LOCUS       sequence-310165-       14078 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual bioluminescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14078)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 14078)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO1267
FEATURES             Location/Qualifiers
     source          1..14078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..346
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     289..309
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             364..384
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    400..524
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(561..566,567..584,585..591)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             645..1304
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(711..730)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             1646..1951
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     1830..1849
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(1992..2116)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3864..3929
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     terminator      4510..4762
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4795..4811)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4795..4811)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4808..4830)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4819..4835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4843..4849,4850..4867,4868..4873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4888..4909
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4943..5195)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5585..6379)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5696..5715)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6306..6325
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6400..6583)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6436..6615)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6789..6813
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7334..8125
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8371..8959
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8860..8879
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9113..9130
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9145..9285
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9211..9230)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9371..9393
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9629..9823
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9889..10962)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11391..12020)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13321..13345
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     13533..13555
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     13547..13564
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     13548..13564
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgagactttt caacaaaggg taatatccgg aaacctcctc ggattccatt gcccagctat
       61 ctgtcacttt attgtgaaga tagtggaaaa ggaaggtggc tcctacaaat gccatcattg
      121 cgataaagga aaggccatcg ttgaagatgc ctctgccgac agtggtccca aagatggacc
      181 cccacccacg aggagcatcg tggaaaaaga agacgttcca accacgtctt caaagcaagt
      241 ggattgatgt gatatctcca ctgacgtaag ggatgacgca caatcccact atccttcgca
      301 agacccttcc tctatataag gaagttcatt tcatttggag agaacacggg ggactctaga
      361 atgccaaaaa agaagagaaa ggtagaagac cccactagta caagtttgta caaaaaagct
      421 gaacgagaaa cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa
      481 cagactacat aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg
      541 ccgcattagg caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt
      601 tgagttagga tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
      661 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
      721 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
      781 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
      841 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
      901 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
      961 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     1021 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct
     1081 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     1141 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     1201 cgctggcgat tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta
     1261 atgaattaca acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct
     1321 tactaaaagc cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat
     1381 atactgatat gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac
     1441 agtgacagtt gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc
     1501 ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa
     1561 gcggaaaatc aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt
     1621 gctgacgaga acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag
     1681 ccgttatcgt ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat
     1741 ggtgatcccc ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc
     1801 ggtggtgcat atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc
     1861 ggtctccgtt atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa
     1921 cgccattaac ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc
     1981 tgcaggtcga ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt
     2041 tatgcaaaat ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt
     2101 tcttgtacaa agtggtgggc ccaatggaga atatggagaa cgacgagaat attgttgtcg
     2161 ggcctaaacc tttttacccg atagaagagg ggtctgccgg gactcagtta agaaaataca
     2221 tggaacgtta tgccaaactt ggtgccatcg cgttcacgaa tgctgtgacc ggggtagatt
     2281 actcttacgc cgaatatctt gaaaaaagct gctgtctagg gaaggcccta caaaactacg
     2341 ggcttgttgt cgacgggagg atcgcactat gttctgaaaa ttgcgaagag ttctttatcc
     2401 cagtaatcgc ggggctattt attggcgtcg gggtggcgcc caccaacgaa atatacacac
     2461 tacgagagct tgtccacagc ctagggatct ctaaacccac tatcgtattc tcatctaaga
     2521 agggactcga caaggtaatt acagtgcaaa agaccgttac gaccatcaaa acaattgtca
     2581 tactcgacag taaagtagac tacaggggct atcagtgcct cgacacgttt attaagcgaa
     2641 acaccccacc aggttttcag gcttccagct tcaagactgt ggaagttgat cgaaaggaac
     2701 aagtggcgct catcatgaac tccagcggct caacgggtct ccccaagggc gttcaactta
     2761 ctcacgaaaa cacggtaacc cgttttagcc atgcacgaga cccgatctac ggcaaccagg
     2821 taagccctgg caccgctgtc ttaacagtgg ttcctttcca tcatgggttt ggaatgttca
     2881 ccacgctagg atatctaatt tgcggcttta gagttgtgat gttgacaaag tttgatgagg
     2941 aaacttttct aaaaactcta caggactaca aatgcacata tgtgattttg gttccaacgc
     3001 ttttcgccat tcttaataaa tcagaactgt tgaacaagta cgatctcagt aacttggtgg
     3061 agatcgcttc tgggggtgcc cccctgtcta aggaagtagg agaggcagtg gctcgtcgtt
     3121 ttaacttacc gggggtccgt caaggatacg gtttgacgga aacgacatcc gccatcatca
     3181 ttacgccaga gggcgacgac aaacctggag ctagtggtaa agttgtgcct ttatttaagg
     3241 cgaaggtaat tgacctagac acgaagaaga gtctaggtcc taataggaga ggggaggtat
     3301 gcgtcaaggg acccatgctg atgaaaggtt atgttaataa cccggaagcg actaaggaat
     3361 tgattgacga ggaaggttgg ttgcatacgg gagatatagg ttattatgac gaggaaaaac
     3421 actttttcat agtagacagg ctgaagtcat tgattaaata caaagggtat caagtcccac
     3481 ccgccgagct tgaatctgtt cttcttcaac atcctagcat ttttgacgct ggggttgcgg
     3541 gagttcccga tccggttgcg ggagagctgc caggtgcagt agttgtgtta gagtccggga
     3601 agaatatgac tgaaaaggaa gtgatggact atgtggcaag tcaagtctcc aacgccaaga
     3661 gactgagagg tggcgtccga ttcgttgacg aagttcccaa ggggctaaca ggcaagattg
     3721 acgggcgtgc aatcagagag atattaaaga aacccgtggc caaaatgacg cgttctaatg
     3781 ccgccgatga ggttgcgact ggatcaggtc ttttagcgat acatccgacc gaagcgcgac
     3841 ataaacaaaa aattgtagcg ccggtcaaac aaaccctgaa tttcgactta ctcaaacttg
     3901 cgggtgacgt agagtcaaat ccagggcctg gtaccatggg tgtcaaagtg ctattcgctt
     3961 tgatctgcat cgccgttgca gaagcaaaac ccaccgaaaa taatgaggac tttaacatag
     4021 tcgcggtagc gtctaacttt gccactacgg atttggacgc tgacagaggc aagctgccgg
     4081 gtaaaaaact tccgctggaa gttctgaaag agcttgaggc taatgcacgt aaggcaggct
     4141 gcaccagagg gtgcctaatt tgcttatcac acataaagtg tacacccaag atgaagaagt
     4201 ttattcccgg aagatgccat acatacgaag gtgacaaaga gtccgcccaa ggaggtatag
     4261 gcgaagccat cgttgacata ccagagatcc caggattcaa ggacttagag cctttagagc
     4321 aattcattgc tcaggtagat ctctgcgtag actgcacaac tgggtgccta aagggcttgg
     4381 caaacgtcca atgctcagac ttattgaaga aatggttacc gcagagatgt gcaacctttg
     4441 cctcaaaaat acagggtcag gtagacaaaa taaagggagc aggtggtgac taagagctcg
     4501 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     4561 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     4621 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     4681 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     4741 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa tcatgtcata
     4801 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     4861 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattag gctgaattag
     4921 gcgcgcctat ttctgaattc ccgatctagt aacatagatg acaccgcgcg cgataattta
     4981 tcctagtttg cgcgctatat tttgttttct atcgcgtatt aaatgtataa ttgcgggact
     5041 ctaatcataa aaacccatct cataaataac gtcatgcatt acatgttaat tattacatgc
     5101 ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa gaccggcaac aggattcaat
     5161 cttaagaaac tttattgcca aatgtttgaa cgatcgggga tcatccgggt ctgtggcggg
     5221 aactccacga aaatatccga acgcagcaag atatcgcggt gcatctcggt cttgcctggg
     5281 cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga tcatattgtc gctcaggatc
     5341 gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt
     5401 tccgcagaga tcccgtgggc gaagaactcc agcatgagat ccccgcgctg gaggatcatc
     5461 cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc ggcggtggaa
     5521 tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc
     5581 ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg
     5641 cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct tcagcaatat
     5701 cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg ccacagtcga
     5761 tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca tcgccatggg
     5821 tcacgacgag atcatcgccg tcgggcatgc gcgccttgag cctggcgaac agttcggctg
     5881 gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg gcttccatcc
     5941 gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag gtagccggat
     6001 caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg gcaggagcaa
     6061 ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag tcccttcccg
     6121 cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc agccacgata
     6181 gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc ttgacaaaaa
     6241 gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag ccgattgtct
     6301 gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa cctgcgtgca
     6361 atccatcttg ttcaatcatg cgaaacgatc cagatccggt gcagattatt tggattgaga
     6421 gtgaatatga gactctaatt ggataccgag gggaatttat ggaacgtcag tggagcattt
     6481 ttgacaagaa atatttgcta gctgatagtg accttaggcg acttttgaac gcgcaataat
     6541 ggtttctgac gtatgtgctt agctcattaa actccagaaa cccgcggctg agtggctcct
     6601 tcaacgttgc ggttctgtca gttccaaacg taaaacggct tgtcccgcgt catcggcggg
     6661 ggtcataacg tgactccctt aattctccgc tcatgatgaa ttcagaaata aattcagcct
     6721 aattcggcgt taattcagta cattaaaaac gtccgcaatg tgttattaag ttgtctaagc
     6781 gtcaatttgt ttacaccaca atatatcctg ccaccagcca gccaacagct ccccgaccgg
     6841 cagctcggca caaaatcacc actcgataca ggcagcccat cagtccggga cggcgtcagc
     6901 gggagagccg ttgtaaggcg gcagactttg ctcatgttac cgatgctatt cggaagaacg
     6961 gcaactaagc tgccgggttt gaaacacgga tgatctcgcg gagggtagca tgttgattgt
     7021 aacgatgaca gagcgttgct gcctgtgatc aattcgggca cgaacccagt ggacataagc
     7081 ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac
     7141 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
     7201 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
     7261 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
     7321 aaagttaaac atcatggggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
     7381 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
     7441 cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta cggtgaccgt
     7501 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
     7561 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
     7621 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
     7681 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
     7741 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
     7801 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
     7861 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     7921 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     7981 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     8041 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
     8101 gatcaccaag gtagtcggca aataatgtct agctagaaat tcgttcaagc cgacgccgct
     8161 tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct cacagccaaa
     8221 ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc tacacaaatt
     8281 gggagatata tcatgcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     8341 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     8401 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     8461 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     8521 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     8581 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     8641 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     8701 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     8761 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     8821 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     8881 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     8941 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     9001 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     9061 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     9121 agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     9181 tatttcacac cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     9241 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca
     9301 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     9361 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     9421 aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg
     9481 ccctggtaga ttgcctggcc gtaggccagc catttttgag cggccagcgg ccgcgatagg
     9541 ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga agggtaggcg ctttttgcag
     9601 ctcttcggct gtgcgctggc cagacagtta tgcacaggcc aggcgggttt taagagtttt
     9661 aataagtttt aaagagtttt aggcggaaaa atcgcctttt ttctctttta tatcagtcac
     9721 ttacatgtgt gaccggttcc caatgtacgg ctttgggttc ccaatgtacg ggttccggtt
     9781 cccaatgtac ggctttgggt tcccaatgta cgtgctatcc acaggaaaga gaccttttcg
     9841 acctttttcc cctgctaggg caatttgccc tagcatctgc tccgtacatt aggaaccggc
     9901 ggatgcttcg ccctcgatca ggttgcggta gcgcatgact aggatcgggc cagcctgccc
     9961 cgcctcctcc ttcaaatcgt actccggcag gtcatttgac ccgatcagct tgcgcacggt
    10021 gaaacagaac ttcttgaact ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa
    10081 ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat
    10141 gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc acgtccgggt tcttgccttc
    10201 tgtgatctcg cggtacatcc aatcagctag ctcgatctcg atgtactccg gccgcccggt
    10261 ttcgctcttt acgatcttgt agcggctaat caaggcttca ccctcggata ccgtcaccag
    10321 gcggccgttc ttggccttct tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat
    10381 gcaggtttct accaggtcgt ctttctgctt tccgccatcg gctcgccggc agaacttgag
    10441 tacgtccgca acgtgtggac ggaacacgcg gccgggcttg tctcccttcc cttcccggta
    10501 tcggttcatg gattcggtta gatgggaaac cgccatcagt accaggtcgt aatcccacac
    10561 actggccatg ccggccggcc ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg
    10621 gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg aaaacggcca cgtccatgat
    10681 gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga acgaaaaaat ctggttgctc
    10741 gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct gccggcggtt gccgggattc
    10801 tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat
    10861 gcgttgccgc tgggcggcct gcgcggcctt caacttctcc accaggtcat cacccagcgc
    10921 cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc acgccgattc ctcgggcttg
    10981 ggggttccag tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg
    11041 cccgttcctc cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc
    11101 atgccgcctc ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc
    11161 tggtagctgc gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc
    11221 gtacatcttc agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc
    11281 tggcgtgtct gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc
    11341 ggcacttagc gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt
    11401 tttgatttaa tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct
    11461 gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata
    11521 cgggactcaa gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgatg
    11581 cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt gtagccttcc atccgtgacc
    11641 tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg cccatatgtc gtaagggctt
    11701 ggctgcaccg gaatcagcac gaagtcggct gccttgatcg cggacacagc caagtccgcc
    11761 gcctggggcg ctccgtcgat cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg
    11821 tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt gatcttcccg cacggccgcc
    11881 caatcgcggg cactgccctg gggatcggaa tcgactaaca gaacatcggc cccggcgagt
    11941 tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta
    12001 agtacagcga taaccttcat gcgttcccct tgcgtatttg tttatttact catcgcatca
    12061 tatacgcagc gaccgcatga cgcaagctgt tttactcaaa tacacatcac ctttttagac
    12121 ggcggcgctc ggtttcttca gcggccaagc tggccggcca ggccgccagc ttggcatcag
    12181 acaaaccggc caggatttca tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca
    12241 cgtacccggc cgcgatcatc tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct
    12301 ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt tcattctcgg cggccgccag
    12361 ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg
    12421 cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc gagcgatgca cgccaagcag
    12481 tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat
    12541 ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg cctcgggcct tggcggcctc
    12601 gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg aactcggcaa tgccggcgaa
    12661 cacggtcaac accatgcggc cggccggcgt ggtggtgtcg gcccacggct ctgccaggct
    12721 acgcaggccc gcgccggcct cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct
    12781 gcgggccagg cggtctagcc tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag
    12841 catcctggcc agctccgggc ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt
    12901 ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc aagtcctggt cgtcggtgct
    12961 gacgcgggca tagcccagca ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg
    13021 gttctagtcg caagtattct actttatgcg actaaaacac gcgacaagaa aacgccagga
    13081 aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga
    13141 agacggctgc actgaacgtc agaagccgac tgcactatag cagcggaggg gttggatcaa
    13201 agtactttga tcccgagggg aaccctgtgg ttggcatgca catacaaatg gacgaacgga
    13261 taaacctttt cacgcccttt taaatatccg attattctaa taaacgctct tttctcttag
    13321 gtttacccgc caatatatcc tgtcaaacac tgatagttta aactgaaggc gggaaacgac
    13381 aatctgatcc aagctcaagc tgctctagca ttcgccattc aggctgcgca actgttggga
    13441 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
    13501 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
    13561 cagtgccaag cttgcatgcc tgcaggtccc cagattagcc ttttcaattt cagaaagaat
    13621 gctaacccac agatggttag agaggcttac gcagcaggtc tcatcaagac gatctacccg
    13681 agcaataatc tccaggaaat caaatacctt cccaagaagg ttaaagatgc agtcaaaaga
    13741 ttcaggacta actgcatcaa gaacacagag aaagatatat ttctcaagat cagaagtact
    13801 attccagtat ggacgattca aggcttgctt cacaaaccaa ggcaagtaat agagattgga
    13861 gtctctaaaa aggtagttcc cactgaatca aaggccatgg agtcaaagat tcaaatagag
    13921 gacctaacag aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc
    13981 aatgacaaga agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa
    14041 aatatcaaag atacagtctc agaagaccaa agggcaat
//
