LOCUS       sequence-310062-       15687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniTdg gene, SUMOylation mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15687)
  AUTHORS   Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB,
            Schar P, Schuermann D
  TITLE     Inducible TDG knockout models to study epigenetic regulation.
  JOURNAL   F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. 
            eCollection 2020.
  PUBMED    33082936
REFERENCE   2  (bases 1 to 15687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2020-09-9"; volume: "9:1112. doi"; pages: " 
            10.12688/f1000research.25637.2. eCollection 2020"
COMMENT     Sequence Label: pTCO2-mTDGi.2
FEATURES             Location/Qualifiers
     source          1..15687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(669..680)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(5750..5783)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      6308..6317
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          6337..6909
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     protein_bind    complement(8175..8208)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(11237..11255)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(11238..11255)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(11275..11292)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11281..11488
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(11532..11552)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(11532..11550)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11571..11587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11571..11587)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11584..11606)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11595..11611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11619..11625,11626..11643,11644..11649))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11664..11685
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11802..11819)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11973..12561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12053..12072)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12732..13523,13524..13592))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13355..13374
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13593..13697)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(13723..14178)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13860..13881)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     14072..14091
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     14305..14327
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14319..14336
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14320..14336
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     14346..14365
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        14346..14364
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    14395..14516
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14406..14425)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     14460..14479
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(14738..15337)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(14821..14841)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     15318..15337
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     promoter        complement(15347..15676)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15361..15496
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     15661..15681
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 tcgagatcga ttctcctggt tcctgcggtt ctttgaaact ttacacagag ccacacccag
       61 gcacagtacc tttgggtcat ggttttactg gaaaagagac ctccaatcac cttacttaat
      121 ctgagagtgt ttttgattgt gggttcatgc tttgattgtg ggttcatttt tgttaagtaa
      181 actgcaagaa ttttgaagca ttaaggtagc attgtgtaac aggaatcgtg ataacttttt
      241 tttttaactc tgggcacaaa attgctcagg attgggggtg gagggcattt gtcattaaac
      301 ctgactacac aattttgctc ccccagcccc acatgatgga agcagagata gctctgtaaa
      361 ttcttttctt acacacacac aggcacacac acatgctttt ttttaacttc atgataataa
      421 aaaaaattag ttttaactaa atggtattaa gtttgacata taataatctc acagaaagta
      481 tacatctgga agactatacc cacaactaag gcaatcaaca ttatgcccaa gttttctgtg
      541 actgttcatg tgtgggcaca tatgcgattt agaggtcaaa ggacaacttc aggagcattg
      601 aatctcaggc actgtccttt cctctctttt tccttttatt cctcttccaa actgggtgtc
      661 tcattgacct ggagctccca aaataggata ggttagctgc ccagtgaacc caagagatcg
      721 gcttgtctct gcttcactag agcttagccc aattggcgac ttccaggtac ctgtctcaga
      781 actaaacatg taaattaaac atggtggttt aaataggtat gagaaatggc tcaactttta
      841 agagtgggta gggcttttgc agaggactag aaggacactg gcaacactca ggaaactcac
      901 agccatttct agctccaaac ttctggatcc catgagcacc tgtgctcaca tgcctggacc
      961 cacacatccc ctcatgcatg taattcaaaa ttaaaaaaaa aaattcaaaa aggctgagca
     1021 tggtggcaca cacctttaat tccagttcta gggtagcaga ggcagggaga tctcagtaag
     1081 ttggacgaca gtctggtcta catataataa aattcctgaa cagccaagag tacataaaga
     1141 gactgtggtg gtggtttgaa taggcatggc ccccacagac tcaagggttt gaatgcttga
     1201 cccatggaga gtggcatgat tagaaggtgt ggcctattgg agtatatgtg gccttactgg
     1261 aggaggtgtc tcactgggct ttgaggtctt agatgttcca gccaggctgt gtctcactcc
     1321 cttcctgctg cctgccaatc agacatagaa ctctgagcta cctctccagc accatgcata
     1381 actcctgtgt gtcccagtgc tttctgccat gatgacagac taaacctctg aaactataag
     1441 ccagccccaa tgttttctta taagagttgc ttcggtcaca gtgtctcttc acagctatag
     1501 aaaccctaag acagtaagta aataagaata agatgcagag taactgagaa gttatttaat
     1561 gtcagcctct gacttccaca cttatctaca caggtacatg tacacccaaa cagacacata
     1621 catacaaaat acaaattaaa atgcatcata atagttcaaa ctggacttga gcttgcagtg
     1681 atcctcctgc ctcatcctct caactgctgg gcttataaga atataccacc atccactgct
     1741 ctggctcata gacttctgta tacttgtatc ctttggtgga gtatctgttc aaatctcttg
     1801 cccatttttt aagatctctt taatgtcatg catttgaatg ttttgcctac atgtatgtac
     1861 catgtgcatg cctgtgcact cagaggtcgg aagagggtgt tgaacactct ggaactggag
     1921 ttacaggtgg tgtgaaccac catgtggatg ctgggaactg aactccagtc ctttgcaaga
     1981 aaagcatgtg tgttaaacac actctccagc tcaatcgacc tgtttgtttt ttttttttaa
     2041 ctgtaaactg ttctttattt tggatacaag ccttttatca gatacactgc aaatgtctct
     2101 ttccaatcta tgctttatct tttccttagt agtataacga gacacttaaa aatttaaata
     2161 aattccaaat ttattacccc tccccaccct tttatgagac aaggtctctc tatcttagtt
     2221 agggtttgta ttgttctgaa gagacactaa atgttttctt ataagggaaa acatttaact
     2281 gggaatggcc tagtttcaga ggttcatcag agtcgggagg tagcttcttc ttcttgcatg
     2341 ctgccagaga agaagctgag agttctacac cttgatccac aggcaccaag agcctgttat
     2401 accacactga gcatagcttg agcatggaag acctcaaagc ctaccccaca gtaatacgct
     2461 ttctccaaca aggccacacc gactactcca ccaaggtcat acttcctaat agtgccactc
     2521 cttatgggcc aaacattcaa atacatggtc cgtctctata gggatcatgc ttaaaccacc
     2581 tcagtctatg tggtccaggc tggcctcaga ccctaggaac aaacagctga ggtgcatatc
     2641 ttacatacca gagcagacct ggcctccggg acctcctgca tccctcaggc cctatctcga
     2701 atccctgctc tgaactttcc agcccagggg ctgggcggcc cttcccacag aggctctttg
     2761 ctacataacc cagacatttt ggtttcttct cttcttcttc ttcttcctcc tccttttcct
     2821 tctctcatgc gaacacactt tctctctttc tcttcccccc tcctctctcc ttccccatgg
     2881 caacttcccc tgcctccttc cttgggagca gtaaacttac caggagagca gcttcccaat
     2941 aaaccagaag ttatatgcat tttaatctgg tttgaattgg ctcagttcac cagtggagaa
     3001 aaaaacttat cagtgtgact aaaagtgtgt gccaccatag cccagccctt ttggaaaata
     3061 aaagttttga attacataca tatggggatg tatgggtcca tgtgtgtgag cacaggtgcc
     3121 catggaatcc attagtttgg agctagtgat ggctgtgagc tgcctaagtg aatgctgtct
     3181 caactcaacg tccttctagt cctctgcaag agtcccacct actcttacaa gctgagccat
     3241 ttctccaacc ccaaagttgt ttcttttcta agtagagttt tcactgtttt atatgtattt
     3301 tactttatgc atctatgtgg ctttgcttgc aggttgttgt gcccctgctt tcaggtctag
     3361 tgccccagga aataagacaa ggatgtcata cccctcagga ctggagttag agacagttag
     3421 ttgtgagtca ccatatggga atcgaagcca ggtcctctgg aagagtagct ggtgttctta
     3481 acagctgagc catcactcca gctgccgata tgcttccctt taaaatatcc taggattaaa
     3541 aatatataca tatggagcac aaagccctgg ccatctctga gctcacatca cagaagggag
     3601 agatcattgt aatttaacca acgcctcttg tgaaacaaac caggcaatct aattcagtcg
     3661 ggcttctcac cattttctgc cacgtaacct aaagaaacag ggaagcctaa cactcgaaga
     3721 tgtcagtgtt ctggattcct cgggacaatg gaagagagac atggtgggat gagtgatcag
     3781 cctctcaagg actgaatggt ggcaagtttc ctggaagtgc ccccattatt tatttattta
     3841 tttgctttac atcccagtca acctcccctc acaagtccct cccattactc cctcccttct
     3901 cctcatagta cagtcacttg acaacattca cttaggtgat acccaaagtg agtgctacca
     3961 ccccatcttg ggacatcaag tcatagaagg actaatcata gcctctctca ctgagcccaa
     4021 ccaggcagtc cagctagagg aaggggatcc aagaacaggt gggggctcac ataaagacca
     4081 ggcttcaatc tgctacaaat gtgtaggaag cgtaggtcaa gccctgcacg ctctttggtg
     4141 ggtggttcag tctctgtgag cccccatggg cccaggttag ttgactctgt aggtcttctt
     4201 gtggtgtcct tgctcacttc tatccctcac ttttccacag gactcccaaa cactgctgag
     4261 ccatcttacc agccccctag aagtgttgtt tattgttaca taaaaggcac ttttagcaag
     4321 ttaacttctg catacccaaa cctcttctat gatctagggg ttatgaagga gccattgccc
     4381 aagattctca gacacaggag actacctggt ctgccaggta cgtctaagga ctgatactgt
     4441 aaaacccttt gaaggaacac ccccctcaaa tctcagataa caatcgctga caagaggcta
     4501 cctcaaagcc taggtgccat cactctggga ttcatggaga gggctagacg ccattagaca
     4561 actcttctgg ccaaacaccc acagcagaca ttgaaaagaa attaaatgaa aactcttggg
     4621 gctggagaga tggctcagcg gttaagaaca ctgactgctc tttcgaaggt cctgagttca
     4681 aatcccagca accacatagt ggctcacaac catccgtaat gagatctgac tgccctcttc
     4741 tggtgtgtct gaagacagct acagtgtact cacatataaa taaataaata aataaataaa
     4801 taaataaata aatcttaaaa aaaaaaaaaa aaaaaacctc ttaaaacctc cgaaagtcaa
     4861 gtatggtgat acatgccctg taatcccagt cagtacgcaa gagagtgaac aggagacttg
     4921 cctcaggttg aggccagcct ggactacagc tgcaaagctc taagccaagt caggggtaca
     4981 cagttttgtg attatctcaa aaaccaaaag aacctctgag aaatcgaggt gagattggtg
     5041 tctaagattc taaagcagag atgcatctag catctaggct agtgtttgtc catctccaac
     5101 gtagacaaca gtttatggaa atctttgtag tctaccaaga gcttcccctt acagattagt
     5161 aaactgaggc ccaaacattg gtggcttcca atcttaggtt ttccagttcc aatcagccct
     5221 actcctcatc acaggaattg acaagaaaga caattgaaaa gaaagaatgt tgttttgtgt
     5281 aaggcacaat atttatcatt attataatta tcattttagt ttttcacgac ggagtttctc
     5341 tgtgtagtat tgcctgtccg gaaactcgct ctataaacta ggccagcctc gaacttagtt
     5401 ctgcctgcca cagtctttgc aaatgctggg tcgcccagcc aagacacata gttgcggggg
     5461 agggggagtt gttttcattt taatttaatt aactttacgt cccgttcaca gccccacccc
     5521 cacccagtcc cacgcttaca aatcctgccc ccttctcttc tcagagaaga ggaagcccca
     5581 cttgggtaag actctgggac atctaatctc agcaagtgta ggcacatcct ttcccactga
     5641 ggaaacacgc agtcctcttc ccccagtccg cgctgggtcc actggcaagg aacagagacc
     5701 tagacagccc ctgttccact tgtaggggac tcacacggac accaagctga taacttcgta
     5761 tagcatacat tatacgaagt tataagcttc acatttgcta caaacgtgta gaggggtcta
     5821 ggtccagctc ctgaaagctc cctggttggt agctcaggct ctgtgagccc caagtcaggt
     5881 agcttttaat ccgcacaaaa tttggggtga aaagacatga atttgccacc acgcataata
     5941 catcctaaag gccatcaccc ttaacccaca ccttcaagga aacttagagc ccacaaaagc
     6001 tgacgtcagc cgggcgctcc ccatcctctc cgagatccac gtttcccgaa aggctaagcg
     6061 gcggtcgggg cgccccgaaa aggacacgcc attggcctgg ccagcatcac gtgatggcga
     6121 ggccgccggc ctcctcggag ggggacggaa gtgcgctggg gactcgccgc ggtgatgtag
     6181 cagcgtggga ggggccgagg ccagcgccgt ccatccagcg gccgccccga tcctgtggtg
     6241 agtccgcgtg ctgagcggca gagcgggcgc ccgtgggctc ggcaggagtg gccgtaccct
     6301 cggcggcgcc gccatggacg cagaggccgc gcgcaggtga gtttggggac ccttgattgt
     6361 tctttctttt tcgctattgt aaaattcatg ttatatggag ggggcaaagt tttcagggtg
     6421 ttgtttagaa tgggaagatg tcccttgtat caccatggac cctcatgata attttgtttc
     6481 tttcactttc tactctgttg acaaccattg tctcctctta ttttcttttc attttctgta
     6541 actttttcgt taaactttag cttgcatttg taacgaattt ttaaattcac ttttgtttat
     6601 ttgtcagatt gtaagtactt tctctaatca cttttttttc aaggcaatca gggtatatta
     6661 tattgtactt cagcacagtt ttagagaaca attgttataa ttaaatgata aggtagaata
     6721 tttctgcata taaattctgg ctggcgtgga aatattctta ttggtagaaa caactacatc
     6781 ctggtcatca tcctgccttt ctctttatgg ttacaatgat atacactgtt tgagatgagg
     6841 ataaaatact ctgagtccaa accgggcccc tctgctaacc atgttcatgc cttcttcttt
     6901 ttcctacagc tattctctgg agcaagttca agctttgtat tcatttccat ttcaacaaat
     6961 gatggcagaa gttcctaaca tggcagtcac gactggacag caggtgccag cagtagctcc
     7021 taacatggca accgtgacag aacagcaggt gcccgcagac gctcctgtcc aggaacctgc
     7081 accagaagct ccaaagagaa ggaaaaggaa acccagagca gcagagcccc aggaaccagt
     7141 ggagcccaaa aaacctgcta cgtcgaagaa atccggcaag tctacaaaat caaaggaaaa
     7201 gcaggagaaa atcacagacg cgtttaaagt gaaaaggaaa gtggaccgct tcaacggcgt
     7261 ctctgaagct gagcttctga ccaagactct tcctgacatt ttgaccttca atctggatat
     7321 tgtgatcatt ggcattaacc cgggattaat ggctgcttac aaaggacatc actaccctgg
     7381 gcctggaaat cacttctgga agtgtctgtt catgtcgggg ctgagtgagg tgcagctgaa
     7441 tcacatggat gaccacacct tacccggcaa gtacggcatc ggattcacca acatggtgga
     7501 acggacgacg ccgggcagca aggatctgtc tagtaaagag ttccgggaag gagggcgcat
     7561 cctggtgcag aaactgcaga aatatcagcc acgaatagcg gtgtttaatg gaaaatgtat
     7621 ttatgaaatt ttcagtaaag aagtttttgg agtaaaggtt aagaacttgg aatttgggct
     7681 tcaaccccac aagatcccag acacagaaac tctgtgctac gtcatgccgt cgtccagcgc
     7741 cagatgtgct cagtttcccc gggcccagga caaagttcat tactacatta agctgaagga
     7801 cttgagagac cagctgaaag gcattgaacg caacaccgac gttcaggaag tgcagtatac
     7861 atttgacctg cagcttgcgc aagaggacgc aaagaagatg gctgttaggg aagaaaagta
     7921 tgatccaggc tatgaggcag cttacggcgg cgcctatggg gaaaacccat gtaatgggga
     7981 accttgtggc attgcttcaa atgggctaac agcccacagt gcggagccga gaggagaagc
     8041 gacccccggc gatgttccaa atgggcagtg gatggcacag tcgtttgcag agcagatccc
     8101 ttcttttaat aattgtggga cccgagagca ggaagaagag agccacgctt agtgaagatc
     8161 taagctagct cgagataact tcgtatagca tacattatac gaagttatat cgaggaaggg
     8221 tgcctctcag ctcgggctca gggctgcagt tctaatgcag acctcaggac ggagagcctc
     8281 agtgtgctaa ctgaggggct ttagtgttaa tggtccttta cgctagtggt gtaattatat
     8341 atggagcagt tatatggtag agtcaactgt atgaatctgt gtagtttgga aaggaaaaat
     8401 agggtgtttt gtacttgagt tttgtatttt aactacccat cattccagct ttttatatac
     8461 tatatttcat ttatgaagaa actggttttc tctgaggagt cacttcagtc tgtaatttta
     8521 aaaaagcaac agtcggaata tttatagttg attcttcact gtgcatacct atatatgccc
     8581 ttatcccttt aaatgcctaa gtagggcatg ctactaagaa tcaagctggt gaaaggcaga
     8641 tgtgcctatc tctgttggaa gccgactaac cctccgccct ccgtggactc gaagctggag
     8701 gtggactcag aaccctctgg gttgcagtca cacgcagacc cccttactct cccagtagtg
     8761 tgtgtggcag ttgcttggta gtagaaacct ggtggggcct gagccctgcc gttggcctct
     8821 gcctcttgtg gttcctgaga aggcagtaga acctggacaa ttgccacccc tctgttttac
     8881 tgaatccccc gtcagtgttc atcactgttg tgattttatc ccgacatgga aagcgctaag
     8941 aaatgaactt tccagttagc cacgcgctgc tgcaggtgtt tgataaccct gtttaaactg
     9001 gtgctgtgta catgggatgt actgatacag aacttctctt ctagggaaag ctaatcattt
     9061 gtcaatgatt taaaaattat tgtcactact gttgattact gtggactaag tgagcctcgt
     9121 gaaagagctg attggaattt gtacctttgt ggctgctcat gcattttcca tggtgctaca
     9181 gacagcctgc ccgggctggt ggctaggaaa cgtgcgtgtt cagcgatgcc tagcgctctc
     9241 actcctgagc actctgactt ggtgcccacc cctggcgcgt gctggtttgg cagtatgaaa
     9301 tgcccacgag ggttttgtat ctcacaaagg ttatgtagcc tgttctgtga ataatgaggg
     9361 aagcagtttc ttggtggaaa ttttaacatg cctttagctg tggcagtgat gggctttctt
     9421 tttccttctg tctgggtcag gttgtgcaca agtcatgtta tttggaaggt gcactgcact
     9481 gctgtttaca tgggcatttt atgcgggtgc tttgaagtgc cttgcatcag ggattagaat
     9541 caattcaatt ctttttcatg ggactatgca aagcatgtag tattgccttg gtatgtaact
     9601 attgtagttt taccatggag aaataccttt aaattataac tctggagagc agccatgagg
     9661 accctctgta cttcctggtg cttttcctta acatggtggg tgagtagcga gtgcggggca
     9721 ccctgtacat ggcagggtgg ggcgttctca ttaaataata ctgagatttt aaacatgcgc
     9781 agtctgagcg tttttacaat aaacattaag aatggctatg cttagtatac aggttttcct
     9841 ccagcaggat ttaatggtat tcctccccac tggaaacctg aggatggctt actttctctt
     9901 acatgtcaca tgacaaaaat cacaaaagct tgaatagttt atttttacag attctacatt
     9961 tttttaattt ataattttgt aaaagacaag actagcaacc tgtttttaag aaagaaaacc
    10021 ctgcagccag atgtggtggc ccaggccttc agttccagta cttaggaggc aaagacatga
    10081 ggatctttgt gagtttgaag ttgtgagttc caatttatcc agggatatag agaaagaccc
    10141 tgtttgtttg tttgtttgtt tttgaggcag cgtttctctc cgtagccttg gctgtcctga
    10201 aactcactgt atagaccagg ctggcctcga actcacagat taaactcttt ctgcctcctg
    10261 agtgctgaga ttaaaggtat gtgctgccac tacccggcta acttgtttct attattttga
    10321 aaacatgtat ggatattttg ccttccatat gtgcatcaca tgtgtaccca gtgcctgtga
    10381 aggccagaag agaatgtcag agcccctgga actgccatgt gggttctgag aatcaaagct
    10441 gggtcctctg gaagagcagc caacactctc tccagccagg gagatcccat cttagaagta
    10501 aaacaaaaga actgctccca aaatatctaa ctgagaaaat agagattttg aaaattttca
    10561 gatatagttg tcattcaggt tgagacttgg ggtacatagc gagaccctgt ctcaaaacac
    10621 caaaagaaaa gaatgagtag gggctgctca tggcaatcgt ttgaacttca agaagttaag
    10681 tatctgtgtg ggccttggtt caacttgtac acagtagtat ttgtctctta caccataggt
    10741 catatatatc agaagagttg ctcctaaaga acagtgttag aagatacaga gggcccgttc
    10801 catttttatc cagggtgtat tttaactgtt ggagtgtttc atctattgtg atgcagttta
    10861 aagatccctg cagggtcagg aacaaaccag ctttcccata agtcattgtg aacactagga
    10921 aagtcccagg gtttatgtct tccattccag tggagtagtt tagcttcctg caggaagtgc
    10981 tccgaatatc ttgcatcagg gttccagcct tcggtgtgtg tggagggaaa taaacaacag
    11041 gcttgttagt gacgacgatt ttatatggaa ccttggatac aagctatggt agaagtgtct
    11101 ggttagcagg agtcacgcct cacataagcc cccttgttga taaccataca ttacataagc
    11161 ctaaggttat gatagtgaag agatcttaat actgccattc ctcggagtgt tctacagtac
    11221 atctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga
    11281 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    11341 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    11401 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    11461 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatgtcgccg agctgggccc
    11521 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
    11581 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    11641 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    11701 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
    11761 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
    11821 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
    11881 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
    11941 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
    12001 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
    12061 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
    12121 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
    12181 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
    12241 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
    12301 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
    12361 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
    12421 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
    12481 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
    12541 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
    12601 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
    12661 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
    12721 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
    12781 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
    12841 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
    12901 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
    12961 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
    13021 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
    13081 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
    13141 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
    13201 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
    13261 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
    13321 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
    13381 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
    13441 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
    13501 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
    13561 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
    13621 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    13681 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
    13741 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
    13801 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
    13861 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
    13921 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
    13981 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
    14041 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
    14101 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
    14161 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
    14221 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
    14281 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
    14341 gcgcgtaata cgactcacta tagggcgaat tgggtacccg gtatacagac atgataagat
    14401 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
    14461 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttgggg
    14521 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg
    14581 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat
    14641 ggcaggttgg gcgtcgcttg gtcggtcatt tcgaactcga cgatgcatgg ggtcgtgcgc
    14701 tcctttcggt cgggcgctgc gggtcgtggg gcgggcgtca ggcaccgggc ttgcgggtca
    14761 tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg aagccgagcc
    14821 gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc accccggcgc
    14881 gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg ccctggtggt
    14941 cgggcgagac gccgacggtg gccaggaacc acgcgggctc cttgggccgg tgcggcgcca
    15001 ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg gccatgcgcg
    15061 ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc cagaccgcca
    15121 ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg cgcgtgagga
    15181 agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg gtgtggcgcg
    15241 tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg acgtcgtcgc
    15301 gggtggcgag gcgcaccgtg ggcttgtact cggtcatggt aagctttttg caaaagccta
    15361 ggcctccaaa aaagcctcct cactacttct ggaatagctc agaggccgag gcggcctcgg
    15421 cctctgcata aataaaaaaa attagtcagc catggggcgg agaatgggcg gaactgggcg
    15481 gagttagggg cgggatgggc ggagttaggg gcgggactat ggttgctgac taattgagat
    15541 gcatgctttg catacttctg cctgctgggg agcctgggga ctttccacac ctggttgctg
    15601 actaattgag atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac
    15661 accctaactg acacacattc cacaggg
//
