LOCUS       sequence-310298-       14075 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PipO(3x)-Hsp70min-UASpmin-Firefly-T2A-eGFP-p10-3'UTR-Ubiquitin-Renil
            la-attB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14075)
  AUTHORS   Gamez S, Vesga LC, Mendez-Sanchez SC, Akbari OS
  TITLE     Spatial control of gene expression in flies using bacterially 
            derived binary transactivation systems.
  JOURNAL   Insect Mol Biol. 2021 Oct;30(5):461-471. doi: 10.1111/imb.12717. 
            Epub 2021 May 24.
  PUBMED    33963794
REFERENCE   2  (bases 1 to 14075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/imb.12717";
            journalName: "Insect Mol Biol"; date: "2021-10"; volume: "30"; 
            issue: "5"; pages: "461-471"
COMMENT     Sequence Label: 908G
FEATURES             Location/Qualifiers
     source          1..14075
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(91..110)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     301..322
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             5397..7046
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5463..5481)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     CDS             7047..7100
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(7101..7103,7104..7106,7107..7820)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTSKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7146..7167)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7407..7426)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7754..7775
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8624..8705
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8661..8680)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8715..8734
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     CDS             complement(8748..9683)
                     /codon_start=1
                     /product="luciferase from the anthozoan coelenterate 
                     Renilla reniformis (sea pansy)"
                     /label=Rluc
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     protein_bind    11599..11668
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(11887..11905)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11926..11942)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11926..11942)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11939..11961)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11950..11966
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11974..11980,11981..11998,11999..12004))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12019..12040
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12157..12174)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12328..12916)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12408..12427)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13087..13878,13879..13947))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13710..13729
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13948..14052)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atccgggaat
     4861 tgggaattgg gcaatattta aatggcggct cgaggcgcta ttttcattca ggctccttgt
     4921 acggtgtacg ggaagatact cgtacaccgt acaagagatt cccatacgct gtacagcgct
     4981 atttggatcc atgcggccct gcagagcgcc ggagtataaa tagaggcgct tcgtctacgg
     5041 agcgacaatt caattcaaac aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat
     5101 aaacaagcgc agctgaacaa gctaaacaat ctgccaggcc tccgtagctt accgaagtat
     5161 acacttaaat tcagtgcacg tttgcttgtt gagaggaaag gttgtgtgcg gacgaatttt
     5221 tttttgaaaa ccggtgatag agcctgaacc agaaaagata aaagaaggct ataccagtgg
     5281 gagtacacaa acagagtaag tttgaatagt aaaaaaaatc atttatgtaa acaataacgt
     5341 gactgtgcgt taggtcctgt tcattggtac ccgcccgggg atcagaattg cctaggatgg
     5401 aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa gatggaaccg
     5461 ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga acaattgctt
     5521 ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa atgtccgttc
     5581 ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca
     5641 gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc ggagttgcag
     5701 ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg ggcatttcgc
     5761 agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa
     5821 agctcccaat catccaaaaa attattatca tggattctaa aacggattac cagggatttc
     5881 agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa tacgattttg
     5941 tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc tctggatcta
     6001 ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga ttctcgcatg
     6061 ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta agtgttgttc
     6121 cattccatca cggttttgga atgtttacta cactcggata tttgatatgt ggatttcgag
     6181 tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag gattacaaga
     6241 ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc actctgattg
     6301 acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc ctctctaagg
     6361 aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa ggatatgggc
     6421 tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa ccgggcgcgg
     6481 tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc gggaaaacgc
     6541 tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg tccggttatg
     6601 taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta cattctggag
     6661 acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg aagtctctga
     6721 ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg ctccaacacc
     6781 ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt gaacttcccg
     6841 ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc gtggattacg
     6901 tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt gtggacgaag
     6961 taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc ctcataaagg
     7021 ccaagaaggg cggaaagatc gccgtggagg gcagaggaag tcttctaaca tgcggtgacg
     7081 tggaggagaa tcccggccct atggtgagca agggcgagga gctgttcacc ggggtggtgc
     7141 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
     7201 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accagcaagc
     7261 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
     7321 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
     7381 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
     7441 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
     7501 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
     7561 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
     7621 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
     7681 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
     7741 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
     7801 tggacgagct gtacaagtaa gcgatcgcct agaatgaatc gtttttaaaa taacaaatca
     7861 attgttttat aatattcgta cgattctttg attatgtaat aaaatgtgat cattaggaag
     7921 attacgaaaa atataaaaaa tatgagttct gtgtgtataa caaatgctgt aaacgccaca
     7981 attgtgtttg ttgcaaataa acccatgatt atttgattaa aattgttgtt ttctttgttc
     8041 atagacaata gtgtgttttg cctaaacgtg tactgcataa actccatgcg agtgtatagc
     8101 gagctagtgg ctaacgcttg ccccaccaaa gtagattcgt caaaatcctc aatttcatca
     8161 ccctcctcca agtttaacat ttggccgtcg gaattaactt ctaaagatgc cacataatct
     8221 aataaatgaa atagagattc aaacgtggcg tcatcgtccg tttcgaccat ttccgaaaag
     8281 aactcgggca taaactctat gatttctctg gacgtggtgt tgtcgaaact ctcaaagtac
     8341 gcagtcagga acgtgcgcga catgtcgtcg ggaaactcgc gcggaaacat gttgttgtaa
     8401 ccgaacgggt cccatagcgc caaaaccaaa tctgccagcg tcaatagaat gagcacgatg
     8461 ccgacaatgg agctggcttg gatagcgatt cgagttaacg gcccttaagg gtcgactgat
     8521 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     8581 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     8641 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     8701 actgcattct agttgtggtt tgtccaaact catcaatgtg gcgcgcctta ttgttcattt
     8761 ttgagaactc gctcaacgaa cgatttgata tattttccca tttcatcagg tgcatcttct
     8821 tgcgaaaaat gaagaccttt tactttgaca aattcagtat taggaaactt cttggcacct
     8881 tcaacaatag cattggaaaa gaatcctggg tccgattcaa taaacatttt tggtaaatca
     8941 tcacttgcac gtagataagc attataattc ctaacaattt gtacaacgtc aggtttacca
     9001 ccttttacta acgggatttc acgaggccat gataatgttg gacgacgaac ttcacctttc
     9061 tctttgaatg gttcaagata tgctgcaaat tcttctggtt ctaactttct catgattttt
     9121 gatggcaaca tggtttccac gaagaagtta ttctccaaaa ccattttttc tccttcttca
     9181 gatttgatca acgcaatatc ttcttcaata tcaggccatt catcccatga ttcaatcaca
     9241 tctactacac tttcagcgtg aactattgct ttgatcttat cttgatgctc atagctataa
     9301 tgaaatgcca aacaagcacc ccaatcatgg ccgacaaaaa tgatcttctt tggtaaatta
     9361 agaagttcaa accatgcagt aagatatttg taatgatcaa gtaacctata agaaccatta
     9421 ccagatttgc ctgatttgcc cataccaata aggtctggta taatacaccg cgctactggc
     9481 tcaatatgtg gcacaacatg tcgccataaa taagaagagg ccgcgttacc atgtaaaaaa
     9541 ataacagcat tttctgcatg tttttctgaa tcataataat taataaatga atcaagaaca
     9601 ttcatttgtt tacatctggc ccaccactgc ggaccagtta tcatccgttt cctttgttct
     9661 ggatcataaa ctttcgaagt catgcggccg ctctgcgggt caaaatagag atgtggaaaa
     9721 ttagtacgaa atcaaatgag tttcgttgaa attacaaaac tattgaaact aacttcctgg
     9781 ctggggaata aaaatgggaa acttatttat cgacgccaac tttgttgaga aacccctatt
     9841 aaccctctac gaatattgga acaaaggaaa gcgaagaaac aggaacaaag gtagttgaga
     9901 aacctgttcc gttgctcgtc atcgttttca taatgcgagt gtgtgcatgt atatatacac
     9961 agctgaaacg catgcataca cattattttg tgtgtatatg gtgacgtcac aactactaag
    10021 caataagaaa ttttccagac gtggctttcg tttcaagcaa cctactctat ttcagctaaa
    10081 aataagtgga tttcgttggt aaaatacttc aattaagcaa agaactaact aactaataac
    10141 atgcacacaa atgcaatatg tgcgttcgtg atttctcgaa ttttcaaatg cgtcactgcg
    10201 aatttcacaa tttgccaata aatcttggcg aaaatcaaca cgcaagtttt atttatagat
    10261 ttgtttgcgt tttgatgcca attgattggg aaaacaagat gcgtggctgc caatttctta
    10321 ttttgtaatt acgtagagcg ttgaataaaa aaaaaatggc cgaacaaaga ccttgaaatg
    10381 cagtttttct tgaaattact caacgtcttg ttgctcttat tactaattgg taacagcgag
    10441 ttaaaaactt acgtttcttg tgactttcga gaatgttctt ttaattgtac tttaatcacc
    10501 aacaattaag tataaatttt tcgctgattg cgctttactt tctgcttgta cttgctgctg
    10561 caaatgtcaa ttggttttga aggcgaccgt tcgcgaacgc tgtttatata ccttcggtgt
    10621 ccgttgaaaa tcactaaaaa ataccgtagt gttcgtaaca ctttagtaca gagaaaaaaa
    10681 attgtgccga aatgtttttg atacgtacga ataccttgta ttaaaatttt ttatgatttc
    10741 tgtgtatcac tttttttttg tgtttttcgt ttaaactcac cacagtacaa aacaataaaa
    10801 tatttttaag acaatttcaa attgagacct ttctcgtact gacttgaccg gctgaatgag
    10861 gatttctacc tagacgacct acttcttacc atgacattga atgcaatgcc acctttgatc
    10921 taaacttaca aaagtccaag gcttgttagg attggtgttt tggaatttaa gacccttgtt
    10981 agtttgcttt tgaaatagca ctgtcttctc taccggctat aattttgaaa ctcgcagctt
    11041 gactggaaat ttaaaaagta attctgtgta ggtaaagggt gttttaaaag tgtgatgtgt
    11101 tgagcgttgc ggcaacgact gctatttatg tatatatttt caaaacttat tgtttttgaa
    11161 gtgttttaaa tggagctatc tggcaacgct gcgcataatc ttacacaagc ttttcttaat
    11221 ccatttttaa gtgaaatttg tttatactct ttcggcaaat aattgttaaa tcgctttaag
    11281 tgggcttaca tctggataag taatgaaaac ctgcatatta taatattaaa acatataatc
    11341 cactgtgctt tccccgtgtg tggccatata cctaaaaaag tttattttcg cagagccccg
    11401 cacggtcaca ctacggttcg gcgattttcg attttggaca gtactgattg caagcgcacc
    11461 gaaagcaaaa tggagctgga gattttgaac gcgaagaaca gcaagccgta cggcaaggtg
    11521 aaggtgccct ccggcgccac gcccatcggc gatctgcgcg tctagagatc cactagtgtc
    11581 gacgatgtag gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc
    11641 cccgggcgcg tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg
    11701 gtagttgatc ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg
    11761 cgcggtggtc acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg
    11821 cagcgtcagc gggttctcga cggtcacggc gggcatgtcg acactagttc tagccagctt
    11881 ttgttccctt tagtgagggt taatttcgag cttggcgtaa tcatggtcat agctgtttcc
    11941 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
    12001 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
    12061 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
    12121 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
    12181 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
    12241 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
    12301 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
    12361 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
    12421 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
    12481 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
    12541 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
    12601 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
    12661 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
    12721 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
    12781 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
    12841 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
    12901 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
    12961 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
    13021 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
    13081 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
    13141 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
    13201 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
    13261 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
    13321 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
    13381 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
    13441 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
    13501 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
    13561 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
    13621 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
    13681 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
    13741 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
    13801 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
    13861 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
    13921 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
    13981 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
    14041 aggggttccg cgcacatttc cccgaaaagt gccac
//
