LOCUS       sequence-310263-       11036 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of ADCK1 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11036)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 11036)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-ADCK1_IDG-K
FEATURES             Location/Qualifiers
     source          1..11036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    139..255
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    289..309
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        309..1520
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1357..1376
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1524..1544)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1545..1554
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1557..2327
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2334..2375
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2376..2400
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3979..4003)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4033..4621
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4086..4106)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4504..4515)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4635..5134
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4670..4693)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5067..5086
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5155..5754
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5155..5174)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5651..5671
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5892..6125
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6197..6318
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6234..6253)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6288..6307
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6358..6493
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6513..6532)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6514..6532)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6542..6559)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6542..6558)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6551..6573)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6700..7155
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6787..6806)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6997..7018
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7181..7285
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7286..7354,7355..8146)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      8317..8905
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8806..8825
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9059..9076
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9193..9214
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9229..9234,9235..9252,9253..9259)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9267..9283
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9272..9294
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9291..9307
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9291..9307
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9326..9346
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9328..9346
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9374..9600
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9601..9781
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9828..9953
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10446..10679
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10864..10908
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
       61 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
      121 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
      181 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
      241 aaaattcaaa attttatcgt ccagtgtggt ggaattctgc agatatcaca actttgtata
      301 gaaaagttgg cctccgcgcc gggttttggc gcctcccgcg ggcgcccccc tcctcacggc
      361 gagcgctgcc acgtcagacg aagggcgcag cgagcgtcct gatccttccg cccggacgct
      421 caggacagcg gcccgctgct cataagactc ggccttagaa ccccagtatc agcagaagga
      481 cattttagga cgggacttgg gtgactctag ggcactggtt ttctttccag agagcggaac
      541 aggcgaggaa aagtagtccc ttctcggcga ttctgcggag ggatctccgt ggggcggtga
      601 acgccgatga ttatataagg acgcgccggg tgtggcacag ctagttccgt cgcagccggg
      661 atttgggtcg cggttcttgt ttgtggatcg ctgtgatcgt cacttggtga gtagcgggct
      721 gctgggctgg ccggggcttt cgtggccgcc gggccgctcg gtgggacgga agcgtgtgga
      781 gagaccgcca agggctgtag tctgggtccg cgagcaaggt tgccctgaac tgggggttgg
      841 ggggagcgca gcaaaatggc ggctgttccc gagtcttgaa tggaagacgc ttgtgaggcg
      901 ggctgtgagg tcgttgaaac aaggtggggg gcatggtggg cggcaagaac ccaaggtctt
      961 gaggccttcg ctaatgcggg aaagctctta ttcgggtgag atgggctggg gcaccatctg
     1021 gggaccctga cgtgaagttt gtcactgact ggagaactcg gtttgtcgtc tgttgcgggg
     1081 gcggcagtta tggcggtgcc gttgggcagt gcacccgtac ctttgggagc gcgcgccctc
     1141 gtcgtgtcgt gacgtcaccc gttctgttgg cttataatgc agggtggggc cacctgccgg
     1201 taggtgtgcg gtaggctttt ctccgtcgca ggacgcaggg ttcgggccta gggtaggctc
     1261 tcctgaatcg acaggcgccg gacctctggt gaggggaggg ataagtgagg cgtcagtttc
     1321 tttggtcggt tttatgtacc tatcttctta agtagctgaa gctccggttt tgaactatgc
     1381 gctcggggtt ggcgagtgtg ttttgtgaag ttttttaggc accttttgaa atgtaatcat
     1441 ttgggtcaat atgtaatttt cagtgttaga ctagtaaatt gtccgctaaa ttctggccgt
     1501 ttttggcttt tttgttagac ggccaacttt tgtatacaaa gtgggccacc atgggaatcc
     1561 cgctgctgaa cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc
     1621 ctgtggtcga ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg
     1681 atgcttgcat tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct
     1741 ctccttttgg agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag
     1801 caatcggcct gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag
     1861 cagacaaggt gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag
     1921 gcatcctggt ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg
     1981 ggattaacgt ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac
     2041 tgcaggaagc agggattaac ctggacagga atactctggc cgctatgctg atccgagagc
     2101 tgcgggcagc cctggaactg ttcgagcagg aaggcctggc tccatatctg tcacggtggg
     2161 agaagctgga taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt
     2221 tcgggattag ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta
     2281 tcaaaccctg gatgggcgga gaaatcagtc tgcggtctgc cgaaaaggct agcggcaagc
     2341 ccatccccaa ccccctgctg ggcctggaca gcaccacaac tttgtacaaa aaagcaggct
     2401 ccaccatggc cagaaaggct ctcaagcttg cttcgtggac cagcatggct cttgctgcct
     2461 ctggcatcta cttctacagt aacaagtact tggaccctaa tgactttggc gctgtcaggg
     2521 tgggcagagc agttgctacg acggctgtca tcagttacga ctacctcact tccctgaaga
     2581 gtgtccctta tggctcagag gagtacttgc agctgagatc taaggtgcac cttcgctctg
     2641 ccaggcgtct ctgtgagctc tgctgtgcca accggggcac cttcatcaag gtgggccagc
     2701 acctgggggc tctggactac ctgttgccag aggagtacac cagcacgctg aaggtactgc
     2761 acagccaggc tccacagagc agcatgcaag agatccgcca ggtcatccga gaagatctgg
     2821 gcaaggagat ccatgatttg ttccagagct tcgatgacac ccctctgggg acggcctccc
     2881 tggcccaggt ccacaaggca gtgctgcatg atgggcggac ggtggccgtg aaggtccagc
     2941 acccaaaggt gcgggctcag agctcgaagg acattctcct gatggaggtg ctcgttctgg
     3001 ctgtgaagca gctgttccca gagtttgagt ttatgtggct tgtggatgaa gccaagaaga
     3061 acctgccttt ggagctggat ttcctcaatg aagggaggaa tgctgagaag gtgtcccaga
     3121 tgctcaggca ttttgacttc ttgaaggtcc cccgaatcca ctgggacctg tccacggagc
     3181 gggtcctcct gatggagttt gtggatggcg ggcaggtcaa tgacagagac tacatggaga
     3241 ggaacaagat cgacgtcaat gagatctcac gccacctggg caagatgtat agtgagatga
     3301 tcttcgtcaa tggcttcgtg cactgcgatc cccaccccgg caatgtactg gtgcggaagc
     3361 accccggcac gggaaaggcg gagattgtcc tgttggacca tgggctttac cagatgctca
     3421 cggaagaatt ccgcctgaat tactgccacc tctggcagtc tctgatctgg actgacatga
     3481 agagagtgaa ggagtacagc cagcgactgg gagccgggga tctctacccc ttgtttgcct
     3541 gcatgctgac ggcgcgatcg tgggactcgg tcaacagagg catcagccaa gctcccgtca
     3601 ctgccactga ggacttagag attcgcaaca acgcggccaa ctacctcccc cagatcagcc
     3661 atctcctcaa ccacgtgccg cgccagatgc tgctcatctt gaagaccaac gacctgctgc
     3721 gtggcattga ggccgccctg ggcacccgcg ccagcgccag ctcctttctc aacatgtcac
     3781 gttgctgcat cagagcgcta gctgagcaca agaagaagaa tacctgttca ttcttcagaa
     3841 ggacccagat ctctttcagc gaggccttca acttatggca gatcaacctc catgagctca
     3901 tcctgcgtgt gaaggggttg aagctggctg accgggtctt ggccctaata tgctggctgt
     3961 tccctgctcc actttaggac ccagctttct tgtacaaagt tgtgatatcc agcacagtgg
     4021 cggccgctcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     4081 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     4141 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     4201 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     4261 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     4321 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     4381 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     4441 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     4501 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     4561 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     4621 cctggaattc taccgggtag gggaggcgct tttcccaagg cagtctggag catgcgcttt
     4681 agcagccccg ctgggcactt ggcgctacac aagtggcctc tggcctcgca cacattccac
     4741 atccaccggt aggcgccaac cggctccgtt ctttggtggc cccttcgcgc caccttctac
     4801 tcctccccta gtcaggaagt tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca
     4861 aatggaagta gcacgtctca ctagtctcgt gcagatggac agcaccgctg agcaatggaa
     4921 gcgggtaggc ctttggggca gcggccaata gcagctttgc tccttcgctt tctgggctca
     4981 gaggctggga aggggtgggt ccgggggcgg gctcaggggc gggctcaggg gcggggcggg
     5041 cgcccgaagg tcctccggag gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc
     5101 gctgttctcc tcttcctcat ctccgggcct ttcgacctgc agcccaagct taccatgacc
     5161 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     5221 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     5281 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     5341 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     5401 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     5461 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     5521 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     5581 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     5641 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     5701 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     5761 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc tcgagggccc
     5821 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
     5881 aaagggggga ctggaagggc tagctcactc ccaacgaaga caagatctgc tttttgcttg
     5941 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     6001 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     6061 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     6121 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     6181 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     6241 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     6301 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     6361 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     6421 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     6481 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     6541 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     6601 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     6661 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     6721 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     6781 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     6841 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     6901 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     6961 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     7021 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     7081 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     7141 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     7201 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     7261 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     7321 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     7381 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     7441 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     7501 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     7561 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     7621 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     7681 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     7741 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     7801 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     7861 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     7921 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     7981 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     8041 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     8101 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     8161 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     8221 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     8281 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     8341 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     8401 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     8461 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     8521 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     8581 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     8641 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     8701 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     8761 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     8821 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     8881 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     8941 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     9001 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     9061 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctt tccccgcgcg
     9121 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     9181 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     9241 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     9301 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     9361 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     9421 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     9481 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     9541 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     9601 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     9661 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     9721 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     9781 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     9841 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     9901 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     9961 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
    10021 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
    10081 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
    10141 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
    10201 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
    10261 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
    10321 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
    10381 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
    10441 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
    10501 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
    10561 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
    10621 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
    10681 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
    10741 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
    10801 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
    10861 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
    10921 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
    10981 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggata
//
