LOCUS       sequence-310293-         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlightN-CymR-VP16-3X-SV40-attB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS
  TITLE     Spatial control of gene expression in flies using bacterially 
            derived binary transactivation systems.
  JOURNAL   Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. 
            Epub 2021 May 24.
  PUBMED    33963794
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/imb.12717";
            journalName: "Insect Mol Biol"; date: "2021-10"; volume: "30"; 
            issue: "5"; pages: "461-471"
COMMENT     Sequence Label: 907H1
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..609
                     /codon_start=1
                     /gene="cymR from Pseudomonas putida"
                     /product="cumate repressor (Mullick et al., 2006)"
                     /label=CymR
                     /translation="MSPKRRTQAERAMETQGKLIAAALGVLREKGYAGFRIADVPGAAG
                     VSRGAQSHHFPTKLELLLATFEWLYEQITERSRARLAKLKPEDDVIQQMLDDAAEFFLD
                     DDFSISLDLIVAADRDPALREGIQRTVERNRFVVEDMWLGVLVSRGLSRDDAEDILWLI
                     FNSVRGLAVRSLWQKDKERFERVRNSTLEIARERYAKFKR"
ORIGIN
        1 atgagtccaa agagaagaac acaggcagag cgcgcaatgg agacccaggg caagttgatt
       61 gcagcggccc tgggggtttt acgggaaaaa ggttacgcgg gattccggat cgcagatgtg
      121 cccggtgctg caggtgtctc gagaggagcg cagagccatc atttcccgac aaagcttgag
      181 cttctgcttg ccacttttga atggctttac gaacagatca ccgaacgcag tcgggctcga
      241 ttagcgaaat tgaagccaga ggatgacgtc atccagcaaa tgctggacga cgccgccgaa
      301 tttttcctcg acgatgactt ctctatcagc cttgatttga ttgtggctgc cgaccgggat
      361 ccagcgttac gcgagggtat tcagcgcacg gtagagagga atcggtttgt cgtcgaggat
      421 atgtggcttg gtgttctggt gagccgtggt ctttcgcgtg atgatgcaga agatatcctt
      481 tggttgatat tcaattcggt gcgtgggctt gctgttcgta gcctatggca gaaggacaaa
      541 gaacgctttg agcgtgtcag gaactcgaca ctcgaaattg cgcgagagcg gtacgcgaaa
      601 ttcaagcgc
//
