LOCUS       sequence-310092-        8521 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  tamoxifen-inducible Flp recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8521)
  AUTHORS   Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB,
            Schar P, Schuermann D
  TITLE     Inducible TDG knockout models to study epigenetic regulation.
  JOURNAL   F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. 
            eCollection 2020.
  PUBMED    33082936
REFERENCE   2  (bases 1 to 8521)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2020-09-9"; volume: "9:1112. doi"; pages: " 
            10.12688/f1000research.25637.2. eCollection 2020"
COMMENT     Sequence Label: pBigTB-FlpERT2
FEATURES             Location/Qualifiers
     source          1..8521
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..657
                     /label=chicken beta-actin promoter
     intron          658..1674
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1598..1620
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1682..1701
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1741..3009
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     CDS             3028..3963
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3076..3969
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     primer_bind     complement(3995..4012)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4001..4225
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(4297..4315)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4336..4352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4336..4352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4349..4371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4360..4376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4384..4390,4391..4408,4409..4414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4429..4450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4567..4584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4738..5326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4818..4837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5497..6288,6289..6357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6120..6139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6358..6462)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6489..6944
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6576..6595)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6786..6807
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7070..7092
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7084..7101
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7085..7101
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7108..7127
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7108..7126
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(7154..7219,7220..7257)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(7370..7390)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7375..7704
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7555..7690
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7617..7636
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7752..7799
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7818..8216
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    8374..8495
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8411..8430)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8465..8484
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
      541 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
      601 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
      661 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
      721 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
      781 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
      841 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcgggggg tgcgtgcgtg
      901 tgtgtgtgcg tggggagcgc cgcgtgcggc tccgcgctgc ccggcggctg tgagcgctgc
      961 gggcgcggcg cggggctttg tgcgctccgc agtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg gggctgcgag gggaacaaag gctgcgtgcg gggtgtgtgc
     1081 gtgggggggt gagcaggggg tgtgggcgcg tcggtcgggc tgcaaccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtac ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gcccccggag cgccggcggc
     1321 tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga gagggcgcag
     1381 ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc
     1441 tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg cggggagggc
     1501 cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg
     1561 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1621 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1681 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattctg agccgccacc
     1741 atgagccaat ttgatatatt atgtaaaaca ccacctaagg tcctggttcg tcagtttgtg
     1801 gaaaggtttg aaagaccttc aggggaaaaa atagcatcat gtgctgctga actaacctat
     1861 ttatgttgga tgattactca taacggaaca gcaatcaaga gagccacatt catgagctat
     1921 aatactatca taagcaattc gctgagtttc gatattgtca acaaatcact ccagtttaaa
     1981 tacaagacgc aaaaagcaac aattctggaa gcctcattaa agaaattaat tcctgcttgg
     2041 gaatttacaa ttattcctta caatggacaa aaacatcaat ctgatatcac tgatattgta
     2101 agtagtttgc aattacagtt cgaatcatcg gaagaagcag ataagggaaa tagccacagt
     2161 aaaaaaatgc ttaaagcact tctaagtgag ggtgaaagca tctgggagat cactgagaaa
     2221 atactaaatt cgtttgagta tacctcgaga tttacaaaaa caaaaacttt ataccaattc
     2281 ctcttcctag ctactttcat caattgtgga agattcagcg atattaagaa cgttgatccg
     2341 aaatcattta aattagtcca aaataagtat ctgggagtaa taatccagtg tttagtgaca
     2401 gagacaaaga caagcgttag taggcacata tacttcttta gcgcaagggg taggatcgat
     2461 ccacttgtat atttggatga atttttgagg aactctgaac cagtcctaaa acgagtaaat
     2521 aggaccggca attcttcaag caacaaacag gaataccaat tattaaaaga taacttagtc
     2581 agatcgtaca acaaggcttt gaagaaaaat gcgccttatc caatctttgc tataaagaat
     2641 ggcccaaaat ctcacattgg aagacatttg atgacctcat ttctgtcaat gaagggccta
     2701 acggagttga ctaatgttgt gggaaattgg agcgataagc gtgcttctgc cgtggccagg
     2761 acaacgtata ctcatcagat aacagcaata cctgatcact acttcgcact agtttctcgg
     2821 tactatgcat atgatccaat atcaaaggaa atgatagcat tgaaggatga gactaatcca
     2881 attgaggagt ggcagcatat agaacagcta aagggtagtg ctgaaggaag catacgatac
     2941 cccgcatgga atgggataat atcacaggag gtactagact acctttcatc ctacataaat
     3001 agacgcatat gcgtacgcgg atcctctgct ggagacatga gagctgccaa cctttggcca
     3061 agcccgctca tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac
     3121 cagatggtca gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct
     3181 accagaccct tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag
     3241 ctggttcaca tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat
     3301 gatcaggtcc accttctaga atgtgcctgg ctagagatcc tgatgattgg tctcgtctgg
     3361 cgctccatgg agcacccagt gaagctactg tttgctccta acttgctctt ggacaggaac
     3421 cagggaaaat gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct
     3481 cggttccgca tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg
     3541 cttaattctg gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac
     3601 catatccacc gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca
     3661 ggcctgaccc tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac
     3721 atcaggcaca tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg
     3781 gtgcccctct atgacctgct gctggaggcg gcggacgccc accgcctaca tgcgcccact
     3841 agccgtggag gggcatccgt ggaggagacg gaccaaagcc acttggccac tgcgggctct
     3901 acttcatcgc attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc
     3961 acagcttgat agcggccgcg agctcgctga tcagcctcga ctgtgccttc tagttgccag
     4021 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     4081 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     4141 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     4201 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgatc
     4261 ctctagttgg cgcgccaacg gtaccagctt ttgttccctt tagtgagggt taatttcgag
     4321 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4381 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4441 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4501 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4561 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4621 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4681 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4741 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4801 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4861 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4921 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4981 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5041 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5101 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5161 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5221 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5281 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5341 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5401 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5461 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5521 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5581 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5641 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5701 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     5761 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     5821 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     5881 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     5941 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6001 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6061 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6121 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6181 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6241 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6301 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6361 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6421 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6481 gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6541 cgtgactgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6601 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6661 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6721 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6781 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6841 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6901 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg
     6961 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7021 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     7081 cgttgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggcga attggcctta
     7141 attaagggat ctgtagggcg cagtagtcca gggtttcctt gatgatgtca tacttatcct
     7201 gtcccttttt tttccacagc tcgcggttga ggacaaactc ttcgcggtct ttccagtggg
     7261 gatcgacggt atcgtagagt cgaggccgct ctagaactag ttgccgattt cggcctattg
     7321 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     7381 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     7441 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     7501 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     7561 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     7621 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     7681 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     7741 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     7801 aggtgaggaa ctaaaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa
     7861 gagcaacggc tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag
     7921 ctctctctag cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac
     7981 cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt
     8041 gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac
     8101 aggtgcttct cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc
     8161 cgacggcagt tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagcac
     8221 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8281 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8341 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8401 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8461 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgctagca tcgataccgt
     8521 c
//
