LOCUS       sequence-310339-        8922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NLS-V5-MCP-APEX2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8922)
  AUTHORS   Han S, Zhao BS, Myers SA, Carr SA, He C, Ting AY
  TITLE     RNA-protein interaction mapping via MS2- or Cas13-based APEX 
            targeting.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117.
  PUBMED    32839320
REFERENCE   2  (bases 1 to 8922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117."
COMMENT     Sequence Label: MCP-APEX2
FEATURES             Location/Qualifiers
     source          1..8922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1710..2210
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2129..2147
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2222..2617
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2677..2794
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2911..3214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3215..3418
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3369..3389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3441..3465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3489..3509
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3516..3557
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    4775..5363
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4828..4848)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5246..5257)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5435..5668
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5746..5867
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5783..5802)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5837..5856
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5907..6042
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6062..6081)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6063..6081)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6091..6108)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6091..6107)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6100..6122)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6249..6704
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6336..6355)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6546..6567
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6730..6834
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6835..6903,6904..7695)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7053..7072)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7866..8454
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8355..8374
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8608..8625
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8742..8763
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8778..8783,8784..8801,8802..8808)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8816..8832
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8821..8843
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8840..8856
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8840..8856
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8875..8895
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8877..8895
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     1741 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     1801 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     1861 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     1921 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     1981 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2041 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2101 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     2161 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     2221 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     2281 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     2341 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     2401 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     2461 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     2521 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     2581 tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt
     2641 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2701 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2761 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2821 tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc
     2881 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2941 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3001 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3061 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3121 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3181 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3241 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3301 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3361 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     3421 ctggctaact gtcgggatca acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa
     3481 ccatggctcc aaagaagaag cgtaaggtag gcagcggcaa gcccatcccc aaccccctgc
     3541 tgggcctgga cagcaccggc agcgctagca atttcactca atttgtgctc gtggataacg
     3601 gaggaacggg cgacgtgacc gtggcaccaa gcaactttgc taatggagtg gccgagtgga
     3661 ttagctcaaa tagcaggtcc caggcttaca aggtcacctg tagtgtccgg caatcatccg
     3721 cccagaatcg gaaatatact attaaggtag aagtgccgaa agtggcaact caaaccgtgg
     3781 ggggcgagga attgcctgtg gccggctgga gaagctacct caacatggaa ctcaccattc
     3841 caatcttcgc cacaaacagc gactgtgagc tcatcgtcaa agccatgcag ggtctgctga
     3901 aggacggcaa tcctattcca tctgcaatcg cggccaattc cggcatttat ggatccaagg
     3961 gctcgggctc gacctcgggc tccggaggga aatcataccc aacagtgtcc gcagactacc
     4021 aggatgccgt ggagaaagcc aagaagaagc tgagagggtt tatcgcagaa aagaggtgcg
     4081 cacctctgat gctgagactg gctttccaca gcgcaggcac ctttgacaag ggaaccaaaa
     4141 caggcggacc ctttggaaca atcaagcacc ctgctgaact ggcacatagt gccaacaatg
     4201 ggctggacat cgccgtgcgg ctgctggaac ctctgaaagc agagttccca attctgtcct
     4261 acgccgattt ttatcagctg gcaggagtgg tcgctgtgga ggtcactggg ggccccaagg
     4321 tgcctttcca cccaggacgg gaggacaaac cagaaccacc tccagagggg cgcctgccag
     4381 atccgacaaa gggctccgac catctgcgag atgtgtttgg gaaagctatg ggcctgactg
     4441 accaggatat cgtcgcactg tctggagggc acaccattgg cgccgctcat aaggaaaggt
     4501 caggcttcga gggaccctgg acaagcaacc ccctgatttt cgacaattct tactttactg
     4561 aactgctgag tggagagaag gaagggctgc tgcagctgcc cagcgacaaa gccctgctgt
     4621 ccgatcccgt gttcagacct ctggtcgata agtatgcagc cgacgaggat gcttttttcg
     4681 cagattacgc agaagcacat cagaagctgt cagaactggg atttgccgac gcctaatgat
     4741 aagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt acaaaatttg
     4801 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     4861 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     4921 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     4981 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     5041 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     5101 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     5161 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     5221 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     5281 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     5341 ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta
     5401 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5461 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5521 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5581 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5641 ttttagtcag tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt
     5701 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca
     5761 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     5821 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc
     5881 tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     5941 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     6001 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc
     6061 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
     6121 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     6181 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     6241 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     6301 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     6361 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     6421 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     6481 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     6541 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     6601 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     6661 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     6721 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6781 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     6841 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     6901 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     6961 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     7021 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     7081 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     7141 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     7201 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     7261 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     7321 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     7381 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     7441 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     7501 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     7561 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     7621 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     7681 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     7741 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     7801 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     7861 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     7921 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     7981 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     8041 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     8101 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     8161 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     8221 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     8281 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     8341 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     8401 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     8461 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     8521 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     8581 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     8641 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     8701 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     8761 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     8821 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt
     8881 aaccctcact aaagggaaca aaagctggag ctgcaagctt aa
//
