LOCUS       sequence-310103-       15338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ROSA26 targetting vector with tamoxifen-inducible Cre recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15338)
  AUTHORS   Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB,
            Schar P, Schuermann D
  TITLE     Inducible TDG knockout models to study epigenetic regulation.
  JOURNAL   F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. 
            eCollection 2020.
  PUBMED    33082936
REFERENCE   2  (bases 1 to 15338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2020-09-9"; volume: "9:1112. doi"; pages: " 
            10.12688/f1000research.25637.2. eCollection 2020"
COMMENT     Sequence Label: pROSA26-CreERT2
FEATURES             Location/Qualifiers
     source          1..15338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     intron          655..1671
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1595..1617
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1679..1698
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1732..1741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1738..2766
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MANLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             2779..3714
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             2827..3720
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    3752..3976
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4030..8370
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        8420..8919
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8455..8478)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8852..8871
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8946..9518
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          9595..9660
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    9734..9958
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10020..10040)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10020..10038)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10059..10075)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10059..10075)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10072..10094)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10083..10099
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10107..10113,10114..10131,10132..10137))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10152..10173
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10290..10307)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10461..11049)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10541..10560)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11220..12011,12012..12080))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11843..11862
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12081..12185)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12212..12667
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12299..12318)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12509..12530
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12793..12815
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12807..12824
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12808..12824
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12834..12853
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        12834..12852
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    12871..13952
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(13971..14036,14037..14074)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(14187..14207)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14192..14521
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14372..14507
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14434..14453
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        14569..14616
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14635..15033
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    15191..15312
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15228..15247)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15282..15301
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggnnnnncg cgcgccaggc ggggcggggc ggggcgaggg gcggggcggg
      541 gcgaggcgga gaggtgcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
      601 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
      661 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
      721 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
      781 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
      841 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
      901 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
      961 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     1021 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     1081 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     1141 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     1201 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     1261 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     1321 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     1381 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     1441 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc cctctccagc ctcggggctg tccgcggggg
     1561 gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc
     1621 ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc
     1681 aacgtgctgg ttattgtgct gtctcatcat tttggcaaag aattctgagc cgccaccatg
     1741 gccaatttac tgaccgtaca ccaaaatttg cctgcattac cggtcgatgc aacgagtgat
     1801 gaggttcgca agaacctgat ggacatgttc agggatcgcc aggcgttttc tgagcatacc
     1861 tggaaaatgc ttctgtccgt ttgccggtcg tgggcggcat ggtgcaagtt gaataaccgg
     1921 aaatggtttc ccgcagaacc tgaagatgtt cgcgattatc ttctatatct tcaggcgcgc
     1981 ggtctggcag taaaaactat ccagcaacat ttgggccagc taaacatgct tcatcgtcgg
     2041 tccgggctgc cacgaccaag tgacagcaat gctgtttcac tggttatgcg gcggatccga
     2101 aaagaaaacg ttgatgccgg tgaacgtgca aaacaggctc tagcgttcga acgcactgat
     2161 ttcgaccagg ttcgttcact catggaaaat agcgatcgct gccaggatat acgtaatctg
     2221 gcatttctgg ggattgctta taacaccctg ttacgtatag ccgaaattgc caggatcagg
     2281 gttaaagata tctcacgtac tgacggtggg agaatgttaa tccatattgg cagaacgaaa
     2341 acgctggtta gcaccgcagg tgtagagaag gcacttagcc tgggggtaac taaactggtc
     2401 gagcgatgga tttccgtctc tggtgtagct gatgatccga ataactacct gttttgccgg
     2461 gtcagaaaaa atggtgttgc cgcgccatct gccaccagcc agctatcaac tcgcgccctg
     2521 gaagggattt ttgaagcaac tcatcgattg atttacggcg ctaaggatga ctctggtcag
     2581 agatacctgg cctggtctgg acacagtgcc cgtgtcggag ccgcgcgaga tatggcccgc
     2641 gctggagttt caataccgga gatcatgcaa gctggtggct ggaccaatgt aaatattgtc
     2701 atgaactata tccgtaacct ggatagtgaa acaggggcaa tggtgcgcct gctggaagat
     2761 ggcgatctcg agccatctgc tggagacatg agagctgcca acctttggcc aagcccgctc
     2821 atgatcaaac gctctaagaa gaacagcctg gccttgtccc tgacggccga ccagatggtc
     2881 agtgccttgt tggatgctga gccccccata ctctattccg agtatgatcc taccagaccc
     2941 ttcagtgaag cttcgatgat gggcttactg accaacctgg cagacaggga gctggttcac
     3001 atgatcaact gggcgaagag ggtgccaggc tttgtggatt tgaccctcca tgatcaggtc
     3061 caccttctag aatgtgcctg gctagagatc ctgatgattg gtctcgtctg gcgctccatg
     3121 gagcacccag tgaagctact gtttgctcct aacttgctct tggacaggaa ccagggaaaa
     3181 tgtgtagagg gcatggtgga gatcttcgac atgctgctgg ctacatcatc tcggttccgc
     3241 atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat ctattatttt gcttaattct
     3301 ggagtgtaca catttctgtc cagcaccctg aagtctctgg aagagaagga ccatatccac
     3361 cgagtcctgg acaagatcac agacactttg atccacctga tggccaaggc aggcctgacc
     3421 ctgcagcagc agcaccagcg gctggcccag ctcctcctca tcctctccca catcaggcac
     3481 atgagtaaca aaggcatgga gcatctgtac agcatgaagt gcaagaacgt ggtgcccctc
     3541 tatgacctgc tgctggaggc ggcggacgcc caccgcctac atgcgcccac tagccgtgga
     3601 ggggcatccg tggaggagac ggaccaaagc cacttggcca ctgcgggctc tacttcatcg
     3661 cattccttgc aaaagtatta catcacgggg gaggcagagg gtttccctgc cacagcttga
     3721 tagcggccgc gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     3781 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     3841 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     3901 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3961 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgat cctctagttg
     4021 gcgcgccggc tagaagatgg gcgggagtct tctgggcagg cttaaaggct aacctggtgt
     4081 gtgggcgttg tcctgcaggg gaattgaaca ggtgtaaaat tggagggaca agacttccca
     4141 cagattttcg gttttgtcgg gaagtttttt aataggggca aataaggaaa atgggaggat
     4201 aggtagtcat ctggggtttt atgcagcaaa actacaggtt attattgctt gtgatccgcc
     4261 tcggagtatt ttccatcgag gtagattaaa gacatgctca cccgagtttt atactctcct
     4321 gcttgagatc cttactacag tatgaaatta cagtgtcgcg agttagacta tgtaagcaga
     4381 attttaatca tttttaaaga gcccagtact tcatatccat ttctcccgct ccttctgcag
     4441 ccttatcaaa aggtatttta gaacactcat tttagcccca ttttcattta ttatactggc
     4501 ttatccaacc cctagacaga gcattggcat tttccctttc ctgatcttag aagtctgatg
     4561 actcatgaaa ccagacagat tagttacata caccacaaat cgaggctgta gctggggcct
     4621 caacactgca gttcttttat aactccttag tacacttttt gttgatcctt tgccttgatc
     4681 cttaattttc agtgtctatc acctctcccg tcaggtggtg ttccacattt gggcctattc
     4741 tcagtccagg gagttttaca acaatagatg tattgagaat ccaacctaaa gcttaacttt
     4801 ccactcccat gaatgcctct ctcctttttc tccatttata aactgagcta ttaaccatta
     4861 atggtttcca ggtggatgtc tcctccccca atattacctg atgtatctta catattgcca
     4921 ggctgatatt ttaagacatt aaaaggtata tttcattatt gagccacatg gtattgatta
     4981 ctgcttacta aaattttgtc attgtacaca tctgtaaaag gtggttcctt ttggaatgca
     5041 aagttcaggt gtttgttgtc tttcctgacc taaggtcttg tgagcttgta ttttttctat
     5101 ttaagcagtg ctttctcttg gactggcttg actcatggca ttctacacgt tattgctggt
     5161 ctaaatgtga ttttgccaag cttcttcagg acctataatt ttgcttgact tgtagccaaa
     5221 cacaagtaaa atgattaagc aacaaatgta tttgtgaagc ttggttttta ggttgttgtg
     5281 ttgtgtgtgc ttgtgctcta taataatact atccaggggc tggagaggtg gctcggagtt
     5341 caagagcaca gactgctctt ccagaagtcc tgagttcaat tcccagcaac cacatggtgg
     5401 ctcacaacca tctgtaatgg gatctgatgc cctcttctgg tgtgtctgaa gaccacaagt
     5461 gtattcacat taaataaata aatcctcctt cttcttcttt tttttttttt taaagagaat
     5521 actgtctcca gtagaattta ctgaagtaat gaaatacttt gtgtttgttc caatatggta
     5581 gccaataatc aaattactct ttaagcactg gaaatgttac caaggaacta atttttattt
     5641 gaagtgtaac tgtggacaga ggagccataa ctgcagactt gtgggataca gaagaccaat
     5701 gcagacttta atgtcttttc tcttacacta agcaataaag aaataaaaat tgaacttcta
     5761 gtatcctatt tgtttaaact gctagcttta cttaactttt gtgcttcatc tatacaaagc
     5821 tgaaagctaa gtctgcagcc attactaaac atgaaagcaa gtaatgataa ttttggattt
     5881 caaaaatgta gggccagagt ttagccagcc agtggtggtg cttgccttta tgcctttaat
     5941 cccagcactc tggaggcaga gacaggcaga tctctgagtt tgagcccagc ctggtctaca
     6001 catcaagttc tatctaggat agccaggaat acacacagaa accctgttgg ggaggggggc
     6061 tctgagattt cataaaatta taattgaagc attccctaat gagccactat ggatgtggct
     6121 aaatccgtct acctttctga tgagatttgg gtattatttt ttctgtctct gctgttggtt
     6181 gggtcttttg acactgtggg ctttctttaa agcctccttc ctgccatgtg gtctcttgtt
     6241 tgctactaac ttcccatggc ttaaatggca tggctttttg ccttctaagg gcagctgctg
     6301 agatttgcag cctgatttcc agggtggggt tgggaaatct ttcaaacact aaaattgtcc
     6361 tttaattttt tttttaaaaa atgggttata taataaacct cataaaatag ttatgaggag
     6421 tgaggtggac taatattaaa tgagtccctc ccctataaaa gagctattaa ggctttttgt
     6481 cttatactta actttttttt taaatgtggt atctttagaa ccaagggtct tagagtttta
     6541 gtatacagaa actgttgcat cgcttaatca gattttctag tttcaaatcc agagaatcca
     6601 aattcttcac agccaaagtc aaattaagaa tttctgactt ttaatgttaa tttgcttact
     6661 gtgaatataa aaatgatagc ttttcctgag gcagggtctc actatgtatc tctgcctgat
     6721 ctgcaacaag atatgtagac taaagttctg cctgcttttg tctcctgaat actaaggtta
     6781 aaatgtagta atacttttgg aacttgcagg tcagattctt ttatagggga cacactaagg
     6841 gagcttgggt gatagttggt aaaatgtgtt tcaagtgatg aaaacttgaa ttattatcac
     6901 cgcaacctac tttttaaaaa aaaaagccag gcctgttaga gcatgcttaa gggatcccta
     6961 ggacttgctg agcacacaag agtagttact tggcaggctc ctggtgagag catatttcaa
     7021 aaaacaaggc agacaaccaa gaaactacag ttaaggttac ctgtctttaa accatctgca
     7081 tatacacagg gatattaaaa tattccaaat aatatttcat tcaagttttc ccccatcaaa
     7141 ttgggacatg gatttctccg gtgaataggc agagttggaa actaaacaaa tgttggtttt
     7201 gtgatttgtg aaattgtttt caagtgatag ttaaagccca tgagatacag aacaaagctg
     7261 ctatttcgag gtctcttggt ttatactcag aagcacttct ttgggtttcc ctgcactatc
     7321 ctgatcatgt gctaggccta ccttaggctg attgttgttc aaataaactt aagtttcctg
     7381 tcaggtgatg tcatatgatt tcatatatca aggcaaaaca tgttatatat gttaaacatt
     7441 tgtacttaat gtgaaagtta ggtctttgtg ggtttgattt ttaattttca aaacctgagc
     7501 taaataagtc atttttacat gtcttacatt tggtggaatt gtataattgt ggtttgcagg
     7561 caagactctc tgacctagta accctaccta tagagcactt tgctgggtca caagtctagg
     7621 agtcaagcat ttcaccttga agttgagacg ttttgttagt gtatactagt ttatatgttg
     7681 gaggacatgt ttatccagaa gatattcagg actatttttg actgggctaa ggaattgatt
     7741 ctgattagca ctgttagtga gcattgagtg gcctttaggc ttgaattgga gtcacttgta
     7801 tatctcaaat aatgctggcc ttttttaaaa agcccttgtt ctttatcacc ctgttttcta
     7861 cataattttt gttcaaagaa atacttgttt ggatctcctt ttgacaacaa tagcatgttt
     7921 tcaagccata ttttttttcc tttttttttt tttttttggt ttttcgagac agggtttctc
     7981 tgtatagccc tggctgtcct ggaactcact ttgtagacca ggctggcctc gaactcagaa
     8041 atccgcctgc ctctgcctcc tgagtgccgg gattaaaggc gtgcaccacc acgcctggct
     8101 aagttggata ttttgttata taactataac caatactaac tccactgggt ggatttttaa
     8161 ttcagtcagt agtcttaagt ggtctttatt ggcccttcat taaaatctac tgttcactct
     8221 aacagaggct gttggtacta gtggcactta agcaacttcc tacggatata ctagcagatt
     8281 aagggtcagg gatagaaact agtctagcgt tttgtatacc taccagcttt atactacctt
     8341 gttctgatag aaatatttca ggacatctag cttatcgata ccgtcgacgg tatcgataag
     8401 cttgatatcg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     8461 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     8521 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     8581 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     8641 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     8701 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     8761 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     8821 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     8881 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcaggtc ctcgccatgg
     8941 atcctgatga tgttgttgat tcttctaaat cttttgtgat ggaaaacttt tcttcgtacc
     9001 acgggactaa acctggttat gtagattcca ttcaaaaagg tatacaaaag ccaaaatctg
     9061 gtacacaagg aaattatgac gatgattgga aagggtttta tagtaccgac aataaatacg
     9121 acgctgcggg atactctgta gataatgaaa acccgctctc tggaaaagct ggaggcgtgg
     9181 tcaaagtgac gtatccagga ctgacgaagg ttctcgcact aaaagtggat aatgccgaaa
     9241 ctattaagaa agagttaggt ttaagtctca ctgaaccgtt gatggagcaa gtcggaacgg
     9301 aagagtttat caaaaggttc ggtgatggtg cttcgcgtgt agtgctcagc cttcccttcg
     9361 ctgaggggag ttctagcgtt gaatatatta ataactggga acaggcgaaa gcgttaagcg
     9421 tagaacttga gattaatttt gaaacccgtg gaaaacgtgg ccaagatgcg atgtatgagt
     9481 atatggctca agcctgtgca ggaaatcgtg tcaggcgatc tctttgtgaa ggaaccttac
     9541 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     9601 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     9661 attccaacct atggaactga tgaatgggag cagtggtgga atgcagatcc tagagctcgc
     9721 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     9781 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     9841 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     9901 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     9961 tctgaggcgg aaagaaccag ctggggctcg agggggggcc cggtacccag cttttgttcc
    10021 ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga
    10081 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
    10141 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
    10201 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
    10261 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
    10321 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
    10381 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
    10441 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
    10501 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
    10561 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
    10621 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
    10681 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
    10741 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
    10801 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
    10861 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
    10921 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
    10981 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
    11041 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
    11101 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
    11161 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
    11221 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
    11281 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
    11341 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
    11401 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
    11461 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
    11521 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
    11581 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
    11641 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
    11701 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
    11761 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
    11821 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
    11881 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
    11941 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
    12001 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
    12061 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
    12121 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
    12181 ccgcgcacat ttccccgaaa agtgccacct gacgcgccct gtagcggcgc attaagcgcg
    12241 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
    12301 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
    12361 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
    12421 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
    12481 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
    12541 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
    12601 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
    12661 acaatttcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
    12721 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
    12781 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc gcgtaatacg
    12841 actcactata gggcgaattg gagctccacc gcggcaggcc ctccgagcgt ggtggagccg
    12901 ttctgtgaga cagccgggta cgagtcgtga cgctggaagg ggcaagcggg tggtgggcag
    12961 gaatgcggtc cgccctgcag caaccggagg gggagggaga agggagcgga aaagtctcca
    13021 ccggacgcgg ccatggctcg gggggggggg gcagcggagg agcgcttccg gccgacgtct
    13081 cgtcgctgat tggcttcttt tcctcccgcc gtgtgtgaaa acacaaatgg cgtgttttgg
    13141 ttggcgtaag gcgcctgtca gttaacggca gccggagtgc gcagccgccg gcagcctcgc
    13201 tctgcccact gggtggggcg ggaggtaggt ggggtgaggc gagctggacg tgcgggcgcg
    13261 gtcggcctct ggcggggcgg gggaggggag ggagggtcag cgaaagtagc tcgcgcgcga
    13321 gcggccgccc accctcccct tcctctgggg gagtcgtttt acccgccgcc ggccgggcct
    13381 cgtcgtctga ttggctctcg gggcccagaa aactggccct tgccattggc tcgtgttcgt
    13441 gcaagttgag tccatccgcc ggccagcggg ggcggcgagg aggcgctccc aggttccggc
    13501 cctcccctcg gccccgcgcc gcagagtctg gccgcgcgcc cctgcgcaac gtggcaggaa
    13561 gcgcgcgctg ggggcgggga cgggcagtag ggctgagcgg ctgcggggcg ggtgcaagca
    13621 cgtttccgac ttgagttgcc tcaagagggg cgtgctgagc cagacctcca tcgcgcactc
    13681 cggggagtgg agggaaggag cgagggctca gttgggctgt tttggaggca ggaagcactt
    13741 gctctcccaa agtcgctctg agttgttatc agtaagggag ctgcagtgga gtaggcgggg
    13801 agaaggccgc acccttctcc ggagggggga ggggagtgtt gcaatacctt tctgggagtt
    13861 ctctgctgcc tcctggcttc tgaggaccgc cctgggcctg ggagaatccc ttccccctct
    13921 tccctcgtga tctgcaactc cagtctttct agccttaatt aagggatctg tagggcgcag
    13981 tagtccaggg tttccttgat gatgtcatac ttatcctgtc cctttttttt ccacagctcg
    14041 cggttgagga caaactcttc gcggtctttc cagtggggat cgacggtatc gtagagtcga
    14101 ggccgctcta gaactagttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
    14161 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
    14221 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
    14281 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    14341 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
    14401 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
    14461 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
    14521 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
    14581 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
    14641 aagcctttgt ctcaagaaga atccaccctc attgaaagag caacggctac aatcaacagc
    14701 atccccatct ctgaagacta cagcgtcgcc agcgcagctc tctctagcga cggccgcatc
    14761 ttcactggtg tcaatgtata tcattttact gggggacctt gtgcagaact cgtggtgctg
    14821 ggcactgctg ctgctgcggc agctggcaac ctgacttgta tcgtcgcgat cggaaatgag
    14881 aacaggggca tcttgagccc ctgcggacgg tgccgacagg tgcttctcga tctgcatcct
    14941 gggatcaaag ccatagtgaa ggacagtgat ggacagccga cggcagttgg gattcgtgaa
    15001 ttgctgccct ctggttatgt gtgggagggc taagcacttc gtggccgagg agcaggactg
    15061 acacgtgcta cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat
    15121 cgttttccgg gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt
    15181 cgcccacccc aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
    15241 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
    15301 caatgtatct tatcatgtct gctagcatcg ataccgtc
//
