LOCUS       sequence-310329-        2560 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term HA 3C cleavable Spec resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 32 pCOLI_S_HA
FEATURES             Location/Qualifiers
     source          1..2560
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(128..219)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(267..1005)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1215..1234
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1215..1233
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1271..1293
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1313..1330
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1343..1369
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1376..1402
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1409..1435
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1448..1471
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1664..1682)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1678..1725
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(1896..2560,1..127))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tcaggccgcc atccactgcg gagccgtaca aatgtacggc cagcaacgtc ggttcgagat
       61 ggcgctcgat gacgccaact acctctgata gttgagtcga tacttcggcg atcaccgctt
      121 ccctcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      181 gcggatacat atttgaatgt atttagaaaa ataaacaaat agctagctca ctcggtcgct
      241 acgctccggg cgtgagactg cggcgggcgc tgcggacaca tacaaagtta cccacagatt
      301 ccgtggataa gcaggggact aacatgtgag gcaaaacagc agggccgcgc cggtggcgtt
      361 tttccatagg ctccgccctc ctgccagagt tcacataaac agacgctttt ccggtgcatc
      421 tgtgggagcc gtgaggctca accatgaatc tgacagtacg ggcgaaaccc gacaggactt
      481 aaagatcccc accgtttccg gcgggtcgct ccctcttgcg ctctcctgtt ccgaccctgc
      541 cgtttaccgg atacctgttc cgcctttctc ccttacggga agtgtggcgc tttctcatag
      601 ctcacacact ggtatctcgg ctcggtgtag gtcgttcgct ccaagctggg ctgtaagcaa
      661 gaactccccg ttcagcccga ctgctgcgcc ttatccggta actgttcact tgagtccaac
      721 ccggaaaagc acggtaaaac gccactggca gcagccattg gtaactggga gttcgcagag
      781 gatttgttta gctaaacacg cggttgctct tgaagtgtgc gccaaagtcc ggctacactg
      841 gaaggacaga tttggttgct gtgctctgcg aaagccagtt accacggtta agcagttccc
      901 caactgactt aaccttcgat caaaccacct ccccaggtgg ttttttcgtt tacagggcaa
      961 aagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tactgaaccg
     1021 ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag ccccatacga
     1081 tataagttgt aattctcatg ttagtcatgc cccgcgccca ccggaaggag ctgactgggt
     1141 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat
     1201 taattgcgtt gcgctaatac gactcactat agggagacca caacggtttc cctccggctc
     1261 cggaaataat tttgtttaac tttaagaagg agatatacat atggggagtt cgcaccatca
     1321 tcaccaccat agctctggct cctatccgta tgacgtgcca gactatgctg gtagctatcc
     1381 ttacgatgtc cccgattatg cgggatctta tccatacgat gtgccagact atgcaagttc
     1441 aggttccctg gaagttctgt tccaggggcc cggatcccgg tccgacatat gagcgcgcgg
     1501 aattcaaagg cctacgtcga cgagctcact agtcgcggcc gctttcgaat ctagagcctg
     1561 cagtctcgac aagcttgtcg agaagtacta gaggatgtac aacgcgtgcg cgctgatccg
     1621 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
     1681 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa acctcaggca
     1741 tttgagaagc acacggtcac actgcttccg gtagtcaata aaccggtaaa ccagcaatag
     1801 acataagcgg ctatttaacg accctgccct gaaccgacga ccgggtcatc gtggccggat
     1861 cttgcggccc ctcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc
     1921 tcgcctttca cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct
     1981 tcttcttgtc caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc
     2041 aggcgctcca ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg
     2101 ctgtaccaaa tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc
     2161 ggcgagttcc atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc
     2221 ggatcaaaga gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt
     2281 gtcagcaaga tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg
     2341 tcattgcgct gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg
     2401 atgtcgtcgt gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg
     2461 gaagccgaag tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt
     2521 acggtcaccg taaccagcaa atcaatatca ctgtgtggct
//
