LOCUS       sequence-310275-       13637 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG 
            (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13637)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 13637)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smFLAG_KDM5B_MS2
FEATURES             Location/Qualifiers
     source          1..13637
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2058..2175
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2218..2616
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      2622..2631
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2631..2654
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2658..2681
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2685..2708
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3213..3236
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3243..3266
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3279..3302
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3309..3332
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3525..3548
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3552..3575
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3579..3602
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             3978..3989
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    10027..10615
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10080..10100)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10498..10509)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10690..10923
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11042..11060)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11128..11232
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11233..11301,11302..12093)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11451..11470)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      12264..12852
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12753..12772
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     13006..13023
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    13263..13284
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(13299..13304,13305..13322,13323..13329)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    13337..13353
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     13342..13364
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     13361..13377
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13361..13377
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     13427..13446
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtggc
     2221 ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct cctcacggcg agcgctgcca
     2281 cgtcagacga agggcgcagc gagcgtcctg atccttccgc ccggacgctc aggacagcgg
     2341 cccgctgctc ataagactcg gccttagaac cccagtatca gcagaaggac attttaggac
     2401 gggacttggg tgactctagg gcactggttt tctttccaga gagcggaaca ggcgaggaaa
     2461 agtagtccct tctcggcgat tctgcggagg gatctccgtg gggcggtgaa cgccgatgat
     2521 tatataagga cgcgccgggt gtggcacagc tagttccgtc gcagccggga tttgggtcgc
     2581 ggttcttgtt tgtggatcgc tgtgatcgtc acttggcggc cgccaccatg gactacaagg
     2641 acgacgacga caaaggtgac tacaaagatg atgacgataa aggcgactat aaggacgatg
     2701 acgacaaggg cggaaactca ctgatcaagg aaaacatgcg gatgaaggtg gtgatggagg
     2761 gctccgtgaa tggtcaccag ttcaagtgca ccggagaggg agagggaaac ccgtacatgg
     2821 gaactcagac catgcgcatt aaggtcatcg aaggaggtcc gctgccgttc gctttcgata
     2881 tcctggccac ttcgttcgga ggagggtcgc gcacgttcat caagtacccg aagggaatcc
     2941 cggacttctt taagcagtca ttcccggaag gattcacttg ggaacgggtg acccggtatg
     3001 aagatggagg tgtggtgact gtcatgcaag atacttcgct ggaggatggg tgcctcgtgt
     3061 accacgtcca agtccgcgga gtgaatttcc cgtccaacgg accagtgatg cagaaaaaga
     3121 cgaagggttg ggaacctaat actgaaatga tgtaccccgc agacggaggg ctgaggggct
     3181 acacccacat ggcgctgaag gtcgacggag gagattacaa ggatgacgac gataagcaac
     3241 aagattacaa agacgatgat gacaagggcc agcagggcga ctacaaggac gacgacgaca
     3301 agcagcagga ctacaaagat gacgatgata aaggaggagg acatctgtcc tgttcgttcg
     3361 tgaccaccta cagatcaaag aaaaccgtgg gaaacatcaa gatgccgggc attcatgccg
     3421 tcgaccaccg cctggagcgg ctcgaagaat cagacaatga gatgttcgtc gtgcaaagag
     3481 aacatgccgt ggccaagttc gcgggactgg gaggcggtgg aggcgattac aaagacgatg
     3541 atgacaaggg tgactataaa gacgacgatg acaaagggga ttacaaggat gatgatgata
     3601 agggaggcgg tggatcaggt ggaggaggtt cactgcaggc gatcgccatg gaggcggcca
     3661 ccacactgca cccaggcccg cgcccggcgc tgcccctcgg gggcccgggc ccgctgggcg
     3721 agttcctgcc tccacccgag tgcccggtct tcgaacccag ctgggaagag ttcgcggacc
     3781 ccttcgcttt catccacaag atccggccca tagccgagca gactggcatc tgtaaggtgc
     3841 ggccgccgcc ggattggcag ccaccatttg catgtgatgt tgataaactt cattttacgc
     3901 cacgtatcca gagactgaat gaattggagg cccaaactcg tgtaaaattg aatttcttgg
     3961 accagattgc aaagtactgg gagttacagg gaagtactct gaaaattcca catgtggaga
     4021 ggaagatctt ggacttattt cagcttaata agttagttgc agaagaaggt ggatttgcag
     4081 ttgtttgcaa ggatagaaaa tggaccaaaa ttgctaccaa gatggggttt gctcctggca
     4141 aagcagtggg ctcacatatc agagggcatt atgaacgaat tctcaacccc tacaacttat
     4201 tcctgtccgg agacagccta aggtgtttgc agaagccaaa cctgaccaca gacactaagg
     4261 acaaggagta caaaccccat gatattcccc agaggcagtc tgtgcagcct tcggaaacgt
     4321 gccccccagc ccgacgagca aaacgcatga gagcagaggc catgaatatt aaaatagaac
     4381 ccgaggagac aacagaagcc agaactcata atctgagacg tcgaatgggt tgtccaactc
     4441 caaaatgtga aaatgagaaa gaaatgaaga gtagcatcaa gcaagaacct attgagagga
     4501 aagattatat tgtagaaaat gagaaggaaa agcccaagag tcgatctaaa aaagccacca
     4561 atgctgtgga cctgtatgtc tgtcttttat gtggcagtgg caatgatgaa gaccggctac
     4621 tgttgtgtga tggctgtgat gacagttacc ataccttttg cttgatccca cctctccatg
     4681 atgttcccaa gggagactgg aggtgtccta agtgtttggc tcaggaatgt agtaagccac
     4741 aagaagcatt tggctttgaa caagcagcca gggactatac cctccgtact tttggggaaa
     4801 tggcagatgc gttcaaatct gattacttca acatgccagt ccatatggtc cccacagagc
     4861 ttgttgagaa agaattttgg agactagtaa gcactattga ggaggatgtc acagtggaat
     4921 atggagctga cattgcctca aaggaatttg gcagtggctt tcctgtccga gatgggaaaa
     4981 tcaaactctc acctgaggaa gaggagtatc ttgatagtgg ctggaatttg aacaacatgc
     5041 cagtgatgga gcagtctgtc cttgcacata ttactgctga tatatgtggc atgaaacttc
     5101 cttggttgta tgtgggaatg tgcttttctt cattctgttg gcacattgaa gaccactgga
     5161 gctattcaat taactacttg cactggggtg agccaaaaac ctggtatgga gtcccagggt
     5221 atgctgctga gcagctagaa aatgtaatga agaaactagc tccagaactc tttgtgtccc
     5281 agccggatct cctccatcag cttgtgacca tcatgaaccc caataccctg atgactcatg
     5341 aagtgcctgt ttaccgaact aatcagtgtg ctggggagtt tgtgattaca tttccaagag
     5401 cctaccacag tggttttaac cagggtttta attttgctga ggctgttaac ttctgcactg
     5461 ttgattggct gccattaggc cgacagtgtg tggagcatta tcgcttgctt catcgatatt
     5521 gtgtgttttc ccacgatgag atgatctgca agatggcttc caaggctgat gtattagatg
     5581 ttgtagtggc ttcaactgtt cagaaagaca tggccattat gattgaggat gagaaagctt
     5641 taagagaaac tgtccgtaaa ttgggagtga ttgattcgga aagaatggat tttgagctgt
     5701 tgccagatga tgaacgtcag tgtgtaaaat gcaaaactac atgcttcatg tctgccatct
     5761 cctgttcttg taaacctggc cttcttgttt gcctgcatca tgtaaaagaa ttgtgttcct
     5821 gtcctcctta taaatataaa ttgcggtata ggtacacgct ggatgatctc taccctatga
     5881 tgaatgcatt gaagcttcga gcagaatctt acaacgaatg ggccttgaat gtgaatgaag
     5941 ctttggaggc aaagatcaac aagaagaaaa gccttgtcag cttcaaggct ttaattgaag
     6001 aatctgaaat gaagaaattc ccagacaatg atcttttgcg acaccttcgc ctagtcacac
     6061 aggatgcaga gaagtgtgcc tctgttgcgc agcagttgct taatggcaaa aggcaaacta
     6121 gatatcgatc tggtggaggg aaatcccaaa atcagttgac agtgaatgag ctccggcagt
     6181 ttgtaacaca gctgtatgct cttccatgtg tcctcagtca gacaccatta ctaaaggatc
     6241 tcttgaatcg tgtagaagat tttcaacagc atagtcagaa actactctct gaggaaacgc
     6301 ctagtgctgc ggagctgcag gacttgctag atgtcagctt tgaatttgat gttgaacttc
     6361 cacagcttgc tgagatgcgt atccgtttgg aacaagcccg ttggctagaa gaggtgcagc
     6421 aagcttgcct agaccccagc tcccttactt tagatgatat gagacgtctc atagacctag
     6481 gggtagggct ggccccgtat tcagcagtgg agaaagctat ggcccggctg caggaactgc
     6541 tcacagtgtc agagcactgg gacgacaaag ccaagagtct cctcaaggcc aggccacgac
     6601 attcattgaa tagccttgct acggcagtaa aggaaatcga agagatccct gcatatctgc
     6661 ccaatggtgc ggctctgaaa gactcagtgc agagagccag agactggctt caggatgtag
     6721 agggcctgca ggctggagga cgtgtgccag tgttagacac actcatagaa cttgttacac
     6781 gaggccgatc tatcccggta catctgaatt ctttgccaag actggaaacc ctagtagctg
     6841 aggttcaggc ttggaaagaa tgtgctgtta atacattctt gactgagaat tctccatatt
     6901 ctctcttaga ggtgctgtgt cctcgatgtg atattggcct tttgggattg aaaaggaagc
     6961 agagaaagtt aaaggagccc ttgccaaatg gaaagaaaaa aagcaccaaa ttagagagtc
     7021 tgagtgacct ggagagagct ttaactgaaa gcaaggagac tgcttcagct atggcaactc
     7081 ttggggaagc tcgcctaagg gaaatggaag ccttgcagtc tctcagactc gccaatgaag
     7141 ggaaattgct gtcgcctctc caagatgtgg atataaaaat ctgcctatgt cagaaggccc
     7201 cagctgcccc tatgattcaa tgtgaactct gcagggatgc tttccacacc agttgtgtgg
     7261 cggtacccag tatttcacag ggcctgcgaa tctggctttg tccccattgt cggaggtcag
     7321 agaaacctcc attagagaaa attctgcccc tgctcgcctc ccttcagcgt atccgagttc
     7381 gccttcctga gggagatgca cttcgatata tgattgaaag aaccgtgaac tggcagcaca
     7441 gagcccagca actgctttcg tcagggaatc ttaaatttgt gcaagatcga gtgggctcag
     7501 gactgttata tagcagatgg caagcctcag caggacaggt gtcagacaca aacaaggtat
     7561 ctcaacctcc tggcacaaca tcattttctt tgcctgatga ctgggacaac agaacctcat
     7621 atttgcactc ccccttctca actggacgaa gttgtatccc cctccatggt gttagtccag
     7681 aagtgaatga actattgatg gaagcccagc tgctccaggt atcccttcct gaaattcagg
     7741 aactttacca gactttactt gcaaagccaa gccctgctca gcagactgac cgaagctcac
     7801 cagtgagacc cagcagtgag aagaatgact gttgccgagg gaagcgagat ggaattaaca
     7861 gtcttgagag aaaactgaag agacgcctgg aaagagaggg cctctccagt gagcggtggg
     7921 aacgagttaa gaaaatgcgg acccccaaaa agaagaaaat caaactgagc caccccaagg
     7981 acatgaacaa tttcaagtta gagagagagc gtagctatga attagttcgt tctgctgaaa
     8041 ctcattccct gccctcagac acatcctatt ccgaacagga agactctgag gatgaagatg
     8101 ccatctgccc agctgtgagc tgcctgcagc cagaaggaga tgaggtggac tgggtccagt
     8161 gtgatggcag ctgcaatcag tggtttcatc aggtctgtgt tggtgtctcc ccagagatgg
     8221 cagagaaaga agactacatc tgtgtgcgct gtactgtgaa ggacgcacca agccgaaagg
     8281 tttaaacgct agctaggcgg actgttactg agctgcgttt tacacccttt ctttgacaaa
     8341 acctaacttg cgcagaaaaa aaaaaaataa gagacaacat tggcatggct ttgttttttt
     8401 aaattttttt taaagttttt tttttttttt tttttttttt aagttttttt gttttgtttt
     8461 ggcgcttttg actcaggatt taaaaactgg aacggtgaag gcgacagcag ttggttggag
     8521 caaacatccc ccaaagttct acaaatgtgg ctgaggactt tgtacattgt tttgtttttt
     8581 ttttttttgg ttttgtcttt ttttaatagt cattccaagt atccatgaaa taagtggtta
     8641 caggaagtcc ctcaccctcc caaaagccac ccccactcct aagaggagga tggtcgcgtc
     8701 catgccctga gtccaccccg gggaaggtga caaccggtaa cctacaaacg ggtggaggat
     8761 caccccaccc gacacttcac aatcaagggg tacaatacac aagggtggag gaacacccca
     8821 ccctccagac acattacaca gaaatccaat caaacagaag caccatcagg gcttctgcta
     8881 ccaaatttat ctcaaaaaac tacaacaagg aatcaccatc agggattccc tgtgcaatat
     8941 acgtcaaacg agggccacga cgggaggacg atcacgcctc ccgaatatcg gcatgtctgg
     9001 ctttcgaatt cagtgcgtgg agcatcagcc cacgcagcca atcagagtcg aatacaagtc
     9061 gactttcgcg aagagcatca gccttcgcgc cattcttaca caaaccacac tctcccctac
     9121 aggaacagca tcagcgttcc tgcccagtac ccaactcaag aaaatttatg tccccatgca
     9181 gcatcagcgc atgggcccca agaatacatc cccaacaaaa tcacatccga gcaccaacag
     9241 ggctcggagt gttgtttctt gtccaactgg acaaaccctc catggaccat caggccatgg
     9301 actctcacca acaagacaaa aactactctt ctcgaagcag catcagcgct tcgaaacact
     9361 cgagcataca ttgtgcctat ttcttgggtg gacgatcacg ccacccatgc tctcacgaat
     9421 ttcaaaacac ggacaaggac gagcaccacc agggctcgtc gttccacgtc caatacgatt
     9481 acttaccttt cgggatcacg atcacggatc ccgcagctac atcacttcca ctcaggacat
     9541 tcaagcatgc acgatcacgg catgctccac aagtctcaac cacagaaact accaaatggg
     9601 ttcagcacca gcgaacccac tcctacctca aacctcttcc cacaaaactg gcaagcagga
     9661 tcaccgcttg cccattccaa cataccaaat caaaaacaat tactggtaca gcatcagcgt
     9721 accagcccac atctctcact actatcaaaa accaaaccgt tcagcaacag cgaacggtac
     9781 acacggaaaa atcaactggt ttacaaatac gaaagacgat cacgctttcg tccagcgcaa
     9841 actattacga aaaacatccg acgggaagag caacagcctt cccgcggcgg aaaacctcac
     9901 aaaaacacga caaacggatg cacgaacacg gcatccgccg acaacccaca aacttacaac
     9961 caggcaaacg gtgcaggatc accgcaccgt acatcaaaca cctcagatct catatcgata
    10021 gatcctaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
    10081 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
    10141 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
    10201 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
    10261 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
    10321 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
    10381 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg
    10441 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
    10501 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
    10561 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgag
    10621 atcctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
    10681 aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt tttgcttgta
    10741 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
    10801 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
    10861 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
    10921 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
    10981 cagagagtga gaggcccggg ttaattaagg aaagggctag atcattcttg aagacgaaag
    11041 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
    11101 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
    11161 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
    11221 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
    11281 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
    11341 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
    11401 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
    11461 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
    11521 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
    11581 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
    11641 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
    11701 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
    11761 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
    11821 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
    11881 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
    11941 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
    12001 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
    12061 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
    12121 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
    12181 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
    12241 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
    12301 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
    12361 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
    12421 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
    12481 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
    12541 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
    12601 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
    12661 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
    12721 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
    12781 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
    12841 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
    12901 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
    12961 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
    13021 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
    13081 taatgcagca agctcatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
    13141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca
    13201 aaaagctccc cgtggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca
    13261 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    13321 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgacatg
    13381 attacgaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    13441 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    13501 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    13561 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    13621 actacaaact tagtagt
//
