LOCUS       sequence-310065-       11121 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector expressing plant-codon-optimized Cas9 under 35S 
            promoter (BamHI and NotI), with sgRNAs be shuffled in; seed coat 
            specific red fluorescence for screening trangene free.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11121)
  AUTHORS   Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, 
            Schwab R, Weigel D
  TITLE     An efficient CRISPR vector toolbox for engineering large deletions 
            in Arabidopsis thaliana.
  JOURNAL   Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. 
            eCollection 2018.
  PUBMED    30083222
REFERENCE   2  (bases 1 to 11121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods."; date: "2018-08-2"; volume: "14:65. doi"; pages: " 
            10.1186/s13007-018-0330-7. eCollection 2018"
COMMENT     Sequence Label: pRWJB006
FEATURES             Location/Qualifiers
     source          1..11121
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     promoter        1092..1437
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     1380..1400
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1466..1513
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     CDS             1520..1540
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1565..3001,3191..5854)
                     /codon_start=1
                     /product="Cas9 endonuclease from the Streptococcus pyogenes
                     Type II CRISPR/Cas system"
                     /label=pcoCas9
                     /note="plant codon-optimized Cas9 gene containing the 
                     potato IV2 intron"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     intron          3002..3190
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
     CDS             5855..5902
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     terminator      5908..6549
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     CDS             complement(6772..7095)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPCVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     primer_bind     7005..7021
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7091..7107)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7091..7107)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7104..7126)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7115..7131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7139..7145,7146..7163,7164..7169))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7184..7205
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7322..7339)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(7718..7970)
                     /label=MAS terminator
                     /note="mannopine synthase terminator"
     primer_bind     complement(8254..8273)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     8523..8541
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     misc_feature    9140..9164
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(9255..9843)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9335..9354)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10014..10802)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(11093..11121,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcattcggt
      601 ccccagatta gccttttcaa tttcagaaag aatgctaacc cacagatggt tagagaggct
      661 tacgcagcac gtctcatcaa gacgatctac ccgagcaata atctccagga aatcaaatac
      721 cttcccaaga aggttaaaga tgcagtcaaa agattcagga ctaactgcat caagaacaca
      781 gagaaagata tatttctcaa gatcagaagt actattccag tatggacgat tcaaggcttg
      841 cttcacaaac caaggcaagt aatagagatt ggagtctcta aaaaggtagt tcccactgaa
      901 tcaaaggcca tggagtcaaa gattcaaata gaggacctaa cagaactcgc cgtaaagact
      961 ggcgaacagt tcatacagag tctcttacga ctcaatgaca agaagaaaat cttcgtcaac
     1021 atggtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac
     1081 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat
     1141 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa
     1201 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc
     1261 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct
     1321 tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac
     1381 tatccttcgc aagacccttc ctctatataa ggaagttcat ttcatttgga gagaacacgg
     1441 gggacgagct aacagcggat ccatggatta caaggatgat gatgataagg attacaagga
     1501 tgatgatgat aagatggctc caaagaagaa gagaaaggtt ggaatccacg gagttccagc
     1561 tgctgataag aagtactcta tcggacttga catcggaacc aactctgttg gatgggctgt
     1621 tatcaccgat gagtacaagg ttccatctaa gaagttcaag gttcttggaa acaccgatag
     1681 acactctatc aagaagaacc ttatcggtgc tcttcttttc gattctggag aaaccgctga
     1741 ggctaccaga ttgaagagaa ccgctagaag aagatacacc agaagaaaga acagaatctg
     1801 ctaccttcag gaaatcttct ctaacgagat ggctaaggtt gatgattctt tcttccacag
     1861 acttgaggag tctttccttg ttgaggagga taagaagcac gagagacacc caatcttcgg
     1921 aaacatcgtt gatgaggttg cttaccacga gaagtaccca accatctacc accttagaaa
     1981 gaagttggtt gattctaccg ataaggctga tcttagactt atctaccttg ctcttgctca
     2041 catgatcaag ttcagaggac acttccttat cgagggtgac cttaacccag ataactctga
     2101 tgttgataag ttgttcatcc agcttgttca gacctacaac cagcttttcg aggagaaccc
     2161 aatcaacgct tctggagttg atgctaaggc tatcctttct gctagacttt ctaagtctcg
     2221 tagacttgag aaccttatcg ctcagcttcc aggagagaag aagaacggac ttttcggaaa
     2281 ccttatcgct ctttctcttg gacttacccc aaacttcaag tctaacttcg atcttgctga
     2341 ggatgctaag ttgcagcttt ctaaggatac ctacgatgat gatcttgata accttcttgc
     2401 tcagatcgga gatcagtacg ctgatctttt ccttgctgct aagaaccttt ctgatgctat
     2461 ccttctttct gacatcctta gagttaacac cgagatcacc aaggctccac tttctgcttc
     2521 tatgatcaag agatacgatg agcaccacca ggatcttacc cttttgaagg ctcttgttag
     2581 acagcagctt ccagagaagt acaaggaaat cttcttcgat cagtctaaga acggatacgc
     2641 tggatacatc gatggaggag cttctcagga ggagttctac aagttcatca agccaatcct
     2701 tgagaagatg gatggaaccg aggagcttct tgttaagttg aacagagagg atcttcttag
     2761 aaagcagaga accttcgata acggatctat cccacaccag atccaccttg gagagcttca
     2821 cgctatcctt cgtagacagg aggatttcta cccattcttg aaggataaca gagagaagat
     2881 cgagaagatc cttaccttca gaatcccata ctacgttgga ccacttgcta gaggaaactc
     2941 tcgtttcgct tggatgacca gaaagtctga ggaaaccatc accccttgga acttcgagga
     3001 ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat
     3061 ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct
     3121 gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt
     3181 tgatgtgcag gttgttgata agggagcttc tgctcagtct ttcatcgaga gaatgaccaa
     3241 cttcgataag aaccttccaa acgagaaggt tcttccaaag cactctcttc tttacgagta
     3301 cttcaccgtt tacaacgagc ttaccaaggt taagtacgtt accgagggaa tgagaaagcc
     3361 agctttcctt tctggagagc agaagaaggc tatcgttgat cttcttttca agaccaacag
     3421 aaaggttacc gttaagcagt tgaaggagga ttacttcaag aagatcgagt gcttcgattc
     3481 tgttgaaatc tctggagttg aggatagatt caacgcttct cttggaacct accacgatct
     3541 tttgaagatc atcaaggata aggatttcct tgataacgag gagaacgagg acatccttga
     3601 ggacatcgtt cttaccctta cccttttcga ggatagagag atgatcgagg agagactcaa
     3661 gacctacgct caccttttcg atgataaggt tatgaagcag ttgaagagaa gaagatacac
     3721 cggatggggt agactttctc gtaagttgat caacggaatc agagataagc agtctggaaa
     3781 gaccatcctt gatttcttga agtctgatgg attcgctaac agaaacttca tgcagcttat
     3841 ccacgatgat tctcttacct tcaaggagga catccagaag gctcaggttt ctggacaggg
     3901 agattctctt cacgagcaca tcgctaacct tgctggatct ccagctatca agaagggaat
     3961 ccttcagacc gttaaggttg ttgatgagct tgttaaggtt atgggtagac acaagccaga
     4021 gaacatcgtt atcgagatgg ctagagagaa ccagaccacc cagaagggac agaagaactc
     4081 tcgtgagaga atgaagagaa tcgaggaggg aatcaaggag cttggatctc aaatcttgaa
     4141 ggagcaccca gttgagaaca cccagcttca gaacgagaag ttgtaccttt actaccttca
     4201 gaacggaaga gatatgtacg ttgatcagga gcttgacatc aacagacttt ctgattacga
     4261 tgttgatcac atcgttccac agtctttctt gaaggatgat tctatcgata acaaggttct
     4321 tacccgttct gataagaaca gaggaaagtc tgataacgtt ccatctgagg aggttgttaa
     4381 gaagatgaag aactactgga gacagcttct taacgctaag ttgatcaccc agagaaagtt
     4441 cgataacctt accaaggctg agagaggagg actttctgag cttgataagg ctggattcat
     4501 caagagacag cttgttgaaa ccagacagat caccaagcac gttgctcaga tccttgattc
     4561 tcgtatgaac accaagtacg atgagaacga taagttgatc agagaggtta aggttatcac
     4621 cttgaagtct aagttggttt ctgatttcag aaaggatttc cagttctaca aggttagaga
     4681 gatcaacaac taccaccacg ctcacgatgc ttaccttaac gctgttgttg gaaccgctct
     4741 tatcaagaag tacccaaagt tggagtctga gttcgtttac ggagattaca aggtttacga
     4801 tgttagaaag atgatcgcta agtctgagca ggagatcgga aaggctaccg ctaagtactt
     4861 cttctactct aacatcatga acttcttcaa gaccgagatc acccttgcta acggagagat
     4921 cagaaagagg ccacttatcg aaaccaacgg agaaaccgga gagatcgttt gggataaggg
     4981 aagagatttc gctaccgtta gaaaggttct ttctatgcca caggttaaca tcgttaagaa
     5041 aaccgaggtt cagaccggag gattctctaa ggagtctatc cttccaaaga gaaactctga
     5101 taagttgatc gctagaaaga aggattggga cccaaagaag tacggaggat tcgattctcc
     5161 aaccgttgct tactctgttc ttgttgttgc taaggttgag aagggaaagt ctaagaagtt
     5221 gaagtctgtt aaggagcttc ttggaatcac catcatggag cgttcttctt tcgagaagaa
     5281 cccaatcgat ttccttgagg ctaagggata caaggaggtt aagaaggatc ttatcatcaa
     5341 gttgccaaag tactctcttt tcgagcttga gaacggaaga aagagaatgc ttgcttctgc
     5401 tggagagctt cagaagggaa acgagcttgc tcttccatct aagtacgtta acttccttta
     5461 ccttgcttct cactacgaga agttgaaggg atctccagag gataacgagc agaagcagct
     5521 tttcgttgag cagcacaagc actaccttga tgagatcatc gagcaaatct ctgagttctc
     5581 taagagagtt atccttgctg atgctaacct tgataaggtt ctttctgctt acaacaagca
     5641 cagagataag ccaatcagag agcaggctga gaacatcatc caccttttca cccttaccaa
     5701 ccttggtgct ccagctgctt tcaagtactt cgataccacc atcgatagaa aaagatacac
     5761 ctctaccaag gaggttcttg atgctaccct tatccaccag tctatcaccg gactttacga
     5821 gaccagaatc gatctttctc agcttggagg agataagaga ccagctgcta ccaagaaggc
     5881 tggacaggct aagaagaaga agtgaaaagc tttcgttcgt atcatcggtt tcgacaacgt
     5941 tcgtcaagtt caatgcatca gtttcattgc gcacacacca gaatcctact gagtttgagt
     6001 attatggcat tgggaaaact gtttttcttg taccatttgt tgtgcttgta atttactgtg
     6061 ttttttattc ggttttcgct atcgaactgt gaaatggaaa tggatggaga agagttaatg
     6121 aatgatatgg tccttttgtt cattctcaaa ttaatattat ttgttttttc tcttatttgt
     6181 tgtgtgttga atttgaaatt ataagagata tgcaaacatt ttgttttgag taaaaatgtg
     6241 tcaaatcgtg gcctctaatg accgaagtta atatgaggag taaaacactt gtagttgtac
     6301 cattatgctt attcactagg caacaaatat attttcagac ctagaaaagc tgcaaatgtt
     6361 actgaataca agtatgtcct cttgtgtttt agacatttat gaactttcct ttatgtaatt
     6421 ttccagaatc cttgtcagat tctaatcatt gctttataat tatagttata ctcatggatt
     6481 tgtagttgag tatgaaaata ttttttaatg cattttatga cttgccaatt gattgacaac
     6541 atgcatcaat cggtaccacc tagtagaaga gctcagtgag accgaattcg cttactaaaa
     6601 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     6661 atgtataccc gaagtatgtc aaaaagaggt gtgctaagct tcggatgccg ggagcagaca
     6721 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     6781 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     6841 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     6901 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     6961 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     7021 tgaattcgag ctcggtacac agggatcttc aagagtcgac ctgcaggcat gcaggcttgg
     7081 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     7141 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     7201 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     7261 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     7321 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcactagtag
     7381 gaggtaatca atgctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     7441 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     7501 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     7561 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     7621 aatctgctct gatgccgcat gatcatctgg cggacctctt cggaggtccg cttttttttc
     7681 tcgagggtct caactagctc ttcatgggta ttctagagat aatttatttg aaaattcata
     7741 agaaaagcaa acgttacatg aattgatgaa acaatacaaa gacagataaa gccacgcaca
     7801 tttaggatat tggccgagat tactgaatat tgagtaagat cacggaattt ctgacaggag
     7861 catgtcttca attcagccca aatggcagtt gaaatactca aaccgcccca tatgcaggag
     7921 cggatcattc attgtttgtt tggttgcctt tgccaacatg ggagtccaag agctctcact
     7981 tgtacagctc gtccatgccg ccggtggagt ggcggccctc ggcgcgttcg tactgttcca
     8041 cgatggtgta gtcctcgttg tgggaggtga tgtccaactt gatgttgacg ttgtaggcgc
     8101 cgggcagctg cacgggcttc ttggccttgt aggtggtctt gacctcagcg tcgtagtggc
     8161 cgccgtcctt cagcttcagc ctctgcttga tctcgccctt cagggcgccg tcctcggggt
     8221 acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg gggccgtcgg
     8281 aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa ctcgccgtcc tgcagggagg
     8341 agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc tcccacttga
     8401 agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc ttcacgtagg
     8461 ccttggagcc gtacatgaac tgaggggaca ggatgtccca ggcgaagggc agggggccac
     8521 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
     8581 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
     8641 actccttgat gatggccatg ttatccattg ttcgtaccgt tttgctattt gtgtatgttt
     8701 tcttgttttg gtttgagtga agagtgtagt gaagggttgg tttgtattta taggtgagaa
     8761 aggagatttg catggcaatc acgtgtaaga atgcatgcat gcatgagtgt gtgtggagag
     8821 agagagatgc tcttacgttg ttgtcgggat tatgtgttgg aaaagagtga caagtgacga
     8881 acaaaacaat accactcatc atagctccgc ttatgcatgc tatgtgtgat ttgtagctct
     8941 tgttctgaca tgacactatg aacaattttt taaaaagaag gttaaacttt tatttaaata
     9001 aaaaccctat gttaatttgg tttcaatcga gtaagatgag aagtcaatct tttgatggta
     9061 gctagtgatg ttggaaatat agttgtgcca gcttatctag accaagcatg cacagctggc
     9121 gttctcagcc agatcccctt gacaggatat attggcgggt aaactaagtc gctgtatgtg
     9181 tttgtttgag atctcatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9241 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9301 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9361 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9421 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     9481 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9541 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9601 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9661 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     9721 ctgaagccag ttaccttcgg aagaagagtt ggtagctctt gatccggcaa acaaaccacc
     9781 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9841 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     9901 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     9961 aaatgaagtt ttaaatcaat ctaaagtata tatgtgtaac attggtctag tgattatttg
    10021 ccgactacct tggtgatctc gcctttcacg tagtgaacaa attcttccaa ctgatctgcg
    10081 cgcgaggcca agcgatcttc ttgtccaaga taagcctgcc tagcttcaag tatgacgggc
    10141 tgatactggg ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt
    10201 ttgccggtta ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg
    10261 ccagcccagt cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga
    10321 tcctgttcag gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta
    10381 tgttctcttg cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag
    10441 atacctgcaa gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga
    10501 taacgccacg gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc
    10561 tcgctctctc caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt
    10621 gtttcatcaa gccttacggt caccgtaacc agcaaatcaa tatcactgtg tggcttcagg
    10681 ccgccatcca ctgcggagcc gtacaaatgt acggccagca acgtcggttc gagatggcgc
    10741 tcgatgacgc caactacctc tgatagttga gtcgatactt cggcgatcac cgcttccctc
    10801 ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca tgatgatata
    10861 tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggct ttgttgaata
    10921 aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa cgcagaccgt
    10981 tccgtggcaa agcaaaagtt caaaatcacc aactggtcca cctacaacaa agctctcatc
    11041 aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat ccccatccaa
    11101 cagcccgccg tcgagcgggc t
//
