LOCUS       sequence-310341-       10971 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BPNLS-dRfxCas13d-dsRBD-BPNLS-HA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10971)
  AUTHORS   Han S, Zhao BS, Myers SA, Carr SA, He C, Ting AY
  TITLE     RNA-protein interaction mapping via MS2- or Cas13-based APEX 
            targeting.
  JOURNAL   Proc Natl Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117.
  PUBMED    32839320
REFERENCE   2  (bases 1 to 10971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2020 Aug 24. pii: 2006617117. doi: 
            10.1073/pnas.2006617117."
COMMENT     Sequence Label: dCas13d-dsRBD
FEATURES             Location/Qualifiers
     source          1..10971
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..227
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             228..408
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    455..580
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1073..1306
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1491..1535
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1710..2210
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     2129..2147
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             2222..2617
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    2677..2794
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2911..3214
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3215..3418
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3369..3389
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    3441..3465
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3519..3539
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3549..6446
                     /codon_start=1
                     /product="RNA-targeting Type VI CRISPR protein from 
                     Ruminococcus flavefaciens strain XPD3002 (Konermann et al.,
                     2018)"
                     /label=RfxCas13d
                     /translation="IEKKKSFAKGMGVKSTLVSGSKVYMTTFAEGSDARLEKIVEGDSI
                     RSVNEGEAFSAEMADKNAGYKIGNAKFSHPKGYAVVANNPLYTGPVQQDMLGLKETLEK
                     RYFGESADGNDNICIQVIHNILDIEKILAEYITNAAYAVNNISGLDKDIIGFGKFSTVY
                     TYDEFKDPEHHRAAFNNNDKLINAIKAQYDEFDNFLDNPRLGYFGQAFFSKEGRNYIIN
                     YGNECYDILALLSGLAHWVVANNEEESRISRTWLYNLDKNLDNEYISTLNYLYDRITNE
                     LTNSFSKNSAANVNYIAETLGINPAEFAEQYFRFSIMKEQKNLGFNITKLREVMLDRKD
                     MSEIRKNHKVFDSIRTKVYTMMDFVIYRYYIEEDAKVAAANKSLPDNEKSLSEKDIFVI
                     NLRGSFNDDQKDALYYDEANRIWRKLENIMHNIKEFRGNKTREYKKKDAPRLPRILPAG
                     RDVSAFSKLMYALTMFLDGKEINDLLTTLINKFDNIQSFLKVMPLIGVNAKFVEEYAFF
                     KDSAKIADELRLIKSFARMGEPIADARRAMYIDAIRILGTNLSYDELKALADTFSLDEN
                     GNKLKKGKHGMRNFIINNVISNKRFHYLIRYGDPAHLHEIAKNEAVVKFVLGRIADIQK
                     KQGQNGKNQIDRYYETCIGKDKGKSVSEKVDALTKIITGMNYDQFDKKRSVIEDTGREN
                     AEREKFKKIISLYLTVIYHILKNIVNINARYVIGFHCVERDAQLYKEKGYDINLKKLEE
                     KGFSSVTKLCAGIDETAPDKRKDVEKEMAERAKESIDSLESANPKLYANYIKYSDEKKA
                     EEFTRQINREKAKTALNAYLRNTKWNVIIREDLLRIDNKTCTLFANKAVALEVARYVHA
                     YINDIAEVNSYFQLYHYIMQRIIMNERYEKSSGKVSEYFDAVNDEKKYNDRLLKLLCVP
                     FGYCIPRFKNLSIEALFDRNEAAKFDKEKKKVSGNS"
     CDS             6735..6755
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6756..6782
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    6824..7412
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6877..6897)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7295..7306)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7484..7717
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7795..7916
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7832..7851)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7886..7905
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7956..8091
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8111..8130)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8112..8130)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8140..8157)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8140..8156)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8149..8171)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8298..8753
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8385..8404)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8595..8616
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8779..8883
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8884..8952,8953..9744)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9102..9121)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9915..10503
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10404..10423
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10657..10674
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10791..10812
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10827..10832,10833..10850,10851..10857)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10865..10881
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10870..10892
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10889..10905
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10889..10905
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10924..10944
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10926..10944
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
       61 ccttacaagg agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc
      121 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
      181 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
      241 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
      301 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
      361 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
      421 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
      481 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
      541 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
      601 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
      661 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
      721 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
      781 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
      841 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
      901 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
      961 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     1021 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     1081 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     1141 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     1201 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     1261 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     1321 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     1381 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     1441 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     1501 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     1561 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     1621 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     1681 agacccacct cccaaccccg aggggaccct tgcgcctttt ccaaggcagc cctgggtttg
     1741 cgcagggacg cggctgctct gggcgtggtt ccgggaaacg cagcggcgcc gaccctgggt
     1801 ctcgcacatt cttcacgtcc gttcgcagcg tcacccggat cttcgccgct acccttgtgg
     1861 gccccccggc gacgcttcct gctccgcccc taagtcggga aggttccttg cggttcgcgg
     1921 cgtgccggac gtgacaaacg gaagccgcac gtctcactag taccctcgca gacggacagc
     1981 gccagggagc aatggcagcg cgccgaccgc gatgggctgt ggccaatagc ggctgctcag
     2041 cagggcgcgc cgagagcagc ggccgggaag gggcggtgcg ggaggcgggg tgtggggcgg
     2101 tagtgtgggc cctgttcctg cccgcgcggt gttccgcatt ctgcaagcct ccggagcgca
     2161 cgtcggcagt cggctccctc gttgaccgaa tcaccgacct ctctccccag ggggtaccac
     2221 catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa cggctacaat
     2281 caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct ctagcgacgg
     2341 ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg cagaactcgt
     2401 ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg tcgcgatcgg
     2461 aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc ttctcgatct
     2521 gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg cagttgggat
     2581 tcgtgaattg ctgccctctg gttatgtgtg ggagggcctg cagctgcagt agtaagaatt
     2641 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     2701 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     2761 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     2821 tccactttgg cgccggctcg agggggcccg gggttattaa tagtaatcaa ttacggggtc
     2881 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     2941 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     3001 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     3061 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     3121 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     3181 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     3241 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     3301 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     3361 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
     3421 ctggctaact gtcgggatca acaagtttgt acaaaaaagc aggcttcgaa ggagatagaa
     3481 ccatgaagcg gactgctgat ggcagtgaat ttgagagccc caagaagaag agaaaggtgg
     3541 aggccagcat cgaaaaaaaa aagtccttcg ccaagggcat gggcgtgaag tccacactcg
     3601 tgtccggctc caaagtgtac atgacaacct tcgccgaagg cagcgacgcc aggctggaaa
     3661 agatcgtgga gggcgacagc atcaggagcg tgaatgaggg cgaggccttc agcgctgaaa
     3721 tggccgataa aaacgccggc tataagatcg gcaacgccaa attcagccat cctaagggct
     3781 acgccgtggt ggctaacaac cctctgtata caggacccgt ccagcaggat atgctcggcc
     3841 tgaaggaaac tctggaaaag aggtacttcg gcgagagcgc tgatggcaat gacaatattt
     3901 gtatccaggt gatccataac atcctggaca ttgaaaaaat cctcgccgaa tacattacca
     3961 acgccgccta cgccgtcaac aatatctccg gcctggataa ggacattatt ggattcggca
     4021 agttctccac agtgtatacc tacgacgaat tcaaagaccc cgagcaccat agggccgctt
     4081 tcaacaataa cgataagctc atcaacgcca tcaaggccca gtatgacgag ttcgacaact
     4141 tcctcgataa ccccagactc ggctatttcg gccaggcctt tttcagcaag gagggcagaa
     4201 attacatcat caattacggc aacgaatgct atgacattct ggccctcctg agcggactgg
     4261 cgcactgggt ggtcgctaac aacgaagaag agtccaggat ctccaggacc tggctctaca
     4321 acctcgataa gaacctcgac aacgaataca tctccaccct caactacctc tacgacagga
     4381 tcaccaatga gctgaccaac tccttctcca agaactccgc cgccaacgtg aactatattg
     4441 ccgaaactct gggaatcaac cctgccgaat tcgccgaaca atatttcaga ttcagcatta
     4501 tgaaagagca gaaaaacctc ggattcaata tcaccaagct cagggaagtg atgctggaca
     4561 ggaaggatat gtccgagatc aggaaaaatc ataaggtgtt cgactccatc aggaccaagg
     4621 tctacaccat gatggacttt gtgatttata ggtattacat cgaagaggat gccaaggtgg
     4681 ctgccgccaa taagtccctc cccgataatg agaagtccct gagcgagaag gatatctttg
     4741 tgattaacct gaggggctcc ttcaacgacg accagaagga tgccctctac tacgatgaag
     4801 ctaatagaat ttggagaaag ctcgaaaata tcatgcacaa catcaaggaa tttaggggaa
     4861 acaagacaag agagtataag aagaaggacg cccctagact gcccagaatc ctgcccgctg
     4921 gccgtgatgt ttccgccttc agcaaactca tgtatgccct gaccatgttc ctggatggca
     4981 aggagatcaa cgacctcctg accaccctga ttaataaatt cgataacatc cagagcttcc
     5041 tgaaggtgat gcctctcatc ggagtcaacg ctaagttcgt ggaggaatac gcctttttca
     5101 aagactccgc caagatcgcc gatgagctga ggctgatcaa gtccttcgct agaatgggag
     5161 aacctattgc cgatgccagg agggccatgt atatcgacgc catccgtatt ttaggaacca
     5221 acctgtccta tgatgagctc aaggccctcg ccgacacctt ttccctggac gagaacggaa
     5281 acaagctcaa gaaaggcaag cacggcatga gaaatttcat tattaataac gtgatcagca
     5341 ataaaaggtt ccactacctg atcagatacg gtgatcctgc ccacctccat gagatcgcca
     5401 aaaacgaggc cgtggtgaag ttcgtgctcg gcaggatcgc tgacatccag aaaaaacagg
     5461 gccagaacgg caagaaccag atcgacaggt actacgaaac ttgtatcgga aaggataagg
     5521 gcaagagcgt gagcgaaaag gtggacgctc tcacaaagat catcaccgga atgaactacg
     5581 accaattcga caagaaaagg agcgtcattg aggacaccgg cagggaaaac gccgagaggg
     5641 agaagtttaa aaagatcatc agcctgtacc tcaccgtgat ctaccacatc ctcaagaata
     5701 ttgtcaatat caacgccagg tacgtcatcg gattccattg cgtcgagcgt gatgctcaac
     5761 tgtacaagga gaaaggctac gacatcaatc tcaagaaact ggaagagaag ggattcagct
     5821 ccgtcaccaa gctctgcgct ggcattgatg aaactgcccc cgataagaga aaggacgtgg
     5881 aaaaggagat ggctgaaaga gccaaggaga gcattgacag cctcgagagc gccaacccca
     5941 agctgtatgc caattacatc aaatacagcg acgagaagaa agccgaggag ttcaccaggc
     6001 agattaacag ggagaaggcc aaaaccgccc tgaacgccta cctgaggaac accaagtgga
     6061 atgtgatcat cagggaggac ctcctgagaa ttgacaacaa gacatgtacc ctgttcgcaa
     6121 acaaggccgt cgccctggaa gtggccaggt atgtccacgc ctatatcaac gacattgccg
     6181 aggtcaattc ctacttccaa ctgtaccatt acatcatgca gagaattatc atgaatgaga
     6241 ggtacgagaa aagcagcgga aaggtgtccg agtacttcga cgctgtgaat gacgagaaga
     6301 agtacaacga taggctcctg aaactgctgt gtgtgccttt cggctactgt atccccaggt
     6361 ttaagaacct gagcatcgag gccctgttcg ataggaacga ggccgccaag ttcgacaagg
     6421 agaaaaagaa ggtgtccggc aattccggat ccggagcagg cgatttgtcc gcaggtttct
     6481 tcatggaaga actgaacact tatcgccaga aacagggagt tgtgctgaag taccaagagt
     6541 tgccgaactc aggcccacct cacgaccgaa gattcacttt tcaagtaatt atcgatggta
     6601 gagagttccc agaaggcgaa ggccgaagta agaaagaagc gaagaacgca gccgctaaat
     6661 tggcagtcga aatcctcaat aaagaaaaga aagcgggatc caagcgcacc gccgatggtt
     6721 ccgagttcga aagccccaag aaaaagcgca aggtctaccc atacgatgtt ccagattacg
     6781 cttaatgata agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta
     6841 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     6901 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     6961 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     7021 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     7081 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     7141 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     7201 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     7261 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     7321 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     7381 gagtcggatc tccctttggg ccgcctcccc gcgtcgactt taagaccaat gacttacaag
     7441 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7501 tcccaacgaa gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7561 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7621 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7681 ctcagaccct tttagtcagt gtggaaaatc tctagcagta cgtatagtag ttcatgtcat
     7741 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7801 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7861 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7921 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     7981 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8041 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     8101 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     8161 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8221 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     8281 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     8341 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     8401 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8461 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8521 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8581 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8641 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     8701 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     8761 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8821 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8881 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8941 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     9001 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     9061 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     9121 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     9181 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     9241 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9301 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9361 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9421 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9481 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9541 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9601 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9661 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9721 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9781 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9841 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9901 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     9961 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
    10021 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
    10081 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
    10141 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
    10201 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
    10261 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
    10321 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
    10381 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
    10441 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
    10501 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
    10561 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
    10621 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
    10681 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
    10741 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
    10801 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
    10861 ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag
    10921 cgcgcaatta accctcacta aagggaacaa aagctggagc tgcaagctta a
//
