LOCUS       sequence-310269-       10508 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway destination clone of CDK7 (human) tagged with N-terminal 
            miniTurbo-V5 for generating protein-proximity networks using 
            proximity-dependent biotinylation proteomics.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10508)
  TITLE     Major lab plasmids for proteomics-based protein-interaction and 
            protein-proximity network generation
REFERENCE   2  (bases 1 to 10508)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: p663-UBC-miniTurbo-V5-CDK7_IDG-K
FEATURES             Location/Qualifiers
     source          1..10508
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    182..298
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    332..352
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        352..1563
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1400..1419
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     protein_bind    complement(1567..1587)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     regulatory      1588..1597
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1600..2370
                     /codon_start=1
                     /product="N-terminally truncated mutant of the E. coli 
                     biotin ligase BioID engineered for improved catalytic 
                     efficiency (Branon et al., 2018)"
                     /label=miniTurbo
                     /translation="IPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGDACI
                     AEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLGADK
                     VRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWITLQ
                     EAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPVKLIIGDKEIF
                     GISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     CDS             2377..2418
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    2419..2443
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3494..3518)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3548..4136
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3601..3621)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4019..4030)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4150..4649
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4185..4208)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4582..4601
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4670..5269
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4670..4689)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5166..5186
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5407..5640
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5712..5833
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5749..5768)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5803..5822
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5873..6008
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6028..6047)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6029..6047)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6057..6074)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6057..6073)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6066..6088)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6215..6670
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6302..6321)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6512..6533
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6696..6800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6801..6869,6870..7661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7832..8420
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8321..8340
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8574..8591
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8708..8729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8744..8749,8750..8767,8768..8774)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8782..8798
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8787..8809
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8806..8822
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8806..8822
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8841..8861
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8843..8861
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8889..9115
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             9116..9296
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9343..9468
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9961..10194
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10379..10423
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
       61 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
      121 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcggttaa
      181 cttttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
      241 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaattc aaaattttat
      301 cgtccagtgt ggtggaattc tgcagatatc acaactttgt atagaaaagt tggcctccgc
      361 gccgggtttt ggcgcctccc gcgggcgccc ccctcctcac ggcgagcgct gccacgtcag
      421 acgaagggcg cagcgagcgt cctgatcctt ccgcccggac gctcaggaca gcggcccgct
      481 gctcataaga ctcggcctta gaaccccagt atcagcagaa ggacatttta ggacgggact
      541 tgggtgactc tagggcactg gttttctttc cagagagcgg aacaggcgag gaaaagtagt
      601 cccttctcgg cgattctgcg gagggatctc cgtggggcgg tgaacgccga tgattatata
      661 aggacgtgcc gggtgtggca cagctagttc cgtcgcagcc gggatttggg tcgcggttct
      721 tgtttgtgga tcgctgtgat cgtcacttgg tgagtagcgg gctgctgggc tggccggggc
      781 tttcgtggcc gccgggccgc tcggtgggac ggaagcgtgt ggagagaccg ccaagggctg
      841 tagtctgggt ccgcgagcaa ggttgccctg aactgggggt tggggggagc gcagcaaaat
      901 ggcggctgtt cccgagtctt gaatggaaga cgcttgtgag gcgggctgtg aggtcgttga
      961 aacaaggtgg ggggcatggt gggcggcaag aacccaaggt cttgaggcct tcgctaatgc
     1021 gggaaagctc ttattcgggt gagatgggct ggggcaccat ctggggaccc tgacgtgaag
     1081 tttgtcactg actggagaac tcggtttgtc gtctgttgcg ggggcggcag ttatggcggt
     1141 gccgttgggc agtgcacccg tacctttggg agcgcgcgcc ctcgtcgtgt cgtgacgtca
     1201 cccgttctgt tggcttataa tgcagggtgg ggccacctgc cggtaggtgt gcggtaggct
     1261 tttctccgtc gcaggacgca gggttcgggc ctagggtagg ctctcctgaa tcgacaggcg
     1321 ccggacctct ggtgagggga gggataagtg aggcgtcagt ttctttggtc ggttttatgt
     1381 acctatcttc ttaagtagct gaagctccgg ttttgaacta tgcgctcggg gttggcgagt
     1441 gtgttttgtg aagtttttta ggcacctttt gaaatgtaat catttgggtc aatatgtaat
     1501 tttcagtgtt agactagtaa attgtccgct aaattctggc cgtttttggc ttttttgtta
     1561 gacggccaac ttttgtatac aaagtgggcc accatgggaa tcccgctgct gaacgctaaa
     1621 cagattctgg gacagctgga cggcgggagc gtggcagtcc tgcctgtggt cgactccacc
     1681 aatcagtacc tgctggatcg aatcggcgag ctgaagagtg gggatgcttg cattgcagaa
     1741 tatcagcagg cagggagagg aagcagaggg aggaaatggt tctctccttt tggagctaac
     1801 ctgtacctga gtatgttttg gcgcctgaag cggggaccag cagcaatcgg cctgggcccg
     1861 gtcatcggaa ttgtcatggc agaagcgctg cgaaagctgg gagcagacaa ggtgcgagtc
     1921 aaatggccca atgacctgta tctgcaggat agaaagctgg caggcatcct ggtggagctg
     1981 gccggaataa caggcgatgc tgcacagatc gtcattggcg ccgggattaa cgtggctatg
     2041 aggcgcgtgg aggaaagcgt ggtcaatcag ggctggatca cactgcagga agcagggatt
     2101 aacctggaca ggaatactct ggccgctatg ctgatccgag agctgcgggc agccctggaa
     2161 ctgttcgagc aggaaggcct ggctccatat ctgtcacggt gggagaagct ggataacttc
     2221 atcaatagac ccgtgaagct gatcattggg gacaaagaga ttttcgggat tagccggggg
     2281 attgataaac agggagccct gctgctggaa caggacggag ttatcaaacc ctggatgggc
     2341 ggagaaatca gtctgcggtc tgccgaaaag gctagcggca agcccatccc caaccccctg
     2401 ctgggcctgg acagcaccac aagtttgtac aaaaaagcag gctccaccat ggctctggac
     2461 gtgaagtctc gggcaaagcg ttatgagaag ctggacttcc ttggggaggg acagtttgcc
     2521 accgtttaca aggccagaga taagaacacc aaccaaattg tcgccattaa gaaaatcaaa
     2581 cttggacata gatcagaagc taaagatggt ataaatagaa ccgccttaag agagataaaa
     2641 ttattacagg agctaagtca tccaaatata attggtctcc ttgatgcttt tggacataaa
     2701 tctaatatta gccttgtctt tgattttatg gaaactgatc tagaggttat aataaaggat
     2761 aatagtcttg tgctgacacc atcacacatc aaagcctaca tgttgatgac tcttcaagga
     2821 ttagaatatt tacatcaaca ttggatccta catagggatc tgaaaccaaa caacttgttg
     2881 ctagatgaaa atggagttct aaaactggca gattttggcc tggccaaatc ttttgggagc
     2941 cccaatagag cttatacaca tcaggttgta accaggtggt atcgggcccc cgagttacta
     3001 tttggagcta ggatgtatgg tgtaggtgtg gacatgtggg ctgttggctg tatattagca
     3061 gagttacttc taagggttcc ttttttgcca ggagattcag accttgatca gctaacaaga
     3121 atatttgaaa ctttgggcac accaactgag gaacagtggc cggacatgtg tagtcttcca
     3181 gattatgtga catttaagag tttccctgga atacctttgc atcacatctt cagtgcagca
     3241 ggagacgact tactagatct catacaaggc ttattcttat ttaatccatg tgctcgaatt
     3301 acggccacac aggcactgaa aatgaagtat ttcagtaatc ggccagggcc aacacctgga
     3361 tgtcagctgc caagaccaaa ctgtccagtg gaaaccttaa aggagcaatc aaatccagct
     3421 ttggcaataa aaaggaaaag aacagaggcc ttagaacaag gaggattgcc caagaaacta
     3481 attttttgaa ttcacccagc tttcttgtac aaagtggtga tatccagcac agtggcggcc
     3541 gctcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     3601 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     3661 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     3721 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3781 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3841 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3901 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     3961 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     4021 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     4081 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     4141 aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc gctttagcag
     4201 ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat tccacatcca
     4261 ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct tctactcctc
     4321 ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg tgacaaatgg
     4381 aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa tggaagcggg
     4441 taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg gctcagaggc
     4501 tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg gcgggcgccc
     4561 gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct gccgcgctgt
     4621 tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca tgaccgagta
     4681 caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc
     4741 cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga
     4801 gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt
     4861 gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga gcgtcgaagc
     4921 gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc
     4981 cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt
     5041 cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt
     5101 gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc
     5161 gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt
     5221 gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac gcccgcccca
     5281 cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatctcgag ggcccggtac
     5341 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5401 ggggactgga agggctagct cactcccaac gaagacaaga tctgcttttt gcttgtactg
     5461 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5521 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     5581 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     5641 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     5701 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     5761 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     5821 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     5881 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     5941 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6001 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6061 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6121 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6181 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6241 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6301 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6361 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6421 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6481 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6541 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     6601 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     6661 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     6721 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     6781 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     6841 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     6901 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6961 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7021 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7081 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7141 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7201 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7261 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7321 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7381 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7441 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7501 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7561 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     7621 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     7681 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     7741 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     7801 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     7861 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     7921 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7981 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8041 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8101 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8161 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8221 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8281 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8341 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     8401 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     8461 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     8521 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     8581 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     8641 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     8701 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     8761 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     8821 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     8881 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     8941 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     9001 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     9061 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     9121 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     9181 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     9241 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     9301 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     9361 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     9421 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     9481 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     9541 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     9601 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     9661 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     9721 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     9781 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     9841 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     9901 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9961 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
    10021 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
    10081 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
    10141 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
    10201 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
    10261 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
    10321 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
    10381 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
    10441 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
    10501 aagaatag
//
