LOCUS       sequence-310348-        2363 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Untagged Kan resistant bacterial expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Altmannova V, Blaha A, Astrinidis S, Reichle H, Weir JR
  TITLE     InteBac - An integrated bacterial and baculovirus expression vector 
            suite.
  JOURNAL   Protein Sci. 2020 Sep 21. doi: 10.1002/pro.3957.
  PUBMED    32955754
REFERENCE   2  (bases 1 to 2363)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Sep 21. doi: 10.1002/pro.3957."
COMMENT     Sequence Label: 41 pCOLI_K_NoTag
FEATURES             Location/Qualifiers
     source          1..2363
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(27..118)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(134..883)
                     /direction=LEFT
                     /label=RSF ori
                     /note="Plasmids containing the RSF 1030 origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     primer_bind     complement(144..161)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(384..403)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1079..1098
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1079..1097
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             1135..1157
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1283..1301)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1297..1344
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(join(1574..2363,1..26))
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(join(1574..2363,1..26))
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2297..2316
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gacgtttccc gttgaatatg gctcatactc ttcctttttc aatattattg aagcatttat
       61 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
      121 ggcatgcagc gctcttccgc ttcctcgctc actgactcgc tacgctcggt cgttcgactg
      181 cggcgagcgg tgtcagctca ctcaaaagcg gtaatacggt tatccacaga atcaggggat
      241 aaagccggaa agaacatgtg agcaaaaagc aaagcaccgg aagaagccaa cgccgcaggc
      301 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagccagag
      361 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
      421 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
      481 aagcgtggcg ctttctcata gctcacgctg ttggtatctc agttcggtgt aggtcgttcg
      541 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
      601 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccat
      661 tggtaactga tttagaggac tttgtcttga agttatgcac ctgttaaggc taaactgaaa
      721 gaacagattt tggtgagtgc ggtcctccaa cccacttacc ttggttcaaa gagttggtag
      781 ctcagcgaac cttgagaaaa ccaccgttgg tagcggtggt ttttctttat ttatgagatg
      841 atgaatcaat cggtctatca agtcaacgaa cagctattcc gttactctag atttcagtgc
      901 aatttatctc ttcaaatgta gcacctgaag tcagccccat acgatataag ttgtaattct
      961 catgttagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg
     1021 catcggtcga gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgcta
     1081 atacgactca ctatagggag accacaacgg tttccctccg gctccggaaa taattttgtt
     1141 taactttaag aaggagactg gatccgaatc tagagcctgc agtctcgaca agcttgtcga
     1201 gaagtactag aggatgtaca acgcgtgcgc gctgatccgg ctgctaacaa agcccgaaag
     1261 gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct
     1321 aaacgggtct tgaggggttt tttgctgaaa cctcaggcat ttgagaagca cacggtcaca
     1381 ctgcttccgg tagtcaataa accggtaaac cagcaataga cataagcggc tatttaacga
     1441 ccctgccctg aaccgacgac aagctgacga ccgggtctcc gcaagtggca cttttcgggg
     1501 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     1561 catgaattaa ttcttagaaa aactcatcga gcatcaaatg aaactgcaat ttattcatat
     1621 caggattatc aataccatat ttttgaaaaa gccgtttctg taatgaagga gaaaactcac
     1681 cgaggcagtt ccataggatg gcaagatcct ggtatcggtc tgcgattccg actcgtccaa
     1741 catcaataca acctattaat ttcccctcgt caaaaataag gttatcaagt gagaaatcac
     1801 catgagtgac gactgaatcc ggtgagaatg gcaaaagttt atgcatttct ttccagactt
     1861 gttcaacagg ccagccatta cgctcgtcat caaaatcact cgcatcaacc aaaccgttat
     1921 tcattcgtga ttgcgcctga gcgagacgaa atacgcggtc gctgttaaaa ggacaattac
     1981 aaacaggaat cgaatgcaac cggcgcagga acactgccag cgcatcaaca atattttcac
     2041 ctgaatcagg atattcttct aatacctgga atgctgtttt cccggggatc gcagtggtga
     2101 gtaaccatgc atcatcagga gtacggataa aatgcttgat ggtcggaaga ggcataaatt
     2161 ccgtcagcca gtttagtctg accatctcat ctgtaacatc attggcaacg ctacctttgc
     2221 catgtttcag aaacaactct ggcgcatcgg gcttcccata caatcgatag attgtcgcac
     2281 ctgattgccc gacattatcg cgagcccatt tatacccata taaatcagca tccatgttgg
     2341 aatttaatcg cggcctagag caa
//
