LOCUS       sequence-31033-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-RCKW
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        41..59
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..129
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             145..174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    193..217
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtgaaccgt cagatccgct agcgattacg ccaagctcga aattaaccct cactaaaggg
       61 aacaaaagct ggagctccac cgcggtggcg gccgccatgg agcagaaact catctctgaa
      121 gaggatctga gcccgggcgg atccgagcag aaactcatct ctgaagagga tctgctgcag
      181 gaattcgata tcacaagttt gtacaaaaaa gcaggctccg aattcgccct tatgaaggct
      241 gtgccttata accgaatgaa acttatgatt gtgggaaata ctgggagtgg taaaaccacc
      301 ttattgcagc aattaatgaa aaccaagaaa tcagatcttg gaatgcaaag tgccacagtt
      361 ggcatagatg tgaaagactg gcctatccaa ataagagaca aaagaaagag agatctcgtc
      421 ctaaatgtgt gggattttgc aggtcgtgag gaattctata gtactcatcc ccattttatg
      481 acgcagcgag cattgtacct tgctgtctat gacctcagca agggacaggc tgaagttgat
      541 gccatgaagc cttggctctt caatataaag gctcgcgctt cttcttcccc tgtgattctc
      601 gttggcacac atttggatgt ttctgatgag aagcaacgca aagcctgcat gagtaaaatc
      661 accaaggaac tcctgaataa gcgagggttc cctgccatac gagattacca ctttgtgaat
      721 gccaccgagg aatctgatgc tttggcaaaa cttcggaaaa ccatcataaa cgagagcctt
      781 aatttcaaga tccgagatca gcttgttgtt ggacagctga ttccagactg ctatgtagaa
      841 cttgaaaaaa tcattttatc ggagcgtaaa aatgtgccaa ttgaatt
//
