LOCUS       sequence-31035-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     The Parkinson Disease-associated Leucine-rich Repeat Kinase 2 
            (LRRK2) Is a Dimer That Undergoes Intramolecular Autophosphorylation
  JOURNAL   J Biol Chem. 2008 Jun 13;283(24):16906-14.
  PUBMED    18397888
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2008-06-13"; volume: "283(24)"; pages: "16906-14"
COMMENT     Sequence Label: 2XMyc-LRRK2-RCK
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        41..59
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     regulatory      91..100
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             100..129
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             145..174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    193..217
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agtgaaccgt cagatccgct agcgattacg ccaagctcga aattaaccct cactaaaggg
       61 aacaaaagct ggagctccac cgcggtggcg gccgccatgg agcagaaact catctctgaa
      121 gaggatctga gcccgggcgg atccgagcag aaactcatct ctgaagagga tctgctgcag
      181 gaattcgata tcacaagttt gtacaaaaaa gcaggctccg aattcgccct tgctgtgcct
      241 tataaccgaa tgaaacttat gattgtggga aatactggga gtggtaaaac caccttattg
      301 cagcaattaa tgaaaaccaa gaaatcagat cttggaatgc aaagtgccac agttggcata
      361 gatgtgaaag actggcctat ccaaataaga gacaaaagaa agagagatct cgtcctaaat
      421 gtgtgggatt ttgcaggtcg tgaggaattc tatagtactc atccccattt tatgacgcag
      481 cgagcattgt accttgctgt ctatgacctc agcaagggac aggctgaagt tgatgccatg
      541 aagccttggc tcttcaatat aaaggctcgc gcttcttctt cccctgtgat tctcgttggc
      601 acacatttgg atgtttctga tgagaagcaa cgcaaagcct gcatgagtaa aatcaccaag
      661 gaactcctga ataagcgagg gttccctgcc atacgagatt accactttgt gaatgccacc
      721 gaggaatctg atgctttggc aaaacttcgg aaaaccatca taaacgagag ccttaatttc
      781 aagatccgag atcagcttgt tgttggacag ctgattccag actgctatgt agaacttgaa
      841 aaatcatttt atcggagcgt aaaaatgtgc caatt
//
