LOCUS       sequence-310330-        2683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  N-term Myc 3C cleavable Spec resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 33 pCOLI_S_Myc
FEATURES             Location/Qualifiers
     source          1..2683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(130..221)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(269..1007)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     1217..1236
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1217..1235
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1273..1295
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1315..1332
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1345..1374
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1381..1410
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1417..1446
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1453..1482
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1489..1518
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1525..1554
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1573..1596
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(1789..1807)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1803..1850
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(2021..2683,1..129))
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 cttcaggccg ccatccactg cggagccgta caaatgtacg gccagcaacg tcggttcgag
       61 atggcgctcg atgacgccaa ctacctctga tagttgagtc gatacttcgg cgatcaccgc
      121 ttccctcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
      181 gagcggatac atatttgaat gtatttagaa aaataaacaa atagctagct cactcggtcg
      241 ctacgctccg ggcgtgagac tgcggcgggc gctgcggaca catacaaagt tacccacaga
      301 ttccgtggat aagcagggga ctaacatgtg aggcaaaaca gcagggccgc gccggtggcg
      361 tttttccata ggctccgccc tcctgccaga gttcacataa acagacgctt ttccggtgca
      421 tctgtgggag ccgtgaggct caaccatgaa tctgacagta cgggcgaaac ccgacaggac
      481 ttaaagatcc ccaccgtttc cggcgggtcg ctccctcttg cgctctcctg ttccgaccct
      541 gccgtttacc ggatacctgt tccgcctttc tcccttacgg gaagtgtggc gctttctcat
      601 agctcacaca ctggtatctc ggctcggtgt aggtcgttcg ctccaagctg ggctgtaagc
      661 aagaactccc cgttcagccc gactgctgcg ccttatccgg taactgttca cttgagtcca
      721 acccggaaaa gcacggtaaa acgccactgg cagcagccat tggtaactgg gagttcgcag
      781 aggatttgtt tagctaaaca cgcggttgct cttgaagtgt gcgccaaagt ccggctacac
      841 tggaaggaca gatttggttg ctgtgctctg cgaaagccag ttaccacggt taagcagttc
      901 cccaactgac ttaaccttcg atcaaaccac ctccccaggt ggttttttcg tttacagggc
      961 aaaagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctactgaac
     1021 cgctctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc agccccatac
     1081 gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg agctgactgg
     1141 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
     1201 attaattgcg ttgcgctaat acgactcact atagggagac cacaacggtt tccctccggc
     1261 tccggaaata attttgttta actttaagaa ggagatatac atatgggaag ttctcatcac
     1321 caccatcatc atagttcggg ctccgagcag aaacttatct ctgaggaaga cttaaatggg
     1381 gagcagaagt tgatctcgga ggaagatctg aatggtgagc aaaagttaat ctccgaagaa
     1441 gacttgaatg gcgaacaaaa actgatctca gaggaagatt tgaacggcga acagaagtta
     1501 atctctgagg aggatctgaa cggagaacag aaacttattt cggaagaaga tttgaacggg
     1561 tcgagtggta gtctggaagt tctgttccag gggcccggat cccggtccga catatgagcg
     1621 cgcggaattc aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga
     1681 gcctgcagtc tcgacaagct tgtcgagaag tactagagga tgtacaacgc gtgcgcgctg
     1741 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat
     1801 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaacctc
     1861 aggcatttga gaagcacacg gtcacactgc ttccggtagt caataaaccg gtaaaccagc
     1921 aatagacata agcggctatt taacgaccct gccctgaacc gacgaccggg tcatcgtggc
     1981 cggatcttgc ggcccctcgg cttgaacgaa ttgttagaca ttatttgccg actaccttgg
     2041 tgatctcgcc tttcacgtag tggacaaatt cttccaactg atctgcgcgc gaggccaagc
     2101 gatcttcttc ttgtccaaga taagcctgtc tagcttcaag tatgacgggc tgatactggg
     2161 ccggcaggcg ctccattgcc cagtcggcag cgacatcctt cggcgcgatt ttgccggtta
     2221 ctgcgctgta ccaaatgcgg gacaacgtaa gcactacatt tcgctcatcg ccagcccagt
     2281 cgggcggcga gttccatagc gttaaggttt catttagcgc ctcaaataga tcctgttcag
     2341 gaaccggatc aaagagttcc tccgccgctg gacctaccaa ggcaacgcta tgttctcttg
     2401 cttttgtcag caagatagcc agatcaatgt cgatcgtggc tggctcgaag atacctgcaa
     2461 gaatgtcatt gcgctgccat tctccaaatt gcagttcgcg cttagctgga taacgccacg
     2521 gaatgatgtc gtcgtgcaca acaatggtga cttctacagc gcggagaatc tcgctctctc
     2581 caggggaagc cgaagtttcc aaaaggtcgt tgatcaaagc tcgccgcgtt gtttcatcaa
     2641 gccttacggt caccgtaacc agcaaatcaa tatcactgtg tgg
//
