LOCUS       sequence-310095-        3639 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Kim DK, Knapp JJ, Kuang D, Chawla A, Cassonnet P, Lee H, 
            Sheykhkarimli D, Samavarchi-Tehrani P, Abdouni H, Rayhan A, Li R, 
            Pogoutse O, Coyaud E, van der Werf S, Demeret C, Gingras AC, Taipale
            M, Raught B, Jacob Y, Roth FP
  TITLE     A Comprehensive, Flexible Collection of SARS-CoV-2 Coding Regions.
  JOURNAL   G3 (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554.
  PUBMED    32763951
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda). 2020 Aug 6. pii: g3.120.401554. doi: 
            10.1534/g3.120.401554."
COMMENT     Sequence Label: pDONR223 SARS-CoV2 TEV-ORF3A
FEATURES             Location/Qualifiers
     source          1..3639
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(25..42)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(196..784)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(276..295)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(877..1668)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2099..2115
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2099..2115
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2120..2139
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2120..2138
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2156..2255
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(3085..3105)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(3105..3204)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3220..3237)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3220..3236)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3229..3251)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3300..3386
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3478..3505
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
       61 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
      121 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
      181 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
      241 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
      301 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
      361 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
      421 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
      481 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
      541 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
      601 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
      661 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
      721 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
      781 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg acgcgtaact
      841 cacgttaagg gattttggtc atgggtggct cgagggttat ttgccgacta ccttggtgat
      901 ctcgcctttc acgtagtgga caaattcttc caactgatct gcgcgcgagg ccaagcgatc
      961 ttcttcttgt ccaagataag cctgtctagc ttcaagtatg acgggctgat actgggccgg
     1021 caggcgctcc attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc
     1081 gctgtaccaa atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg
     1141 cggcgagttc catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac
     1201 cggatcaaag agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt
     1261 tgtcagcaag atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat
     1321 gtcattgcgc tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat
     1381 gatgtcgtcg tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg
     1441 ggaagccgaa gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct
     1501 tacggtcacc gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc
     1561 ggagccgtac aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac
     1621 tacctctgat agttgagtcg atacttcggc gatcaccgct tccctcataa tgtttaactt
     1681 tgttttaggg cgactgccct gctgcgtaac atcgttgctg ctccataaca tcaaacatcg
     1741 acccacggcg taacgcgctt gctgcttgga tgcccgaggc atagactgta ccccaaaaaa
     1801 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
     1861 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagcttac gaaccgaaca
     1921 ggcttatgtc cactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
     1981 cttgggtagc agcgaagtcg aggcatttct gtcctggctg gtctagagga ggcatgatga
     2041 tatattttta tcttgtgcaa tgtaacatca gagattttga gacacgggcc agagctgcca
     2101 ggaaacagct atgaccatgt aatacgactc actatagggg atatcagctg gatggcaaat
     2161 aatgatttta ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt
     2221 tcttataatg ccaactttgt acaagaaagt tgggtattac agtggcacgg aggtggtggt
     2281 ggtaggttcg tcgtagatag gctccatcac ggggttcacc acgccgctag acacgtcgat
     2341 ggtgtggatc tgcacgtgtt cctcgggttc gtccacgatc ttgttgtaga tgaagaaggt
     2401 cacgtgctcc acaccggtgt cggtagacag ctgggtggag tacagctggt agtagtcgct
     2461 ggtgaagtaa gagtgcagca ccacgcagtc cttcactcca gattcccact tctcggtgta
     2521 tccgccgatc tggtagtcgt gttcggagat agggctggtg gtgccgtcac cggaggtgat
     2581 cacgatggag ctggtcacgc tgttgtaggg gatgcagtag tcgtagcagt tggtgtgcca
     2641 gcacaggaag tagttggcgt cgtacagcag ggggttcttg gatctgcact tccagcacag
     2701 ccacagcctc atgatgatgc gcacgaagtt gatgctctgc aggaagtaca ccagagcgta
     2761 caggtacagg aatggagcct ccagaccagc agccaccagc agcaggtgtg agtacacggt
     2821 cacgaacagc agcagcaggt tgcacacgaa gtgcacaccc ttggacagag ccagctgcca
     2881 cctcttcttc agggtgatga tcttagaggc ggactggaac acggccagca gggccacacc
     2941 cacgatcagc catccgaagg gcaggctagc ctggattggg atggtagcgg tggcacgcac
     3001 gaagtcagaa ggggtggcgt ccttgatctc tccctgcttc agggtcacgg tgccgatggt
     3061 gaagattctc atgaacaggt ccatgccttg gaagtacagg ttttcgccaa cttttttgta
     3121 caaagttggc attataaaaa agcattgctc atcaatttgt tgcaacgaac aggtcactat
     3181 cagtcaaaat aaaatcatta tttggggccc gagcttaaga ctggccgtcg ttttacaacg
     3241 tcgtgactgg gaaaacatcc atgctagcgt taacgcgaga gtagggaact gccaggcatc
     3301 aaataaaacg aaaggctcag tcggaagact gggcctttcg ttttatctgt tgtttgtcgg
     3361 tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gtgaagcaac
     3421 ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa actaagcaga
     3481 aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttcctggc tagcggtacg
     3541 cgtattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     3601 gcattaatga atcggccaac gcgcggggag aggcggttt
//
