LOCUS       sequence-310160-       13320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual fluorescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13320)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 13320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO1212
FEATURES             Location/Qualifiers
     source          1..13320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             364..384
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    400..524
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(561..566,567..584,585..591)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             645..1304
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(711..730)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1646..1951
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1830..1849
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1992..2116)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2916..2981
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             join(2988..2990,2991..2993,2994..3701)
                     /codon_start=1
                     /product="yellow fluorescent protein (YFP) with fast and 
                     efficient maturation (Nagai et al., 2002)"
                     /label=Venus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(3033..3054)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3294..3313)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3641..3662
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     terminator      3736..3988
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4037..4053)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4037..4053)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4050..4072)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4061..4077
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4085..4091,4092..4109,4110..4115))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4130..4151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4185..4437)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4827..5621)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4938..4957)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     5548..5567
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(5642..5825)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(5678..5857)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6031..6055
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6576..7367
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7613..8201
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8102..8121
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8355..8372
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8387..8527
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8453..8472)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8613..8635
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8871..9065
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9131..10204)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10633..11262)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12563..12587
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     12775..12797
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     12789..12806
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12790..12806
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
      361 atgccaaaaa agaagagaaa ggtagaagac cccactagta caagtttgta caaaaaagct
      421 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      481 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg
      541 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
      601 tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      661 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      721 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      781 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      841 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
      901 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      961 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1021 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     1081 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1141 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1201 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
     1261 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     1321 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     1381 atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac
     1441 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     1501 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     1561 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     1621 gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     1681 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     1741 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     1801 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     1861 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     1921 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     1981 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     2041 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     2101 tcttgtacaa agtggtgggc ccaatggtgt ccaagggcga agctgtgatt aaagaattta
     2161 tgcgttttaa ggtccacatg gaaggatcca tgaatggaca tgaattcgaa atcgaggggg
     2221 agggcgaggg ccgaccttat gagggtacac agaccgcgaa gctaaaggta accaagggag
     2281 gcccactacc ctttagttgg gacatactca gcccgcagtt tatgtatggc agtcgtgcct
     2341 ttataaagca cccagcggat ataccagact actacaaaca gtccttccca gaggggttta
     2401 aatgggagcg agtgatgaac tttgaggacg gtggtgctgt gacggtaact caggacacat
     2461 cactcgaaga cgggactctt atttacaaag taaaactgag agggaccaat tttccaccag
     2521 atggccccgt catgcaaaaa aagacgatgg gttgggaagc atctacagaa cgactctatc
     2581 ccgaggacgg cgtcctcaaa ggcgacatta aaatggcctt gaggttgaaa gacggtggac
     2641 gttacttggc ggacttcaaa acaacttata aagcaaaaaa gccagtgcaa atgcctggag
     2701 cgtacaatgt agatagaaaa cttgatataa cgtcacataa tgaggactat accgtggttg
     2761 agcaatacga gaggtcagaa ggacgacaca gcaccggcgg tatggacgag ttgtacaaga
     2821 cgcgttctaa tgccgccgat gaggttgcga ctggatcagg tcttttagcg atacatccga
     2881 ccgaagcgcg acataaacaa aaaattgtag cgccggtcaa acaaaccctg aatttcgact
     2941 tactcaaact tgcgggtgac gtagagtcaa atccagggcc tggtaccatg gtgagcaagg
     3001 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3061 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3121 tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3181 tgggctacgg cctgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct
     3241 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3301 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3361 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3421 actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
     3481 acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac cactaccagc
     3541 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagctacc
     3601 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3661 tgaccgccgc cgggatcact ctcggcatgg acgagctgta cattgctgca gcggcctaag
     3721 agctcgaatt tccccgatcg ttcaaacatt tggcaataaa gtttcttaag attgaatcct
     3781 gttgccggtc ttgcgatgat tatcatataa tttctgttga attacgttaa gcatgtaata
     3841 attaacatgt aatgcatgac gttatttatg agatgggttt ttatgattag agtcccgcaa
     3901 ttatacattt aatacgcgat agaaaacaaa atatagcgcg caaactagga taaattatcg
     3961 cgcgcggtgt catctatgtt actagatcgg gaattggttc cggaaccaat tggttccgga
     4021 accaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     4081 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     4141 aactcacatt aggctgaatt aggcgcgcct atttctgaat tcccgatcta gtaacataga
     4201 tgacaccgcg cgcgataatt tatcctagtt tgcgcgctat attttgtttt ctatcgcgta
     4261 ttaaatgtat aattgcggga ctctaatcat aaaaacccat ctcataaata acgtcatgca
     4321 ttacatgtta attattacat gcttaacgta attcaacaga aattatatga taatcatcgc
     4381 aagaccggca acaggattca atcttaagaa actttattgc caaatgtttg aacgatcggg
     4441 gatcatccgg gtctgtggcg ggaactccac gaaaatatcc gaacgcagca agatatcgcg
     4501 gtgcatctcg gtcttgcctg ggcagtcgcc gccgacgccg ttgatgtgga cgccgggccc
     4561 gatcatattg tcgctcagga tcgtggcgtt gtgcttgtcg gccgttgctg tcgtaatgat
     4621 atcggcacct tcgaccgcct gttccgcaga gatcccgtgg gcgaagaact ccagcatgag
     4681 atccccgcgc tggaggatca tccagccggc gtcccggaaa acgattccga agcccaacct
     4741 ttcatagaag gcggcggtgg aatcgaaatc tcgtgatggc aggttgggcg tcgcttggtc
     4801 ggtcatttcg aaccccagag tcccgctcag aagaactcgt caagaaggcg atagaaggcg
     4861 atgcgctgcg aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg
     4921 ccgccaagct cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc
     4981 acacccagcc ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc
     5041 ggcaagcagg catcgccatg ggtcacgacg agatcatcgc cgtcgggcat gcgcgccttg
     5101 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
     5161 tcgacaagac cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg
     5221 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
     5281 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
     5341 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
     5401 cccgtcgtgg ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg
     5461 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
     5521 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
     5581 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcgaaacga tccagatccg
     5641 gtgcagatta tttggattga gagtgaatat gagactctaa ttggataccg aggggaattt
     5701 atggaacgtc agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg
     5761 cgacttttga acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga
     5821 aacccgcggc tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg
     5881 cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatg
     5941 aattcagaaa taaattcagc ctaattcggc gttaattcag tacattaaaa acgtccgcaa
     6001 tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc tgccaccagc
     6061 cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata caggcagccc
     6121 atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt tgctcatgtt
     6181 accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg gatgatctcg
     6241 cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga tcaattcggg
     6301 cacgaaccca gtggacataa gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc
     6361 gctcacgcaa ctggtccaga accttgaccg aacgcagcgg tggtaacggc gcagtggcgg
     6421 ttttcatggc ttgttatgac tgtttttttg gggtacagtc tatgcctcgg gcatccaagc
     6481 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc
     6541 agcagggcag tcgccctaaa acaaagttaa acatcatggg ggaagcggtg atcgccgaag
     6601 tatcgactca actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc
     6661 tggccgtaca tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg
     6721 atttgctggt tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg
     6781 accttttgga aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca
     6841 ccattgttgt gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat
     6901 ttggagaatg gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca
     6961 ttgatctggc tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag
     7021 cggcggagga actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa
     7081 ccttaacgct atggaactcg ccgcccgact gggctggcga tgagcgaaat gtagtgctta
     7141 cgttgtcccg catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg
     7201 ccgactgggc aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac
     7261 aggcttatct tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat
     7321 ttgtccacta cgtgaaaggc gagatcacca aggtagtcgg caaataatgt ctagctagaa
     7381 attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag atgcactaag
     7441 cacataattg ctcacagcca aactatcagg tcaagtctgc ttttattatt tttaagcgtg
     7501 cataataagc cctacacaaa ttgggagata tatcatgcat gaccaaaatc ccttaacgtg
     7561 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     7621 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     7681 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     7741 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     7801 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     7861 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     7921 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     7981 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     8041 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     8101 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     8161 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     8221 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     8281 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     8341 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt
     8401 ttctccttac gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct
     8461 gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat
     8521 ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     8581 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     8641 accgtcatca ccgaaacgcg cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg
     8701 gcgacggcgc ggcttgtccg cgccctggta gattgcctgg ccgtaggcca gccatttttg
     8761 agcggccagc ggccgcgata ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc
     8821 gaagggtagg cgctttttgc agctcttcgg ctgtgcgctg gccagacagt tatgcacagg
     8881 ccaggcgggt tttaagagtt ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt
     8941 ttttctcttt tatatcagtc acttacatgt gtgaccggtt cccaatgtac ggctttgggt
     9001 tcccaatgta cgggttccgg ttcccaatgt acggctttgg gttcccaatg tacgtgctat
     9061 ccacaggaaa gagacctttt cgaccttttt cccctgctag ggcaatttgc cctagcatct
     9121 gctccgtaca ttaggaaccg gcggatgctt cgccctcgat caggttgcgg tagcgcatga
     9181 ctaggatcgg gccagcctgc cccgcctcct ccttcaaatc gtactccggc aggtcatttg
     9241 acccgatcag cttgcgcacg gtgaaacaga acttcttgaa ctctccggcg ctgccactgc
     9301 gttcgtagat cgtcttgaac aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca
     9361 ggcggtagag aaaacggccg atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca
     9421 gcacgtccgg gttcttgcct tctgtgatct cgcggtacat ccaatcagct agctcgatct
     9481 cgatgtactc cggccgcccg gtttcgctct ttacgatctt gtagcggcta atcaaggctt
     9541 caccctcgga taccgtcacc aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa
     9601 cgtgcgtggt gtttaaccga atgcaggttt ctaccaggtc gtctttctgc tttccgccat
     9661 cggctcgccg gcagaacttg agtacgtccg caacgtgtgg acggaacacg cggccgggct
     9721 tgtctccctt cccttcccgg tatcggttca tggattcggt tagatgggaa accgccatca
     9781 gtaccaggtc gtaatcccac acactggcca tgccggccgg ccctgcggaa acctctacgt
     9841 gcccgtctgg aagctcgtag cggatcacct cgccagctcg tcggtcacgc ttcgacagac
     9901 ggaaaacggc cacgtccatg atgctgcgac tatcgcgggt gcccacgtca tagagcatcg
     9961 gaacgaaaaa atctggttgc tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg
    10021 ctgccggcgg ttgccgggat tctttgcgga ttcgatcagc ggccgcttgc cacgattcac
    10081 cggggcgtgc ttctgcctcg atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct
    10141 ccaccaggtc atcacccagc gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc
    10201 tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc agacaaccca
    10261 gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca cggcgtcggt
    10321 gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc atctactcat
    10381 ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc agctcggtaa
    10441 tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc gccggcaact
    10501 gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc
    10561 tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc attttctctt
    10621 tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct ggacctcgcg
    10681 ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg
    10741 gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc cggccagcgc
    10801 ctcggcaacc tcaccgccga tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc
    10861 ttgtagcctt ccatccgtga cctcaatgcg ctgcttaacc agctccacca ggtcggcggt
    10921 ggcccatatg tcgtaagggc ttggctgcac cggaatcagc acgaagtcgg ctgccttgat
    10981 cgcggacaca gccaagtccg ccgcctgggg cgctccgtcg atcactacga agtcgcgccg
    11041 gccgatggcc ttcacgtcgc ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg
    11101 ttgatcttcc cgcacggccg cccaatcgcg ggcactgccc tggggatcgg aatcgactaa
    11161 cagaacatcg gccccggcga gttgcagggc gcgggctaga tgggttgcga tggtcgtctt
    11221 gcctgacccg cctttctggt taagtacagc gataaccttc atgcgttccc cttgcgtatt
    11281 tgtttattta ctcatcgcat catatacgca gcgaccgcat gacgcaagct gttttactca
    11341 aatacacatc acctttttag acggcggcgc tcggtttctt cagcggccaa gctggccggc
    11401 caggccgcca gcttggcatc agacaaaccg gccaggattt catgcagccg cacggttgag
    11461 acgtgcgcgg gcggctcgaa cacgtacccg gccgcgatca tctccgcctc gatctcttcg
    11521 gtaatgaaaa acggttcgtc ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc
    11581 gttcattctc ggcggccgcc agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac
    11641 cgcgccgcct ggcctcggtg ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg
    11701 tcgagcgatg cacgccaagc agtgcagccg cctctttcac ggtgcggcct tcctggtcga
    11761 tcagctcgcg ggcgtgcgcg atctgtgccg gggtgagggt agggcggggg ccaaacttca
    11821 cgcctcgggc cttggcggcc tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct
    11881 cgaactcggc aatgccggcg aacacggtca acaccatgcg gccggccggc gtggtggtgt
    11941 cggcccacgg ctctgccagg ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa
    12001 tgtccagtag gtcgcgggtg ctgcgggcca ggcggtctag cctggtcact gtcacaacgt
    12061 cgccagggcg taggtggtca agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg
    12121 tgatcttctc ggaaaacagc ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg
    12181 tcaagtcctg gtcgtcggtg ctgacgcggg catagcccag caggccagcg gcggcgctct
    12241 tgttcatggc gtaatgtctc cggttctagt cgcaagtatt ctactttatg cgactaaaac
    12301 acgcgacaag aaaacgccag gaaaagggca gggcggcagc ctgtcgcgta acttaggact
    12361 tgtgcgacat gtcgttttca gaagacggct gcactgaacg tcagaagccg actgcactat
    12421 agcagcggag gggttggatc aaagtacttt gatcccgagg ggaaccctgt ggttggcatg
    12481 cacatacaaa tggacgaacg gataaacctt ttcacgccct tttaaatatc cgattattct
    12541 aataaacgct cttttctctt aggtttaccc gccaatatat cctgtcaaac actgatagtt
    12601 taaactgaag gcgggaaacg acaatctgat ccaagctcaa gctgctctag cattcgccat
    12661 tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc
    12721 tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt
    12781 cacgacgttg taaaacgacg gccagtgcca agcttgcatg cctgcaggtc cccagattag
    12841 ccttttcaat ttcagaaaga atgctaaccc acagatggtt agagaggctt acgcagcagg
    12901 tctcatcaag acgatctacc cgagcaataa tctccaggaa atcaaatacc ttcccaagaa
    12961 ggttaaagat gcagtcaaaa gattcaggac taactgcatc aagaacacag agaaagatat
    13021 atttctcaag atcagaagta ctattccagt atggacgatt caaggcttgc ttcacaaacc
    13081 aaggcaagta atagagattg gagtctctaa aaaggtagtt cccactgaat caaaggccat
    13141 ggagtcaaag attcaaatag aggacctaac agaactcgcc gtaaagactg gcgaacagtt
    13201 catacagagt ctcttacgac tcaatgacaa gaagaaaatc ttcgtcaaca tggtggagca
    13261 cgacacactt gtctactcca aaaatatcaa agatacagtc tcagaagacc aaagggcaat
//
