaaattaaa atgcatcata atagttcaaa ctggacttga gcttgcagtg
     1681 atcctcctgc ctcatcctct caactgctgg gcttataaga atataccacc atccactgct
     1741 ctggctcata gacttctgta tacttgtatc ctttggtgga gtatctgttc aaatctcttg
     1801 cccatttttt aagatctctt taatgtcatg catttgaatg ttttgcctac atgtatgtac
     1861 catgtgcatg cctgtgcact cagaggtcgg aagagggtgt tgaacactct ggaactggag
     1921 ttacaggtgg tgtgaaccac catgtggatg ctgggaactg aactccagtc ctttgcaaga
     1981 aaagcatgtg tgttaaacac actctccagc tcaatcgacc tgtttgtttt ttttttttaa
     2041 ctgtaaactg ttctttattt tggatacaag ccttttatca gatacactgc aaatgtctct
     2101 ttccaatcta tgctttatct tttccttagt agtataacga gacacttaaa aatttaaata
     2161 aattccaaat ttattacccc tccccaccct tttatgagac aaggtctctc tatcttagtt
     2221 agggtttgta ttgttctgaa gagacactaa atgttttctt ataagggaaa acatttaact
     2281 gggaatggcc tagtttcaga ggttcatcag agtcgggagg tagcttcttc ttcttgcatg
     2341 ctgccagaga agaagctgag agttctacac cttgatccac aggcaccaag agcctgttat
     2401 accacactga gcatagcttg agcatggaag acctcaaagc ctaccccaca gtaatacgct
     2461 ttctccaaca aggccacacc gactactcca ccaaggtcat acttcctaat agtgccactc
     2521 cttatgggcc aaacattcaa atacatggtc cgtctctata gggatcatgc ttaaaccacc
     2581 tcagtctatg tggtccaggc tggcctcaga ccctaggaac aaacagctga ggtgcatatc
     2641 ttacatacca gagcagacct ggcctccggg acctcctgca tccctcaggc cctatctcga
     2701 atccctgctc tgaactttcc agcccagggg ctgggcggcc cttcccacag aggctctttg
     2761 ctacataacc cagacatttt ggtttcttct cttcttcttc ttcttcctcc tccttttcct
     2821 tctctcatgc gaacacactt tctctctttc tcttcccccc tcctctctcc ttccccatgg
     2881 caacttcccc tgcctccttc cttgggagca gtaaacttac caggagagca gcttcccaat
     2941 aaaccagaag ttatatgcat tttaatctgg tttgaattgg ctcagttcac cagtggagaa
     3001 aaaaacttat cagtgtgact aaaagtgtgt gccaccatag cccagccctt ttggaaaata
     3061 aaagttttga attacataca tatggggatg tatgggtcca tgtgtgtgag cacaggtgcc
     3121 catggaatcc attagtttgg agctagtgat ggctgtgagc tgcctaagtg aatgctgtct
     3181 caactcaacg tccttctagt cctctgcaag agtcccacct actcttacaa gctgagccat
     3241 ttctccaacc ccaaagttgt ttcttttcta agtagagttt tcactgtttt atatgtattt
     3301 tactttatgc atctatgtgg ctttgcttgc aggttgttgt gcccctgctt tcaggtctag
     3361 tgccccagga aataagacaa ggatgtcata cccctcagga ctggagttag agacagttag
     3421 ttgtgagtca ccatatggga atcgaagcca ggtcctctgg aagagtagct ggtgttctta
     3481 acagctgagc catcactcca gctgccgata tgcttccctt taaaatatcc taggattaaa
     3541 aatatataca tatggagcac aaagccctgg ccatctctga gctcacatca cagaagggag
     3601 agatcattgt aatttaacca acgcctcttg tgaaacaaac caggcaatct aattcagtcg
     3661 ggcttctcac cattttctgc cacgtaacct aaagaaacag ggaagcctaa cactcgaaga
     3721 tgtcagtgtt ctggattcct cgggacaatg gaagagagac atggtgggat gagtgatcag
     3781 cctctcaagg actgaatggt ggcaagtttc ctggaagtgc ccccattatt tatttattta
     3841 tttgctttac atcccagtca acctcccctc acaagtccct cccattactc cctcccttct
     3901 cctcatagta cagtcacttg acaacattca cttaggtgat acccaaagtg agtgctacca
     3961 ccccatcttg ggacatcaag tcatagaagg actaatcata gcctctctca ctgagcccaa
     4021 ccaggcagtc cagctagagg aaggggatcc aagaacaggt gggggctcac ataaagacca
     4081 ggcttcaatc tgctacaaat gtgtaggaag cgtaggtcaa gccctgcacg ctctttggtg
     4141 ggtggttcag tctctgtgag cccccatggg cccaggttag ttgactctgt aggtcttctt
     4201 gtggtgtcct tgctcacttc tatccctcac ttttccacag gactcccaaa cactgctgag
     4261 ccatcttacc agccccctag aagtgttgtt tattgttaca taaaaggcac ttttagcaag
     4321 ttaacttctg catacccaaa cctcttctat gatctagggg ttatgaagga gccattgccc
     4381 aagattctca gacacaggag actacctggt ctgccaggta cgtctaagga ctgatactgt
     4441 aaaacccttt gaaggaacac ccccctcaaa tctcagataa caatcgctga caagaggcta
     4501 cctcaaagcc taggtgccat cactctggga ttcatggaga gggctagacg ccattagaca
     4561 actcttctgg ccaaacaccc acagcagaca ttgaaaagaa attaaatgaa aactcttggg
     4621 gctggagaga tggctcagcg gttaagaaca ctgactgctc tttcgaaggt cctgagttca
     4681 aatcccagca accacatagt ggctcacaac catccgtaat gagatctgac tgccctcttc
     4741 tggtgtgtct gaagacagct acagtgtact cacatataaa taaataaata aataaataaa
     4801 taaataaata aatcttaaaa aaaaaaaaaa aaaaaacctc ttaaaacctc cgaaagtcaa
     4861 gtatggtgat acatgccctg taatcccagt cagtacgcaa gagagtgaac aggagacttg
     4921 cctcaggttg aggccagcct ggactacagc tgcaaagctc taagccaagt caggggtaca
     4981 cagttttgtg attatctcaa aaaccaaaag aacctctgag aaatcgaggt gagattggtg
     5041 tctaagattc taaagcagag atgcatctag catctaggct agtgtttgtc catctccaac
     5101 gtagacaaca gtttatggaa atctttgtag tctaccaaga gcttcccctt acagattagt
     5161 aaactgaggc ccaaacattg gtggcttcca atcttaggtt ttccagttcc aatcagccct
     5221 actcctcatc acaggaattg acaagaaaga caattgaaaa gaaagaatgt tgttttgtgt
     5281 aaggcacaat atttatcatt attataatta tcattttagt ttttcacgac ggagtttctc
     5341 tgtgtagtat tgcctgtccg gaaactcgct ctataaacta ggccagcctc gaacttagtt
     5401 ctgcctgcca cagtctttgc aaatgctggg tcgcccagcc aagacacata gttgcggggg
     5461 agggggagtt gttttcattt taatttaatt aactttacgt cccgttcaca gccccacccc
     5521 cacccagtcc cacgcttaca aatcctgccc ccttctcttc tcagagaaga ggaagcccca
     5581 cttgggtaag actctgggac atctaatctc agcaagtgta ggcacatcct ttcccactga
     5641 ggaaacacgc agtcctcttc ccccagtccg cgctgggtcc actggcaagg aacagagacc
     5701 tagacagccc ctgttccact tgtaggggac tcacacggac accaagctga taacttcgta
     5761 tagcatacat tatacgaagt tataagcttc acatttgcta caaacgtgta gaggggtcta
     5821 ggtccagctc ctgaaagctc cctggttggt agctcaggct ctgtgagccc caagtcaggt
     5881 agcttttaat ccgcacaaaa tttggggtga aaagacatga atttgccacc acgcataata
     5941 catcctaaag gccatcaccc ttaacccaca ccttcaagga aacttagagc ccacaaaagc
     6001 tgacgtcagc cgggcgctcc ccatcctctc cgagatccac gtttcccgaa aggctaagcg
     6061 gcggtcgggg cgccccgaaa aggacacgcc attggcctgg ccagcatcac gtgatggcga
     6121 ggccgccggc ctcctcggag ggggacggaa gtgcgctggg gactcgccgc ggtgatgtag
     6181 cagcgtggga ggggccgagg ccagcgccgt ccatccagcg gccgcgtcga cgaattcctg
     6241 cagcccgggg gatccttaag ctagctcgag ataacttcgt atagcataca ttatacgaag
     6301 ttatatcgag gaagggtgcc tctcagctcg ggctcagggc tgcagttcta atgcagacct
     6361 caggacggag agcctcagtg tgctaactga ggggctttag tgttaatggt cctttacgct
     6421 agtggtgtaa ttatatatgg agcagttata tggtagagtc aactgtatga atctgtgtag
     6481 tttggaaagg aaaaataggg tgttttgtac ttgagttttg tattttaact acccatcatt
     6541 ccagcttttt atatactata tttcatttat gaagaaactg gttttctctg aggagtcact
     6601 tcagtctgta attttaaaaa agcaacagtc ggaatattta tagttgattc ttcactgtgc
     6661 atacctatat atgcccttat ccctttaaat gcctaagtag ggcatgctac taagaatcaa
     6721 gctggtgaaa ggcagatgtg cctatctctg ttggaagccg actaaccctc cgccctccgt
     6781 ggactcgaag ctggaggtgg actcagaacc ctctgggttg cagtcacacg cagaccccct
     6841 tactctccca gtagtgtgtg tggcagttgc ttggtagtag aaacctggtg gggcctgagc
     6901 cctgccgttg gcctctgcct cttgtggttc ctgagaaggc agtagaacct ggacaattgc
     6961 cacccctctg ttttactgaa tcccccgtca gtgttcatca ctgttgtgat tttatcccga
     7021 catggaaagc gctaagaaat gaactttcca gttagccacg cgctgctgca ggtgtttgat
     7081 aaccctgttt aaactggtgc tgtgtacatg ggatgtactg atacagaact tctcttctag
     7141 ggaaagctaa tcatttgtca atgatttaaa aattattgtc actactgttg attactgtgg
     7201 actaagtgag cctcgtgaaa gagctgattg gaatttgtac ctttgtggct gctcatgcat
     7261 tttccatggt gctacagaca gcctgcccgg gctggtggct aggaaacgtg cgtgttcagc
     7321 gatgcctagc gctctcactc ctgagcactc tgacttggtg cccacccctg gcgcgtgctg
     7381 gtttggcagt atgaaatgcc cacgagggtt ttgtatctca caaaggttat gtagcctgtt
     7441 ctgtgaataa tgagggaagc agtttcttgg tggaaatttt aacatgcctt tagctgtggc
     7501 agtgatgggc tttctttttc cttctgtctg ggtcaggttg tgcacaagtc atgttatttg
     7561 gaaggtgcac tgcactgctg tttacatggg cattttatgc gggtgctttg aagtgccttg
     7621 catcagggat tagaatcaat tcaattcttt ttcatgggac tatgcaaagc atgtagtatt
     7681 gccttggtat gtaactattg tagttttacc atggagaaat acctttaaat tataactctg
     7741 gagagcagcc atgaggaccc tctgtacttc ctggtgcttt tccttaacat ggtgggtgag
     7801 tagcgagtgc ggggcaccct gtacatggca gggtggggcg ttctcattaa ataatactga
     7861 gattttaaac atgcgcagtc tgagcgtttt tacaataaac attaagaatg gctatgctta
     7921 gtatacaggt tttcctccag caggatttaa tggtattcct ccccactgga aacctgagga
     7981 tggcttactt tctcttacat gtcacatgac aaaaatcaca aaagcttgaa tagtttattt
     8041 ttacagattc tacatttttt taatttataa ttttgtaaaa gacaagacta gcaacctgtt
     8101 tttaagaaag aaaaccctgc agccagatgt ggtggcccag gccttcagtt ccagtactta
     8161 ggaggcaaag acatgaggat ctttgtgagt ttgaagttgt gagttccaat ttatccaggg
     8221 atatagagaa agaccctgtt tgtttgtttg tttgtttttg aggcagcgtt tctctccgta
     8281 gccttggctg tcctgaaact cactgtatag accaggctgg cctcgaactc acagattaaa
     8341 ctctttctgc ctcctgagtg ctgagattaa aggtatgtgc tgccactacc cggctaactt
     8401 gtttctatta ttttgaaaac atgtatggat attttgcctt ccatatgtgc atcacatgtg
     8461 tacccagtgc ctgtgaaggc cagaagagaa tgtcagagcc cctggaactg ccatgtgggt
     8521 tctgagaatc aaagctgggt cctctggaag agcagccaac actctctcca gccagggaga
     8581 tcccatctta gaagtaaaac aaaagaactg ctcccaaaat atctaactga gaaaatagag
     8641 attttgaaaa ttttcagata tagttgtcat tcaggttgag acttggggta catagcgaga
     8701 ccctgtctca aaacaccaaa agaaaagaat gagtaggggc tgctcatggc aatcgtttga
     8761 acttcaagaa gttaagtatc tgtgtgggcc ttggttcaac ttgtacacag tagtatttgt
     8821 ctcttacacc ataggtcata tatatcagaa gagttgctcc taaagaacag tgttagaaga
     8881 tacagagggc ccgttccatt tttatccagg gtgtatttta actgttggag tgtttcatct
     8941 attgtgatgc agtttaaaga tccctgcagg gtcaggaaca aaccagcttt cccataagtc
     9001 attgtgaaca ctaggaaagt cccagggttt atgtcttcca ttccagtgga gtagtttagc
     9061 ttcctgcagg aagtgctccg aatatcttgc atcagggttc cagccttcgg tgtgtgtgga
     9121 gggaaataaa caacaggctt gttagtgacg acgattttat atggaacctt ggatacaagc
     9181 tatggtagaa gtgtctggtt agcaggagtc acgcctcaca taagccccct tgttgataac
     9241 catacattac ataagcctaa ggttatgata gtgaagagat cttaatactg ccattcctcg
     9301 gagtgttcta cagtacatct agagggccct attctatagt gtcacctaaa tgctagagct
     9361 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     9421 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     9481 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     9541 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     9601 tcgccgagct gggcccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     9661 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     9721 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     9781 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     9841 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     9901 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     9961 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
    10021 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
    10081 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
    10141 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
    10201 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
    10261 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
    10321 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
    10381 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
    10441 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
    10501 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
    10561 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
    10621 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
    10681 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
    10741 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
    10801 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
    10861 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
    10921 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
    10981 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
    11041 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
    11101 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
    11161 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
    11221 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
    11281 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    11341 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    11401 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    11461 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    11521 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    11581 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    11641 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    11701 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    11761 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
    11821 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
    11881 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
    11941 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
    12001 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
    12061 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
    12121 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
    12181 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
    12241 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
    12301 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    12361 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    12421 acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg tacccggtat
    12481 acagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
    12541 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
    12601 aataaacaag ttggggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat
    12661 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga
    12721 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga actcgacgat
    12781 gcatggggtc gtgcgctcct ttcggtcggg cgctgcgggt cgtggggcgg gcgtcaggca
    12841 ccgggcttgc gggtcatgca ccaggtgcgc ggtccttcgg gcacctcgac gtcggcggtg
    12901 acggtgaagc cgagccgctc gtagaagggg aggttgcggg gcgcggaggt ctccaggaag
    12961 gcgggcaccc cggcgcgctc ggccgcctcc actccgggga gcacgacggc gctgcccaga
    13021 cccttgccct ggtggtcggg cgagacgccg acggtggcca ggaaccacgc gggctccttg
    13081 ggccggtgcg gcgccaggag gccttccatc tgttgctgcg cggccagccg ggaaccgctc
    13141 aactcggcca tgcgcgggcc gatctcggcg aacaccgccc ccgcttcgac gctctccggc
    13201 gtggtccaga ccgccaccgc ggcgccgtcg tccgcgaccc acaccttgcc gatgtcgagc
    13261 ccgacgcgcg tgaggaagag ttcttgcagc tcggtgaccc gctcgatgtg gcggtccgga
    13321 tcgacggtgt ggcgcgtggc ggggtagtcg gcgaacgcgg cggcgagggt gcgtacggcc
    13381 ctggggacgt cgtcgcgggt ggcgaggcgc accgtgggct tgtactcggt catggtaagc
    13441 tttttgcaaa agcctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
    13501 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
    13561 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gactatggtt
    13621 gctgactaat tgagatgcat gctttgcata cttctgcctg ctggggagcc tggggacttt
    13681 ccacacctgg ttgctgacta attgagatgc atgctttgca tacttctgcc tgctggggag
    13741 cctggggact ttccacaccc taactgacac acattccaca ggg
//
