LOCUS       sequence-310164-       11979 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in 
            E. coli Nissle 1917.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11979)
  AUTHORS   Lakshmanan A, Jin Z, Nety SP, Sawyer DP, Lee-Gosselin A, Malounda D,
            Swift MB, Maresca D, Shapiro MG
  TITLE     Acoustic biosensors for ultrasound imaging of enzyme activity.
  JOURNAL   Nat Chem Biol. 2020 Sep;16(9):988-996. doi: 
            10.1038/s41589-020-0591-0. Epub 2020 Jul 13.
  PUBMED    32661379
REFERENCE   2  (bases 1 to 11979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-020-0591-0"; journalName: "Nat Chem Biol"; date: 
            "2020-09"; volume: "16"; issue: "9"; pages: "988-996"
COMMENT     Sequence Label: pET28a-T5-ASG_ClpXP
FEATURES             Location/Qualifiers
     source          1..11979
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      12..467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(99..118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     309..330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(559..1374)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(559..1374)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1282..1301
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1366..1385
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      1496..2084
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1985..2004
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2238..2255
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    2270..2409
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(2335..2354)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2495..2517
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(2511..2702)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(3511..4593)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     4555..4574
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(4594..4671)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     4877..4896
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(4978..4992,4993..4998,4999..5015,5016..5022)
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    4998..5014
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             8339..8356
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="GSSKSK"
     primer_bind     complement(11893..11911)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      11907..11954
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
       61 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
      121 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
      181 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
      241 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
      301 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
      361 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
      421 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt
      481 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
      541 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
      601 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
      661 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
      721 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
      781 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
      841 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
      901 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca
      961 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
     1021 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
     1081 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
     1141 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
     1201 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
     1261 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
     1321 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcataacacc
     1381 ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa atcccttaac
     1441 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1501 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1561 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1621 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     1681 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1741 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1801 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1861 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1921 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1981 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2041 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2101 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2161 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2221 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     2281 attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc tcagtacaat
     2341 ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc
     2401 atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     2461 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     2521 tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga
     2581 agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc
     2641 gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc
     2701 actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga
     2761 gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt
     2821 gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa
     2881 tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg
     2941 atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa
     3001 acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag
     3061 tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc
     3121 cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ggccgccatg
     3181 ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct
     3241 tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc
     3301 cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt
     3361 tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg
     3421 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     3481 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     3541 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     3601 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     3661 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     3721 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     3781 cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     3841 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     3901 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     3961 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     4021 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     4081 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     4141 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     4201 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     4261 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     4321 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     4381 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     4441 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     4501 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     4561 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     4621 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     4681 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     4741 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     4801 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     4861 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     4921 cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgcatca
     4981 taaaaaattt atttgctttg tgagcggata acaattataa tatttcacac aggaaaccca
     5041 gtcgagctca tgactcatat ttatagttat tctcctcatt tcccaagttt ccgacaacaa
     5101 ataacaagga attaaaaaaa tggcagttga aaaaaccaat tcttcctcca gcttggcaga
     5161 agttattgat agaatcctcg acaaaggtat cgtaattgac gcttgggttc gtgtttcctt
     5221 agttggtatc gaactactag caattgaagc tcggatcgtt atcgcttccg ttgaaaccta
     5281 cttgaagtat gctgaagcag ttggtttgac ccaatcagca gcagtacctg cttaatttaa
     5341 ttaagcaagt taccaagtag gagaataacc atgactcata tttatagtta ttctcctcat
     5401 ttcccaagtt tccgacaaca aataacaagg aattaaaaaa atggcagttg aaaaaaccaa
     5461 ttcttcctcc agcttggcag aagttattga tagaatcctc gacaaaggta tcgtaattga
     5521 cgcttgggtt cgtgtttcct tagttggtat cgaactacta gcaattgaag ctcggatcgt
     5581 tatcgcttcc gttgaaacct acttgaagta tgctgaagca gttggtttga cccaatcagc
     5641 agcagtacct gcttaattta attaagctcc ataatatcag gattaattaa tcctgatatt
     5701 ggttctgctt tttttaatta tctagatatt tggaaatcaa cgcttctcaa aggatattac
     5761 aaaaaagtac gaatccttat aatgtagtac ctggagaagc aatttctgtt aggtctggga
     5821 gctcccatgg gatcgttatc ccgcggccaa gcttgctagc gctaatttac tattttttgg
     5881 agaacttcat gatttcttta atggcaaaaa tccggcaaga acatcagtca atagcagaga
     5941 aagtggctga actatctctt gagaccagag aattcttgtc cgtcacgaca gcgaaaagac
     6001 aagagcaagc tgaaaaacaa gctcaagaac tgcaagcatt ctacaaggat cttcaggaaa
     6061 caagtcagca gtttttatca gaaacagccc aagccagaat tgctcaagct gaaaaacaag
     6121 ctcaagaact gttagcattc cacaaagaac ttcaagaaac aagtcagcag tttttatcag
     6181 caacagccca agccagaatt gctcaagctg aaaaacaagc gcaagaactg ttagcatttt
     6241 atcaagaagt tcgggaaaca agtcagcagt ttttatcagc aacagcccaa gcaagaattg
     6301 ctcaagctga aaaacaagct caagaactgt tagcattcca caaagaactt caagaaacaa
     6361 gtcagcagtt tttatcagca acagccgacg caagaactgc tcaagctaag gaacagaagg
     6421 aatctctcct gaaattccgt caggatttgt ttgtgagtat ctttggtagc ggtgcggcca
     6481 acgatgaaaa ctatgcgctg gcggcctaat aaagatctaa ctattggagg ctactaaaaa
     6541 tggaaattaa aaaaattatg caagccgtga acgacttttt cggtgaacac gtagctcctc
     6601 ctcataaaat tacctcggtg gaagctactg aagatgaagg ttggagagtt attgttgaag
     6661 tcattgaaga acgagaatat atgaaaaaat acgccaaaga tgaaatgctc ggaacgtacg
     6721 agtgctttgt aaataaagaa aaagaagtca tttcattcaa acgactcgac gtcagatata
     6781 gaagcgccat tggcattgaa gcataaacga taagatggca ggaggaacgt aaaaatgacc
     6841 gtcttaacag acaaaaggaa aaaaggcagt ggagctttta tacaagatga cgagacaaaa
     6901 gaggttcttt caagagcgct gagctattta aaatccggct attccattca ttttacaggt
     6961 cctgccggcg gaggcaaaac ctctttagcg cgagcgcttg ctaaaaagag aaagcgtcct
     7021 gtaatgctga tgcacgggaa tcacgagctc aacaacaaag atttaattgg cgattttacg
     7081 ggatacacga gcaaaaaagt aatcgaccag tacgttcgtt ctgtctataa aaaagatgaa
     7141 caggtgagtg aaaactggca ggatggccga ttgcttgaag ctgtaaaaaa tggctatacg
     7201 ctgatttacg acgaatttac tcgttctaag cctgcgacga ataatatctt tctatcgata
     7261 ttagaagaag gcgtgctgcc gctgtatgga gtaaaaatga ccgatccttt tgtgcgcgtg
     7321 catcccgatt tccgcgtcat cttcacaagc aatccagctg agtatgccgg cgtatatgat
     7381 acgcaagatg cgcttctcga caggttaatt accatgttta ttgattataa agacatcgac
     7441 agagagacag cgattttaac ggagaaaacg gacgtagaag aagatgaagc gcgcacaatt
     7501 gtaacgctcg tagcaaacgt gcgaaaccgc tctggagacg aaaacagcag cggacttagc
     7561 ctgcgggctt cgcttatgat cgctaccctt gccacgcagc aagacattcc tatcgatgga
     7621 agtgacgaag attttcaaac gttatgtatc gatattttgc atcatccgct taccaaatgt
     7681 ttggatgaag aaaatgcaaa aagcaaagcc gaaaaaatca ttttagaaga atgtaagaat
     7741 atagacactg aagaaaagta aaggagcttg aaaacatgag tgaaacaaac gaaacaggta
     7801 tttatatttt tagcgccatt caaacggata aagacgaaga atttggcgcc gtggaagtag
     7861 aaggaacaaa agctgaaaca tttttgattc gctacaaaga cgcggctatg gtagcagctg
     7921 aagtaccgat gaaaatttat catcctaatc gccaaaattt attaatgcat caaaacgcag
     7981 tagcagcgat tatggacaag aacgatacgg ttattccaat cagctttggg aatgtattca
     8041 aatcaaaaga agacgtaaaa gttcttttgg aaaaccttta tccgcagttt gaaaagctgt
     8101 ttccagcgat caaaggaaaa attgaagtcg gtttaaaagt aattgggaaa aaagaatggc
     8161 ttgagaaaaa agtaaacgaa aatcctgaac ttgagaaagt atcagcatcc gtaaaaggaa
     8221 aatcagaagc agccggttat tatgagcgta ttcaacttgg aggaatggct caaaagatgt
     8281 ttacttccct gcaaaaagaa gtcaagacag atgtgttttc tccgcttgaa gaagcagcgg
     8341 aagcagcaaa agcaaatgag ccaacgggcg aaacgatgct tttaaacgcg tctttcttaa
     8401 ttaaccgaga agatgaagcg aagtttgatg aaaaagtaaa tgaagcgcat gaaaactgga
     8461 aagacaaagc cgattttcat tacagcggtc cttggcctgc ttataatttt gtgaacattc
     8521 gcctaaaagt agaagagaaa taacgtgctt cacaaattag taaccgcacc cattaacctt
     8581 gtagtgaaaa tcggcgaaaa agtacaggaa gaagctgata aacagctata tgaccttccg
     8641 acgattcagc aaaagctcat tcagcttcaa atgatgtttg agcttggtga aattccagaa
     8701 gaagcgtttc aagaaaaaga agatgaattg ttaatgaggt acgaaattgc gaaacgcaga
     8761 gaaattgaac aatgggaaga gctaacacaa aaaagaaatg aggaatccta gatgggagaa
     8821 ttactgtatt tatacggttt aattccaaca aaagaagcag cagccataga gccgtttcca
     8881 tcttataagg ggtttgacgg agaacattca ctgtacccaa ttgcgtttga tcaggtgacg
     8941 gctgtagttt ctaagctgga tgctgacacc tattcagaaa aagtgattca agaaaaaatg
     9001 gagcaggata tgagctggct gcaggaaaaa gcatttcatc atcacgaaac ggtagccgct
     9061 ttgtacgaag aatttacgat cattccatta aaattttgca ccatttataa aggtgaagaa
     9121 agtctgcagg cagctattga gattaacaaa gaaaagatag agaattcact gacgctgctt
     9181 caaggaaatg aagagtggaa tgtgaaaatt tactgtgatg atacagagct taaaaaagga
     9241 atcagcgaaa cgaatgaaag cgtgaaagcg aaaaaacaag aaattagtca cttatcacca
     9301 ggaagacagt tttttgaaaa gaaaaaaata gatcagctga ttgaaaaaga attagagctt
     9361 cacaaaaaca aagtgtgtga agagatacat gacaagctaa aagaattatc gctttatgac
     9421 tctgttaaaa agaattggag caaagacgta actggcgcag ctgaacagat ggcgtggaac
     9481 agcgtgtttc ttctcccgtc tctgcagatt actaagttcg taaacgaaat agaagagctt
     9541 cagcaaaggc ttgaaaataa aggctggaag tttgaagtga cgggaccatg gccgccctat
     9601 catttctcga gctttgcgta aagtgaggaa ttaacattat gtctcttaaa caatccatgg
     9661 agaataaaga tattgctctt attgatattt tagatgtcat tttagataaa ggagtcgcca
     9721 ttaaaggaga cttaatcatt tccatagctg gcgtcgattt agtgtatttg gatttgcggg
     9781 tgcttatttc ttcggttgaa acgcttgtgc aagcaaaaga aggaaatcac aaaccaatca
     9841 cttctgaaca atttgataaa caaaaggagg aattaatgga tgcaaccggt cagccaagca
     9901 aatggacgaa tccacttgga tcctgatcaa gctgaacaag gcttagcgca gcttgtgatg
     9961 acagttattg agctattgag gcaaatcgtt gaacgtcatg ccatgaggcg ggtggagggt
    10021 ggaacgttga cggacgaaca aattgaaaac ttaggaattg cactaatgaa cttagaagaa
    10081 aaaatggacg agttgaaaga ggtgttcggt ctggatgcag aagatttaaa tattgatctt
    10141 ggaccgctag gcagcctgct ttaagcggtc agtaggagga acagtatggc agtcgaacat
    10201 aatatgcagt caagtacgat tgtagatgtg ctcgaaaaga ttttggataa aggagtcgtt
    10261 atagcggggg acatcaccgt aggaattgca gatgtcgagc tattaacgat aaagatccgc
    10321 ttgattgtgg cttcggttga taaggcaaaa gaaatcggca tggactggtg ggaaaatgat
    10381 ccgtatctca gttcaaaagg agccaataac aaagcgctcg aagaagaaaa taaaatgctg
    10441 catgagcggt taaaaacgct tgaagaaaaa atagaaacga aacgttaaaa actgtacgct
    10501 acttaaaaaa tggagggatt tacaatggca actgaaacaa aattagataa cacacaggca
    10561 gaaaacaagg aaaataaaaa tgcggaaaac ggttcaaaag aaaagaacgg ttcaaaagca
    10621 agcaaaacaa caagcagcgg gccaatcaaa cgagcggtag caggaggcat catcggtgca
    10681 acgattggat atgtatcgac tcctgaaaat cgaaaaagtc tccttgaccg cattgataca
    10741 gacgaattaa aaagcaaagc atctgattta ggaacaaagg taaaagaaaa atcaaaaagc
    10801 agcgtggcca gcctgaaaac atctgcggga agcttgttta aaaaagataa agataaatca
    10861 aaagatgatg aagaaaacgt aaattcttct agtagcgaaa cagaagacga taacgttcaa
    10921 gagtacgacg agttaaaaga agaaaatcaa actcttcaag atcgcttatc acagcttgaa
    10981 gaaaaaatga acatgcttgt tgagcttagc ctcaataaaa atcaagacga agaagcggaa
    11041 gatacagatt ccgacgaaga agagaacgat gagaacgatg aaaacgatga aaacgagcag
    11101 gacgatgaaa acgaagaaga aacatctaag ccacgtaaaa aggataaaaa agaagctgag
    11161 gaagaagaaa gtgaaagtga cgaagacagc gaggaagaag aggaagattc tcgctcaaac
    11221 aaaaaaaata aaaaagtaaa aacagaagaa gaagacgaag atgaaagcga agaagaaaaa
    11281 aaggaagcga aaccaaaaaa gtcaacagct aaaaaatcaa aaaatacaaa agcaaagaaa
    11341 aacacggacg aagaagatga tgaagcaaca tctctttcta gtgaagacga tacaacagcc
    11401 taagacgtaa aggaggaaag aaagacatga gtacaggccc ttctttttca actaaagaca
    11461 atacgcttga atactttgtg aaagcttcta ataaacacgg cttttcactt gatatttcat
    11521 taaatgtaaa cggcgctgtg atttccggta ccatgatttc agcaaaagaa tactttgatt
    11581 acttaagcga aacgtttgaa gaaggcagtg aagtggctca ggcgctaagc gaacaattct
    11641 ctttagcaag cgaagcgagc gaatcaaacg gagaagcaga agcccatttt attcatttga
    11701 aaaatacaaa gatttactgt ggagacagta aatctactcc ttctaaaggc aaaatctttt
    11761 ggagagggaa aatagcagaa gtagacgggt ttttcttagg aaagatttct gatgcaaaat
    11821 caacgagtaa aaagagttca taagatccgg ctgctaacaa agcccgaaag gaagctgagt
    11881 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
    11941 tgaggggttt tttgctgaaa ggaggaacta tatccggat
//
