LOCUS       sequence-310118-       15870 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CRISPRa proof-of-concept test target with fluorescent reporter. 
            PelcA is fused to mCherry, with low basal expression in Aspergillus 
            nidulans. Fungal vector with AMA1 and pyrG selection marker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15870)
  AUTHORS   Roux I, Woodcraft C, Hu J, Wolters R, Gilchrist CLM, Chooi YH
  TITLE     CRISPR-mediated activation of biosynthetic gene clusters for 
            bioactive molecule discovery in filamentous fungi.
  JOURNAL   ACS Synth Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197.
  PUBMED    32526136
REFERENCE   2  (bases 1 to 15870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2020 Jun 11. doi: 10.1021/acssynbio.0c00197."
COMMENT     Sequence Label: pCRI009-pKW20088-PelcA-mCherry-TtrpC-NotI-PacI
FEATURES             Location/Qualifiers
     source          1..15870
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     2802..2823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             3982..4692
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKGGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4127..4145)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4395..4414
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             join(7954..8022,8023..8814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8172..8191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8985..9573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9474..9493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9727..9744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9861..9882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9897..9902,9903..9920,9921..9927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9935..9951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9940..9962
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9959..9975
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9959..9975
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      9992..11334
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10955..11002)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11375..11393
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(11431..11453)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     11553..11572
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        11596..11811
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             11812..12615
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     primer_bind     complement(12897..12914)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(12897..12913)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12906..12928)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(13213..13232)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     rep_origin      join(13254..15870,1..2632)
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
ORIGIN
        1 tgcagatttt gacctagtta acaaggcaca tcatgctatc acttccaaca ctcttgctga
       61 gcgcgttctg ccttgccttt ctggttctac gaagtatcca gtcccgcctg caacgccgca
      121 gaaacgccaa aaggtggggt tgtcaaccgc cagctagagc tccatcggga ttctttggca
      181 ttccgccgtt cctcggtggt cgcagataac tccctaaatt ccctgagcta actctaagtc
      241 gaccatgccg tttatggtta gcgcctccca aaaaggaatg gccgacttag agttacctct
      301 tgaccgactt tttctttcct cccccttaca tttcgttacc acaacacatt cctatatcca
      361 aactccaggt acataactag tcgaaatctc ttttaaatct agtcaagaac tagatactta
      421 accttcatac tgcttagaga tagcctttga agctcttatt tcgctctttg tctcactctc
      481 accttcctcc tcctcctcca atcctttttg ctcgggacta gtccaagact agtcaacgat
      541 tagtatgccg agcattcgcg ataacgatct ccgaaagtcc ccagagtact gtcactatct
      601 cgaggcagtt aaggacgggg agcttacgct gccggatttc aagatagtaa gccgacccga
      661 ctagctttaa gcctagttac tgactagtcc acaggacgac aatggcgtgc ctgatatcca
      721 tccatatgaa gtctactgcc gagtgaaggg atgcctcaag cgtacagtga gtctactgct
      781 atactagttt ctgactagtt attaactagt tgacaggttc cctctgccaa cagaaacata
      841 ttggtcaagc acttgaagga caagaactcc cacggcatgg agtttacatt gcacaatgga
      901 cctcccacta tgaaggaact gatggaggcc aaaggcaagt cctatctaga ttacttggtg
      961 actagtccct gactagtcaa gtagcatggt atgaaggctt gtttgaaggc actgttctcc
     1021 caaccccgac tcctaccaag aagcgcaagc gagctgcgta agtttctgtg agtctaacta
     1081 gtgtattagc taatatatag cagcaccaag tccaaggacc acaatactaa gggtgtcgag
     1141 aattcgtgag tttcttctcc catttcaact agtccttgac tagtcactaa ctactttgca
     1201 gcaacgaggg tgaagctgga aatgatcagg acaatggcga gggcccgtaa gtacagccat
     1261 tcaatgcaga ctagttgcta actaatctgt gactagttca agtggtccgt acgccgtgca
     1321 tacccctgtg actggtagga atttgagcaa gcctgtcttg ccgcgcgatg agaaaggaaa
     1381 ggcaagttac attcagcccc gtacctagga tcagtgctaa tttataacct ctagccactc
     1441 tttatgcaga tccgccgtga gggtagcaag gcagctaaat cagctggtga gaaaggaacc
     1501 ataccctgca agacctgtcg caacgcaaag ggcaaaggta agctatccaa gctagtttgg
     1561 gactagattc taactagtct cagcaccgtg tggttcaaag ccatattgcg agttttggcg
     1621 ctttttctca tcgattgacg aggcaaaggg agcgagtatg caacctcgta agtcagactc
     1681 agacaaagac caactagtta ttgaccagtc ttctagaagg ctctgttgtg gatcttgagg
     1741 ccctggagag ttcctccaac aatccggaga caagcaagtc ctcgtcggac tagtcactaa
     1801 ctagactcta actagttgca gacatggata atgcaaaaga gacaagcaat gaagaaagtg
     1861 gtaagacatt tctcctttgt ggttctggac tagtctttga ctagtcacag tcttaaacaa
     1921 ggaaaatgag catgaaaatg aggaggaaaa ggctgctgag cccgaggaag tgcagggtga
     1981 tggcagacat ggtaggttaa taccttgtta gttattgcta gtcactgact agtcaataac
     2041 tagtctctga acaccttgca atcactccgt ttgcgcagct gaacagtggt gaggataata
     2101 gtagtaagtt attctagctt cagagttata ggagactaga tactaactag tattagttgc
     2161 aactaacctg gatctcagag actttggcct caatctagaa tctatctagt tgtcaactag
     2221 actgtggtat cattgtcttt tattttccta gtcctggaac tagcttctaa ctagtctccc
     2281 taatatgtgg ctgtcttgtt tttttttttt gtttccctac ccggatatct agtccccttc
     2341 taggttctgt taacctctcg ggctctgatt tagtttaacg caaacctgag attagtttct
     2401 aactagtctc taggttttct atccaccttt aattgtaata ataaatacaa gcaacgttta
     2461 tacgtcaaaa gcatttataa acttttaccc taaagtagct tgcttgtgtg tttagtttat
     2521 aattagtctc ttattaattt gatgtaggta agcccgccac aaatatatat ttttaacaag
     2581 ataccgtgga aaaacttcgt gctatcacaa aacagtatac aaaaaataag ctggctttcc
     2641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     2701 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     2761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     2821 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     2881 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     2941 aatggggtga cgatgagccg ctcttgcatc tttgtttgta ttatactgtc tttcttgtta
     3001 cacataatta ttctagaatg ccccaccgtt acatacggga cacagccatt tacatatgca
     3061 tgtggattac gagctaacga gttcattcaa atctcagaac tatcacataa tcatcattcc
     3121 cctatcgtca aagaccgtaa gacaaatccg gttcatgcac tgaacccatt cgggtagtga
     3181 gtcatttact cagcacactc gcgctgacgc tcgtcgaaca ccttcaatgc ctcctcggca
     3241 gccttgacac cactgagaac catggcaccg aaggtagggc ccatgcggtt aaagccatca
     3301 atttcagaca gctccatacc gccgattatc aagcccttag taacctcgcg ggtgttcttg
     3361 acgatggcat cctgccgatg tacagtgctg aggcacgtca tactatgtat tagtagagga
     3421 tctagttgcc aatatcacgg agagcggaca gcttggtgtt gacaagcgcg taagctttac
     3481 catgatgtta gtgatgatct cgatctaccc agatgcatgg cttgagacac gtacccatgg
     3541 tggtcttggg tagctggaat ggggccagcg gccagtgccg gctcaccaat cgccagtgga
     3601 tgaagctcga tcgcgaccag ctagatcgat gctcagtact atgatgagtg gaatgaaagc
     3661 cttacaacca ggttttcggc caaaattggc cgcagttgcc agtacagtgt tgagtaaact
     3721 tcgtcatgga gcgtttcgat ggtggtgcta gcttctcgat catcagtgcc atgatacttt
     3781 cacccgtgcc aaagtgacga ccagctccat ataatatgac ggtatggacg gtgctgtcta
     3841 tccagattca tctctgatca gcgcaatcgt ttcaacatcc ttgtgtgaat attcaccaga
     3901 cagtcgttaa taccctttta gtcacccttc gaagctatta ttgtctcagc cactacgtca
     3961 ctcaacttgc gttatctcaa tatggtgagc aagggcgagg aggataacat ggccatcatc
     4021 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4081 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4141 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4201 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4261 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4321 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4381 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4441 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4501 ggcggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4561 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     4621 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     4681 ctgtacaagt aatgggcgac gacgacgttc gagggggggc ccggtaccgc cccgtccggt
     4741 cctgcccgtc accgagatcc acttaacgtt actgaaatca tcaaacagct tgacgaatct
     4801 ggatataaga tcgttggtgt cgatgtcagc tccggagttg agacaaatgg tgttcaggat
     4861 ctcgataaga tacgttcatt tgtccaagca gcaaagagtg ccttctagtg atttaatagc
     4921 tccatgtcaa caagaataaa acgcgtttcg ggtttacctc ttccagatac agctcatctg
     4981 caatgcatga cgttaattaa cgaatagcgg ccgcccagaa aattacgggt atgtcctcca
     5041 ctgtgttgaa gtcagaaaaa caatcgataa tttgaagctc attaaaatgc cagttccgtc
     5101 gtcgccgttg ccctaggagc tattccagtg cttagcttca ttcatggatg tatcgtgact
     5161 ggccttcgga aggatgccaa ggtcccctat ccgcacagct atgcgaccgt tgagcaatgc
     5221 aagtcaaatg tgtgcatacc tggtctttgg tttggtacac cgctatgtga agattattaa
     5281 tgctgaagtc atgatacagg ccaaagccga gcaatttaac tgcgcgcagc gggcccacgc
     5341 taactacctc gagaatatgc ctcaaacaat gctcttcacc ctcttcgcgg gtctgaaata
     5401 ccctcacctg gcaacagcaa ttggcgcttc atggctgttt ttccgatctc tctacttgta
     5461 cggctatgtg tactcgggta agccacaagg caagggcaga ttgctgggag gtttcttctg
     5521 gttttctcaa ggcgctctgt gggctctgag tgtgtttggt gttgccaaag acatgatctc
     5581 ttactgagag ttattctgtg tctgacgaaa tatgttgtgt atatatatat atgtacgtta
     5641 aaagttccgt ggagttacca gtgattgacc aatgttttat cttctacagt tctgcctgtc
     5701 taccccattc tagctgtacc tgactacaga gtagtttaat tgtggttgac cccacagtcg
     5761 gaggcggagg aatacagcac cgatgtggcc tgtctccatc cagattggca cgcaattttt
     5821 acacgcggaa aagatcgaga tagagtacga ctttaaattt agtccccggc ggcttctatt
     5881 ttagaatatt tgagatttga ttctcaagca attgatttgg ttgggtcacc ctcaattgga
     5941 taatatacct cattgctcgg ctacttcaac tcatcaatca ccgtcatacc ccgcatataa
     6001 ccctccattc ccacgatgtc gtccaagtcg caattgactt acggtgctcg agccagcaag
     6061 caccccaatc ctctggcaaa gagacttttt gagattgccg aagcaaagaa gacaaacgtt
     6121 accgtctctg ctgatgtgac gacaacccga gaactcctgg acctcgctga ccgtacggaa
     6181 gctgttggat ccaatacata tgccgtctag caatggacta atcaactttt gatgatacag
     6241 gtctcggtcc ctacatcgcc gtcatcaaga cacacatcga catcctcacc gatttcagcg
     6301 tcgacactat caatggcctg aatgtgctgg ctcaaaagca caactttttg atcttcgagg
     6361 accgcaaatt catcgacatc ggcaataccg tccagaagca ataccacggc ggtgctctga
     6421 ggatctccga atgggcccac attatcaact gcagcgttct ccctggcgag ggcatcgtcg
     6481 aggctctggc ccagaccgca tctgcgcaag acttccccta tggtcctgag agaggactgt
     6541 tggtcctggc agagatgacc tccaaaggat cgctggctac gggcgagtat accaaggcat
     6601 cggttgacta cgctcgcaaa tacaagaact tcgttatggg tttcgtgtcg acgcgggccc
     6661 tgacggaagt gcagtcggat gtgtcttcag cctcggagga tgaagatttc gtggtcttca
     6721 cgacgggtgt gaacctctct tccaaaggag ataagcttgg acagcaatac cagactcctg
     6781 catcggctat tggacgcggt gccgacttta tcatcgccgg tcgaggcatc tacgctgctc
     6841 ccgacccggt tgaagctgca cagcggtacc agaaagaagg ctgggaagct tatatggcca
     6901 gagtatgcgg caagtcatga tttcctcttg gagcaaaagt gtagtgccag tacgagtgtt
     6961 gtggaggaag gctgcataca ttgtgcctgt cattaaacga tgagctcgtc cgtattggcc
     7021 cctgtaatgc catgttttcc gcccccaatc gtcaaggttt tccctttgtt agattcctac
     7081 cagtcatcta gcaagtgagg taagctttgc cagaaacgcc aaggctttat ctatgtagtc
     7141 gataagcaaa gtggactgat agcttaatat ggaaggtccc tcaggacaag tcgacctgtg
     7201 cagaagagat aacagcttgg catcacgcat cagtgcctcc tctcagacag aataagtcgc
     7261 cataagttat cgaccgaacc taggtagggt atatttggtg acgacaatac ctcccgacga
     7321 tacctggatt agacaccgta tgccgcgaat taacgccttc cgaccaagtc ccttttggtg
     7381 atcgacactt taggatcatc tcctgccagt cgcccagact gacggtgtta tggtggcgcg
     7441 ctatggcttt cctagccggt caagctccgt gcactagaga gcggagaagg agggggagtt
     7501 gatagggaat gatatctagg tagagaagta ctccaaccag agatatgcgg atcaatgctc
     7561 cggcagttgt tagcataccc caacctcgtt taagtatcct cttcttccat ccatctttat
     7621 cattatttgt ctatgtgcat ccctagatca cgtcaattat ttcgataaat ggatacgtgc
     7681 actcgggaat gcgtgattgg ctcgtctcgt gttctcagtt cctcattccc ccccacgtta
     7741 cattgacaac ggagtattag cctatggtcc tgttcgataa gcttgctcca tcgcctccac
     7801 tacgtcaagc ggagtatcag acatgagctc taccccagat atcgcctgct tatcgctggt
     7861 tgctttgtca tgcaagtaca gatcacatcg gcaattctga taacagatgg tgccattgtc
     7921 ccgtaatcaa ttgcccattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7981 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     8041 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     8101 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     8161 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     8221 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     8281 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     8341 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     8401 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     8461 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     8521 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     8581 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     8641 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8701 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8761 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8821 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8881 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8941 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     9001 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     9061 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     9121 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     9181 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     9241 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     9301 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     9361 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     9421 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     9481 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     9541 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     9601 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9661 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9721 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9781 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9841 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9901 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9961 ggaaacagct atgaccatga ttacgccact gaacgaagca tctgtgcttc attttgtaga
    10021 acaaaaatgc aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac
    10081 agaacagaaa tgcaacgcga aagcgctatt ttaccaacga agaatctgtg cttcattttt
    10141 gtaaaacaaa aatgcaacgc gagagcgcta atttttcaaa caaagaatct gagctgcatt
    10201 tttacagaac agaaatgcaa cgcgagagcg ctattttacc aacaaagaat ctatacttct
    10261 tttttgttct acaaaaatgc atcccgagag cgctattttt ctaacaaagc atcttagatt
    10321 actttttttc tcctttgtgc gctctataat gcagtctctt gataactttt tgcactgtag
    10381 gtccgttaag gttagaagaa ggctactttg gtgtctattt tctcttccat aaaaaaagcc
    10441 tgactccact tcccgcgttt actgattact agcgaagctg cgggtgcatt ttttcaagat
    10501 aaaggcatcc ccgattatat tctataccga tgtggattgc gcatactttg tgaacagaaa
    10561 gtgatagcgt tgatgattct tcattggtca gaaaattatg aacggtttct tctattttgt
    10621 ctctatatac tacgtatagg aaatgtttac attttcgtat tgttttcgat tcactctatg
    10681 aatagttctt actacaattt ttttgtctaa agagtaatac tagagataaa cataaaaaat
    10741 gtagaggtcg agtttagatg caagttcaag gagcgaaagg tggatgggta ggttatatag
    10801 ggatatagca cagagatata tagcaaagag atacttttga gcaatgtttg tggaagcggt
    10861 attcgcaata ttttagtagc tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg
    10921 tcttcagagc gcttttggtt ttcaaaagcg ctctgaagtt cctatacttt ctagagaata
    10981 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
    11041 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
    11101 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
    11161 gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
    11221 tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
    11281 ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcatacta
    11341 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
    11401 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
    11461 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
    11521 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
    11581 gcaccatacc accttttcaa ttcatcattt tttttttatt cttttttttg atttcggttt
    11641 ccttgaaatt tttttgattc ggtaatctcc gaacagaagg aagaacgaag gaaggagcac
    11701 agacttagat tggtatatat acgcatatgt agtgttgaag aaacatgaaa ttgcccagta
    11761 ttcttaaccc aactgcacag aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa
    11821 gctacatata aggaacgtgc tgctactcat cctagtcctg ttgctgccaa gctatttaat
    11881 atcatgcacg aaaagcaaac aaacttgtgt gcttcattgg atgttcgtac caccaaggaa
    11941 ttactggagt tagttgaagc attaggtccc aaaatttgtt tactaaaaac acatgtggat
    12001 atcttgactg atttttccat ggagggcaca gttaagccgc taaaggcatt atccgccaag
    12061 tacaattttt tactcttcga agacagaaaa tttgctgaca ttggtaatac agtcaaattg
    12121 cagtactctg cgggtgtata cagaatagca gaatgggcag acattacgaa tgcacacggt
    12181 gtggtgggcc caggtattgt tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa
    12241 cctagaggcc ttttgatgtt agcagaattg tcatgcaagg gctccctatc tactggagaa
    12301 tatactaagg gtactgttga cattgcgaag agcgacaaag attttgttat cggctttatt
    12361 gctcaaagag acatgggtgg aagagatgaa ggttacgatt ggttgattat gacacccggt
    12421 gtgggtttag atgacaaggg agacgcattg ggtcaacagt atagaaccgt ggatgatgtg
    12481 gtctctacag gatctgacat tattattgtt ggaagaggac tatttgcaaa gggaagggat
    12541 gctaaggtag agggtgaacg ttacagaaaa gcaggctggg aagcatattt gagaagatgc
    12601 ggccagcaaa actaaaaaac tgtattataa gtaaatgcat gtatactaaa ctcacaaatt
    12661 agagcttcaa tttaattata tcagttatta ccctatgcgg tgtgaaatac cgcacagatg
    12721 cgtaaggaga aaataccgca tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg
    12781 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
    12841 tataaatcaa aagaatagac cgagataggg ttgagtgggg taccgagctc gaattcactg
    12901 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
    12961 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
    13021 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
    13081 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
    13141 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    13201 ccttagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccagcttat
    13261 tttttgtata ctgttttgtg atagcacgaa gtttttccac ggtatcttgt taaaaatata
    13321 tatttgtggc gggcttacct acatcaaatt aataagagac taattataaa ctaaacacac
    13381 aagcaagcta ctttagggta aaagtttata aatgcttttg acgtataaac gttgcttgta
    13441 tttattatta caattaaagg tggatagaaa acctagagac tagttagaaa ctaatctcag
    13501 gtttgcgtta aactaaatca gagcccgaga ggttaacaga acctagaagg ggactagata
    13561 tccgggtagg gaaacaaaaa aaaaaaacaa gacagccaca tattagggag actagttaga
    13621 agctagttcc aggactagga aaataaaaga caatgatacc acagtctagt tgacaactag
    13681 atagattcta gattgaggcc aaagtctctg agatccaggt tagttgcaac taatactagt
    13741 tagtatctag tctcctataa ctctgaagct agaataactt actactatta tcctcaccac
    13801 tgttcagctg cgcaaacgga gtgattgcaa ggtgttcaga gactagttat tgactagtca
    13861 gtgactagca ataactaaca aggtattaac ctaccatgtc tgccatcacc ctgcacttcc
    13921 tcgggctcag cagccttttc ctcctcattt tcatgctcat tttccttgtt taagactgtg
    13981 actagtcaaa gactagtcca gaaccacaaa ggagaaatgt cttaccactt tcttcattgc
    14041 ttgtctcttt tgcattatcc atgtctgcaa ctagttagag tctagttagt gactagtccg
    14101 acgaggactt gcttgtctcc ggattgttgg aggaactctc cagggcctca agatccacaa
    14161 cagagccttc tagaagactg gtcaataact agttggtctt tgtctgagtc tgacttacga
    14221 ggttgcatac tcgctccctt tgcctcgtca atcgatgaga aaaagcgcca aaactcgcaa
    14281 tatggctttg aaccacacgg tgctgagact agttagaatc tagtcccaaa ctagcttgga
    14341 tagcttacct ttgccctttg cgttgcgaca ggtcttgcag ggtatggttc ctttctcacc
    14401 agctgattta gctgccttgc taccctcacg gcggatctgc ataaagagtg gctagaggtt
    14461 ataaattagc actgatccta ggtacggggc tgaatgtaac ttgcctttcc tttctcatcg
    14521 cgcggcaaga caggcttgct caaattccta ccagtcacag gggtatgcac ggcgtacgga
    14581 ccacttgaac tagtcacaga ttagttagca actagtctgc attgaatggc tgtacttacg
    14641 ggccctcgcc attgtcctga tcatttccag cttcaccctc gttgctgcaa agtagttagt
    14701 gactagtcaa ggactagttg aaatgggaga agaaactcac gaattctcga cacccttagt
    14761 attgtggtcc ttggacttgg tgctgctata tattagctaa tacactagtt agactcacag
    14821 aaacttacgc agctcgcttg cgcttcttgg taggagtcgg ggttgggaga acagtgcctt
    14881 caaacaagcc ttcataccat gctacttgac tagtcaggga ctagtcacca agtaatctag
    14941 ataggacttg cctttggcct ccatcagttc cttcatagtg ggaggtccat tgtgcaatgt
    15001 aaactccatg ccgtgggagt tcttgtcctt caagtgcttg accaatatgt ttctgttggc
    15061 agagggaacc tgtcaactag ttaataacta gtcagaaact agtatagcag tagactcact
    15121 gtacgcttga ggcatccctt cactcggcag tagacttcat atggatggat atcaggcacg
    15181 ccattgtcgt cctgtggact agtcagtaac taggcttaaa gctagtcggg tcggcttact
    15241 atcttgaaat ccggcagcgt aagctccccg tccttaactg cctcgagata gtgacagtac
    15301 tctggggact ttcggagatc gttatcgcga atgctcggca tactaatcgt tgactagtct
    15361 tggactagtc ccgagcaaaa aggattggag gaggaggagg aaggtgagag tgagacaaag
    15421 agcgaaataa gagcttcaaa ggctatctct aagcagtatg aaggttaagt atctagttct
    15481 tgactagatt taaaagagat ttcgactagt tatgtacctg gagtttggat ataggaatgt
    15541 gttgtggtaa cgaaatgtaa gggggaggaa agaaaaagtc ggtcaagagg taactctaag
    15601 tcggccattc ctttttggga ggcgctaacc ataaacggca tggtcgactt agagttagct
    15661 cagggaattt agggagttat ctgcgaccac tggactctca ggctttccat tggggtacta
    15721 acatagccat caaatgccgg cccgtgttta atctcctcga gatggctggg ctcacatgcg
    15781 ttctactcaa aaactcttat acctaggccc tctcccaatc gtgtatgttt ggaccttcct
    15841 ctctcacact ttggataaca tcaagcatac
//
