LOCUS       sequence-31010-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Tolonen AC, Chilaka AC, Church GM
  TITLE     Targeted gene inactivation in Clostridium phytofermentans shows that
            cellulose degradation requires the family 9 hydrolase Cphy3367.
  JOURNAL   Mol Microbiol. 2009 Dec . 74(6):1300-13.
  PUBMED    19775243
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Microbiol. 2009 Dec . 74(6):1300-13."
COMMENT     Sequence Label: pQexp
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(453..470)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(453..469)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(462..484)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gcggttggca catttacctc taatcttcta gttcatttat atgtccatga attgaatttc
       61 atacatagtt ttcaattgta tggatgtagt atgacagaaa atcaaaaaat catacttatt
      121 atatttaggt agttactgtt aatttttagt atgttaattt ctaatctgag ttctttccgt
      181 attataataa gtgtaaagat cacaccatat ttttacagtt tttacacgtt tcattaataa
      241 tttcttatat ttctttattt gtttgtaaaa tttacttaaa tttcgccaga aaacaaaaga
      301 aagcctttac taattaatag tttagtgata ctcttttatg taggtatttt ttaaaataca
      361 ttaaacctag gtaattgagg aaagttacaa ttaccattat ataaggagga tattcaaacc
      421 atatgggatc cccgggtacc gagctcgaat tcactggccg tcgttttaca acgtcgtgac
      481 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      541 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      601 ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc
      661 ataggggatc ggtcttgcct tgctcgtcgg tgatgtactt caccagctcc gcgaagtcgc
      721 tcttcttgat ggagcgcatg ggggacgtgc ttggcaatca cgcgcacccc ccggccgttt
      781 tagcggctaa aaaagtcatg gctctgccct cgggcggacc acgcccatca tgaccttgcc
      841 aagctcgtcc tgcttctctt cgatcttcgc cagcagggcg aggatcgt
//
