LOCUS       sequence-310102-       16286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ROSA26 targetting vector with tamoxifen-inducible Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16286)
  AUTHORS   Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB,
            Schar P, Schuermann D
  TITLE     Inducible TDG knockout models to study epigenetic regulation.
  JOURNAL   F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. 
            eCollection 2020.
  PUBMED    33082936
REFERENCE   2  (bases 1 to 16286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2020-09-9"; volume: "9:1112. doi"; pages: " 
            10.12688/f1000research.25637.2. eCollection 2020"
COMMENT     Sequence Label: pROSA26-ERT2CreERT2
FEATURES             Location/Qualifiers
     source          1..16286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          655..1671
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1595..1617
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1679..1698
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1737..1746
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1746..2681
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2691..3713
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="NLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKMLLS
                     VCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGLPR
                     PSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAFLG
                     IAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVERW
                     ISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQRY
                     LAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLEDG
                     D"
     CDS             3726..4661
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3774..4667
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    4699..4923
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4977..9317
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        9367..9866
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(9402..9425)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9799..9818
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9893..10465
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          10542..10607
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    10681..10905
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10967..10987)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10967..10985)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11006..11022)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11006..11022)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11019..11041)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11030..11046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11054..11060,11061..11078,11079..11084))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11099..11120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11237..11254)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11408..11996)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11488..11507)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12167..12958,12959..13027))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12790..12809
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13028..13132)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      13159..13614
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(13246..13265)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     13456..13477
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13740..13762
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13754..13771
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13755..13771
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13781..13800
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13781..13799
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    13818..14900
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(14919..14984,14985..15022)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(15135..15155)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        15140..15469
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      15320..15455
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     15382..15401
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        15517..15564
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             15583..15981
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    16139..16260
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(16176..16195)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     16230..16249
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggnnnnnnn cgcgcsaggc ggggcggggc ggggcgaggg gcggggcggg
      541 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
      601 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      661 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      721 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      781 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
      841 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
      901 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
      961 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     1021 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1081 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     1141 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     1201 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1261 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     1321 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1381 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     1441 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     1561 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1621 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1681 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattcccggg tgagccgcca
     1741 ccatggctgg agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct
     1801 ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg
     1861 atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt
     1921 cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg
     1981 cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac cttctagaat
     2041 gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga
     2101 agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca
     2161 tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc
     2221 agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat
     2281 ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca
     2341 agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc
     2401 accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag
     2461 gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc
     2521 tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg
     2581 aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat tccttgcaaa
     2641 agtattacat cacgggggag gcagagggtt tccctgccac agctgtcgac aatttactga
     2701 ccgtacacca aaatttgcct gcattaccgg tcgatgcaac gagtgatgag gttcgcaaga
     2761 acctgatgga catgttcagg gatcgccagg cgttttctga gcatacctgg aaaatgcttc
     2821 tgtccgtttg ccggtcgtgg gcggcatggt gcaagttgaa taaccggaaa tggtttcccg
     2881 cagaacctga agatgttcgc gattatcttc tatatcttca ggcgcgcggt ctggcagtaa
     2941 aaactatcca gcaacatttg ggccagctaa acatgcttca tcgtcggtcc gggctgccac
     3001 gaccaagtga cagcaatgct gtttcactgg ttatgcggcg gatccgaaaa gaaaacgttg
     3061 atgccggtga acgtgcaaaa caggctctag cgttcgaacg cactgatttc gaccaggttc
     3121 gttcactcat ggaaaatagc gatcgctgcc aggatatacg taatctggca tttctgggga
     3181 ttgcttataa caccctgtta cgtatagccg aaattgccag gatcagggtt aaagatatct
     3241 cacgtactga cggtgggaga atgttaatcc atattggcag aacgaaaacg ctggttagca
     3301 ccgcaggtgt agagaaggca cttagcctgg gggtaactaa actggtcgag cgatggattt
     3361 ccgtctctgg tgtagctgat gatccgaata actacctgtt ttgccgggtc agaaaaaatg
     3421 gtgttgccgc gccatctgcc accagccagc tatcaactcg cgccctggaa gggatttttg
     3481 aagcaactca tcgattgatt tacggcgcta aggatgactc tggtcagaga tacctggcct
     3541 ggtctggaca cagtgcccgt gtcggagccg cgcgagatat ggcccgcgct ggagtttcaa
     3601 taccggagat catgcaagct ggtggctgga ccaatgtaaa tattgtcatg aactatatcc
     3661 gtaacctgga tagtgaaaca ggggcaatgg tgcgcctgct ggaagatggc gatctcgagc
     3721 catctgctgg agacatgaga gctgccaacc tttggccaag cccgctcatg atcaaacgct
     3781 ctaagaagaa cagcctggcc ttgtccctga cggccgacca gatggtcagt gccttgttgg
     3841 atgctgagcc ccccatactc tattccgagt atgatcctac cagacccttc agtgaagctt
     3901 cgatgatggg cttactgacc aacctggcag acagggagct ggttcacatg atcaactggg
     3961 cgaagagggt gccaggcttt gtggatttga ccctccatga tcaggtccac cttctagaat
     4021 gtgcctggct agagatcctg atgattggtc tcgtctggcg ctccatggag cacccagtga
     4081 agctactgtt tgctcctaac ttgctcttgg acaggaacca gggaaaatgt gtagagggca
     4141 tggtggagat cttcgacatg ctgctggcta catcatctcg gttccgcatg atgaatctgc
     4201 agggagagga gtttgtgtgc ctcaaatcta ttattttgct taattctgga gtgtacacat
     4261 ttctgtccag caccctgaag tctctggaag agaaggacca tatccaccga gtcctggaca
     4321 agatcacaga cactttgatc cacctgatgg ccaaggcagg cctgaccctg cagcagcagc
     4381 accagcggct ggcccagctc ctcctcatcc tctcccacat caggcacatg agtaacaaag
     4441 gcatggagca tctgtacagc atgaagtgca agaacgtggt gcccctctat gacctgctgc
     4501 tggaggcggc ggacgcccac cgcctacatg cgcccactag ccgtggaggg gcatccgtgg
     4561 aggagacgga ccaaagccac ttggccactg cgggctctac ttcatcgcat tccttgcaaa
     4621 agtattacat cacgggggag gcagagggtt tccctgccac agcttgatag cggccgcgag
     4681 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4741 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4801 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4861 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4921 tggcttctga ggcggaaaga accagctggg gctcgatcct ctagttggcg cgccggctag
     4981 aagatgggcg ggagtcttct gggcaggctt aaaggctaac ctggtgtgtg ggcgttgtcc
     5041 tgcaggggaa ttgaacaggt gtaaaattgg agggacaaga cttcccacag attttcggtt
     5101 ttgtcgggaa gttttttaat aggggcaaat aaggaaaatg ggaggatagg tagtcatctg
     5161 gggttttatg cagcaaaact acaggttatt attgcttgtg atccgcctcg gagtattttc
     5221 catcgaggta gattaaagac atgctcaccc gagttttata ctctcctgct tgagatcctt
     5281 actacagtat gaaattacag tgtcgcgagt tagactatgt aagcagaatt ttaatcattt
     5341 ttaaagagcc cagtacttca tatccatttc tcccgctcct tctgcagcct tatcaaaagg
     5401 tattttagaa cactcatttt agccccattt tcatttatta tactggctta tccaacccct
     5461 agacagagca ttggcatttt ccctttcctg atcttagaag tctgatgact catgaaacca
     5521 gacagattag ttacatacac cacaaatcga ggctgtagct ggggcctcaa cactgcagtt
     5581 cttttataac tccttagtac actttttgtt gatcctttgc cttgatcctt aattttcagt
     5641 gtctatcacc tctcccgtca ggtggtgttc cacatttggg cctattctca gtccagggag
     5701 ttttacaaca atagatgtat tgagaatcca acctaaagct taactttcca ctcccatgaa
     5761 tgcctctctc ctttttctcc atttataaac tgagctatta accattaatg gtttccaggt
     5821 ggatgtctcc tcccccaata ttacctgatg tatcttacat attgccaggc tgatatttta
     5881 agacattaaa aggtatattt cattattgag ccacatggta ttgattactg cttactaaaa
     5941 ttttgtcatt gtacacatct gtaaaaggtg gttccttttg gaatgcaaag ttcaggtgtt
     6001 tgttgtcttt cctgacctaa ggtcttgtga gcttgtattt tttctattta agcagtgctt
     6061 tctcttggac tggcttgact catggcattc tacacgttat tgctggtcta aatgtgattt
     6121 tgccaagctt cttcaggacc tataattttg cttgacttgt agccaaacac aagtaaaatg
     6181 attaagcaac aaatgtattt gtgaagcttg gtttttaggt tgttgtgttg tgtgtgcttg
     6241 tgctctataa taatactatc caggggctgg agaggtggct cggagttcaa gagcacagac
     6301 tgctcttcca gaagtcctga gttcaattcc cagcaaccac atggtggctc acaaccatct
     6361 gtaatgggat ctgatgccct cttctggtgt gtctgaagac cacaagtgta ttcacattaa
     6421 ataaataaat cctccttctt cttctttttt ttttttttaa agagaatact gtctccagta
     6481 gaatttactg aagtaatgaa atactttgtg tttgttccaa tatggtagcc aataatcaaa
     6541 ttactcttta agcactggaa atgttaccaa ggaactaatt tttatttgaa gtgtaactgt
     6601 ggacagagga gccataactg cagacttgtg ggatacagaa gaccaatgca gactttaatg
     6661 tcttttctct tacactaagc aataaagaaa taaaaattga acttctagta tcctatttgt
     6721 ttaaactgct agctttactt aacttttgtg cttcatctat acaaagctga aagctaagtc
     6781 tgcagccatt actaaacatg aaagcaagta atgataattt tggatttcaa aaatgtaggg
     6841 ccagagttta gccagccagt ggtggtgctt gcctttatgc ctttaatccc agcactctgg
     6901 aggcagagac aggcagatct ctgagtttga gcccagcctg gtctacacat caagttctat
     6961 ctaggatagc caggaataca cacagaaacc ctgttgggga ggggggctct gagatttcat
     7021 aaaattataa ttgaagcatt ccctaatgag ccactatgga tgtggctaaa tccgtctacc
     7081 tttctgatga gatttgggta ttattttttc tgtctctgct gttggttggg tcttttgaca
     7141 ctgtgggctt tctttaaagc ctccttcctg ccatgtggtc tcttgtttgc tactaacttc
     7201 ccatggctta aatggcatgg ctttttgcct tctaagggca gctgctgaga tttgcagcct
     7261 gatttccagg gtggggttgg gaaatctttc aaacactaaa attgtccttt aatttttttt
     7321 ttaaaaaatg ggttatataa taaacctcat aaaatagtta tgaggagtga ggtggactaa
     7381 tattaaatga gtccctcccc tataaaagag ctattaaggc tttttgtctt atacttaact
     7441 ttttttttaa atgtggtatc tttagaacca agggtcttag agttttagta tacagaaact
     7501 gttgcatcgc ttaatcagat tttctagttt caaatccaga gaatccaaat tcttcacagc
     7561 caaagtcaaa ttaagaattt ctgactttta atgttaattt gcttactgtg aatataaaaa
     7621 tgatagcttt tcctgaggca gggtctcact atgtatctct gcctgatctg caacaagata
     7681 tgtagactaa agttctgcct gcttttgtct cctgaatact aaggttaaaa tgtagtaata
     7741 cttttggaac ttgcaggtca gattctttta taggggacac actaagggag cttgggtgat
     7801 agttggtaaa atgtgtttca agtgatgaaa acttgaatta ttatcaccgc aacctacttt
     7861 ttaaaaaaaa aagccaggcc tgttagagca tgcttaaggg atccctagga cttgctgagc
     7921 acacaagagt agttacttgg caggctcctg gtgagagcat atttcaaaaa acaaggcaga
     7981 caaccaagaa actacagtta aggttacctg tctttaaacc atctgcatat acacagggat
     8041 attaaaatat tccaaataat atttcattca agttttcccc catcaaattg ggacatggat
     8101 ttctccggtg aataggcaga gttggaaact aaacaaatgt tggttttgtg atttgtgaaa
     8161 ttgttttcaa gtgatagtta aagcccatga gatacagaac aaagctgcta tttcgaggtc
     8221 tcttggttta tactcagaag cacttctttg ggtttccctg cactatcctg atcatgtgct
     8281 aggcctacct taggctgatt gttgttcaaa taaacttaag tttcctgtca ggtgatgtca
     8341 tatgatttca tatatcaagg caaaacatgt tatatatgtt aaacatttgt acttaatgtg
     8401 aaagttaggt ctttgtgggt ttgattttta attttcaaaa cctgagctaa ataagtcatt
     8461 tttacatgtc ttacatttgg tggaattgta taattgtggt ttgcaggcaa gactctctga
     8521 cctagtaacc ctacctatag agcactttgc tgggtcacaa gtctaggagt caagcatttc
     8581 accttgaagt tgagacgttt tgttagtgta tactagttta tatgttggag gacatgttta
     8641 tccagaagat attcaggact atttttgact gggctaagga attgattctg attagcactg
     8701 ttagtgagca ttgagtggcc tttaggcttg aattggagtc acttgtatat ctcaaataat
     8761 gctggccttt tttaaaaagc ccttgttctt tatcaccctg ttttctacat aatttttgtt
     8821 caaagaaata cttgtttgga tctccttttg acaacaatag catgttttca agccatattt
     8881 tttttccttt tttttttttt ttttggtttt tcgagacagg gtttctctgt atagccctgg
     8941 ctgtcctgga actcactttg tagaccaggc tggcctcgaa ctcagaaatc cgcctgcctc
     9001 tgcctcctga gtgccgggat taaaggcgtg caccaccacg cctggctaag ttggatattt
     9061 tgttatataa ctataaccaa tactaactcc actgggtgga tttttaattc agtcagtagt
     9121 cttaagtggt ctttattggc ccttcattaa aatctactgt tcactctaac agaggctgtt
     9181 ggtactagtg gcacttaagc aacttcctac ggatatacta gcagattaag ggtcagggat
     9241 agaaactagt ctagcgtttt gtatacctac cagctttata ctaccttgtt ctgatagaaa
     9301 tatttcagga catctagctt atcgataccg tcgacggtat cgataagctt gatatcgaat
     9361 tctaccgggt aggggaggcg cttttcccaa ggcagtctgg agcatgcgct ttagcagccc
     9421 cgctgggcac ttggcgctac acaagtggcc tctggcctcg cacacattcc acatccaccg
     9481 gtaggcgcca accggctccg ttctttggtg gccccttcgc gccaccttct actcctcccc
     9541 tagtcaggaa gttccccccc gccccgcagc tcgcgtcgtg caggacgtga caaatggaag
     9601 tagcacgtct cactagtctc gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag
     9661 gcctttgggg cagcggccaa tagcagcttt gctccttcgc tttctgggct cagaggctgg
     9721 gaaggggtgg gtccgggggc gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa
     9781 ggtcctccgg aggcccggca ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct
     9841 cctcttcctc atctccgggc ctttcgacct gcaggtcctc gccatggatc ctgatgatgt
     9901 tgttgattct tctaaatctt ttgtgatgga aaacttttct tcgtaccacg ggactaaacc
     9961 tggttatgta gattccattc aaaaaggtat acaaaagcca aaatctggta cacaaggaaa
    10021 ttatgacgat gattggaaag ggttttatag taccgacaat aaatacgacg ctgcgggata
    10081 ctctgtagat aatgaaaacc cgctctctgg aaaagctgga ggcgtggtca aagtgacgta
    10141 tccaggactg acgaaggttc tcgcactaaa agtggataat gccgaaacta ttaagaaaga
    10201 gttaggttta agtctcactg aaccgttgat ggagcaagtc ggaacggaag agtttatcaa
    10261 aaggttcggt gatggtgctt cgcgtgtagt gctcagcctt cccttcgctg aggggagttc
    10321 tagcgttgaa tatattaata actgggaaca ggcgaaagcg ttaagcgtag aacttgagat
    10381 taattttgaa acccgtggaa aacgtggcca agatgcgatg tatgagtata tggctcaagc
    10441 ctgtgcagga aatcgtgtca ggcgatctct ttgtgaagga accttacttc tgtggtgtga
    10501 cataattgga caaactacct acagagattt aaagctctaa ggtaaatata aaatttttaa
    10561 gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt ccaacctatg
    10621 gaactgatga atgggagcag tggtggaatg cagatcctag agctcgctga tcagcctcga
    10681 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
    10741 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
    10801 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
    10861 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
    10921 gaaccagctg gggctcgagg gggggcccgg tacccagctt ttgttccctt tagtgagggt
    10981 taattgcgcg cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
    11041 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
    11101 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
    11161 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
    11221 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
    11281 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
    11341 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
    11401 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
    11461 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
    11521 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
    11581 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
    11641 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
    11701 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
    11761 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
    11821 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
    11881 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
    11941 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
    12001 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
    12061 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
    12121 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
    12181 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
    12241 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
    12301 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
    12361 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
    12421 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
    12481 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
    12541 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    12601 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    12661 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    12721 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    12781 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    12841 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    12901 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    12961 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    13021 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    13081 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    13141 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
    13201 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
    13261 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
    13321 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
    13381 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
    13441 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
    13501 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
    13561 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttccattc
    13621 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
    13681 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
    13741 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
    13801 cgaattggag ctccaccgcg gcaggccctc cgagcgtggt ggagccgttc tgtgagacag
    13861 ccgggtacga gtcgtgacgc tggaaggggc aagcgggtgg tgggcaggaa tgcggtccgc
    13921 cctgcagcaa ccggaggggg agggagaagg gagcggaaaa gtctccaccg gacgcggcca
    13981 tggctcgggg gggggggggc agcggaggag cgcttccggc cgacgtctcg tcgctgattg
    14041 gcttcttttc ctcccgccgt gtgtgaaaac acaaatggcg tgttttggtt ggcgtaaggc
    14101 gcctgtcagt taacggcagc cggagtgcgc agccgccggc agcctcgctc tgcccactgg
    14161 gtggggcggg aggtaggtgg ggtgaggcga gctggacgtg cgggcgcggt cggcctctgg
    14221 cggggcgggg gaggggaggg agggtcagcg aaagtagctc gcgcgcgagc ggccgcccac
    14281 cctccccttc ctctggggga gtcgttttac ccgccgccgg ccgggcctcg tcgtctgatt
    14341 ggctctcggg gcccagaaaa ctggcccttg ccattggctc gtgttcgtgc aagttgagtc
    14401 catccgccgg ccagcggggg cggcgaggag gcgctcccag gttccggccc tcccctcggc
    14461 cccgcgccgc agagtctggc cgcgcgcccc tgcgcaacgt ggcaggaagc gcgcgctggg
    14521 ggcggggacg ggcagtaggg ctgagcggct gcggggcggg tgcaagcacg tttccgactt
    14581 gagttgcctc aagaggggcg tgctgagcca gacctccatc gcgcactccg gggagtggag
    14641 ggaaggagcg agggctcagt tgggctgttt tggaggcagg aagcacttgc tctcccaaag
    14701 tcgctctgag ttgttatcag taagggagct gcagtggagt aggcggggag aaggccgcac
    14761 ccttctccgg aggggggagg ggagtgttgc aatacctttc tgggagttct ctgctgcctc
    14821 ctggcttctg aggaccgccc tgggcctggg agaatccctt ccccctcttc cctcgtgatc
    14881 tgcaactcca gtctttctag ccttaattaa gggatctgta gggcgcagta gtccagggtt
    14941 tccttgatga tgtcatactt atcctgtccc ttttttttcc acagctcgcg gttgaggaca
    15001 aactcttcgc ggtctttcca gtggggatcg acggtatcgt agagtcgagg ccgctctaga
    15061 actagttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
    15121 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
    15181 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
    15241 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
    15301 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
    15361 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
    15421 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
    15481 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
    15541 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gcctttgtct
    15601 caagaagaat ccaccctcat tgaaagagca acggctacaa tcaacagcat ccccatctct
    15661 gaagactaca gcgtcgccag cgcagctctc tctagcgacg gccgcatctt cactggtgtc
    15721 aatgtatatc attttactgg gggaccttgt gcagaactcg tggtgctggg cactgctgct
    15781 gctgcggcag ctggcaacct gacttgtatc gtcgcgatcg gaaatgagaa caggggcatc
    15841 ttgagcccct gcggacggtg ccgacaggtg cttctcgatc tgcatcctgg gatcaaagcc
    15901 atagtgaagg acagtgatgg acagccgacg gcagttggga ttcgtgaatt gctgccctct
    15961 ggttatgtgt gggagggcta agcacttcgt ggccgaggag caggactgac acgtgctacg
    16021 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga
    16081 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa
    16141 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
    16201 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
    16261 tcatgtctgc tagcatcgat accgtc
//
