LOCUS       sequence-310247-       10619 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK15 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10619)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK15_IDG-K
FEATURES             Location/Qualifiers
     source          1..10619
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    133..249
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    283..303
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        303..1514
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1351..1370
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1518..1538)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1539..1548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1551..2321
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2328..2369
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2370..2394
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3556..3580)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3610..4198
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3663..3683)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4081..4092)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4212..4711
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4247..4270)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4644..4663
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4732..5331
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4732..4751)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5228..5248
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5469..5702
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5774..5895
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5811..5830)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5865..5884
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5935..6070
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6090..6109)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6091..6109)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6119..6136)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6119..6135)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6128..6150)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6277..6732
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6364..6383)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6574..6595
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6758..6862
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6863..6931,6932..7723)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7894..8482
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8383..8402
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8636..8653
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8770..8791
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8806..8811,8812..8829,8830..8836)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8844..8860
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8849..8871
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8868..8884
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8868..8884
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8903..8923
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8905..8923
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8951..9177
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9178..9358
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9405..9530
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10023..10256
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10441..10485
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
       61 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
      121 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
      181 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt
      241 caaaatttta tcgtccagtg tggtggaatt ctgcagatat cacaactttg tatagaaaag
      301 ttggcctccg cgccgggttt tggcgcctcc cgcgggcgcc cccctcctca cggcgagcgc
      361 tgccacgtca gacgaagggc gcagcgagcg tcctgatcct tccgcccgga cgctcaggac
      421 agcggcccgc tgctcataag actcggcctt agaaccccag tatcagcaga aggacatttt
      481 aggacgggac ttgggtgact ctagggcact ggttttcttt ccagagagcg gaacaggcga
      541 ggaaaagtag tcccttctcg gcgattctgc ggagggatct ccgtggggcg gtgaacgccg
      601 atgattatat aaggacgcgc cgggtgtggc acagctagtt ccgtcgcagc cgggatttgg
      661 gtcgcggttc ttgtttgtgg atcgctgtga tcgtcacttg gtgagtagcg ggctgctggg
      721 ctggccgggg ctttcgtggc cgccgggccg ctcggtggga cggaagcgtg tggagagacc
      781 gccaagggct gtagtctggg tccgcgagca aggttgccct gaactggggg ttggggggag
      841 cgcagcaaaa tggcggctgt tcccgagtct tgaatggaag acgcttgtga ggcgggctgt
      901 gaggtcgttg aaacaaggtg gggggcatgg tgggcggcaa gaacccaagg tcttgaggcc
      961 ttcgctaatg cgggaaagct cttattcggg tgagatgggc tggggcacca tctggggacc
     1021 ctgacgtgaa gtttgtcact gactggagaa ctcggtttgt cgtctgttgc gggggcggca
     1081 gttatggcgg tgccgttggg cagtgcaccc gtacctttgg gagcgcgcgc cctcgtcgtg
     1141 tcgtgacgtc acccgttctg ttggcttata atgcagggtg gggccacctg ccggtaggtg
     1201 tgcggtaggc ttttctccgt cgcaggacgc agggttcggg cctagggtag gctctcctga
     1261 atcgacaggc gccggacctc tggtgagggg agggataagt gaggcgtcag tttctttggt
     1321 cggttttatg tacctatctt cttaagtagc tgaagctccg gttttgaact atgcgctcgg
     1381 ggttggcgag tgtgttttgt gaagtttttt aggcaccttt tgaaatgtaa tcatttgggt
     1441 caatatgtaa ttttcagtgt tagactagta aattgtccgc taaattctgg ccgtttttgg
     1501 cttttttgtt agacggccaa cttttgtata caaagtgggc caccatggga atcccgctgc
     1561 tgaacgctaa acagattctg ggacagctgg acggcgggag cgtggcagtc ctgcctgtgg
     1621 tcgactccac caatcagtac ctgctggatc gaatcggcga gctgaagagt ggggatgctt
     1681 gcattgcaga atatcagcag gcagggagag gaagcagagg gaggaaatgg ttctctcctt
     1741 ttggagctaa cctgtacctg agtatgtttt ggcgcctgaa gcggggacca gcagcaatcg
     1801 gcctgggccc ggtcatcgga attgtcatgg cagaagcgct gcgaaagctg ggagcagaca
     1861 aggtgcgagt caaatggccc aatgacctgt atctgcagga tagaaagctg gcaggcatcc
     1921 tggtggagct ggccggaata acaggcgatg ctgcacagat cgtcattggc gccgggatta
     1981 acgtggctat gaggcgcgtg gaggaaagcg tggtcaatca gggctggatc acactgcagg
     2041 aagcagggat taacctggac aggaatactc tggccgctat gctgatccga gagctgcggg
     2101 cagccctgga actgttcgag caggaaggcc tggctccata tctgtcacgg tgggagaagc
     2161 tggataactt catcaataga cccgtgaagc tgatcattgg ggacaaagag attttcggga
     2221 ttagccgggg gattgataaa cagggagccc tgctgctgga acaggacgga gttatcaaac
     2281 cctggatggg cggagaaatc agtctgcggt ctgccgaaaa ggctagcggc aagcccatcc
     2341 ccaaccccct gctgggcctg gacagcacca caagtttgta caaaaaagca ggctccacca
     2401 tgacttcatt tccccccagg ggacttcaag ctgcccgtgc ccagaagttc aagagtaaaa
     2461 ggccacggag taacagtgat tgttttcagg aagaggatct gaggcagggt tttcagtgga
     2521 ggaagagcct cccttttggg gcagcctcat cttacttgaa cttggagaag ctgggtgaag
     2581 gctcttatgc gacagtttac aaggggatta gcagaataaa tggacaacta gtggctttaa
     2641 aagtcatcag catgaatgca gaggaaggag tcccatttac agctatccga gaagcttctc
     2701 tcctgaaggg tttgaaacat gccaatattg tgctcctgca tgacataatc cacaccaaag
     2761 agacactgac attcgttttt gaatacatgc acacagacct ggcccagtat atgtctcagc
     2821 atccaggagg gcttcatcct cataatgtca gacttttcat gtttcaactt ttgcggggcc
     2881 tggcgtacat ccaccaccaa cacgttcttc acagggacct gaaacctcag aacttactca
     2941 tcagtcacct gggagagctc aaactggctg attttggtct tgcccgggcc aagtccattc
     3001 ccagccagac atactcttca gaagtcgtga ccctctggta ccggccccct gatgctttgc
     3061 tgggagccac tgaatattcc tctgagctgg acatatgggg tgcaggctgc atctttattg
     3121 aaatgttcca gggtcaacct ttgtttcctg gggtttccaa catccttgaa cagctggaga
     3181 aaatctggga ggtgctggga gtccctacag aggatacttg gccgggagtc tccaagctac
     3241 ctaactacaa tccagaatgg ttcccactgc ctacgcctcg aagccttcat gttgtctgga
     3301 acaggctggg cagggttcct gaagctgaag acctggcctc ccagatgcta aaaggctttc
     3361 ccagagaccg cgtctccgcc caggaagcac ttgttcatga ttatttcagc gccctgccat
     3421 ctcagctgta ccagcttcct gatgaggagt ctttgtttac agtttcagga gtgaggctaa
     3481 agccagaaat gtgtgacctt ttggcctcct accagaaagg tcaccaccca gcccagttta
     3541 gcaaatgctg gtaggaccca gctttcttgt acaaagtggt gatatccagc acagtggcgg
     3601 ccgctcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     3661 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     3721 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     3781 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     3841 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     3901 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     3961 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca
     4021 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     4081 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     4141 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcct
     4201 ggaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat gcgctttagc
     4261 agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac attccacatc
     4321 caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac cttctactcc
     4381 tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat
     4441 ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc aatggaagcg
     4501 ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct gggctcagag
     4561 gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg gggcgggcgc
     4621 ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct
     4681 gttctcctct tcctcatctc cgggcctttc gacctgcagc ccaagcttac catgaccgag
     4741 tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc
     4801 gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc
     4861 gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag
     4921 gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa
     4981 gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg
     5041 gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg
     5101 ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc
     5161 gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc
     5221 gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag
     5281 gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg acgcccgccc
     5341 cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatctcg agggcccggt
     5401 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     5461 ggggggactg gaagggctag ctcactccca acgaagacaa gatctgcttt ttgcttgtac
     5521 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     5581 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     5641 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     5701 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     5761 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     5821 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     5881 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     5941 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6001 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6061 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     6121 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     6181 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     6241 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     6301 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6361 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6421 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6481 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6541 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     6601 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     6661 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     6721 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6781 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6841 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6901 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6961 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     7021 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     7081 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     7141 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7201 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7261 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7321 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7381 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7441 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7501 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     7561 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     7621 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     7681 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     7741 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7801 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7861 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7921 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7981 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     8041 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     8101 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     8161 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     8221 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     8281 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     8341 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     8401 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     8461 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     8521 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     8581 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     8641 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     8701 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     8761 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     8821 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     8881 tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct
     8941 gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt
     9001 tagcaacatg ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg
     9061 tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc
     9121 actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg
     9181 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     9241 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     9301 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     9361 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     9421 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     9481 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     9541 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     9601 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     9661 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     9721 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     9781 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9841 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     9901 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9961 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
    10021 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
    10081 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
    10141 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
    10201 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
    10261 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
    10321 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
    10381 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
    10441 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
    10501 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
    10561 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacc
//
