LOCUS       sequence-310233-        9957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9957)
  AUTHORS   Krupka N, Strappe P, Gotz J, Ittner LM
  TITLE     Gateway-compatible lentiviral transfer vectors for ubiquitin 
            promoter driven expression of fluorescent fusion proteins.
  JOURNAL   Plasmid. 2010 May;63(3):155-60. doi: 10.1016/j.plasmid.2010.01.002. 
            Epub 2010 Feb 2.
  PUBMED    20132838
REFERENCE   2  (bases 1 to 9957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.plasmid.2010.01.002"; journalName: "Plasmid"; date: 
            "2010-05"; volume: "63"; issue: "3"; pages: "155-60"
COMMENT     Sequence Label: pLVU/RED
FEATURES             Location/Qualifiers
     source          1..9957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    1272..1396
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1421..1426,1427..1444,1445..1451)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1505..2164
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1571..1590)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2506..2811
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2690..2709
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(2852..2976)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             3029..3706
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     3427..3446
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(3743..3763)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3748..4077
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3928..4063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        4125..4172
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4191..4589
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     LTR             4676..4909
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4981..5102
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5018..5037)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5072..5091
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5142..5277
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5297..5316)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5298..5316)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5326..5343)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5326..5342)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5335..5357)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5484..5939
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5571..5590)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5781..5802
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5965..6069
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6070..6138,6139..6930)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6288..6307)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7101..7689
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7590..7609
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7843..7860
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7977..7998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8013..8018,8019..8036,8037..8043)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8051..8067
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8056..8078
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8075..8091
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8075..8091
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8110..8130
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8112..8130
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8158..8384
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8385..8565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8612..8737
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9230..9463
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9648..9692
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg gtaccgagct cggatccact agtccagtgt ggtggaattc
     1261 tgcagatatc aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
     1321 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
     1381 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
     1441 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
     1501 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
     1561 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
     1621 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
     1681 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga
     1741 cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc atgagcaaac
     1801 tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt ttctacacat
     1861 atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta aagggtttat
     1921 tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa
     1981 cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat attatacgca
     2041 aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt
     2101 ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc agggcggggc
     2161 gtaaagatct ggatccggct tactaaaagc cagataacag tatgcgtatt tgcgcgctga
     2221 tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa aaagaggtat
     2281 gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt gctcaaggca
     2341 tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa gcccgtcgtc
     2401 tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc gcccggttta
     2461 ttgaaatgaa cggctctttt gctgacgaga acaggggctg gtgaaatgca gtttaaggtt
     2521 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
     2581 gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
     2641 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
     2701 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
     2761 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
     2821 tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt gtgttttaca
     2881 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
     2941 ttacgtttct cgttcagctt tcttgtacaa agtggttgat atccagcaca gtggcggccg
     3001 ctcgagtcta gagggcccgc ggttcgaaat ggacaacacc gaggacgtca tcaaggagtt
     3061 catgcagttc aaggtgcgca tggagggctc cgtgaacggc cactacttcg agatcgaggg
     3121 cgagggcgag ggcaagccct acgagggcac ccagaccgcc aagctgcagg tgaccaaggg
     3181 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggc
     3241 ctacgtgaag caccccgccg acatccccga ctacatgaag ctgtccttcc ccgagggctt
     3301 cacctgggag cgctccatga acttcgagga cggcggcgtg gtggaggtgc agcaggactc
     3361 ctccctgcag gacggcacct tcatctacaa ggtgaagttc aagggcgtga acttccccgc
     3421 cgacggcccc gtaatgcaga agaagactgc cggctgggag ccctccaccg agaagctgta
     3481 cccccaggac ggcgtgctga agggcgagat ctcccacgcc ctgaagctga aggacggcgg
     3541 ccactacacc tgcgacttca agaccgtgta caaggccaag aagcccgtgc agctgcccgg
     3601 caaccactac gtggactcca agctggacat caccaaccac aacgaggact acaccgtggt
     3661 ggagcagtac gagcacgccg aggcccgcca ctccggctcc cagtaacgta ccggttagta
     3721 atgagtttgg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     3781 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     3841 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     3901 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     3961 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     4021 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     4081 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     4141 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagc
     4201 ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc aacagcatcc
     4261 ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc cgcatcttca
     4321 ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg gtgctgggca
     4381 ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga aatgagaaca
     4441 ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg catcctggga
     4501 tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt cgtgaattgc
     4561 tgccctctgg ttatgtgtgg gagggctaag cacaattcga gctcggtacc tttaagacca
     4621 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     4681 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     4741 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     4801 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     4861 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     4921 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     4981 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     5041 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     5101 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     5161 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     5221 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     5281 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     5341 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     5401 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     5461 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     5521 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     5581 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     5641 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     5701 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     5761 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     5821 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     5881 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     5941 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6001 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6061 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6121 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6181 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6241 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6301 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6361 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6421 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6481 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6541 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6601 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6661 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6721 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     6781 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     6841 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6901 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6961 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7021 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7081 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7141 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7201 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     7261 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7321 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7381 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7441 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7501 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7561 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7621 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7681 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7741 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     7801 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     7861 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7921 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7981 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     8041 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     8101 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt
     8161 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     8221 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     8281 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     8341 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     8401 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8461 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8521 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8581 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8641 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8701 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     8761 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     8821 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     8881 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     8941 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9001 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9061 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     9121 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     9181 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     9241 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     9301 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     9361 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     9421 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     9481 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     9541 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     9601 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     9661 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     9721 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     9781 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     9841 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     9901 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggatct
//
