LOCUS       sequence-310104-       15590 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ROSA26 mouse targeting vector with tamoxifen-inducible Flp 
            recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15590)
  AUTHORS   Schwarz SD, Grundbacher E, Hrovat AM, Xu J, Kusnierczyk A, Vagbo CB,
            Schar P, Schuermann D
  TITLE     Inducible TDG knockout models to study epigenetic regulation.
  JOURNAL   F1000Res. 2020 Sep 9;9:1112. doi: 10.12688/f1000research.25637.2. 
            eCollection 2020.
  PUBMED    33082936
REFERENCE   2  (bases 1 to 15590)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "F1000Res.";
            date: "2020-09-9"; volume: "9:1112. doi"; pages: " 
            10.12688/f1000research.25637.2. eCollection 2020"
COMMENT     Sequence Label: pROSA26-FlpERT2
FEATURES             Location/Qualifiers
     source          1..15590
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..659
                     /label=chicken beta-actin promoter
     intron          660..1676
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1600..1622
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1684..1703
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             1743..3011
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     CDS             3030..3965
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3078..3971
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPVKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATA"
     polyA_signal    4003..4227
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    4281..8621
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        8671..9170
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(8706..8729)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9103..9122
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             9197..9769
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          9846..9911
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    9985..10209
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(10271..10291)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10271..10289)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10310..10326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10310..10326)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10323..10345)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10334..10350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10358..10364,10365..10382,10383..10388))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10403..10424
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10541..10558)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10712..11300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10792..10811)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11471..12262,12263..12331))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12094..12113
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12332..12436)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      12463..12918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12550..12569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12760..12781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     13044..13066
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13058..13075
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13059..13075
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13085..13104
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        13085..13103
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    13122..14204
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    join(14223..14288,14289..14326)
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     primer_bind     complement(14439..14459)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        14444..14773
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      14624..14759
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     14686..14705
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        14821..14868
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             14887..15285
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    15443..15564
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15480..15499)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     15534..15553
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      421 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      481 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      541 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      601 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      661 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      721 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      781 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      841 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gctccgcgct gcccggcggc tgtgagcgct
      961 gcgggcgcgg cgcggggctt tgtgcgctcc gcagtgtgcg cgaggggagc gcggccgggg
     1021 gcggtgcccc gcggtgcggg gggggctgcg aggggaacaa aggctgcgtg cggggtgtgt
     1081 gcgtgggggg gtgagcaggg ggtgtgggcg cgtcggtcgg gctgcaaccc cccctgcacc
     1141 cccctccccg agttgctgag cacggcccgg cttcgggtgc ggggctccgt acggggcgtg
     1201 gcgcggggct cgccgtgccg ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg
     1261 ggccgcctcg ggccggggag ggctcggggg aggggcgcgg cggcccccgg agcgccggcg
     1321 gctgtcgagg cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc
     1381 agggacttcc tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc
     1441 cctctagcgg gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg
     1501 gccttcgtgc gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc
     1561 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     1621 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     1681 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattc tgagccgcca
     1741 ccatgagcca atttgatata ttatgtaaaa caccacctaa ggtcctggtt cgtcagtttg
     1801 tggaaaggtt tgaaagacct tcaggggaaa aaatagcatc atgtgctgct gaactaacct
     1861 atttatgttg gatgattact cataacggaa cagcaatcaa gagagccaca ttcatgagct
     1921 ataatactat cataagcaat tcgctgagtt tcgatattgt caacaaatca ctccagttta
     1981 aatacaagac gcaaaaagca acaattctgg aagcctcatt aaagaaatta attcctgctt
     2041 gggaatttac aattattcct tacaatggac aaaaacatca atctgatatc actgatattg
     2101 taagtagttt gcaattacag ttcgaatcat cggaagaagc agataaggga aatagccaca
     2161 gtaaaaaaat gcttaaagca cttctaagtg agggtgaaag catctgggag atcactgaga
     2221 aaatactaaa ttcgtttgag tatacctcga gatttacaaa aacaaaaact ttataccaat
     2281 tcctcttcct agctactttc atcaattgtg gaagattcag cgatattaag aacgttgatc
     2341 cgaaatcatt taaattagtc caaaataagt atctgggagt aataatccag tgtttagtga
     2401 cagagacaaa gacaagcgtt agtaggcaca tatacttctt tagcgcaagg ggtaggatcg
     2461 atccacttgt atatttggat gaatttttga ggaactctga accagtccta aaacgagtaa
     2521 ataggaccgg caattcttca agcaacaaac aggaatacca attattaaaa gataacttag
     2581 tcagatcgta caacaaggct ttgaagaaaa atgcgcctta tccaatcttt gctataaaga
     2641 atggcccaaa atctcacatt ggaagacatt tgatgacctc atttctgtca atgaagggcc
     2701 taacggagtt gactaatgtt gtgggaaatt ggagcgataa gcgtgcttct gccgtggcca
     2761 ggacaacgta tactcatcag ataacagcaa tacctgatca ctacttcgca ctagtttctc
     2821 ggtactatgc atatgatcca atatcaaagg aaatgatagc attgaaggat gagactaatc
     2881 caattgagga gtggcagcat atagaacagc taaagggtag tgctgaagga agcatacgat
     2941 accccgcatg gaatgggata atatcacagg aggtactaga ctacctttca tcctacataa
     3001 atagacgcat atgcgtacgc ggatcctctg ctggagacat gagagctgcc aacctttggc
     3061 caagcccgct catgatcaaa cgctctaaga agaacagcct ggccttgtcc ctgacggccg
     3121 accagatggt cagtgccttg ttggatgctg agccccccat actctattcc gagtatgatc
     3181 ctaccagacc cttcagtgaa gcttcgatga tgggcttact gaccaacctg gcagacaggg
     3241 agctggttca catgatcaac tgggcgaaga gggtgccagg ctttgtggat ttgaccctcc
     3301 atgatcaggt ccaccttcta gaatgtgcct ggctagagat cctgatgatt ggtctcgtct
     3361 ggcgctccat ggagcaccca gtgaagctac tgtttgctcc taacttgctc ttggacagga
     3421 accagggaaa atgtgtagag ggcatggtgg agatcttcga catgctgctg gctacatcat
     3481 ctcggttccg catgatgaat ctgcagggag aggagtttgt gtgcctcaaa tctattattt
     3541 tgcttaattc tggagtgtac acatttctgt ccagcaccct gaagtctctg gaagagaagg
     3601 accatatcca ccgagtcctg gacaagatca cagacacttt gatccacctg atggccaagg
     3661 caggcctgac cctgcagcag cagcaccagc ggctggccca gctcctcctc atcctctccc
     3721 acatcaggca catgagtaac aaaggcatgg agcatctgta cagcatgaag tgcaagaacg
     3781 tggtgcccct ctatgacctg ctgctggagg cggcggacgc ccaccgccta catgcgccca
     3841 ctagccgtgg aggggcatcc gtggaggaga cggaccaaag ccacttggcc actgcgggct
     3901 ctacttcatc gcattccttg caaaagtatt acatcacggg ggaggcagag ggtttccctg
     3961 ccacagcttg atagcggccg cgagctcgct gatcagcctc gactgtgcct tctagttgcc
     4021 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4081 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4141 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4201 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcga
     4261 tcctctagtt ggcgcgccgg ctagaagatg ggcgggagtc ttctgggcag gcttaaaggc
     4321 taacctggtg tgtgggcgtt gtcctgcagg ggaattgaac aggtgtaaaa ttggagggac
     4381 aagacttccc acagattttc ggttttgtcg ggaagttttt taataggggc aaataaggaa
     4441 aatgggagga taggtagtca tctggggttt tatgcagcaa aactacaggt tattattgct
     4501 tgtgatccgc ctcggagtat tttccatcga ggtagattaa agacatgctc acccgagttt
     4561 tatactctcc tgcttgagat ccttactaca gtatgaaatt acagtgtcgc gagttagact
     4621 atgtaagcag aattttaatc atttttaaag agcccagtac ttcatatcca tttctcccgc
     4681 tccttctgca gccttatcaa aaggtatttt agaacactca ttttagcccc attttcattt
     4741 attatactgg cttatccaac ccctagacag agcattggca ttttcccttt cctgatctta
     4801 gaagtctgat gactcatgaa accagacaga ttagttacat acaccacaaa tcgaggctgt
     4861 agctggggcc tcaacactgc agttctttta taactcctta gtacactttt tgttgatcct
     4921 ttgccttgat ccttaatttt cagtgtctat cacctctccc gtcaggtggt gttccacatt
     4981 tgggcctatt ctcagtccag ggagttttac aacaatagat gtattgagaa tccaacctaa
     5041 agcttaactt tccactccca tgaatgcctc tctccttttt ctccatttat aaactgagct
     5101 attaaccatt aatggtttcc aggtggatgt ctcctccccc aatattacct gatgtatctt
     5161 acatattgcc aggctgatat tttaagacat taaaaggtat atttcattat tgagccacat
     5221 ggtattgatt actgcttact aaaattttgt cattgtacac atctgtaaaa ggtggttcct
     5281 tttggaatgc aaagttcagg tgtttgttgt ctttcctgac ctaaggtctt gtgagcttgt
     5341 attttttcta tttaagcagt gctttctctt ggactggctt gactcatggc attctacacg
     5401 ttattgctgg tctaaatgtg attttgccaa gcttcttcag gacctataat tttgcttgac
     5461 ttgtagccaa acacaagtaa aatgattaag caacaaatgt atttgtgaag cttggttttt
     5521 aggttgttgt gttgtgtgtg cttgtgctct ataataatac tatccagggg ctggagaggt
     5581 ggctcggagt tcaagagcac agactgctct tccagaagtc ctgagttcaa ttcccagcaa
     5641 ccacatggtg gctcacaacc atctgtaatg ggatctgatg ccctcttctg gtgtgtctga
     5701 agaccacaag tgtattcaca ttaaataaat aaatcctcct tcttcttctt tttttttttt
     5761 ttaaagagaa tactgtctcc agtagaattt actgaagtaa tgaaatactt tgtgtttgtt
     5821 ccaatatggt agccaataat caaattactc tttaagcact ggaaatgtta ccaaggaact
     5881 aatttttatt tgaagtgtaa ctgtggacag aggagccata actgcagact tgtgggatac
     5941 agaagaccaa tgcagacttt aatgtctttt ctcttacact aagcaataaa gaaataaaaa
     6001 ttgaacttct agtatcctat ttgtttaaac tgctagcttt acttaacttt tgtgcttcat
     6061 ctatacaaag ctgaaagcta agtctgcagc cattactaaa catgaaagca agtaatgata
     6121 attttggatt tcaaaaatgt agggccagag tttagccagc cagtggtggt gcttgccttt
     6181 atgcctttaa tcccagcact ctggaggcag agacaggcag atctctgagt ttgagcccag
     6241 cctggtctac acatcaagtt ctatctagga tagccaggaa tacacacaga aaccctgttg
     6301 gggagggggg ctctgagatt tcataaaatt ataattgaag cattccctaa tgagccacta
     6361 tggatgtggc taaatccgtc tacctttctg atgagatttg ggtattattt tttctgtctc
     6421 tgctgttggt tgggtctttt gacactgtgg gctttcttta aagcctcctt cctgccatgt
     6481 ggtctcttgt ttgctactaa cttcccatgg cttaaatggc atggcttttt gccttctaag
     6541 ggcagctgct gagatttgca gcctgatttc cagggtgggg ttgggaaatc tttcaaacac
     6601 taaaattgtc ctttaatttt ttttttaaaa aatgggttat ataataaacc tcataaaata
     6661 gttatgagga gtgaggtgga ctaatattaa atgagtccct cccctataaa agagctatta
     6721 aggctttttg tcttatactt aacttttttt ttaaatgtgg tatctttaga accaagggtc
     6781 ttagagtttt agtatacaga aactgttgca tcgcttaatc agattttcta gtttcaaatc
     6841 cagagaatcc aaattcttca cagccaaagt caaattaaga atttctgact tttaatgtta
     6901 atttgcttac tgtgaatata aaaatgatag cttttcctga ggcagggtct cactatgtat
     6961 ctctgcctga tctgcaacaa gatatgtaga ctaaagttct gcctgctttt gtctcctgaa
     7021 tactaaggtt aaaatgtagt aatacttttg gaacttgcag gtcagattct tttatagggg
     7081 acacactaag ggagcttggg tgatagttgg taaaatgtgt ttcaagtgat gaaaacttga
     7141 attattatca ccgcaaccta ctttttaaaa aaaaaagcca ggcctgttag agcatgctta
     7201 agggatccct aggacttgct gagcacacaa gagtagttac ttggcaggct cctggtgaga
     7261 gcatatttca aaaaacaagg cagacaacca agaaactaca gttaaggtta cctgtcttta
     7321 aaccatctgc atatacacag ggatattaaa atattccaaa taatatttca ttcaagtttt
     7381 cccccatcaa attgggacat ggatttctcc ggtgaatagg cagagttgga aactaaacaa
     7441 atgttggttt tgtgatttgt gaaattgttt tcaagtgata gttaaagccc atgagataca
     7501 gaacaaagct gctatttcga ggtctcttgg tttatactca gaagcacttc tttgggtttc
     7561 cctgcactat cctgatcatg tgctaggcct accttaggct gattgttgtt caaataaact
     7621 taagtttcct gtcaggtgat gtcatatgat ttcatatatc aaggcaaaac atgttatata
     7681 tgttaaacat ttgtacttaa tgtgaaagtt aggtctttgt gggtttgatt tttaattttc
     7741 aaaacctgag ctaaataagt catttttaca tgtcttacat ttggtggaat tgtataattg
     7801 tggtttgcag gcaagactct ctgacctagt aaccctacct atagagcact ttgctgggtc
     7861 acaagtctag gagtcaagca tttcaccttg aagttgagac gttttgttag tgtatactag
     7921 tttatatgtt ggaggacatg tttatccaga agatattcag gactattttt gactgggcta
     7981 aggaattgat tctgattagc actgttagtg agcattgagt ggcctttagg cttgaattgg
     8041 agtcacttgt atatctcaaa taatgctggc cttttttaaa aagcccttgt tctttatcac
     8101 cctgttttct acataatttt tgttcaaaga aatacttgtt tggatctcct tttgacaaca
     8161 atagcatgtt ttcaagccat attttttttc cttttttttt ttttttttgg tttttcgaga
     8221 cagggtttct ctgtatagcc ctggctgtcc tggaactcac tttgtagacc aggctggcct
     8281 cgaactcaga aatccgcctg cctctgcctc ctgagtgccg ggattaaagg cgtgcaccac
     8341 cacgcctggc taagttggat attttgttat ataactataa ccaatactaa ctccactggg
     8401 tggattttta attcagtcag tagtcttaag tggtctttat tggcccttca ttaaaatcta
     8461 ctgttcactc taacagaggc tgttggtact agtggcactt aagcaacttc ctacggatat
     8521 actagcagat taagggtcag ggatagaaac tagtctagcg ttttgtatac ctaccagctt
     8581 tatactacct tgttctgata gaaatatttc aggacatcta gcttatcgat accgtcgacg
     8641 gtatcgataa gcttgatatc gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     8701 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     8761 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     8821 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     8881 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     8941 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     9001 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     9061 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     9121 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcaggt
     9181 cctcgccatg gatcctgatg atgttgttga ttcttctaaa tcttttgtga tggaaaactt
     9241 ttcttcgtac cacgggacta aacctggtta tgtagattcc attcaaaaag gtatacaaaa
     9301 gccaaaatct ggtacacaag gaaattatga cgatgattgg aaagggtttt atagtaccga
     9361 caataaatac gacgctgcgg gatactctgt agataatgaa aacccgctct ctggaaaagc
     9421 tggaggcgtg gtcaaagtga cgtatccagg actgacgaag gttctcgcac taaaagtgga
     9481 taatgccgaa actattaaga aagagttagg tttaagtctc actgaaccgt tgatggagca
     9541 agtcggaacg gaagagttta tcaaaaggtt cggtgatggt gcttcgcgtg tagtgctcag
     9601 ccttcccttc gctgagggga gttctagcgt tgaatatatt aataactggg aacaggcgaa
     9661 agcgttaagc gtagaacttg agattaattt tgaaacccgt ggaaaacgtg gccaagatgc
     9721 gatgtatgag tatatggctc aagcctgtgc aggaaatcgt gtcaggcgat ctctttgtga
     9781 aggaacctta cttctgtggt gtgacataat tggacaaact acctacagag atttaaagct
     9841 ctaaggtaaa tataaaattt ttaagtgtat aatgtgttaa actactgatt ctaattgttt
     9901 gtgtatttta gattccaacc tatggaactg atgaatggga gcagtggtgg aatgcagatc
     9961 ctagagctcg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
    10021 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
    10081 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
    10141 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
    10201 gctctatggc ttctgaggcg gaaagaacca gctggggctc gagggggggc ccggtaccca
    10261 gcttttgttc cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt
    10321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
    10381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
    10441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
    10501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
    10561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
    10621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
    10681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
    10741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
    10801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
    10861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
    10921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
    10981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
    11041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
    11101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
    11161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
    11221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
    11281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
    11341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
    11401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
    11461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
    11521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
    11581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
    11641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
    11701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
    11761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
    11821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
    11881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
    11941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
    12001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
    12061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
    12121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
    12181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
    12241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
    12301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
    12361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
    12421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg
    12481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
    12541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
    12601 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
    12661 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
    12721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
    12781 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
    12841 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
    12901 tattaacgct tacaatttcc attcgccatt caggctgcgc aactgttggg aagggcgatc
    12961 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
    13021 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg
    13081 cgcgtaatac gactcactat agggcgaatt ggagctccac cgcggcaggc cctccgagcg
    13141 tggtggagcc gttctgtgag acagccgggt acgagtcgtg acgctggaag gggcaagcgg
    13201 gtggtgggca ggaatgcggt ccgccctgca gcaaccggag ggggagggag aagggagcgg
    13261 aaaagtctcc accggacgcg gccatggctc gggggggggg gggcagcgga ggagcgcttc
    13321 cggccgacgt ctcgtcgctg attggcttct tttcctcccg ccgtgtgtga aaacacaaat
    13381 ggcgtgtttt ggttggcgta aggcgcctgt cagttaacgg cagccggagt gcgcagccgc
    13441 cggcagcctc gctctgccca ctgggtgggg cgggaggtag gtggggtgag gcgagctgga
    13501 cgtgcgggcg cggtcggcct ctggcggggc gggggagggg agggagggtc agcgaaagta
    13561 gctcgcgcgc gagcggccgc ccaccctccc cttcctctgg gggagtcgtt ttacccgccg
    13621 ccggccgggc ctcgtcgtct gattggctct cggggcccag aaaactggcc cttgccattg
    13681 gctcgtgttc gtgcaagttg agtccatccg ccggccagcg ggggcggcga ggaggcgctc
    13741 ccaggttccg gccctcccct cggccccgcg ccgcagagtc tggccgcgcg cccctgcgca
    13801 acgtggcagg aagcgcgcgc tgggggcggg gacgggcagt agggctgagc ggctgcgggg
    13861 cgggtgcaag cacgtttccg acttgagttg cctcaagagg ggcgtgctga gccagacctc
    13921 catcgcgcac tccggggagt ggagggaagg agcgagggct cagttgggct gttttggagg
    13981 caggaagcac ttgctctccc aaagtcgctc tgagttgtta tcagtaaggg agctgcagtg
    14041 gagtaggcgg ggagaaggcc gcacccttct ccggaggggg gaggggagtg ttgcaatacc
    14101 tttctgggag ttctctgctg cctcctggct tctgaggacc gccctgggcc tgggagaatc
    14161 ccttccccct cttccctcgt gatctgcaac tccagtcttt ctagccttaa ttaagggatc
    14221 tgtagggcgc agtagtccag ggtttccttg atgatgtcat acttatcctg tccctttttt
    14281 ttccacagct cgcggttgag gacaaactct tcgcggtctt tccagtgggg atcgacggta
    14341 tcgtagagtc gaggccgctc tagaactagt tgccgatttc ggcctattgg ttaaaaaatg
    14401 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg
    14461 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
    14521 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
    14581 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
    14641 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    14701 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    14761 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcagcacgt
    14821 gttgacaatt aatcatcggc atagtatatc ggcatagtat aatacgacaa ggtgaggaac
    14881 taaaccatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
    14941 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
    15001 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa
    15061 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
    15121 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc
    15181 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
    15241 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctaagcact tcgtggccga
    15301 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    15361 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    15421 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    15481 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    15541 gtccaaactc atcaatgtat cttatcatgt ctgctagcat cgataccgtc
//
