LOCUS       sequence-310170-       14073 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway cloning compatible dual fluorescent ratiometric binary 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14073)
  AUTHORS   Khosla A, Rodriguez-Furlan C, Kapoor S, Van Norman JM, Nelson DC
  TITLE     A series of dual-reporter vectors for ratiometric analysis of 
            protein abundance in plants.
  JOURNAL   Plant Direct. 2020 Jun 21;4(6):e00231. doi: 10.1002/pld3.231. 
            eCollection 2020 Jun.
  PUBMED    32582876
REFERENCE   2  (bases 1 to 14073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Direct."; date: "2020-06-21"; volume: "4(6):e00231. doi"; pages: " 
            10.1002/pld3.231. eCollection 2020 Jun"
COMMENT     Sequence Label: pRATIO2214
FEATURES             Location/Qualifiers
     source          1..14073
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1614..1634
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    1650..1774
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1811..1816,1817..1834,1835..1841)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1895..2554
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1961..1980)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2896..3201
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     3080..3099
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3242..3366)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             4166..4231
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     CDS             4961..4981
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      5010..5262
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(5295..5311)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5295..5311)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5308..5330)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5319..5335
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5343..5349,5350..5367,5368..5373))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5388..5409
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(5443..5695)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(6085..6879)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6196..6215)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6806..6825
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6900..7083)
                     /function="NOS promoter"
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     promoter        complement(6936..7115)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7289..7313
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7834..8625
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8871..9459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9360..9379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9613..9630
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9645..9785
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9711..9730)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9871..9893
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10129..10323
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10389..11462)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11891..12520)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    13821..13845
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     14033..14055
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     14047..14064
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     14048..14064
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgacgtcgta tgatcgcgaa gcacccaccc taagccacat tttaatcctt ctgttggcca
       61 tgccccatca aagttgcact taacccaaga ttgtggtgga gcttcccatg tttctcgtct
      121 gtcccgacgg tgttgtggtt ggtgctttcc ttacattctg agcctctttc cttctaatcc
      181 actcatctgc atcttcttgt gtccttacta atacctcatt ggttccaaat tccctccctt
      241 taagcaccag ctcgtttctg ttcttccaca gcctcccaag tatccaaggg actaaagcct
      301 ccacattctt cagatcagga tattcttgtt taagatgttg aactctatgg aggtttgtat
      361 gaactgatga tctaggaccg gataagttcc cttcttcata gcgaacttat tcaaagaatg
      421 ttttgtgtat cattcttgtt acattgttat taatgaaaaa atattattgg tcattggact
      481 gaacacgagt gttaaatatg gaccaggccc caaataagat ccattgatat atgaattaaa
      541 taacaagaat aaatcgagtc accaaaccac ttgccttttt taacgagact tgttcaccaa
      601 cttgatacaa aagtcattat cctatgcaaa tcaataatca tacaaaaata tccaataaca
      661 ctaaaaaatt aaaagaaatg gataatttca caatatgtta tacgataaag aagttacttt
      721 tccaagaaat tcactgattt tataagccca cttgcattag ataaatggca aaaaaaaaca
      781 aaaaggaaaa gaaataaagc acgaagaatt ccagaaaata cgaaatacgc ttcaatgcag
      841 tgggacccac ggttcaatta ttgccaattt tcagctccac cgtatattta aaaaataaaa
      901 cgataatgct aaaaaaatat aaatcgtaac gatcgttaaa tctcaacggc tggatcttat
      961 gacgaccgtt agaaattgtg gttgtcgacg agtcagtaat aaacggcgtc aaagtggttg
     1021 cagccggcac acacgagtcg tgtttatcaa ctcaaagcac aaatactttt cctcaaccta
     1081 aaaataaggc aattagccaa aaacaacttt gcgtgtaaac aacgctcaat acacgtgtca
     1141 ttttattatt agctattgct tcaccgcctt agctttctcg tgacctagtc gtcctcgtct
     1201 tttcttcttc ttcttctata aaacaatacc caaagagttc ttcttcttca caattcagat
     1261 ttcaatttct caaaatctta aaaactttct ctcaattctc tctaccgtga tcaaggtaaa
     1321 tttctgtgtt ccttattctc tcaaaatctt cgattttgtt ttcgttcgat cccaatttcg
     1381 tatatgttct ttggtttaga ttctgttaat cttagatcga agacgatttt ctgggtttga
     1441 tcgttagata tcatcttaat tctcgattag ggtttcatag atatcatccg atttgttcaa
     1501 ataatttgag ttttgtcgaa taattactct tcgatttgtg atttctatcc agatctggtg
     1561 ttagtttcta gtttgtgcga tcgaatttgt cctcgagagg ggactctaga atgccaaaaa
     1621 agaagagaaa ggtagaagac cccactagta caagtttgta caaaaaagct gaacgagaaa
     1681 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     1741 aatactgtaa aacacaacat atccagtcac tatggcggcc cctcgagggg ccgcattagg
     1801 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga
     1861 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac
     1921 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc
     1981 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa
     2041 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc
     2101 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca
     2161 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata
     2221 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga
     2281 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc
     2341 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc
     2401 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat
     2461 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca
     2521 acagtactgc gatgagtggc agggcggggc gtaaagatct ggatccggct tactaaaagc
     2581 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat
     2641 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt
     2701 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa
     2761 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc
     2821 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga
     2881 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt
     2941 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc
     3001 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat
     3061 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt
     3121 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac
     3181 ctgatgttct ggggaatata aatgtcaggc tcccttatac acagccagtc tgcaggtcga
     3241 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat
     3301 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa
     3361 agtggtgggc ccaatggtgt ccaagggcga agctgtgatt aaagaattta tgcgttttaa
     3421 ggtccacatg gaaggatcca tgaatggaca tgaattcgaa atcgaggggg agggcgaggg
     3481 ccgaccttat gagggtacac agaccgcgaa gctaaaggta accaagggag gcccactacc
     3541 ctttagttgg gacatactca gcccgcagtt tatgtatggc agtcgtgcct ttataaagca
     3601 cccagcggat ataccagact actacaaaca gtccttccca gaggggttta aatgggagcg
     3661 agtgatgaac tttgaggacg gtggtgctgt gacggtaact caggacacat cactcgaaga
     3721 cgggactctt atttacaaag taaaactgag agggaccaat tttccaccag atggccccgt
     3781 catgcaaaaa aagacgatgg gttgggaagc atctacagaa cgactctatc ccgaggacgg
     3841 cgtcctcaaa ggcgacatta aaatggcctt gaggttgaaa gacggtggac gttacttggc
     3901 ggacttcaaa acaacttata aagcaaaaaa gccagtgcaa atgcctggag cgtacaatgt
     3961 agatagaaaa cttgatataa cgtcacataa tgaggactat accgtggttg agcaatacga
     4021 gaggtcagaa ggacgacaca gcaccggcgg tatggacgag ttgtacaaga cgcgttctaa
     4081 tgccgccgat gaggttgcga ctggatcagg tcttttagcg atacatccga ccgaagcgcg
     4141 acataaacaa aaaattgtag cgccggtcaa acaaaccctg aatttcgact tactcaaact
     4201 tgcgggtgac gtagagtcaa atccagggcc tggtaccatg gtatcaaagg gcgaggaatt
     4261 gtttacgggc gttgtaccaa tcctggtcga actggacggc gacgttaacg gacataaatt
     4321 ttcagtaagc ggggaaggtg agggggatgc aacgtacggg aagctgactt taaaatttat
     4381 atgcaccaca gggaagctac ctgtgccctg gcctacgttg gtaacgaccc tgacatgggg
     4441 tgtacagtgc tttgcaagat acccagacca catgaagcag cacgatttct ttaagtctgc
     4501 catgcctgag ggttatgtac aagagcgtac aatttttttt aaagatgatg gcaactataa
     4561 gacccgtgcc gaggtaaaat ttgaggggga tactctagtg aatagaatcg aattgaaggg
     4621 tatagatttc aaggaggacg gaaacatctt ggggcataaa ctagagtaca acgccatcag
     4681 tgacaacgta tatattacag cggacaaaca aaaaaacggc ataaaagcaa actttaagat
     4741 tcgtcacaac atcgaggacg gcagtgtgca gctggcagac cactaccagc aaaatacacc
     4801 aataggagat ggacctgtat tactgccaga caaccactac ttatccactc agagtaaatt
     4861 aagtaaggac cctaacgaga aaagggatca catggtccta ctagagttcg tgacggcagc
     4921 cggaattact ttaggcatgg acgaactgta caaggaattc ccaaaaaaga agagaaaggt
     4981 agaagacccc taagagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     5041 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     5101 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     5161 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     5221 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaattg gttccggaac
     5281 caattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     5341 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     5401 ctcacattag gctgaattag gcgcgcctat ttctgaattc ccgatctagt aacatagatg
     5461 acaccgcgcg cgataattta tcctagtttg cgcgctatat tttgttttct atcgcgtatt
     5521 aaatgtataa ttgcgggact ctaatcataa aaacccatct cataaataac gtcatgcatt
     5581 acatgttaat tattacatgc ttaacgtaat tcaacagaaa ttatatgata atcatcgcaa
     5641 gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa cgatcgggga
     5701 tcatccgggt ctgtggcggg aactccacga aaatatccga acgcagcaag atatcgcggt
     5761 gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt gatgtggacg ccgggcccga
     5821 tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc cgttgctgtc gtaatgatat
     5881 cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc gaagaactcc agcatgagat
     5941 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt
     6001 catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg
     6061 tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat
     6121 gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc
     6181 gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac
     6241 acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg
     6301 caagcaggca tcgccatggg tcacgacgag atcatcgccg tcgggcatgc gcgccttgag
     6361 cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc
     6421 gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc
     6481 gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga
     6541 tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa
     6601 tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc
     6661 cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga
     6721 caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc
     6781 atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc
     6841 ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc cagatccggt
     6901 gcagattatt tggattgaga gtgaatatga gactctaatt ggataccgag gggaatttat
     6961 ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg accttaggcg
     7021 acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa actccagaaa
     7081 cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg taaaacggct
     7141 tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc tcatgatgaa
     7201 ttcagaaata aattcagcct aattcggcgt taattcagta cattaaaaac gtccgcaatg
     7261 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     7321 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     7381 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     7441 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     7501 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc aattcgggca
     7561 cgaacccagt ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc
     7621 tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt
     7681 ttcatggctt gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag
     7741 caagcgcgtt acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag
     7801 cagggcagtc gccctaaaac aaagttaaac atcatggggg aagcggtgat cgccgaagta
     7861 tcgactcaac tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg
     7921 gccgtacatt tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat
     7981 ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac
     8041 cttttggaaa cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc
     8101 attgttgtgc acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt
     8161 ggagaatggc agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt
     8221 gatctggcta tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg
     8281 gcggaggaac tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc
     8341 ttaacgctat ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg
     8401 ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc
     8461 gactgggcaa tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag
     8521 gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt
     8581 gtccactacg tgaaaggcga gatcaccaag gtagtcggca aataatgtct agctagaaat
     8641 tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca
     8701 cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca
     8761 taataagccc tacacaaatt gggagatata tcatgcatga ccaaaatccc ttaacgtgag
     8821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     8881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     8941 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     9001 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
     9061 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     9121 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     9181 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     9241 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     9301 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     9361 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     9421 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     9481 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     9541 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     9601 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
     9661 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
     9721 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
     9781 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
     9841 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
     9901 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
     9961 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
    10021 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
    10081 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
    10141 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
    10201 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
    10261 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
    10321 acaggaaaga gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
    10381 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
    10441 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
    10501 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
    10561 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
    10621 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
    10681 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
    10741 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
    10801 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
    10861 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
    10921 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
    10981 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
    11041 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
    11101 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
    11161 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
    11221 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
    11281 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
    11341 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
    11401 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
    11461 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag acaacccagc
    11521 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
    11581 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
    11641 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
    11701 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
    11761 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
    11821 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
    11881 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
    11941 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
    12001 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
    12061 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    12121 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    12181 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    12241 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    12301 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    12361 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    12421 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    12481 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    12541 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    12601 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    12661 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    12721 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    12781 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    12841 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    12901 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    12961 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    13021 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    13081 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    13141 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    13201 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    13261 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    13321 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    13381 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    13441 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    13501 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    13561 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    13621 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    13681 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    13741 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
    13801 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
    13861 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
    13921 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
    13981 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
    14041 cgacgttgta aaacgacggc cagtgccaag ctt
//
