LOCUS       sequence-310143-        3527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Template to synthesize riboprobes for in situ hybridization (ISH) 
            for Olfr266.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Zapiec B, Mombaerts P
  TITLE     The Zonal Organization of Odorant Receptor Gene Choice in the Main 
            Olfactory Epithelium of the Mouse.
  JOURNAL   Cell Rep. 2020 Mar 24;30(12):4220-4234.e5. doi: 
            10.1016/j.celrep.2020.02.110.
  PUBMED    32209480
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2020.02"; journalName: "Cell Rep"; date: 
            "2020-03-24- 24"; volume: "30"; issue: "12"; pages: "4220-4234.e5"
COMMENT     Sequence Label: pISH-Olfr266
FEATURES             Location/Qualifiers
     source          1..3527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     151..170
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     404..421
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    538..559
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(574..579,580..597,598..604)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    612..628
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     617..639
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     636..652
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     636..652
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        670..688
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     670..687
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      1294..1303
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1298..1307
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(1337..1356)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1338..1356)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1363..1380)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1363..1379)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1372..1394)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1520..1975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1607..1626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1817..1838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2053..2157
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2158..2226,2227..3018)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2376..2395)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3189..3527,1..250)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
       61 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
      121 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
      181 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
      241 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
      301 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
      361 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga
      421 ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta
      481 atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa
      541 tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat
      601 gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta
      661 cgccaagcta tttaggtgac actatagaat actcaagcta tgcatccaac gcgttgggag
      721 ctctcccata tggtcgacct gcaggcggcc gcgaattcac tagtgatttt ctggaggtct
      781 gctacacctc taacattgtc cccaagatgt tgctgattat tatagctgac cagaagacta
      841 tctcagtggt tggttgcttg gcacaattct acttctttgg atcccttgct gcaacagagt
      901 gcctcttgct tgctgtgatg tcctatgacc gctacctagc tatctgccag cctctccgat
      961 accccatcct catgactggt tctctctgct ttaggttggc tattgggtct tggttctgct
     1021 gcttcttcct cacagccatc accatggtcc tgctgtgtag acaaaacttc tgtggcccta
     1081 atgaaattga tcacttcttt tgtgactttg cccctttgat ccatctctcc tgcatggata
     1141 cttcactgat tgagaccgtt gcctttgcca cctcttctgc agtcactctg gtcccatttt
     1201 tccttatcac aatatcctac tcttgcatcc tcattgccat cctaagaatc ccatctggca
     1261 caggcaggaa aaaggcttaa tcgaattccc gcggccgcca tggcggccgg gagcatgcga
     1321 cgtcgggccc aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca
     1381 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     1441 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     1501 cagcctgaat ggcgaatgga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     1561 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     1621 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     1681 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     1741 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     1801 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     1861 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     1921 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttcctga
     1981 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatcagg tggcactttt
     2041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     2101 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     2161 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     2221 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     2281 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     2341 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     2401 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     2461 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     2521 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2581 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2641 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2701 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2761 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2821 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2881 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2941 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     3001 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     3061 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     3121 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     3181 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     3241 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     3301 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     3361 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     3421 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     3481 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacaca
//
