LOCUS       sequence-31017-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Greggio E, Jain S, Kingsbury A, Bandopadhyay R, Lewis P, Kaganovich 
            A, van der Brug MP, Beilina A, Blackinton J, Thomas KJ, Ahmad R, 
            Miller DW, Kesavapany S, Singleton A, Lees A, Harvey RJ, Harvey K, 
            Cookson MR
  TITLE     Kinase activity is required for the toxic effects of mutant 
            LRRK2/dardarin.
  JOURNAL   Neurobiol Dis. 2006 Aug . 23(2):329-41.
  PUBMED    16750377
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neurobiol 
            Dis. 2006 Aug . 23(2):329-41."
COMMENT     Sequence Label: pDEST53-LRRK2-R1441C
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(96..98,99..101,102..812)
                     /codon_start=1
                     /product="Cycle 3 GFP (Crameri et al., 1996)"
                     /label=Cycle 3 GFP
                     /note="mammalian codon-optimized"
                     /translation="MASKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(129..157)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     752..772
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag acaccatggc cagcaaagga gaagaacttt
      121 tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt
      181 ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt aaatttattt
      241 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc tcttatggtg
      301 ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc aagagtgcca
      361 tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg aactacaaga
      421 cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag ttaaaaggta
      481 ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac tataactcac
      541 acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac ttcaaaattc
      601 gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa aatactccaa
      661 ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa tctgcccttt
      721 cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta actgctgctg
      781 ggattacaca tggcatggat gagctctaca aaagcggttc cgga
//
