LOCUS       sequence-310156-       13414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector to introduce a constitutive rtta3G cassette and a 
            DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE 
            acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine 
            selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Nora EP, Caccianini L, Fudenberg G, So K, Kameswaran V, Nagle A, 
            Uebersohn A, Hajj B, Saux AL, Coulon A, Mirny LA, Pollard KS, Dahan 
            M, Bruneau BG
  TITLE     Molecular basis of CTCF binding polarity in genome folding.
  JOURNAL   Nat Commun. 2020 Nov 5;11(1):5612. doi: 10.1038/s41467-020-19283-x.
  PUBMED    33154377
REFERENCE   2  (bases 1 to 13414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2020-11-5"; volume: "11(1):5612. doi"; pages: " 
            10.1038/s41467-020-19283-x"
COMMENT     Sequence Label: pEN366 -
            pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G...
FEATURES             Location/Qualifiers
     source          1..13414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1380..1729
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    1388..1406
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1424..1442
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1460..1478
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1496..1514
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1532..1550
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1568..1586
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1604..1622
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1668..1692
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1844..1909
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             4142..4852
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     polyA_signal    4874..5098
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5131..5266
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    5371..5392
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5407..5412,5413..5430,5431..5437)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5445..5461
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5469..5485
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5828..5847
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    5846..5901
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     5947..5966
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(6045..6791)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKGKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     primer_bind     complement(6888..6907)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          6915..7931
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(6969..6991)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     enhancer        8209..8512
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    8576..8609
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(8708..8731)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     9081..9100
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        9160..9207
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9226..9825
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9226..9245)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9722..9742
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    9863..10087
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    10142..10175
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     complement(11193..11209)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11217..11233
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11241..11247,11248..11265,11266..11271))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11286..11307
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11424..11441)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(11595..12183)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11675..11694)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12354..13145,13146..13214))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12977..12996
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13215..13319)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtattta aatactgccc ctaggagatg
      421 gatactagca tttgaaactg aggctcatat gtgttatcac tcaaagtgag gtcagactgc
      481 taggaagtta gggaagtggg atttgagcca ctgaaggtcg gaatctgaaa tctggcgagg
      541 tggtaggaag aggtaggaag gtacccaaca gcttcgaaca ggaggaactc ccagatcctt
      601 acaccaaaaa ccacttaggg ttttttgttt tgtttttgtt ttttaaattt cttttctttt
      661 cttccatggg caaagaaaat gactcaggaa gactcagtgg caaccctaga ctcagggcct
      721 cggaagaggc ccacctctgc atcatcctct ccggagcccc ctgagttcag cactttccgg
      781 gcctgccagt gaggctgacg aatgaggacc actttatcca gttccttccc tcccactgcc
      841 agaggctgca catctgtcca gagacttggc agtggaggta gggtgggggt gggaatcaag
      901 ccatagcttt cttagggaag cactggccaa aggaagggga ctcctagagt tgtaaccttc
      961 ctcacagaag acaagaaaat gagttggggt atcagcctca ggctagacag agagccagaa
     1021 cctcttcaca gattcccaga tcaccggaga agtcactatt gaatccggac acccagtcag
     1081 gcacaagagg tctacattct ctaactcctt tccagtgctt ccccaacggt cacttacttc
     1141 cagactgcgt gttttatttt taggagagat gtgtatattt tttgttgctg ttgttgtttc
     1201 tagatagggt ctcactgtgt agccctggct tttctggaac tcactgtgta gagcaagcca
     1261 gcctcaaact catagatcca cctgcctctg cctccagatc gctagaatta aagttactgc
     1321 cataacacct aacatttaaa tcctgcaggg ggtatcacga ggccctttcg tcttcactcg
     1381 agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga
     1441 gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag tttactccct
     1501 atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac
     1561 agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga
     1621 gaacgtatgt cgaggtaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg
     1681 aaccgtcaga tcgcctggag caattccaca acacttttgt cttatactta cgcgtctgca
     1741 ggatatcaag cttgcggtac cgcggccccg aattcgagct cggtacccgg ggatcctcta
     1801 gtcagctgac gcgtgctagc gcggccgcat cgattccacc atggactata aggaccacga
     1861 cggagactac aaggatcatg atattgatta caaagacgat gacgataaga ccggtgaagg
     1921 tgaggcggtt gaagccattg tggaggagtc tgaaactttc attaaaggaa aagaaagaaa
     1981 gacttaccag agacgccggg aagggggcca ggaagaagat gcttgccacc tgccccagaa
     2041 ccagacagat gggggtgagg tggtccagga tgtcaacagc agtgtacaga tggtaatgat
     2101 ggaacagctg gatcctaccc ttctccagat gaagactgaa gtcatggagg gtacagtggc
     2161 tccggaagca gaggctgcag tggacgatac ccagatcata accttgcagg ttgtaaatat
     2221 ggaggaacag cccattaaca taggagagct tcagcttgtc caagtacctg ttcctgtgac
     2281 ggtacctgtt gctactactt cagtagaaga acttcagggg gcttatgaga atgaagtgtc
     2341 taaagagggc cttgcagaaa gtgaaccgat gatatgtcac accttacctt tgcctgaagg
     2401 atttcaggtg gtgaaagtgg gggccaatgg agaagtggag acactagagc agggcgagct
     2461 tcctcctcag gaagactcta gctggcaaaa agacccagac tatcagccac cagccaaaaa
     2521 aacaaagaaa accaaaaaga gcaaacttcg ttacacagaa gagggcaaag acgtggatgt
     2581 gtctgtgtat gattttgagg aagaacagca ggaaggactg ctgtctgagg ttaatgcaga
     2641 gaaagtagtt ggtaatatga agcctccgaa gccaacaaaa attaaaaaaa aaggtgtaaa
     2701 gaaaacattc cagtgtgagc tttgcagtta cacatgtccc cggcgttcaa atttggatcg
     2761 tcacatgaaa agccacactg atgagagacc acacaaatgc catctgtgtg gcagagcatt
     2821 cagaacagtg accctcctga ggaatcatct gaacacacac acaggtactc gtcctcacaa
     2881 gtgcccagac tgcgatatgg cctttgtgac cagtggagaa ttggtgcggc atcgtcgtta
     2941 taaacacact catgagaaac catttaagtg ttccatgtgt gattatgcca gtgtagaagt
     3001 cagcaaatta aaacgacaca ttcgctctca tactggagag cgcccgttcc agtgcagttt
     3061 gtgcagttat gccagcaggg acacatacaa gctgaaaagg catatgagaa cccattcagg
     3121 ggaaaaacct tatgaatgtt atatttgtca cgctcggttt acccagagtg gtaccatgaa
     3181 gatgcacatt ttacagaagc acacagaaaa tgtggccaaa tttcattgtc cccattgtga
     3241 cactgtcata gcccgaaaaa gtgatttggg tgtccacttg cgaaagcagc attcctatat
     3301 tgaacagggc aaaaaatgtc gctactgtga tgctgtgttt catgagcgat atgctctcat
     3361 ccagcatcaa aaatcacaca aaaatgagaa gcgcttcaag tgtgaccagt gtgattatgc
     3421 ttgtagacag gagcggcaca tgatcatgca caagcgcact cacacggggg agaagcctta
     3481 tgcctgcagc cactgcgaca agaccttccg ccagaaacag ctcctcgaca tgcatttcaa
     3541 gcgctatcat gatcccaact ttgtccctgc tgcctttgtc tgttccaagt gtgggaaaac
     3601 attcacccgc cggaacacaa tggcaagaca tgcagataac tgtgctggtc cagatggcgt
     3661 agagggggaa aatggagggg agacaaagaa gagcaaacga ggaagaaaaa gaaagatgcg
     3721 atctaaaaaa gaagactcct ctgacagtga agaaaatgct gagccggatc tcgatgacaa
     3781 tgaggaggag gaggagcctg ctgtagaaat tgaacctgag ccagagcctc agccccagcc
     3841 tccgcctcca cctcagcctg tggccccggc ccccccacct gccaagaaga gaagggggcg
     3901 acctcctgga agaaccaacc agcccaaaca gaaccagcca acagccatca ttcaggtcga
     3961 agatcagaat acaggtgcaa ttgagaacat tatagttgaa gtcaaaaagg agccagatgc
     4021 cgagcctgcg gagggggaag aagaggaggc tcaggcagcc accacagacg cccccaacgg
     4081 agacctcacg cctgagatga tcctcagcat gatggaccgg cttaaggggg gagcaggagg
     4141 catggtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga
     4201 aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca atccgtacat
     4261 gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga
     4321 cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc cgaaaggcat
     4381 tcctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata
     4441 cgaagatggt ggagtcgtca ccgtcatgca ggacaccagc cttgaggatg gctgtctcgt
     4501 ttaccacgtc caagtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa
     4561 gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagggg
     4621 atacactcat atggcactga aagttgatgg tggtggccat ctgtcttgct ctttcgtaac
     4681 aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtatcc atgccgttga
     4741 tcaccgcctg gaaaggttag aggaaagtga caatgaaatg ttcgtagtac aacgcgaaca
     4801 cgcagttgcc aagttcgccg ggcttggtgg tgggatggac gagctgtaca agtaagttta
     4861 aacatagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4921 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4981 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     5041 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     5101 tctgaggcgg aaagaacctt aattaaccta ggcctccaaa aaagcctcct cactacttct
     5161 ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc
     5221 catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg
     5281 gcgggactat ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga
     5341 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc
     5401 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca
     5461 atttcacaca ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg
     5521 atctccataa gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc
     5581 tggctagtaa aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac
     5641 aaaatataaa aaaaatctaa cctcaagtca aggcttttct atggaataag gaatggacag
     5701 cagggggctg tttcatatac tgatgacctc tttatagcca acctttgttc atggcagcca
     5761 gcatatgggc atatgttgcc aaactctaaa ccaaatactc attctgatgt tttaaatgat
     5821 ttgccctccc atatgtcctt ccgagtgaga gacacaaaaa attccaacac actattgcaa
     5881 tgaaaataaa tttcctttat tagccagaag tcagatgctc aaggggcttc atgatgtccc
     5941 cataattttt ggcagaggga aaaagatctc agtggtattt gtgagccagg gcattggcca
     6001 caccagccac caccttctga taggcagcct gcacctgagg ccgcttaccc ggggagcatg
     6061 tcaaggtcaa aatcgtcaag agcgtcagca ggcagcatat caaggtcaaa gtcgtcaagg
     6121 gcatcggctg ggagcatgtc taagtcaaaa tcgtcaaggg cgtcggtcgg cccgccgctt
     6181 tcgcacttta gctgtttctc caggccacat atgattagtt ccaggccgaa aaggaaggca
     6241 ggttcggctc cctgccggtc gaacagctca attgcttgtt tcagaagtgg gggcatagaa
     6301 tcggtggtag gtgtctctct ttcctctttt gctacttgat gctcctgttc ctccaatacg
     6361 cagcccagtg taaagtggcc cacggcggac agagcgtaca gtgcgttctc cagggagaag
     6421 ccttgctgac acaggaacgc gagctgattt tccagggttt cgtactgttt ctctgttggg
     6481 cgggtgccga gatgcacttt agccccgtcg cgatgtgaga ggagagcaca gcggtatgac
     6541 ttggcgttgt tccgcagaaa gtcttgccat gactcgcctt ccagggggca ggagtgggta
     6601 tgatgcctgt ccagcatctc gattggcagg gcatcgagca gggcccgctt gttcttcacg
     6661 tgccagtaca gggtaggctg ctcaactccc agcttttgag cgagtttcct tgtcgtcagg
     6721 ccttcgatac cgactccatt gagtaattcc agagcagagt ttatgacttt gcccttgtcc
     6781 agtctagaca tggtggcggc ggatcccggg cccgcggtac cgcaagcttg atatcctgca
     6841 gacgcgtgag aattctcgag gaattctttg ccaaaatgat gagacagcac aataaccagc
     6901 acgttgccca ggagctgtag gaaaaagaag aaggcatgaa catggttagc agaggctcta
     6961 gagccgccgg tcacacgcca gaagccgaac cccgccctgc cccgtccccc ccgaaggcag
     7021 ccgtcccccc gcggacagcc ccgaggctgg agagggagaa ggggacggcg gcgcggcgac
     7081 gcacgaaggc cctccccgcc catttccttc ctgccggcgc cgcaccgctt cgccccgcgc
     7141 ccgctagagg gggtgcggcg gcgcctccca gatttcggct ccgcacagat ttgggacaaa
     7201 ggaagtccct gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg
     7261 cctcgacagc cgccggcgct ccgggggccg ccgcgcccct cccccgagcc ctccccggcc
     7321 cgaggcggcc ccgccccgcc cggcaccccc acctgccgcc accccccgcc cggcacggcg
     7381 agccccgcgc cacgccccgt acggagcccc gcacccgaag ccgggccgtg ctcagcaact
     7441 cggggagggg ggtgcagggg gggttgcagc ccgaccgacg cgcccacacc ccctgctcac
     7501 ccccccacgc acacaccccg cacgcagcct ttgttcccct cgcagccccc cccgcaccgc
     7561 ggggcaccgc ccccggccgc gctcccctcg cgcacactgc ggagcgcaca aagccccgcg
     7621 ccgcgcccgc agcgctcaca gccgccgggc agcgcggagc cgcacgcggc gctccccacg
     7681 cacacacaca cgcacgcacc ccccgagccg ctccccccgc acaaagggcc ctcccggagc
     7741 ccctcaaggc tttcacgcag ccacagaaaa gaaacaagcc gtcattaaac caagcgctaa
     7801 ttacagcccg gaggagaagg gccgtcccgc ccgctcacct gtgggagtaa cgcggtcagt
     7861 cagagccggg gcgggcggcg cgaggcggcg gcggagcggg gcacggggcg aaggcagcgc
     7921 gcagcgactc ccgcccgccg cgcgcttcgc tttttatagg gccgccgccg ccgccgcctc
     7981 gccataaaag gaaactttcg gagcgcgccg ctctgattgg ctgccgccgc acctctccgc
     8041 ctcsccccsc cccsccccyc sccccscccc sccccsccyg gcgcgcsccc cccccccccc
     8101 cgcccccatc gctgcacaaa ataattaaaa aataaataaa tacaaaattg ggggtgggga
     8161 ggggggggag atggggagag tgaagcagaa cgtggggctc acctcgacca tggtaatagc
     8221 gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc
     8281 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg
     8341 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     8401 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     8461 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     8521 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcgaagt
     8581 tcctattctc tagaaagtat aggaacttca ggtctgaaga ggagtttacg tccagccaag
     8641 ctagcttggc tgcaggtcgt cgaaattcta ccgggtaggg gaggcgcttt tcccaaggca
     8701 gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa gtggcctctg
     8761 gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct ttggtggccc
     8821 cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc cgcagctcgc
     8881 gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc agatggacag
     8941 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     9001 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggg cgggcgcccg
     9061 aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt
     9121 ctcctcttcc tcatctccgg gcctttcgac ctgcagcctg ttgacaatta atcatcggca
     9181 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatgac cgagtacaag
     9241 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     9301 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     9361 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     9421 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     9481 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     9541 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     9601 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     9661 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     9721 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     9781 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagggga tcaattctct
     9841 agagctcgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc
     9901 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat
     9961 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg
    10021 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc
    10081 tctatggctt ctgaggcgga aagaaccagc tggggctcga ctagagcttg cggaaccctt
    10141 cgaagttcct attctctaga aagtatagga acttcggcgc gccatttaaa ttttgggatg
    10201 ttttctgttt gttttgagat agggtttttc agtgtagtcc tggctgtcct agaactcact
    10261 ctgtagacca ggctggcctg gaactcaagg actgccagcc tctgcctcct gagcatcggg
    10321 attaaaggtg tgtgccacca ctgcccagca gagtgcgttt ttactcttca ctttgtgggt
    10381 cctggctgcc tgagattgtg tgcttgcacc attagatggg agggatgact tgggaaaggg
    10441 ttgagtcttt tgtgagtctt gcggaggccc aggagggggt acgtaaatta gagttctaag
    10501 aggcccagca aaagtgaaca agtgactggc cactgtgagt tcactggggg acctgagagt
    10561 taactagtga aaaggagagg tcctagtgga gaagctaaag aattaaagtt actgccttga
    10621 agttctttgt gaaggccaag ctctggagcg cggcttgaaa agcctttatc ccagagcagc
    10681 atgggaaggg gccccagctg ggtgcgagcc tgcaaaccag agcacaccta tcacagggcc
    10741 ttttcttacc agtgggacgg gatcaaggcg gagcttacct gggaacgtac agggcggaac
    10801 cttaacttgg gggcgtggcc gaatcacctg ggtttggaaa cggttcttgt ctttcctcaa
    10861 gctcaggaga ggttgtttgc aggacactgg cccggagggg cccgaactct ccgagggagg
    10921 gtttcgtgga tcagaacacc gtcccttgac aaaaagatgc agagcaccgt ctgcattctg
    10981 ctagtaaccg tccctcctgg gggcggagcc agccctcctc cgggtgtata aagtagctga
    11041 gcgcctcctt ctgacaaacg cctcaccccc aagagtactg ggggagtacg cagcacgcca
    11101 gcctaggggc ggagctcagc cctggaggag gcgtttcttc ttagttctcc gactcacgcc
    11161 tctccacttt tcatttaaat ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
    11221 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
    11281 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
    11341 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
    11401 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
    11461 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
    11521 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
    11581 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
    11641 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
    11701 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
    11761 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
    11821 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
    11881 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
    11941 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
    12001 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
    12061 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
    12121 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
    12181 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    12241 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    12301 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    12361 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    12421 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    12481 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    12541 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    12601 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    12661 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    12721 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    12781 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    12841 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    12901 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    12961 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    13021 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    13081 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    13141 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    13201 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    13261 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    13321 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
    13381 tataaaaata ggcgtatcac gaggcccttt cgtc
//
