LOCUS       sequence-310063-       10893 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector expressing plant-codon-optimized Cas9 under UBQ10
            promoter (BamHI and NotI), with gRNAs be shuffled in; seed coat 
            specific red fluorescence for screening trangene free plants;.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10893)
  AUTHORS   Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, 
            Schwab R, Weigel D
  TITLE     An efficient CRISPR vector toolbox for engineering large deletions 
            in Arabidopsis thaliana.
  JOURNAL   Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. 
            eCollection 2018.
  PUBMED    30083222
REFERENCE   2  (bases 1 to 10893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2018-08-2"; volume: "14:65. doi"; pages: " 
            10.1186/s13007-018-0330-7. eCollection 2018"
COMMENT     Sequence Label: pRWJB004
FEATURES             Location/Qualifiers
     source          1..10893
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             1238..1285
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             1292..1312
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1337..2773,2963..5626)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          2774..2962
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             5627..5674
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5680..6321
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(6544..6867)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     6777..6793
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6863..6879)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6863..6879)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6876..6898)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6887..6903
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6911..6917,6918..6935,6936..6941))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6956..6977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7094..7111)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7490..7742)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(8026..8045)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8295..8313
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    8912..8936
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9027..9615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9107..9126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9786..10574)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(10865..10893,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgccgacgag
      601 tcagtaataa acggcgtcaa agtggttgca gccggcacac acgagtcgtg tttatcaact
      661 caaagcacaa atacttttcc tcaacctaaa aataaggcaa ttagccaaaa acaactttgc
      721 gtgtaaacaa cgctcaatac acgtgtcatt ttattattag ctattgcttc accgccttag
      781 ctttctcgtg acctagtcgt cctcgtcttt tcttcttctt cttctataaa acaataccca
      841 aagagctctt cttcttcaca attcagattt caatttctca aaatcttaaa aactttctct
      901 caattctctc taccgtgatc aaggtaaatt tctgtgttcc ttattctctc aaaatcttcg
      961 attttgtttt cgttcgatcc caatttcgta tatgttcttt ggtttagatt ctgttaatct
     1021 tagatcgaag acgattttct gggtttgatc gttagatatc atcttaattc tcgattaggg
     1081 tttcatagat atcatccgat ttgttcaaat aatttgagtt ttgtcgaata attactcttc
     1141 gatttgtgat ttctatctag atctggtgtt agtttctagt ttgtgcgatc gaatttgtag
     1201 attaatctga gtttttctga ttaacagcgg atccatggat tacaaggatg atgatgataa
     1261 ggattacaag gatgatgatg ataagatggc tccaaagaag aagagaaagg ttggaatcca
     1321 cggagttcca gctgctgata agaagtactc tatcggactt gacatcggaa ccaactctgt
     1381 tggatgggct gttatcaccg atgagtacaa ggttccatct aagaagttca aggttcttgg
     1441 aaacaccgat agacactcta tcaagaagaa ccttatcggt gctcttcttt tcgattctgg
     1501 agaaaccgct gaggctacca gattgaagag aaccgctaga agaagataca ccagaagaaa
     1561 gaacagaatc tgctaccttc aggaaatctt ctctaacgag atggctaagg ttgatgattc
     1621 tttcttccac agacttgagg agtctttcct tgttgaggag gataagaagc acgagagaca
     1681 cccaatcttc ggaaacatcg ttgatgaggt tgcttaccac gagaagtacc caaccatcta
     1741 ccaccttaga aagaagttgg ttgattctac cgataaggct gatcttagac ttatctacct
     1801 tgctcttgct cacatgatca agttcagagg acacttcctt atcgagggtg accttaaccc
     1861 agataactct gatgttgata agttgttcat ccagcttgtt cagacctaca accagctttt
     1921 cgaggagaac ccaatcaacg cttctggagt tgatgctaag gctatccttt ctgctagact
     1981 ttctaagtct cgtagacttg agaaccttat cgctcagctt ccaggagaga agaagaacgg
     2041 acttttcgga aaccttatcg ctctttctct tggacttacc ccaaacttca agtctaactt
     2101 cgatcttgct gaggatgcta agttgcagct ttctaaggat acctacgatg atgatcttga
     2161 taaccttctt gctcagatcg gagatcagta cgctgatctt ttccttgctg ctaagaacct
     2221 ttctgatgct atccttcttt ctgacatcct tagagttaac accgagatca ccaaggctcc
     2281 actttctgct tctatgatca agagatacga tgagcaccac caggatctta cccttttgaa
     2341 ggctcttgtt agacagcagc ttccagagaa gtacaaggaa atcttcttcg atcagtctaa
     2401 gaacggatac gctggataca tcgatggagg agcttctcag gaggagttct acaagttcat
     2461 caagccaatc cttgagaaga tggatggaac cgaggagctt cttgttaagt tgaacagaga
     2521 ggatcttctt agaaagcaga gaaccttcga taacggatct atcccacacc agatccacct
     2581 tggagagctt cacgctatcc ttcgtagaca ggaggatttc tacccattct tgaaggataa
     2641 cagagagaag atcgagaaga tccttacctt cagaatccca tactacgttg gaccacttgc
     2701 tagaggaaac tctcgtttcg cttggatgac cagaaagtct gaggaaacca tcaccccttg
     2761 gaacttcgag gaggtaagtt tctgcttcta cctttgatat atatataata attatcatta
     2821 attagtagta atataatatt tcaaatattt ttttcaaaat aaaagaatgt agtatatagc
     2881 aattgctttt ctgtagttta taagtgtgta tattttaatt tataactttt ctaatatatg
     2941 accaaaattt gttgatgtgc aggttgttga taagggagct tctgctcagt ctttcatcga
     3001 gagaatgacc aacttcgata agaaccttcc aaacgagaag gttcttccaa agcactctct
     3061 tctttacgag tacttcaccg tttacaacga gcttaccaag gttaagtacg ttaccgaggg
     3121 aatgagaaag ccagctttcc tttctggaga gcagaagaag gctatcgttg atcttctttt
     3181 caagaccaac agaaaggtta ccgttaagca gttgaaggag gattacttca agaagatcga
     3241 gtgcttcgat tctgttgaaa tctctggagt tgaggataga ttcaacgctt ctcttggaac
     3301 ctaccacgat cttttgaaga tcatcaagga taaggatttc cttgataacg aggagaacga
     3361 ggacatcctt gaggacatcg ttcttaccct tacccttttc gaggatagag agatgatcga
     3421 ggagagactc aagacctacg ctcacctttt cgatgataag gttatgaagc agttgaagag
     3481 aagaagatac accggatggg gtagactttc tcgtaagttg atcaacggaa tcagagataa
     3541 gcagtctgga aagaccatcc ttgatttctt gaagtctgat ggattcgcta acagaaactt
     3601 catgcagctt atccacgatg attctcttac cttcaaggag gacatccaga aggctcaggt
     3661 ttctggacag ggagattctc ttcacgagca catcgctaac cttgctggat ctccagctat
     3721 caagaaggga atccttcaga ccgttaaggt tgttgatgag cttgttaagg ttatgggtag
     3781 acacaagcca gagaacatcg ttatcgagat ggctagagag aaccagacca cccagaaggg
     3841 acagaagaac tctcgtgaga gaatgaagag aatcgaggag ggaatcaagg agcttggatc
     3901 tcaaatcttg aaggagcacc cagttgagaa cacccagctt cagaacgaga agttgtacct
     3961 ttactacctt cagaacggaa gagatatgta cgttgatcag gagcttgaca tcaacagact
     4021 ttctgattac gatgttgatc acatcgttcc acagtctttc ttgaaggatg attctatcga
     4081 taacaaggtt cttacccgtt ctgataagaa cagaggaaag tctgataacg ttccatctga
     4141 ggaggttgtt aagaagatga agaactactg gagacagctt cttaacgcta agttgatcac
     4201 ccagagaaag ttcgataacc ttaccaaggc tgagagagga ggactttctg agcttgataa
     4261 ggctggattc atcaagagac agcttgttga aaccagacag atcaccaagc acgttgctca
     4321 gatccttgat tctcgtatga acaccaagta cgatgagaac gataagttga tcagagaggt
     4381 taaggttatc accttgaagt ctaagttggt ttctgatttc agaaaggatt tccagttcta
     4441 caaggttaga gagatcaaca actaccacca cgctcacgat gcttacctta acgctgttgt
     4501 tggaaccgct cttatcaaga agtacccaaa gttggagtct gagttcgttt acggagatta
     4561 caaggtttac gatgttagaa agatgatcgc taagtctgag caggagatcg gaaaggctac
     4621 cgctaagtac ttcttctact ctaacatcat gaacttcttc aagaccgaga tcacccttgc
     4681 taacggagag atcagaaaga ggccacttat cgaaaccaac ggagaaaccg gagagatcgt
     4741 ttgggataag ggaagagatt tcgctaccgt tagaaaggtt ctttctatgc cacaggttaa
     4801 catcgttaag aaaaccgagg ttcagaccgg aggattctct aaggagtcta tccttccaaa
     4861 gagaaactct gataagttga tcgctagaaa gaaggattgg gacccaaaga agtacggagg
     4921 attcgattct ccaaccgttg cttactctgt tcttgttgtt gctaaggttg agaagggaaa
     4981 gtctaagaag ttgaagtctg ttaaggagct tcttggaatc accatcatgg agcgttcttc
     5041 tttcgagaag aacccaatcg atttccttga ggctaaggga tacaaggagg ttaagaagga
     5101 tcttatcatc aagttgccaa agtactctct tttcgagctt gagaacggaa gaaagagaat
     5161 gcttgcttct gctggagagc ttcagaaggg aaacgagctt gctcttccat ctaagtacgt
     5221 taacttcctt taccttgctt ctcactacga gaagttgaag ggatctccag aggataacga
     5281 gcagaagcag cttttcgttg agcagcacaa gcactacctt gatgagatca tcgagcaaat
     5341 ctctgagttc tctaagagag ttatccttgc tgatgctaac cttgataagg ttctttctgc
     5401 ttacaacaag cacagagata agccaatcag agagcaggct gagaacatca tccacctttt
     5461 cacccttacc aaccttggtg ctccagctgc tttcaagtac ttcgatacca ccatcgatag
     5521 aaaaagatac acctctacca aggaggttct tgatgctacc cttatccacc agtctatcac
     5581 cggactttac gagaccagaa tcgatctttc tcagcttgga ggagataaga gaccagctgc
     5641 taccaagaag gctggacagg ctaagaagaa gaagtgaaaa gctttcgttc gtatcatcgg
     5701 tttcgacaac gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac cagaatccta
     5761 ctgagtttga gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg
     5821 taatttactg tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga
     5881 gaagagttaa tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt
     5941 tctcttattt gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg
     6001 agtaaaaatg tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac
     6061 ttgtagttgt accattatgc ttattcacta ggcaacaaat atattttcag acctagaaaa
     6121 gctgcaaatg ttactgaata caagtatgtc ctcttgtgtt ttagacattt atgaactttc
     6181 ctttatgtaa ttttccagaa tccttgtcag attctaatca ttgctttata attatagtta
     6241 tactcatgga tttgtagttg agtatgaaaa tattttttaa tgcattttat gacttgccaa
     6301 ttgattgaca acatgcatca atcggtacca cctagtagaa gagctcagtg agaccgaatt
     6361 cgcttactaa aagccagata acagtatgcg tatttgcgcg ctgatttttg cggtataaga
     6421 atatatactg atatgtatac ccgaagtatg tcaaaaagag gtgtgctaag cttcggatgc
     6481 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     6541 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     6601 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     6661 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     6721 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     6781 aacgacggcc agtgaattcg agctcggtac acagggatct tcaagagtcg acctgcaggc
     6841 atgcaggctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6901 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6961 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     7021 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7081 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7141 tatcactagt aggaggtaat caatgctggc cgtcgtttta caacgtcgtg actgggaaaa
     7201 ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa
     7261 tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg
     7321 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatggtg
     7381 cactctcagt acaatctgct ctgatgccgc atgatcatct ggcggacctc ttcggaggtc
     7441 cgcttttttt tctcgagggt ctcaactagc tcttcatggg tattctagag ataatttatt
     7501 tgaaaattca taagaaaagc aaacgttaca tgaattgatg aaacaataca aagacagata
     7561 aagccacgca catttaggat attggccgag attactgaat attgagtaag atcacggaat
     7621 ttctgacagg agcatgtctt caattcagcc caaatggcag ttgaaatact caaaccgccc
     7681 catatgcagg agcggatcat tcattgtttg tttggttgcc tttgccaaca tgggagtcca
     7741 agagctctca cttgtacagc tcgtccatgc cgccggtgga gtggcggccc tcggcgcgtt
     7801 cgtactgttc cacgatggtg tagtcctcgt tgtgggaggt gatgtccaac ttgatgttga
     7861 cgttgtaggc gccgggcagc tgcacgggct tcttggcctt gtaggtggtc ttgacctcag
     7921 cgtcgtagtg gccgccgtcc ttcagcttca gcctctgctt gatctcgccc ttcagggcgc
     7981 cgtcctcggg gtacatccgc tcggaggagg cctcccagcc catggtcttc ttctgcatta
     8041 cggggccgtc ggaggggaag ttggtgccgc gcagcttcac cttgtagatg aactcgccgt
     8101 cctgcaggga ggagtcctgg gtcacggtca ccacgccgcc gtcctcgaag ttcatcacgc
     8161 gctcccactt gaagccctcg gggaaggaca gcttcaagta gtcggggatg tcggcggggt
     8221 gcttcacgta ggccttggag ccgtacatga actgagggga caggatgtcc caggcgaagg
     8281 gcagggggcc acccttggtc accttcagct tggcggtctg ggtgccctcg taggggcggc
     8341 cctcgccctc gccctcgatc tcgaactcgt ggccgttcac ggagccctcc atgtgcacct
     8401 tgaagcgcat gaactccttg atgatggcca tgttatccat tgttcgtacc gttttgctat
     8461 ttgtgtatgt tttcttgttt tggtttgagt gaagagtgta gtgaagggtt ggtttgtatt
     8521 tataggtgag aaaggagatt tgcatggcaa tcacgtgtaa gaatgcatgc atgcatgagt
     8581 gtgtgtggag agagagagat gctcttacgt tgttgtcggg attatgtgtt ggaaaagagt
     8641 gacaagtgac gaacaaaaca ataccactca tcatagctcc gcttatgcat gctatgtgtg
     8701 atttgtagct cttgttctga catgacacta tgaacaattt tttaaaaaga aggttaaact
     8761 tttatttaaa taaaaaccct atgttaattt ggtttcaatc gagtaagatg agaagtcaat
     8821 cttttgatgg tagctagtga tgttggaaat atagttgtgc cagcttatct agaccaagca
     8881 tgcacagctg gcgttctcag ccagatcccc ttgacaggat atattggcgg gtaaactaag
     8941 tcgctgtatg tgtttgtttg agatctcatg tgagcaaaag gccagcaaaa ggccaggaac
     9001 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     9061 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     9121 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     9181 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     9241 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     9301 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     9361 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     9421 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     9481 atctgcgctc tgctgaagcc agttaccttc ggaagaagag ttggtagctc ttgatccggc
     9541 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     9601 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     9661 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     9721 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgtgta acattggtct
     9781 agtgattatt tgccgactac cttggtgatc tcgcctttca cgtagtgaac aaattcttcc
     9841 aactgatctg cgcgcgaggc caagcgatct tcttgtccaa gataagcctg cctagcttca
     9901 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     9961 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
    10021 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
    10081 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
    10141 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
    10201 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
    10261 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
    10321 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
    10381 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
    10441 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
    10501 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
    10561 accgcttccc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
    10621 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
    10681 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
    10741 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
    10801 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcg
    10861 atccccatcc aacagcccgc cgtcgagcgg gct
//
