LOCUS       sequence-310089-        3687 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-NSP6
FEATURES             Location/Qualifiers
     source          1..3687
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     101..120
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     354..371
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(530..557)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(649..735)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     784..806
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     798..815
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     799..815
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    831..930
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             930..950
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(1828..1927)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1944..1963)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1945..1963)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1968..1984)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1968..1984)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             2415..3206
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(3299..3687,1..200)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
       61 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
      121 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
      181 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
      241 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
      301 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
      361 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
      421 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
      481 acgcaattaa tacgcgtacc gctagccagg aagagtttgt agaaacgcaa aaaggccatc
      541 cgtcaggatg gccttctgct tagtttgatg cctggcagtt tatggcgggc gtcctgcccg
      601 ccaccctccg ggccgttgct tcacaacgtt caaatccgct cccggcggat ttgtcctact
      661 caggagagcg ttcaccgaca aacaacagat aaaacgaaag gcccagtctt ccgactgagc
      721 ctttcgtttt atttgatgcc tggcagttcc ctactctcgc gttaacgcta gcatggatgt
      781 tttcccagtc acgacgttgt aaaacgacgg ccagtcttaa gctcgggccc caaataatga
      841 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
      901 taatgccaac tttgtacaaa aaagttggcg aaaacctgta cttccaaggc atgagcgccg
      961 tgaagagaac catcaaggga acccaccact ggctgctgct gaccatcctg acctccctgc
     1021 tggtgctggt gcagtctacc cagtggagcc tgttcttctt cctgtacgag aacgccttcc
     1081 tgcccttcgc tatgggtatc atcgccatgt ccgccttcgc tatgatgttc gtgaagcaca
     1141 agcacgcttt cctgtgcctg ttcctgctgc catccctggc caccgtggct tacttcaaca
     1201 tggtgtacat gcccgcctct tgggtcatgc gcatcatgac ctggctggac atggtggaca
     1261 ccagcctgtc cggcttcaag ctgaaggact gcgtgatgta cgcctctgct gtggtgctgc
     1321 tgatcctgat gaccgctaga accgtgtacg atgatggtgc tagaagggtg tggaccctga
     1381 tgaacgtgct gaccctggtg tacaaggtgt actacggcaa cgccctggac caggctatca
     1441 gcatgtgggc tctgatcatc tctgtgacca gcaactactc cggagtggtg accaccgtga
     1501 tgttcctggc cagaggtatc gtgttcatgt gcgtggagta ctgccctatc ttcttcatca
     1561 ccggcaacac cctgcagtgc atcatgctgg tgtactgctt cctgggttac ttctgcacct
     1621 gctatttcgg cctgttctgc ctgctgaaca gatacttccg tctgaccctg ggcgtgtacg
     1681 actacctggt gtccacccag gaattccgtt acatgaactc tcagggactg ctgcccccta
     1741 agaacagcat cgacgctttc aagctgaaca tcaagctgct gggagtgggc ggaaagccat
     1801 gcatcaaggt ggccaccgtg cagtaatacc caactttctt gtacaaagtt ggcattataa
     1861 gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa aataaaatca
     1921 ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc atagctgttt
     1981 cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata
     2041 tatcatcatg cctcctctag accagccagg acagaaatgc ctcgacttcg ctgctaccca
     2101 aggttgccgg gtgacgcaca ccgtggaaac ggatgaaggc acgaacccag tggacataag
     2161 cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac tggtccagaa
     2221 ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct tgttatgact
     2281 gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt tacgccgtgg
     2341 gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt cgccctaaaa
     2401 caaagttaaa cattatgagg gaagcggtga tcgccgaagt atcgactcaa ctatcagagg
     2461 tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat ttgtacggct
     2521 ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt acggtgaccg
     2581 taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa acttcggctt
     2641 cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg cacgacgaca
     2701 tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg cagcgcaatg
     2761 acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct atcttgctga
     2821 caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa ctctttgatc
     2881 cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta tggaactcgc
     2941 cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc atttggtaca
     3001 gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca atggagcgcc
     3061 tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt ggacaagaag
     3121 aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac gtgaaaggcg
     3181 agatcaccaa ggtagtcggc aaataaccct cgagccaccc atgaccaaaa tcccttaacg
     3241 tgagttacgc gtcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3301 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3361 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3421 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3481 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3541 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3601 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3661 acaccgaact gagataccta cagcgtg
//
