LOCUS       sequence-310162-       14904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible ratiometric binary vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14904)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 14904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO2151
FEATURES             Location/Qualifiers
     source          1..14904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1614..1634
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1650..1774
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1811..1816,1817..1834,1835..1841)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1895..2554
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1961..1980)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2896..3201
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3080..3099
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3242..3366)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             5054..5119
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     terminator      5841..6093
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(6126..6142)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6126..6142)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6139..6161)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6150..6166
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6174..6180,6181..6198,6199..6204))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6219..6240
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(6274..6526)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6916..7710)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7027..7046)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     7637..7656
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(7731..7914)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(7767..7946)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    8120..8144
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             8665..9456
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      9702..10290
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10191..10210
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10444..10461
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10476..10616
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10542..10561)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10702..10724
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10960..11154
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11220..12293)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12722..13351)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    14652..14676
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14864..14886
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14878..14895
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14879..14895
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgacgtcgta tgatcgcgaa gcacccaccc taagccacat tttaatcctt ctgttggcca
       61 tgccccatca aagttgcact taacccaaga ttgtggtgga gcttcccatg tttctcgtct
      121 gtcccgacgg tgttgtggtt ggtgctttcc ttacattctg agcctctttc cttctaatcc
      181 actcatctgc atcttcttgt gtccttacta atacctcatt ggttccaaat tccctccctt
      241 taagcaccag ctcgtttctg ttcttccaca gcctcccaag tatccaaggg actaaagcct
      301 ccacattctt cagatcagga tattcttgtt taagatgttg aactctatgg aggtttgtat
      361 gaactgatga tctaggaccg gataagttcc cttcttcata gcgaacttat tcaaagaatg
      421 ttttgtgtat cattcttgtt acattgttat taatgaaaaa atattattgg tcattggact
      481 gaacacgagt gttaaatatg gaccaggccc caaataagat ccattgatat atgaattaaa
      541 taacaagaat aaatcgagtc accaaaccac ttgccttttt taacgagact tgttcaccaa
      601 cttgatacaa aagtcattat cctatgcaaa tcaataatca tacaaaaata tccaataaca
      661 ctaaaaaatt aaaagaaatg gataatttca caatatgtta tacgataaag aagttacttt
      721 tccaagaaat tcactgattt tataagccca cttgcattag ataaatggca aaaaaaaaca
      781 aaaaggaaaa gaaataaagc acgaagaatt ccagaaaata cgaaatacgc ttcaatgcag
      841 tgggacccac ggttcaatta ttgccaattt tcagctccac cgtatattta aaaaataaaa
      901 cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa tctcaacggc tggatcttat
      961 gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg
     1021 cagccggcac acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta
     1081 aaaataaggc aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca
     1141 ttttattatt agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct
     1201 tttcttcttc ttcttctata aaacaatacc caaagagttc ttcttcttca caattcagat
     1261 ttcaatttct caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa
     1321 tttctgtgtt ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg
     1381 tatatgttct ttggtttaga ttctgttaat cttagatcga agacgatttt ctgggtttga
     1441 tcgttagata tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa
     1501 ataatttgag ttttgtcgaa taattactct tcgatttgtg atttctatcc agatctggtg
     1561 ttagtttcta gtttgtgcga tcgaatttgt cctcgagagg ggactctaga atgccaaaaa
     1621 agaagagaaa ggtagaagac cccactagta caagtttgta caaaaaagct gaacgagaaa
     1681 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1741 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1801 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1861 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1921 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1981 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2041 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2101 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2161 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2221 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2281 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2341 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2401 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2461 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2521 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2581 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2641 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt
     2701 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2761 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2821 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2881 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2941 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3001 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3061 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     3121 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3181 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     3241 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     3301 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     3361 agtggtgggc ccaatggaag atgctaagaa tattaagaag ggaccagctc ctttctaccc
     3421 actcgaagat ggaacagcag gtgaacagtt acacaaggct atgaaaagat atgctcttgt
     3481 tcctggtact atcgcattca cagatgctca tatcgaggtg gatataactt atgctgagta
     3541 cttcgaaatg tctgttaggc tcgctgaagc tatgaagaga tacggattaa atacaaacca
     3601 caggattgtt gtgtgtagtg aaaattcttt gcaatttttc atgcctgttc ttggagcttt
     3661 gttcatcggt gtggctgttg caccagctaa tgatatatat aacgagagag aacttcttaa
     3721 ttctatggga atttctcaac ctactgttgt gttcgtgtct aagaaaggac ttcaaaagat
     3781 tttgaacgtt cagaagaaac ttccaattat ccagaagatt attattatgg attcaaagac
     3841 agattaccaa ggtttccagt caatgtacac ctttgtgacc agtcatttgc ctcctggatt
     3901 caatgagtac gatttcgttc cagaaagttt tgatagagat aagactattg ctctcattat
     3961 gaactcttca ggatctacag gtcttcctaa gggtgtggca ttgccacata gaaccgcttg
     4021 tgttaggttc tctcacgcaa gagatcctat tttcggaaac cagataattc cagatacagc
     4081 tatcttgtca gttgtgcctt tccatcacgg atttggaatg ttcactacac tcggatacct
     4141 tatttgtggt tttagggttg tgctcatgta cagattcgaa gaggaactct ttttaagatc
     4201 tttacaagat tataagatac agtcagcact cttagttcca acccttttct ctttctttgc
     4261 taagtcaact ttgatcgata agtacgatct ttctaacttg catgagattg caagtggagg
     4321 tgctcctctt tctaaagaag tgggtgaagc agttgctaag aggtttcact tgccaggaat
     4381 cagacaagga tatggtctta cagagaccac ttctgctatc ttgataaccc ctgaaggaga
     4441 tgataaacca ggagcagttg gaaaggttgt tcctttcttt gaagctaaag ttgtggatct
     4501 cgatacagga aagaccttag gtgtgaatca gaggggagag ctttgcgtta gaggtcctat
     4561 gataatgagt ggatatgtta ataacccaga agcaactaac gctcttatcg ataaagatgg
     4621 atggttgcat tcaggagata ttgcttactg ggatgaggat gaacactttt tcatagtgga
     4681 taggctcaag tcattaatta agtataaggg ataccaagtt gcacctgctg aactcgaaag
     4741 tattcttttg cagcatccaa acatcttcga tgcaggagtt gctggtcttc ctgatgatga
     4801 tgctggagaa ctcccagctg cagttgtggt tttagaacac ggaaagacta tgacagagaa
     4861 agaaattgtg gattacgttg catctcaagt tacaaccgct aagaaactta gaggtggtgt
     4921 ggtttttgtg gatgaggttc caaagggact cactggtaaa ttagatgcta gaaagatcag
     4981 ggaaattttg atcaaagcaa agaaaggagg aaagattgct gttacgcgtc ataagcagaa
     5041 aatcgttgca ccagttaagc agacgttgaa ctttgatttg cttaaactag caggtgatgt
     5101 tgaatccaat cctggacctg gtaccatggt gtccaagggc gaagctgtga ttaaagaatt
     5161 tatgcgtttt aaggtccaca tggaaggatc catgaatgga catgaattcg aaatcgaggg
     5221 ggagggcgag ggccgacctt atgagggtac acagaccgcg aagctaaagg taaccaaggg
     5281 aggcccacta ccctttagtt gggacatact cagcccgcag tttatgtatg gcagtcgtgc
     5341 ctttataaag cacccagcgg atataccaga ctactacaaa cagtccttcc cagaggggtt
     5401 taaatgggag cgagtgatga actttgagga cggtggtgct gtgacggtaa ctcaggacac
     5461 atcactcgaa gacgggactc ttatttacaa agtaaaactg agagggacca attttccacc
     5521 agatggcccc gtcatgcaaa aaaagacgat gggttgggaa gcatctacag aacgactcta
     5581 tcccgaggac ggcgtcctca aaggcgacat taaaatggcc ttgaggttga aagacggtgg
     5641 acgttacttg gcggacttca aaacaactta taaagcaaaa aagccagtgc aaatgcctgg
     5701 agcgtacaat gtagatagaa aacttgatat aacgtcacat aatgaggact ataccgtggt
     5761 tgagcaatac gagaggtcag aaggacgaca cagcaccggc ggtatggacg agttgtacaa
     5821 gtaagagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc ttaagattga
     5881 atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac gttaagcatg
     5941 taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg attagagtcc
     6001 cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac taggataaat
     6061 tatcgcgcgc ggtgtcatct atgttactag atcgggaatt ggttccggaa ccaattcgta
     6121 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     6181 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     6241 ggctgaatta ggcgcgccta tttctgaatt cccgatctag taacatagat gacaccgcgc
     6301 gcgataattt atcctagttt gcgcgctata ttttgttttc tatcgcgtat taaatgtata
     6361 attgcgggac tctaatcata aaaacccatc tcataaataa cgtcatgcat tacatgttaa
     6421 ttattacatg cttaacgtaa ttcaacagaa attatatgat aatcatcgca agaccggcaa
     6481 caggattcaa tcttaagaaa ctttattgcc aaatgtttga acgatcgggg atcatccggg
     6541 tctgtggcgg gaactccacg aaaatatccg aacgcagcaa gatatcgcgg tgcatctcgg
     6601 tcttgcctgg gcagtcgccg ccgacgccgt tgatgtggac gccgggcccg atcatattgt
     6661 cgctcaggat cgtggcgttg tgcttgtcgg ccgttgctgt cgtaatgata tcggcacctt
     6721 cgaccgcctg ttccgcagag atcccgtggg cgaagaactc cagcatgaga tccccgcgct
     6781 ggaggatcat ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg
     6841 cggcggtgga atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga
     6901 accccagagt cccgctcaga agaactcgtc aagaaggcga tagaaggcga tgcgctgcga
     6961 atcgggagcg gcgataccgt aaagcacgag gaagcggtca gcccattcgc cgccaagctc
     7021 ttcagcaata tcacgggtag ccaacgctat gtcctgatag cggtccgcca cacccagccg
     7081 gccacagtcg atgaatccag aaaagcggcc attttccacc atgatattcg gcaagcaggc
     7141 atcgccatgg gtcacgacga gatcatcgcc gtcgggcatg cgcgccttga gcctggcgaa
     7201 cagttcggct ggcgcgagcc cctgatgctc ttcgtccaga tcatcctgat cgacaagacc
     7261 ggcttccatc cgagtacgtg ctcgctcgat gcgatgtttc gcttggtggt cgaatgggca
     7321 ggtagccgga tcaagcgtat gcagccgccg cattgcatca gccatgatgg atactttctc
     7381 ggcaggagca aggtgagatg acaggagatc ctgccccggc acttcgccca atagcagcca
     7441 gtcccttccc gcttcagtga caacgtcgag cacagctgcg caaggaacgc ccgtcgtggc
     7501 cagccacgat agccgcgctg cctcgtcctg cagttcattc agggcaccgg acaggtcggt
     7561 cttgacaaaa agaaccgggc gcccctgcgc tgacagccgg aacacggcgg catcagagca
     7621 gccgattgtc tgttgtgccc agtcatagcc gaatagcctc tccacccaag cggccggaga
     7681 acctgcgtgc aatccatctt gttcaatcat gcgaaacgat ccagatccgg tgcagattat
     7741 ttggattgag agtgaatatg agactctaat tggataccga ggggaattta tggaacgtca
     7801 gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc gacttttgaa
     7861 cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa acccgcggct
     7921 gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg
     7981 tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatga attcagaaat
     8041 aaattcagcc taattcggcg ttaattcagt acattaaaaa cgtccgcaat gtgttattaa
     8101 gttgtctaag cgtcaatttg tttacaccac aatatatcct gccaccagcc agccaacagc
     8161 tccccgaccg gcagctcggc acaaaatcac cactcgatac aggcagccca tcagtccggg
     8221 acggcgtcag cgggagagcc gttgtaaggc ggcagacttt gctcatgtta ccgatgctat
     8281 tcggaagaac ggcaactaag ctgccgggtt tgaaacacgg atgatctcgc ggagggtagc
     8341 atgttgattg taacgatgac agagcgttgc tgcctgtgat caattcgggc acgaacccag
     8401 tggacataag cctgttcggt tcgtaagctg taatgcaagt agcgtatgcg ctcacgcaac
     8461 tggtccagaa ccttgaccga acgcagcggt ggtaacggcg cagtggcggt tttcatggct
     8521 tgttatgact gtttttttgg ggtacagtct atgcctcggg catccaagca gcaagcgcgt
     8581 tacgccgtgg gtcgatgttt gatgttatgg agcagcaacg atgttacgca gcagggcagt
     8641 cgccctaaaa caaagttaaa catcatgggg gaagcggtga tcgccgaagt atcgactcaa
     8701 ctatcagagg tagttggcgt catcgagcgc catctcgaac cgacgttgct ggccgtacat
     8761 ttgtacggct ccgcagtgga tggcggcctg aagccacaca gtgatattga tttgctggtt
     8821 acggtgaccg taaggcttga tgaaacaacg cggcgagctt tgatcaacga ccttttggaa
     8881 acttcggctt cccctggaga gagcgagatt ctccgcgctg tagaagtcac cattgttgtg
     8941 cacgacgaca tcattccgtg gcgttatcca gctaagcgcg aactgcaatt tggagaatgg
     9001 cagcgcaatg acattcttgc aggtatcttc gagccagcca cgatcgacat tgatctggct
     9061 atcttgctga caaaagcaag agaacatagc gttgccttgg taggtccagc ggcggaggaa
     9121 ctctttgatc cggttcctga acaggatcta tttgaggcgc taaatgaaac cttaacgcta
     9181 tggaactcgc cgcccgactg ggctggcgat gagcgaaatg tagtgcttac gttgtcccgc
     9241 atttggtaca gcgcagtaac cggcaaaatc gcgccgaagg atgtcgctgc cgactgggca
     9301 atggagcgcc tgccggccca gtatcagccc gtcatacttg aagctagaca ggcttatctt
     9361 ggacaagaag aagatcgctt ggcctcgcgc gcagatcagt tggaagaatt tgtccactac
     9421 gtgaaaggcg agatcaccaa ggtagtcggc aaataatgtc tagctagaaa ttcgttcaag
     9481 ccgacgccgc ttcgcggcgc ggcttaactc aagcgttaga tgcactaagc acataattgc
     9541 tcacagccaa actatcaggt caagtctgct tttattattt ttaagcgtgc ataataagcc
     9601 ctacacaaat tgggagatat atcatgcatg accaaaatcc cttaacgtga gttttcgttc
     9661 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
     9721 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
     9781 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
     9841 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
     9901 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
     9961 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
    10021 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
    10081 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
    10141 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
    10201 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
    10261 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
    10321 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
    10381 gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag
    10441 cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg
    10501 catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc
    10561 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc
    10621 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
    10681 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
    10741 cgaaacgcgc gaggcagggt gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg
    10801 gcttgtccgc gccctggtag attgcctggc cgtaggccag ccatttttga gcggccagcg
    10861 gccgcgatag gccgacgcga agcggcgggg cgtagggagc gcagcgaccg aagggtaggc
    10921 gctttttgca gctcttcggc tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt
    10981 ttaagagttt taataagttt taaagagttt taggcggaaa aatcgccttt tttctctttt
    11041 atatcagtca cttacatgtg tgaccggttc ccaatgtacg gctttgggtt cccaatgtac
    11101 gggttccggt tcccaatgta cggctttggg ttcccaatgt acgtgctatc cacaggaaag
    11161 agaccttttc gacctttttc ccctgctagg gcaatttgcc ctagcatctg ctccgtacat
    11221 taggaaccgg cggatgcttc gccctcgatc aggttgcggt agcgcatgac taggatcggg
    11281 ccagcctgcc ccgcctcctc cttcaaatcg tactccggca ggtcatttga cccgatcagc
    11341 ttgcgcacgg tgaaacagaa cttcttgaac tctccggcgc tgccactgcg ttcgtagatc
    11401 gtcttgaaca accatctggc ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga
    11461 aaacggccga tgccgggatc gatcaaaaag taatcggggt gaaccgtcag cacgtccggg
    11521 ttcttgcctt ctgtgatctc gcggtacatc caatcagcta gctcgatctc gatgtactcc
    11581 ggccgcccgg tttcgctctt tacgatcttg tagcggctaa tcaaggcttc accctcggat
    11641 accgtcacca ggcggccgtt cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg
    11701 tttaaccgaa tgcaggtttc taccaggtcg tctttctgct ttccgccatc ggctcgccgg
    11761 cagaacttga gtacgtccgc aacgtgtgga cggaacacgc ggccgggctt gtctcccttc
    11821 ccttcccggt atcggttcat ggattcggtt agatgggaaa ccgccatcag taccaggtcg
    11881 taatcccaca cactggccat gccggccggc cctgcggaaa cctctacgtg cccgtctgga
    11941 agctcgtagc ggatcacctc gccagctcgt cggtcacgct tcgacagacg gaaaacggcc
    12001 acgtccatga tgctgcgact atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa
    12061 tctggttgct cgtcgccctt gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt
    12121 tgccgggatt ctttgcggat tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct
    12181 tctgcctcga tgcgttgccg ctgggcggcc tgcgcggcct tcaacttctc caccaggtca
    12241 tcacccagcg ccgcgccgat ttgtaccggg ccggatggtt tgcgaccgct cacgccgatt
    12301 cctcgggctt gggggttcca gtgccattgc agggccggca gacaacccag ccgcttacgc
    12361 ctggccaacc gcccgttcct ccacacatgg ggcattccac ggcgtcggtg cctggttgtt
    12421 cttgattttc catgccgcct cctttagccg ctaaaattca tctactcatt tattcatttg
    12481 ctcatttact ctggtagctg cgcgatgtat tcagatagca gctcggtaat ggtcttgcct
    12541 tggcgtaccg cgtacatctt cagcttggtg tgatcctccg ccggcaactg aaagttgacc
    12601 cgcttcatgg ctggcgtgtc tgccaggctg gccaacgttg cagccttgct gctgcgtgcg
    12661 ctcggacggc cggcacttag cgtgtttgtg cttttgctca ttttctcttt acctcattaa
    12721 ctcaaatgag ttttgattta atttcagcgg ccagcgcctg gacctcgcgg gcagcgtcgc
    12781 cctcgggttc tgattcaaga acggttgtgc cggcggcggc agtgcctggg tagctcacgc
    12841 gctgcgtgat acgggactca agaatgggca gctcgtaccc ggccagcgcc tcggcaacct
    12901 caccgccgat gcgcgtgcct ttgatcgccc gcgacacgac aaaggccgct tgtagccttc
    12961 catccgtgac ctcaatgcgc tgcttaacca gctccaccag gtcggcggtg gcccatatgt
    13021 cgtaagggct tggctgcacc ggaatcagca cgaagtcggc tgccttgatc gcggacacag
    13081 ccaagtccgc cgcctggggc gctccgtcga tcactacgaa gtcgcgccgg ccgatggcct
    13141 tcacgtcgcg gtcaatcgtc gggcggtcga tgccgacaac ggttagcggt tgatcttccc
    13201 gcacggccgc ccaatcgcgg gcactgccct ggggatcgga atcgactaac agaacatcgg
    13261 ccccggcgag ttgcagggcg cgggctagat gggttgcgat ggtcgtcttg cctgacccgc
    13321 ctttctggtt aagtacagcg ataaccttca tgcgttcccc ttgcgtattt gtttatttac
    13381 tcatcgcatc atatacgcag cgaccgcatg acgcaagctg ttttactcaa atacacatca
    13441 cctttttaga cggcggcgct cggtttcttc agcggccaag ctggccggcc aggccgccag
    13501 cttggcatca gacaaaccgg ccaggatttc atgcagccgc acggttgaga cgtgcgcggg
    13561 cggctcgaac acgtacccgg ccgcgatcat ctccgcctcg atctcttcgg taatgaaaaa
    13621 cggttcgtcc tggccgtcct ggtgcggttt catgcttgtt cctcttggcg ttcattctcg
    13681 gcggccgcca gggcgtcggc ctcggtcaat gcgtcctcac ggaaggcacc gcgccgcctg
    13741 gcctcggtgg gcgtcacttc ctcgctgcgc tcaagtgcgc ggtacagggt cgagcgatgc
    13801 acgccaagca gtgcagccgc ctctttcacg gtgcggcctt cctggtcgat cagctcgcgg
    13861 gcgtgcgcga tctgtgccgg ggtgagggta gggcgggggc caaacttcac gcctcgggcc
    13921 ttggcggcct cgcgcccgct ccgggtgcgg tcgatgatta gggaacgctc gaactcggca
    13981 atgccggcga acacggtcaa caccatgcgg ccggccggcg tggtggtgtc ggcccacggc
    14041 tctgccaggc tacgcaggcc cgcgccggcc tcctggatgc gctcggcaat gtccagtagg
    14101 tcgcgggtgc tgcgggccag gcggtctagc ctggtcactg tcacaacgtc gccagggcgt
    14161 aggtggtcaa gcatcctggc cagctccggg cggtcgcgcc tggtgccggt gatcttctcg
    14221 gaaaacagct tggtgcagcc ggccgcgtgc agttcggccc gttggttggt caagtcctgg
    14281 tcgtcggtgc tgacgcgggc atagcccagc aggccagcgg cggcgctctt gttcatggcg
    14341 taatgtctcc ggttctagtc gcaagtattc tactttatgc gactaaaaca cgcgacaaga
    14401 aaacgccagg aaaagggcag ggcggcagcc tgtcgcgtaa cttaggactt gtgcgacatg
    14461 tcgttttcag aagacggctg cactgaacgt cagaagccga ctgcactata gcagcggagg
    14521 ggttggatca aagtactttg atcccgaggg gaaccctgtg gttggcatgc acatacaaat
    14581 ggacgaacgg ataaaccttt tcacgccctt ttaaatatcc gattattcta ataaacgctc
    14641 ttttctctta ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg
    14701 cgggaaacga caatctgatc caagctcaag ctgctctagc attcgccatt caggctgcgc
    14761 aactgttggg aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg
    14821 ggatgtgctg caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt
    14881 aaaacgacgg ccagtgccaa gctt
//
