LOCUS       sequence-310076-        9222 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tamoxifen-inducible Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9222)
  AUTHORS   Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB,
            Schar P, Schuermann D
  TITLE     Inducible TDG knockout models to study epigenetic regulation.
  JOURNAL   F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. 
            eCollection 2020.
  PUBMED    33082936
REFERENCE   2  (bases 1 to 9222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2020-09-9"; volume: "9:1112. doi"; pages: " 
            10.12688/f1000research.25637.2. eCollection 2020"
COMMENT     Sequence Label: pBigTB-ERT2CreERT2
FEATURES             Location/Qualifiers
     source          1..9222
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..398
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        400..675
                     /label=chicken beta-actin promoter
     intron          676..1692
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1616..1638
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1700..1719
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1758..1767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1767..2702
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2712..3734
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             3747..4682
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3795..4688
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(4714..4731)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4720..4944
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(5016..5034)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5055..5071)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5055..5071)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5068..5090)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5079..5095
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5103..5109,5110..5127,5128..5133))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5148..5169
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5286..5303)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5457..6045)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5537..5556)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6216..7007,7008..7076))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6839..6858
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7077..7181)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7234..7662)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7344..7365)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7556..7575
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7789..7811
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7803..7820
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7804..7820
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7827..7846
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7827..7845
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(7873..7938,7939..7976)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(8089..8109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8094..8423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8274..8409
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8336..8355
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8471..8518
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8537..8935
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    9093..9214
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9130..9149)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9184..9203
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gctagcatcg ataccgtcga cattgattat tgactagtta ttaatagtaa tcaattacgg
       61 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      121 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      181 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      241 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      301 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      361 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc cccacgttct
      421 gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt
      481 aattattttg tgcagcgatg ggggcggggg gggggggggg gcgcgccagg cggggcgggg
      541 cggggcgagg ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc
      601 gcgctccgaa agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga
      661 agcgcgcggc gggcgggagt cgctgcgcgc tgccttcgcc ccgtgccccg ctccgccgcc
      721 gcctcgcgcc gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg
      781 gacggccctt ctcctccggg ctgtaattag cgcttggttt aatgacggct tgtttctttt
      841 ctgtggctgc gtgaaagcct tgaggggctc cgggagggcc ctttgtgcgg ggggagcggc
      901 tcggggggtg cgtgcgtgtg tgtgtgcgtg gggagcgccg cgtgcggctc cgcgctgccc
      961 ggcggctgtg agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcag tgtgcgcgag
     1021 gggagcgcgg ccgggggcgg tgccccgcgg tgcggggggg gctgcgaggg gaacaaaggc
     1081 tgcgtgcggg gtgtgtgcgt gggggggtga gcagggggtg tgggcgcgtc ggtcgggctg
     1141 caaccccccc tgcacccccc tccccgagtt gctgagcacg gcccggcttc gggtgcgggg
     1201 ctccgtacgg ggcgtggcgc ggggctcgcc gtgccgggcg gggggtggcg gcaggtgggg
     1261 gtgccgggcg gggcggggcc gcctcgggcc ggggagggct cgggggaggg gcgcggcggc
     1321 ccccggagcg ccggcggctg tcgaggcgcg gcgagccgca gccattgcct tttatggtaa
     1381 tcgtgcgaga gggcgcaggg acttcctttg tcccaaatct gtgcggagcc gaaatctggg
     1441 aggcgccgcc gcaccccctc tagcgggcgc ggggcgaagc ggtgcggcgc cggcaggaag
     1501 gaaatgggcg gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccctctccag
     1561 cctcggggct gtccgcgggg ggacggctgc cttcgggggg gacggggcag ggcggggttc
     1621 ggcttctggc gtgtgaccgg cggctctaga gcctctgcta accatgttca tgccttcttc
     1681 tttttcctac agctcctggg caacgtgctg gttattgtgc tgtctcatca ttttggcaaa
     1741 gaattcccgg gtgagccgcc accatggctg gagacatgag agctgccaac ctttggccaa
     1801 gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg acggccgacc
     1861 agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag tatgatccta
     1921 ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca gacagggagc
     1981 tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg accctccatg
     2041 atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt ctcgtctggc
     2101 gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg gacaggaacc
     2161 agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct acatcatctc
     2221 ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct attattttgc
     2281 ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa gagaaggacc
     2341 atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg gccaaggcag
     2401 gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc ctctcccaca
     2461 tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc aagaacgtgg
     2521 tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat gcgcccacta
     2581 gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact gcgggctcta
     2641 cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt ttccctgcca
     2701 cagctgtcga caatttactg accgtacacc aaaatttgcc tgcattaccg gtcgatgcaa
     2761 cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag gcgttttctg
     2821 agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg tgcaagttga
     2881 ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt ctatatcttc
     2941 aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta aacatgcttc
     3001 atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg gttatgcggc
     3061 ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta gcgttcgaac
     3121 gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc caggatatac
     3181 gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc gaaattgcca
     3241 ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc catattggca
     3301 gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg ggggtaacta
     3361 aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat aactacctgt
     3421 tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag ctatcaactc
     3481 gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct aaggatgact
     3541 ctggtcagag atacctggcc tggtctggac acagtgcccg tgtcggagcc gcgcgagata
     3601 tggcccgcgc tggagtttca ataccggaga tcatgcaagc tggtggctgg accaatgtaa
     3661 atattgtcat gaactatatc cgtaacctgg atagtgaaac aggggcaatg gtgcgcctgc
     3721 tggaagatgg cgatctcgag ccatctgctg gagacatgag agctgccaac ctttggccaa
     3781 gcccgctcat gatcaaacgc tctaagaaga acagcctggc cttgtccctg acggccgacc
     3841 agatggtcag tgccttgttg gatgctgagc cccccatact ctattccgag tatgatccta
     3901 ccagaccctt cagtgaagct tcgatgatgg gcttactgac caacctggca gacagggagc
     3961 tggttcacat gatcaactgg gcgaagaggg tgccaggctt tgtggatttg accctccatg
     4021 atcaggtcca ccttctagaa tgtgcctggc tagagatcct gatgattggt ctcgtctggc
     4081 gctccatgga gcacccagtg aagctactgt ttgctcctaa cttgctcttg gacaggaacc
     4141 agggaaaatg tgtagagggc atggtggaga tcttcgacat gctgctggct acatcatctc
     4201 ggttccgcat gatgaatctg cagggagagg agtttgtgtg cctcaaatct attattttgc
     4261 ttaattctgg agtgtacaca tttctgtcca gcaccctgaa gtctctggaa gagaaggacc
     4321 atatccaccg agtcctggac aagatcacag acactttgat ccacctgatg gccaaggcag
     4381 gcctgaccct gcagcagcag caccagcggc tggcccagct cctcctcatc ctctcccaca
     4441 tcaggcacat gagtaacaaa ggcatggagc atctgtacag catgaagtgc aagaacgtgg
     4501 tgcccctcta tgacctgctg ctggaggcgg cggacgccca ccgcctacat gcgcccacta
     4561 gccgtggagg ggcatccgtg gaggagacgg accaaagcca cttggccact gcgggctcta
     4621 cttcatcgca ttccttgcaa aagtattaca tcacggggga ggcagagggt ttccctgcca
     4681 cagcttgata gcggccgcga gctcgctgat cagcctcgac tgtgccttct agttgccagc
     4741 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     4801 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     4861 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     4921 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctcgatcc
     4981 tctagttggc gcgccaacgg taccagcttt tgttcccttt agtgagggtt aatttcgagc
     5041 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5101 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5161 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     5221 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5281 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5341 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5401 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5461 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5521 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5581 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5641 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     5701 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     5761 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     5821 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     5881 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     5941 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6001 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6061 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6121 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6181 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6241 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6301 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6361 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6421 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6481 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6541 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6601 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6661 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     6721 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     6781 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     6841 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     6901 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     6961 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7021 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7081 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7141 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacattyms cckraawgks
     7201 smayskramk ygtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     7261 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     7321 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7381 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     7441 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     7501 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     7561 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcagtcacg ctgcgcgtaa
     7621 ccaccacacc cgccgcgctt aatgcgccgc tacaggrcrm ktymsrtkcg ccattcaggc
     7681 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     7741 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     7801 gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa ttggccttaa
     7861 ttaagggatc tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg
     7921 tccctttttt ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtgggg
     7981 atcgacggta tcgtagagtc gaggccgctc tagaactagt tgccgatttc ggcctattgg
     8041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     8101 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8161 tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     8221 aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc
     8281 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt
     8341 atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt
     8401 ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg
     8461 atcagcacgt gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa
     8521 ggtgaggaac taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
     8581 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
     8641 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
     8701 ttgtgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
     8761 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
     8821 ggtgcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
     8881 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcact
     8941 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     9001 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     9061 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     9121 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     9181 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ct
//
