LOCUS       sequence-310361-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows for constituitive mammalian expression of the SARS-CoV-2 3CL 
            protease with C145A mutation via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-SARS-CoV-2-3CL-C145A
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagtggtt ttagaaaaat ggcattccca tctggtaaag ttgagggttg tatggtacaa
       61 gtaacttgtg gtacaactac acttaacggt ctttggcttg atgacgtagt ttactgtcca
      121 agacatgtga tctgcacctc tgaagacatg cttaacccta attatgaaga tttactcatt
      181 cgtaagtcta atcataattt cttggtacag gctggtaatg ttcaactcag ggttattgga
      241 cattctatgc aaaattgtgt acttaagctt aaggttgata cagccaatcc taagacacct
      301 aagtataagt ttgttcgcat tcaaccagga cagacttttt cagtgttagc ttgttacaat
      361 ggttcaccat ctggtgttta ccaatgtgct atgaggccca atttcactat taagggttca
      421 ttccttaatg gttcagccgg tagtgttggt tttaacatag attatgactg tgtctctttt
      481 tgttacatgc accatatgga attaccaact ggagttcatg ctggcacaga cttagaaggt
      541 aacttttatg gaccttttgt tgacaggcaa acagcacaag cagctggtac ggacacaact
      601 attacagtta atgttttagc ttggttgtac gctgctgtta taaatggaga caggtggttt
      661 ctcaatcgat ttaccacaac tcttaatgac tttaaccttg tggctatgaa gtacaattat
      721 gaacctctaa cacaagacca tgttgacata ctaggacctc tttctgctca aactggaatt
      781 gccgttttag atatgtgtgc ttcattaaaa gaattactgc aaaatggtat gaatggacgt
      841 accatattgg gtagtgcttt attagaagat gaatttacac cttttgatgt tgttagacaa
      901 tgctcaggtg ttactttcca ataa
//
