LOCUS       sequence-310101-       12432 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  crRNA entry vector of Pfba1-BsaI pad-TEF1 Term with FnCas12a in 
            Schizosaccharomyces pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12432)
  AUTHORS   Zhao Y, Boeke JD
  TITLE     CRISPR-Cas12a system in fission yeast for multiplex genomic editing 
            and CRISPR interference.
  JOURNAL   Nucleic Acids Res. 2020 Jun 4;48(10):5788-5798. doi: 
            10.1093/nar/gkaa329.
  PUBMED    32374858
REFERENCE   2  (bases 1 to 12432)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2020-06-4- 4"; volume: "48"; issue: 
            "10"; pages: "5788-5798"
COMMENT     Sequence Label: pYZ673
FEATURES             Location/Qualifiers
     source          1..12432
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..259
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        240..258
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      1573..1770
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     primer_bind     complement(1831..1848)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1831..1847)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1840..1862)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1988..2443
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2075..2094)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2285..2306
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(2879..3673)
                     /codon_start=1
                     /gene="Schizosaccharomyces pombe ura4"
                     /product="orotidine 5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=S. pombe ura4
                     /translation="MDARVFQSYSARAEGMKNPIAKELLALMEEKQSNLSVAVDLTKKS
                     EILELVDKIGPYVCVIKTHIDVVEDFDQDMVEKLVALGKKHRFLIFEDRKFADIGNTVK
                     LQYASGVYKIASWAHITNCHTVPGEGIIQGLKEVGLPLGRGLLLLAEMSSKGSLATGSY
                     TEKTLEWFEKHTDFCFGFIAGRRFPNLQSDYITMSPGIGLDVKGDGLGQQYRTPEEVIV
                     NCGSDIIIVGRGVYGAGRNPVVEAKRYREAGWKAYQQRLSQH"
     rep_origin      4293..5076
                     /label=ars1
                     /note="Schizosaccharomyces pombe autonomously replicating 
                     sequence ars1"
     promoter        5262..6003
                     /label=adh1 promoter
                     /note="promoter for the Schizosaccharomyces pombe adh1 gene
                     encoding alcohol dehydrogenase"
     CDS             9924..9971
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             9978..10058
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     terminator      10074..10321
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(10090..10108)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     promoter        10527..10631
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10632..10700,10701..11492)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10850..10869)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11663..12251
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12152..12171
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12405..12422
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct
      241 aatacgactc actataggga aagcttgcat gcctgcaggt cgactctaga ggatcggccg
      301 gcccgcggcc gcacagtctc atactcttct agtcaagtct catcctcttc cactctctca
      361 attccttttt atcaatgttt cttcctcttg ttatgtacac aaacgatgat ctcttctctc
      421 acctcattgc tggactaata ttactcgtgg ccaagcgaaa cacaagtggt actagataac
      481 caaaaaatga taaagcattc aatcaatcaa accacttacg aaattcatac tcccgtaaac
      541 gaatgccggt cggacgcatg gaaaatcatc aatacgtgag agagctactt agttgtgcac
      601 tttgtatatg tccaaaaata cttttcattt cacatacctg cttgtgtaaa gacagtgatg
      661 aaatgcggtg caaagtgtct attcttacat tgatggtaaa gctaattcaa gcatgcttgg
      721 aagaagaatt gccttgcatt tcttgatcat ttgacccagg cgacaactat aaaataagaa
      781 tactaccgaa gatgcagatg gggtgtgtgg gcacaacacc atcgaaccga accgagacgg
      841 ccagactgtg ataacaaaag aaatgcatta gtttagttgg ctggttcaag ttggttgagt
      901 agaaaaatcc atcactcaaa ctgggaagta gggatggctg ggagaatgga gaagcgagac
      961 gagacgagac aagacaagag gagatgaaat gaaattaatg aaaagaaatg aatagaaatg
     1021 aaatagacat atgaatagta tagggcaggt tagatgtttg tatttataaa tgtttaatgc
     1081 ttaggtttgt atttatgttt acgttggtaa tgtttctttt gtttgtgttt gtgcatcggg
     1141 tgaacgagtt ggttaccgcc gccgccgctt ccttttgcct tgacacacac cacgcaacgt
     1201 gttacggttc ttgaacggaa ttgcgtactt cgatgcagag cgctatcggg gggattgggg
     1261 atggtaaatg gaagaaatca aaaacttctt ttgacgtcaa cccctaaaac aggggacggt
     1321 gattggtggt aaccacggac cggataggta tcgatatgca catcaggtga gaagtttttc
     1381 cgaggccaac tccggctcca tggtatataa tagagggttg cgcgctactg ttgcagtatc
     1441 atacttccaa aggtcgtata gaacacacac acaaattagc aaaatgaatt tctactgttg
     1501 tagatcgaga cctccctgca agaccatcta caaagttggt ctccaatttc tactgttgta
     1561 gattgagtca gtactgacaa taaaaagatt cttgttttca agaacttgtc atttgtatag
     1621 tttttttata ttgtagttgt tctattttaa tcaaatgtta gcgtgattta tatttttttt
     1681 cgcctcgaca tcatctgccc agatgcgaag ttaagtgcgc agaaagtaat atcatgcgtc
     1741 aatcgtatgt gaatgctggt cgctatactg ctgtcgattc gatactaacg ccgccatcca
     1801 gtgtcgaagc ggccgcgcga gctcgaattc actggccgtc gttttacaac gtcgtgactg
     1861 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1921 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     1981 cgaatggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     2041 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     2101 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     2161 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     2221 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     2281 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     2341 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     2401 aaatttaacg cgaattttaa caaaatatta acgtttacaa tttagcttgt gatattgacg
     2461 aaactttttg acatctaatt tattctgttc caacaccaat gtttataacc aagttttatc
     2521 ttgtttgtct acatggtatt ttacattcat ctacatacat ctttcattgg ctttgtacat
     2581 agttatcatt acaagtctaa aaaaattcac tcttttctta ttcaatgtca atccaagaga
     2641 aaagattgtg gtaatgttgt aggagcatgt ttaataaatt actatagcaa attacttttt
     2701 attcccaagg tgtttatcta taatagttaa tattttagtc gctacataaa attttaccaa
     2761 agagtacttg tatactaatt ctaaatgcct tctgacataa aacgcctagg aaaacaaacg
     2821 caaacaaggc atcgactttt tcaataacca accaaaaaaa ttttacatta gtcttttttt
     2881 aatgctgaga aagtctttgc tgatatgcct tccaaccagc ttctctatat ctcttggctt
     2941 cgacaacagg attacgacca gctccataga ctccacgacc aacaatgatg atatcgctac
     3001 cgcagtttac aatcacttct tcaggagtac gatattgctg tcccagcccg tctcctttaa
     3061 catccaagcc gataccaggg gacatagtta tgtagtcgct ttgaaggtta ggaaatcgac
     3121 gaccagctat aaagccaaag caaaaatcgg tatgcttctc aaaccattct aaggttttct
     3181 ctgtgtagga accagtagcc aaagagcctt tggaagacat ttcagccaaa agcaagagtc
     3241 cacgtcccaa aggtaaacca acttctttga ggccttgtat aataccctcg cctggcactg
     3301 tatggcaatt tgtgatatga gcccaagaag caattttgta cacaccagat gcatattgta
     3361 gcttgacggt atttccaatg tctgcgaatt tgcgatcctc aaagataaga aaacgatgct
     3421 ttttacctaa ggccaccagt ttttctacca tatcctggtc gaaatcctcg acaacgtcaa
     3481 tatgtgtctt gataacacag acatagggtc caattttatc taccaattct aagatttcgg
     3541 atttcttcgt caaatcgacc gcgactgaca agttgctttg cttttcttcc atcaaagcca
     3601 acaattcctt ggcaatggga tttttcatcc cctcagctct agctgaatag ctttgaaata
     3661 ctctagcatc cataactttg cttttaaacc tttaatttcg atccaagcaa aaaagaggtt
     3721 cttggtagga caatacggta agaaaacacg acatgtgcag agatgccgac gaagcatagt
     3781 taaactggga tggtaaaatc aattaagaat ttataaagac aaaattgtat aagtctctaa
     3841 aacatcttaa ttatacctca cagaactatc taaaatatat tcacaaagtg caaacattat
     3901 catgaaaaag aaccatttta atttaaagca agggcattaa ggcttattta cagaatttct
     3961 tacttttgta aagattataa ggctgattat ctttttcacc atgccaaaaa ttacacaaga
     4021 tagaatggat gtttgaaatt aaacgtgagt atacaaacaa atacactagg taaatcgaaa
     4081 catttttttc tccattaagt aacaaattcc tatttagaga aagaatgctg agtagattaa
     4141 ataatctata caaacttttt taacacaaat gcatacatat agccagtggg atttgtagct
     4201 agtcgatcga ctctagagga aattcgagtc taactcctta accactcgga catagtgact
     4261 tatctgacac tcattgtaaa ttaatatata tataggcatt ttgtttagtt aaaggtactt
     4321 aagtaattag tataaacgaa ccaattttat aatcaggaag ttaagtgaat ggtagcacat
     4381 gtcgtaaaaa ttgtgaattt ttattgaata atattttaaa tacaagcctt tctagactag
     4441 gtatactcat aaacatatat gagcaaaagg atagaggaga ttacattgca tcttctacaa
     4501 atttatttat tgccctttac tgaaaaatta aataatgagt actaaatgat aaaaagcgct
     4561 cagtacaaga aagattgcaa aaatattgca ttcttcatga attaaagttg catataaggc
     4621 atattgaaag taatagtact aaaacagcag ttagcgaaaa ttaatagaat tatattcgca
     4681 agacaattgt gacattaaat taaaaaattg tagaattttt actatcctct ttaacgccat
     4741 gagcctttat aaaaaggtta aattagtttt aacattcttt ttttgagtaa gagtttacaa
     4801 tttatcaaaa cctgtgttat tatattcatt agtttcaatt tattagcatc tagagaaaaa
     4861 tcaattggca gttacattgt tggaatttat gaagaaaaag aatctacaac ggagaataag
     4921 ttgctgatcg ctttccctaa aattgtatat tttgctgagc ttattttgac atttcgttga
     4981 agttttctct aattcgcatt cattttaagt aaaacaatga gaaataaaat tacaaaaaat
     5041 acaaattaaa atacaatttt tagctataat tatagacgat gcccttgtat cccattctgt
     5101 ctcgcttgcc cctacttttt atcttttata taccataatg aacgctgccg ctactaacca
     5161 taccccgatt ttacatttcg gactcccaag gacgtacaaa atagaaaact atagaaaaaa
     5221 ataatcagaa aatagcatgt catctctttg taaaacgcag tgccctacaa caactaagaa
     5281 aatggctatc atgcggaagc tggtgagaag aacagcatcg ggacaaggga aggaagaaca
     5341 aagacaaaga aaacaaaaga aagcaattga aaacaaaaca aaacaatttt cattccttct
     5401 cttatcattc cttttctttt cttttctctc attcaacgca ctccatcgta tccgtattcc
     5461 tcttattttt tctctttctc tatatccatt tctttctctc taggtgtgtc ctctctctct
     5521 cttcaatttc tctactccgc attccaacgc atccttcccc caacctccca tttcctcctt
     5581 acggcccgat agcgatcgtc tttccctcgc tatcactcgc taccggcccc tcctctgcac
     5641 cgtaacctcc tacgtattta ccatatcata aagttttttc cgacgcttat cgctgacccc
     5701 ctgtcgccct cctattggct tccggattat cttcttgtcc ataaggtgat ccatgcttcc
     5761 tgaagattcc cgaaatgtgt ccactttggc ggggaatcat tccatccact tctttctctc
     5821 tcgctttcct cattcggcgc tccccttccg cgtctcattg gtcttccgct ccgtttttgc
     5881 tttgccgatg ttacttgggg agaggtgcga taatcctttc gcaaaaactc ggtttgacgc
     5941 ctcccatggt ataaatagtg ggtggtggac aggtgccttc gcttttcttt aagcaagaga
     6001 attgctgcag gtcgactcta gagatgagca tctaccagga gttcgtcaac aagtattcac
     6061 tgagtaagac actgcggttc gagctgatcc cacagggcaa gacactggag aacatcaagg
     6121 cccgaggcct gattctggac gatgagaagc gggcaaaaga ctataagaaa gccaagcaga
     6181 tcattgataa ataccaccag ttctttatcg aggaaattct gagctccgtg tgcatcagtg
     6241 aggatctgct gcagaattac tcagacgtgt acttcaagct gaagaagagc gacgatgaca
     6301 acctgcagaa ggacttcaag tccgccaagg acaccatcaa gaaacagatt agcgagtaca
     6361 tcaaggactc cgaaaagttt aaaaatctgt tcaaccagaa tctgatcgat gctaagaaag
     6421 gccaggagtc cgacctgatc ctgtggctga aacagtctaa ggacaatggg attgaactgt
     6481 tcaaggctaa ctccgatatc actgatattg acgaggcact ggaaatcatc aagagcttca
     6541 agggatggac cacatacttt aaaggcttcc acgagaaccg caagaacgtg tactccagca
     6601 acgacattcc tacctccatc atctaccgaa tcgtcgatga caatctgcca aagttcctgg
     6661 agaacaaggc caaatatgaa tctctgaagg acaaagctcc cgaggcaatt aattacgaac
     6721 agatcaagaa agatctggct gaggaactga cattcgatat cgactataag actagcgagg
     6781 tgaaccagag ggtcttttcc ctggacgagg tgtttgaaat cgccaatttc aacaattacc
     6841 tgaaccagtc cggcattact aaattcaata ccatcattgg cgggaagttt gtgaacgggg
     6901 agaataccaa gcgcaaggga attaacgaat acatcaatct gtatagccag cagatcaacg
     6961 acaaaactct gaagaaatac aagatgtctg tgctgttcaa acagatcctg agtgataccg
     7021 agtccaagtc ttttgtcatt gataaactgg aagatgactc agacgtggtc actaccatgc
     7081 agagctttta tgagcagatc gccgctttca agacagtgga ggaaaaatct attaaggaaa
     7141 ctctgagtct gctgttcgat gacctgaaag cccagaagct ggacctgagt aagatctact
     7201 tcaaaaacga taagagtctg acagacctgt cacagcaggt gtttgatgac tattccgtga
     7261 ttgggaccgc cgtcctggag tacattacac agcagatcgc tccaaagaac ctggataatc
     7321 cctctaagaa agagcaggaa ctgatcgcta agaaaaccga gaaggcaaaa tatctgagtc
     7381 tggaaacaat taagctggca ctggaggagt tcaacaagca cagggatatt gacaaacagt
     7441 gccgctttga ggaaatcctg gccaacttcg cagccatccc catgattttt gatgagatcg
     7501 cccagaacaa agacaatctg gctcagatca gtattaagta ccagaaccag ggcaagaaag
     7561 acctgctgca ggcttcagca gaagatgacg tgaaagccat caaggatctg ctggaccaga
     7621 ccaacaatct gctgcacaag ctgaaaatct tccatattag tcagtcagag gataaggcta
     7681 atatcctgga taaagacgaa cacttctacc tggtgttcga ggaatgttac ttcgagctgg
     7741 caaacattgt ccccctgtat aacaagatta ggaactacat cacacagaag ccttactctg
     7801 acgagaagtt taaactgaac ttcgaaaata gtaccctggc caacgggtgg gataagaaca
     7861 aggagcctga caacacagct atcctgttca tcaaggatga caagtactat ctgggagtga
     7921 tgaataagaa aaacaataag atcttcgatg acaaagccat taaggagaac aaaggggaag
     7981 gatacaagaa aatcgtgtat aagctgctgc ccggcgcaaa taagatgctg cctaaggtgt
     8041 tcttcagcgc caagagtatc aaattctaca acccatccga ggacatcctg cggattagaa
     8101 atcactcaac acatactaag aacgggagcc cccagaaggg atatgagaaa tttgagttca
     8161 acatcgagga ttgcaggaag tttattgact tctacaagca gagcatctcc aaacaccctg
     8221 aatggaagga ttttggcttc cggttttccg acacacagag atataactct atcgacgagt
     8281 tctaccgcga ggtggaaaat caggggtata agctgacttt tgagaacatt tctgaaagtt
     8341 acatcgacag cgtggtcaat cagggaaagc tgtacctgtt ccagatctat aacaaagatt
     8401 tttcagcata cagcaagggc agaccaaacc tgcatacact gtactggaag gccctgttcg
     8461 atgagaggaa tctgcaggac gtggtctata aactgaacgg agaggccgaa ctgttttacc
     8521 ggaagcagtc tattcctaag aaaatcactc acccagctaa ggaggccatc gctaacaaga
     8581 acaaggacaa tcctaagaaa gagagcgtgt tcgaatacga tctgattaag gacaagcggt
     8641 tcaccgaaga taagttcttt ttccattgtc caatcaccat taacttcaag tcaagcggcg
     8701 ctaacaagtt caacgacgag atcaatctgc tgctgaagga aaaagcaaac gatgtgcaca
     8761 tcctgagcat tgaccgagga gagcggcatc tggcctacta taccctggtg gatggcaaag
     8821 ggaatatcat taagcaggat acattcaaca tcattggcaa tgaccggatg aaaaccaact
     8881 accacgataa actggctgca atcgagaagg atagagactc agctaggaag gactggaaga
     8941 aaatcaacaa cattaaggag atgaaggaag gctatctgag ccaggtggtc catgagattg
     9001 caaagctggt catcgaatac aatgccattg tggtgttcga ggatctgaac ttcggcttta
     9061 agagggggcg ctttaaggtg gaaaaacagg tctatcagaa gctggagaaa atgctgatcg
     9121 aaaagctgaa ttacctggtg tttaaagata acgagttcga caagaccgga ggcgtcctga
     9181 gagcctacca gctgacagct ccctttgaaa ctttcaagaa aatgggaaaa cagacaggca
     9241 tcatctacta tgtgccagcc ggattcactt ccaagatctg ccccgtgacc ggctttgtca
     9301 accagctgta ccctaaatat gagtcagtga gcaagtccca ggaatttttc agcaagttcg
     9361 ataagatctg ttataatctg gacaaggggt acttcgagtt ttccttcgat tacaagaact
     9421 tcggcgacaa ggccgctaag gggaaatgga ccattgcctc cttcggatct cgcctgatca
     9481 actttcgaaa ttccgataaa aaccacaatt gggacactag ggaggtgtac ccaaccaagg
     9541 agctggaaaa gctgctgaaa gactactcta tcgagtatgg acatggcgaa tgcatcaagg
     9601 cagccatctg tggcgagagt gataagaaat ttttcgccaa gctgacctca gtgctgaata
     9661 caatcctgca gatgcggaac tcaaagaccg ggacagaact ggactatctg attagccccg
     9721 tggctgatgt caacggaaac ttcttcgaca gcagacaggc acccaaaaat atgcctcagg
     9781 atgcagacgc caacggggcc taccacatcg ggctgaaggg actgatgctg ctgggccgga
     9841 tcaagaacaa tcaggagggg aagaagctga acctggtcat taagaacgag gaatacttcg
     9901 agtttgtcca gaatagaaat aacaaaaggc cggcggccac gaaaaaggcc ggccaggcaa
     9961 aaaagaaaaa gggatcctac ccatacgatg ttccagatta cgcttatccc tacgacgtgc
    10021 ctgattatgc atacccatat gatgtccccg actatgccta atctcttctc gagtcatgta
    10081 attagttatg tcacgcttac attcacgccc tccccccaca tccgctctaa ccgaaaagga
    10141 aggagttaga caacctgaag tctaggtccc tatttatttt tttatagtta tgttagtatt
    10201 aagaacgtta tttatatttc aaatttttct tttttttctg tacagacgcg tgtacgcatg
    10261 taacattata ctgaaaacct tgcttgagaa ggttttggga cgctcgaagg ctttaatttg
    10321 cgatcccctt ttcgtttgca agaagaaagg aaacaatgga gaagagatca tccatttgta
    10381 tgtaaatttt agtaaacttg aagaaatcac taacaacttc tcttacttag ggattctatg
    10441 caaaaccttg taaatcatct gatggaggac tcgatttaat gaattggaag gttggatttc
    10501 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
    10561 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
    10621 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
    10681 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
    10741 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
    10801 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
    10861 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
    10921 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
    10981 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
    11041 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
    11101 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
    11161 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
    11221 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
    11281 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
    11341 agcgtggatc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
    11401 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
    11461 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
    11521 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
    11581 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
    11641 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
    11701 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
    11761 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
    11821 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
    11881 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
    11941 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
    12001 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
    12061 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
    12121 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
    12181 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
    12241 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
    12301 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
    12361 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
    12421 aggaagcgga ag
//
