LOCUS       sequence-31007-e         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Tabor JJ, Salis HM, Simpson ZB, Chevalier AA, Levskaya A, Marcotte 
            EM, Voigt CA, Ellington AD
  TITLE     A synthetic genetic edge detection program.
  JOURNAL   Cell. 2009 Jun 26. 137(7):1272-81.
  PUBMED    19563759
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2009 
            Jun 26. 137(7):1272-81."
COMMENT     Sequence Label: pEDL3
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(188..292)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     357..375
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(379..422)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    425..446
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     RBS             552..563
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     primer_bind     complement(586..608)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
       61 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      121 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      181 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      241 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      301 ccatggtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
      361 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
      421 tctggaattc gcggccgctt ctagagtaag cacctgtagg atcgtacagg tttacgcaag
      481 aaaatggttt gttatagtcg aataacaccg tgcgtgttga ctattttacc tctggcggtg
      541 atatactgga gaaagaggag aaatactaga gatgatagat cccgtcgttt tacaacgtcg
      601 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      661 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      721 gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg aaagctggct
      781 ggagtgcgat cttcctga
//
