LOCUS       sequence-310344-        2315 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged Spec resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2315)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2315)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 25 pCOLI_S_NoTag
FEATURES             Location/Qualifiers
     source          1..2315
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     707..726
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        707..725
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             763..785
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(911..929)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      925..972
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(1143..1934)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(1935..2026)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(join(2074..2315,1..497))
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
ORIGIN
        1 ctccctcttg cgctctcctg ttccgaccct gccgtttacc ggatacctgt tccgcctttc
       61 tcccttacgg gaagtgtggc gctttctcat agctcacaca ctggtatctc ggctcggtgt
      121 aggtcgttcg ctccaagctg ggctgtaagc aagaactccc cgttcagccc gactgctgcg
      181 ccttatccgg taactgttca cttgagtcca acccggaaaa gcacggtaaa acgccactgg
      241 cagcagccat tggtaactgg gagttcgcag aggatttgtt tagctaaaca cgcggttgct
      301 cttgaagtgt gcgccaaagt ccggctacac tggaaggaca gatttggttg ctgtgctctg
      361 cgaaagccag ttaccacggt taagcagttc cccaactgac ttaaccttcg atcaaaccac
      421 ctccccaggt ggttttttcg tttacagggc aaaagattac gcgcagaaaa aaaggatctc
      481 aagaagatcc tttgatcttt tctactgaac cgctctagat ttcagtgcaa tttatctctt
      541 caaatgtagc acctgaagtc agccccatac gatataagtt gtaattctca tgttagtcat
      601 gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgaga
      661 tcccggtgcc taatgagtga gctaacttac attaattgcg ttgcgctaat acgactcact
      721 atagggagac cacaacggtt tccctccggc tccggaaata attttgttta actttaagaa
      781 ggagactgga tccgaatcta gagcctgcag tctcgacaag cttgtcgaga agtactagag
      841 gatgtacaac gcgtgcgcgc tgatccggct gctaacaaag cccgaaagga agctgagttg
      901 gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
      961 aggggttttt tgctgaaacc tcaggcattt gagaagcaca cggtcacact gcttccggta
     1021 gtcaataaac cggtaaacca gcaatagaca taagcggcta tttaacgacc ctgccctgaa
     1081 ccgacgaccg ggtcatcgtg gccggatctt gcggcccctc ggcttgaacg aattgttaga
     1141 cattatttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     1201 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     1261 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     1321 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     1381 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     1441 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     1501 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     1561 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     1621 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     1681 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     1741 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     1801 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     1861 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     1921 accgcttccc tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     1981 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagc tagctcactc
     2041 ggtcgctacg ctccgggcgt gagactgcgg cgggcgctgc ggacacatac aaagttaccc
     2101 acagattccg tggataagca ggggactaac atgtgaggca aaacagcagg gccgcgccgg
     2161 tggcgttttt ccataggctc cgccctcctg ccagagttca cataaacaga cgcttttccg
     2221 gtgcatctgt gggagccgtg aggctcaacc atgaatctga cagtacgggc gaaacccgac
     2281 aggacttaaa gatccccacc gtttccggcg ggtcg
//
