LOCUS       sequence-310324-        2455 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term 6xHis 3C cleavable Spec resistant bacterial expression 
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 26 pCOLI_S_HIS
FEATURES             Location/Qualifiers
     source          1..2455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             152..169
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             182..205
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(398..416)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      412..459
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(630..1421)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1422..1513)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1561..2299)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctaatacg
       61 actcactata gggagaccac aacggtttcc ctccggctcc ggaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgggcagcag ccatcatcat catcatcaca gcagcggatc
      181 tctggaagtt ctgttccagg ggcccggatc ccggtccgac atatgagcgc gcggaattca
      241 aaggcctacg tcgacgagct cactagtcgc ggccgctttc gaatctagag cctgcagtct
      301 cgacaagctt gtcgagaagt actagaggat gtacaacgcg tgcgcgctga tccggctgct
      361 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      421 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag
      481 aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa
      541 gcggctattt aacgaccctg ccctgaaccg acgaccgggt catcgtggcc ggatcttgcg
      601 gcccctcggc ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct
      661 ttcacgtagt ggacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct
      721 tgtccaagat aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc
      781 tccattgccc agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac
      841 caaatgcggg acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag
      901 ttccatagcg ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca
      961 aagagttcct ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc
     1021 aagatagcca gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg
     1081 cgctgccatt ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg
     1141 tcgtgcacaa caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc
     1201 gaagtttcca aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc
     1261 accgtaacca gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg
     1321 tacaaatgta cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct
     1381 gatagttgag tcgatacttc ggcgatcacc gcttccctca tactcttcct ttttcaatat
     1441 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     1501 aaaaataaac aaatagctag ctcactcggt cgctacgctc cgggcgtgag actgcggcgg
     1561 gcgctgcgga cacatacaaa gttacccaca gattccgtgg ataagcaggg gactaacatg
     1621 tgaggcaaaa cagcagggcc gcgccggtgg cgtttttcca taggctccgc cctcctgcca
     1681 gagttcacat aaacagacgc ttttccggtg catctgtggg agccgtgagg ctcaaccatg
     1741 aatctgacag tacgggcgaa acccgacagg acttaaagat ccccaccgtt tccggcgggt
     1801 cgctccctct tgcgctctcc tgttccgacc ctgccgttta ccggatacct gttccgcctt
     1861 tctcccttac gggaagtgtg gcgctttctc atagctcaca cactggtatc tcggctcggt
     1921 gtaggtcgtt cgctccaagc tgggctgtaa gcaagaactc cccgttcagc ccgactgctg
     1981 cgccttatcc ggtaactgtt cacttgagtc caacccggaa aagcacggta aaacgccact
     2041 ggcagcagcc attggtaact gggagttcgc agaggatttg tttagctaaa cacgcggttg
     2101 ctcttgaagt gtgcgccaaa gtccggctac actggaagga cagatttggt tgctgtgctc
     2161 tgcgaaagcc agttaccacg gttaagcagt tccccaactg acttaacctt cgatcaaacc
     2221 acctccccag gtggtttttt cgtttacagg gcaaaagatt acgcgcagaa aaaaaggatc
     2281 tcaagaagat cctttgatct tttctactga accgctctag atttcagtgc aatttatctc
     2341 ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct catgttagtc
     2401 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcg
//
