LOCUS       sequence-310124-         687 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses FRB-N-frFAST in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Li C, Tebo AG, Thauvin M, Plamont MA, Volovitch M, Morin X, Vriz S, 
            Gautier A
  TITLE     A Far-Red Emitting Fluorescent Chemogenetic Reporter for In Vivo 
            Molecular Imaging.
  JOURNAL   Angew Chem Int Ed Engl. 2020 Jun 22. doi: 10.1002/anie.202006576.
  PUBMED    32568417
REFERENCE   2  (bases 1 to 687)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Angew Chem 
            Int Ed Engl. 2020 Jun 22. doi: 10.1002/anie.202006576."
COMMENT     Sequence Label: pAG499-FRB-N-frFAST
FEATURES             Location/Qualifiers
     source          1..687
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..33
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             40..309
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQ"
ORIGIN
        1 atggaacaaa agcttatttc tgaagaggac ttggaattcg agatgtggca tgaaggcctg
       61 gaagaggcat ctcgtttgta ctttggggaa aggaacgtga aaggcatgtt tgaggtgctg
      121 gagcccttgc atgctatgat ggaacggggc ccccagactc tgaaggaaac atcctttaat
      181 caggcctatg gtcgagattt aatggaggcc caagagtggt gcaggaagta catgaaatca
      241 gggaatgtca aggacctcac ccaagcctgg gacctctatt atcatgtgtt ccgacgaatc
      301 tcaaagcagg tctccggagg aggcggcagc ggcggagggg gatccatgga gcatgttgcc
      361 tttggcagtg aggacatcga gaacactttg gccaaaatgg acgacggaca actggatggg
      421 ttggcctttg gcgcaattca gctcgatggt gacgggaata tcctgcagta caatgctgct
      481 gaaggagaca tcacaggcag agatcccaaa caggtgattg ggaagaactt attcaaggat
      541 gttgcacctg gaacggtttc ctccgggttt tacggcaaat tcaaggaagg cgtagcgtca
      601 gggaatctga acaccatgtt cgaatggatg ataccgacaa gcaggggacc aaccaaggtc
      661 aagatacaca tgaagaaagc cctttcc
//
