LOCUS       sequence-310148-       13254 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual fluorescent ratiometric binary 
            vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 13254)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO1112
FEATURES             Location/Qualifiers
     source          1..13254
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             364..384
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    400..524
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(561..566,567..584,585..591)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             645..1304
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(711..730)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1646..1951
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1830..1849
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1992..2116)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2850..2915
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(2922..2924,2925..2927,2928..3635)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(2967..2988)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3228..3247)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3575..3596
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3670..3922
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3971..3987)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3971..3987)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3984..4006)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3995..4011
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4019..4025,4026..4043,4044..4049))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4064..4085
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4119..4371)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4761..5555)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4872..4891)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5482..5501
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5576..5759)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5612..5791)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    5965..5989
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6510..7301
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7547..8135
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8036..8055
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8289..8306
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8321..8461
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8387..8406)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8547..8569
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8805..8999
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9065..10138)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10567..11196)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12497..12521
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     12709..12731
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12723..12740
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12724..12740
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
      361 atgccaaaaa agaagagaaa ggtagaagac cccactagta caagtttgta caaaaaagct
      421 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      481 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg
      541 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
      601 tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      661 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      721 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      781 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      841 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
      901 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      961 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1021 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     1081 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1141 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1201 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
     1261 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     1321 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     1381 atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac
     1441 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     1501 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     1561 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     1621 gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     1681 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     1741 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     1801 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     1861 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     1921 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     1981 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     2041 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     2101 tcttgtacaa agtggtgggc ccaatggtgt ccaagggcga agctgtgatt aaagaattta
     2161 tgcgttttaa ggtccacatg gaaggatcca tgaatggaca tgaattcgaa atcgaggggg
     2221 agggcgaggg ccgaccttat gagggtacac agaccgcgaa gctaaaggta accaagggag
     2281 gcccactacc ctttagttgg gacatactca gcccgcagtt tatgtatggc agtcgtgcct
     2341 ttataaagca cccagcggat ataccagact actacaaaca gtccttccca gaggggttta
     2401 aatgggagcg agtgatgaac tttgaggacg gtggtgctgt gacggtaact caggacacat
     2461 cactcgaaga cgggactctt atttacaaag taaaactgag agggaccaat tttccaccag
     2521 atggccccgt catgcaaaaa aagacgatgg gttgggaagc atctacagaa cgactctatc
     2581 ccgaggacgg cgtcctcaaa ggcgacatta aaatggcctt gaggttgaaa gacggtggac
     2641 gttacttggc ggacttcaaa acaacttata aagcaaaaaa gccagtgcaa atgcctggag
     2701 cgtacaatgt agatagaaaa cttgatataa cgtcacataa tgaggactat accgtggttg
     2761 agcaatacga gaggtcagaa ggacgacaca gcaccggcgg tatggacgag ttgtacaaga
     2821 cgcgtcataa gcagaaaatc gttgcaccag ttaagcagac gttgaacttt gatttgctta
     2881 aactagcagg tgatgttgaa tccaatcctg gacctggtac catggtgagc aagggcgagg
     2941 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     3001 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagc
     3061 tgatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgggct
     3121 acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     3181 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     3241 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     3301 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     3361 acagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     3421 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     3481 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc taccagtccg
     3541 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3601 ccgccgggat cactctcggc atggacgagc tgtacattgc tgcagcggcc taagagctcg
     3661 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     3721 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     3781 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     3841 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     3901 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattggttc cggaaccaat
     3961 tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4021 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4081 cattaggctg aattaggcgc gcctatttct gaattcccga tctagtaaca tagatgacac
     4141 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat
     4201 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat
     4261 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc
     4321 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggatcat
     4381 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtgcat
     4441 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg gcccgatcat
     4501 attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc
     4561 accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca tgagatcccc
     4621 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     4681 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     4741 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     4801 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     4861 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     4921 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     4981 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg
     5041 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     5101 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     5161 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     5221 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     5281 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     5341 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg
     5401 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     5461 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     5521 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga tccggtgcag
     5581 attatttgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa
     5641 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt
     5701 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg
     5761 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc
     5821 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatgaattca
     5881 gaaataaatt cagcctaatt cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt
     5941 attaagttgt ctaagcgtca atttgtttac accacaatat atcctgccac cagccagcca
     6001 acagctcccc gaccggcagc tcggcacaaa atcaccactc gatacaggca gcccatcagt
     6061 ccgggacggc gtcagcggga gagccgttgt aaggcggcag actttgctca tgttaccgat
     6121 gctattcgga agaacggcaa ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg
     6181 gtagcatgtt gattgtaacg atgacagagc gttgctgcct gtgatcaatt cgggcacgaa
     6241 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac
     6301 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca
     6361 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag
     6421 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg
     6481 gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga
     6541 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg
     6601 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc
     6661 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt
     6721 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg
     6781 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag
     6841 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc
     6901 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg
     6961 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa
     7021 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt
     7081 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact
     7141 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt
     7201 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc
     7261 actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt
     7321 tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata
     7381 attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat
     7441 aagccctaca caaattggga gatatatcat gcatgaccaa aatcccttaa cgtgagtttt
     7501 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7561 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7621 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7681 taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7741 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7801 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7861 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7921 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7981 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8041 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8101 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8161 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8221 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8281 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     8341 ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca atctgctctg
     8401 atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc
     8461 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
     8521 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
     8581 atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg agtggcgacg
     8641 gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt tttgagcggc
     8701 cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc gaccgaaggg
     8761 taggcgcttt ttgcagctct tcggctgtgc gctggccaga cagttatgca caggccaggc
     8821 gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg ccttttttct
     8881 cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt gggttcccaa
     8941 tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg ctatccacag
     9001 gaaagagacc ttttcgacct ttttcccctg ctagggcaat ttgccctagc atctgctccg
     9061 tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc atgactagga
     9121 tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca tttgacccga
     9181 tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca ctgcgttcgt
     9241 agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt gccaggcggt
     9301 agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc gtcagcacgt
     9361 ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg atctcgatgt
     9421 actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag gcttcaccct
     9481 cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg gcaacgtgcg
     9541 tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg ccatcggctc
     9601 gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg ggcttgtctc
     9661 ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc atcagtacca
     9721 ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct acgtgcccgt
     9781 ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac agacggaaaa
     9841 cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc atcggaacga
     9901 aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca ccggctgccg
     9961 gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat tcaccggggc
    10021 gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac ttctccacca
    10081 ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga ccgctcacgc
    10141 cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcagacaa cccagccgct
    10201 tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt cggtgcctgg
    10261 ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac tcatttattc
    10321 atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg gtaatggtct
    10381 tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc aactgaaagt
    10441 tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc ttgctgctgc
    10501 gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc tctttacctc
    10561 attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct cgcgggcagc
    10621 gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc ctgggtagct
    10681 cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca gcgcctcggc
    10741 aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg ccgcttgtag
    10801 ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg cggtggccca
    10861 tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct tgatcgcgga
    10921 cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc gccggccgat
    10981 ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta gcggttgatc
    11041 ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga ctaacagaac
    11101 atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg tcttgcctga
    11161 cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg tatttgttta
    11221 tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta ctcaaataca
    11281 catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc cggccaggcc
    11341 gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt tgagacgtgc
    11401 gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc ttcggtaatg
    11461 aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct tggcgttcat
    11521 tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag gcaccgcgcc
    11581 gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac agggtcgagc
    11641 gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg tcgatcagct
    11701 cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac ttcacgcctc
    11761 gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa cgctcgaact
    11821 cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg gtgtcggccc
    11881 acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg gcaatgtcca
    11941 gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca acgtcgccag
    12001 ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg ccggtgatct
    12061 tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg ttggtcaagt
    12121 cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg ctcttgttca
    12181 tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta aaacacgcga
    12241 caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag gacttgtgcg
    12301 acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca ctatagcagc
    12361 ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg catgcacata
    12421 caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta ttctaataaa
    12481 cgctcttttc tcttaggttt acccgccaat atatcctgtc aaacactgat agtttaaact
    12541 gaaggcggga aacgacaatc tgatccaagc tcaagctgct ctagcattcg ccattcaggc
    12601 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    12661 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    12721 gttgtaaaac gacggccagt gccaagcttg catgcctgca ggtccccaga ttagcctttt
    12781 caatttcaga aagaatgcta acccacagat ggttagagag gcttacgcag caggtctcat
    12841 caagacgatc tacccgagca ataatctcca ggaaatcaaa taccttccca agaaggttaa
    12901 agatgcagtc aaaagattca ggactaactg catcaagaac acagagaaag atatatttct
    12961 caagatcaga agtactattc cagtatggac gattcaaggc ttgcttcaca aaccaaggca
    13021 agtaatagag attggagtct ctaaaaaggt agttcccact gaatcaaagg ccatggagtc
    13081 aaagattcaa atagaggacc taacagaact cgccgtaaag actggcgaac agttcataca
    13141 gagtctctta cgactcaatg acaagaagaa aatcttcgtc aacatggtgg agcacgacac
    13201 acttgtctac tccaaaaata tcaaagatac agtctcagaa gaccaaaggg caat
//
