LOCUS       sequence-310312-        2880 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-term 8xHis 3C cleavable Amp resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 4 pCOLI_A_3c_C_HIS
FEATURES             Location/Qualifiers
     source          1..2880
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     324..343
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     577..594
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    609..748
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(674..693)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     834..856
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(850..1041)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     1278..1297
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1278..1296
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1334..1356
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1416..1439
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             1446..1469
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     primer_bind     complement(1563..1581)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1577..1624
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1689..1780
                     /gene="bla"
                     /label=AmpR promoter
     terminator      2645..2684
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     rep_origin      join(2715..2880,1..423)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
       61 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
      121 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
      181 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
      241 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
      301 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
      361 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
      421 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
      481 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
      541 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
      601 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
      661 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
      721 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
      781 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
      841 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
      901 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
      961 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
     1021 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatggggta
     1081 atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc
     1141 cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga
     1201 aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tggcaccatg
     1261 catcgatccc gcgaaattaa tacgactcac tatagggaga ccacaacggt ttccctccgg
     1321 ctccggaaat aattttgttt aactttaaga aggagactgg atccgaatct agagcctgca
     1381 gtctcgacaa gcttggaagc ggatctggat ctggactgga agttctgttc caggggcccg
     1441 gtagccatca tcatcatcat caccatcact aataagtcga gaagtactag aggatgtaca
     1501 acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc
     1561 caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt
     1621 tttgctgaaa ggaggaacta tatcgggatt agcccgccta atgagcgggc ttttttttaa
     1681 ttcccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     1741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     1801 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     1861 gctcgtgaaa gtaaaagacg cagaggacca attgggggca cgagtgggat acatagaact
     1921 ggacttgaat agcggtaaaa tccttgagag ttttcgccct gaagagcgtt ttccaatgat
     1981 gagcactttc aaagttctgc tatgtggagc agtattatcc cgtgtagatg cggggcaaga
     2041 gcaactcgga cgacgaatac actattcgca gaatgacttg gttgaatact ccccagtgac
     2101 agaaaagcac cttacggacg gaatgacggt aagagaatta tgtagtgccg ccataacgat
     2161 gagtgataac actgcggcga acttacttct gacaaccatc ggtggaccga aggaattaac
     2221 cgcttttttg cacaatatgg gagaccatgt aactcgcctt gaccgttggg aaccagaact
     2281 gaatgaagcc ataccaaacg acgagcgaga caccacaatg cctgcggcaa tggcaacaac
     2341 attacgcaaa ctattaactg gcgaactact tactctggct tcacggcaac aattaataga
     2401 ctggcttgaa gcggataaag ttgcaggacc actactgcgt tcggcacttc ctgctggctg
     2461 gtttattgct gataaatctg gggcaggaga gcgtggttca cggggtatca ttgccgcact
     2521 tggaccagat ggtaagcctt cccgtatcgt agttatctac acgacgggta gtcaggcaac
     2581 tatggacgaa cgaaatagac agattgctga aataggggct tcactgatta agcattggta
     2641 aaccgataca attaaaggct ccttttggag cctttttttt tggacggacc ggtagaaaag
     2701 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     2761 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     2821 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
//
