LOCUS       sequence-310363-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Allows for constituitive mammalian expression of the SARS-CoV 3CL 
            protease with C145A mutation via transfection or lentivirus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Resnick SJ, Iketani S, Hong SJ, Zask A, Liu H, Kim S, Melore S, Lin 
            FY, Nair MS, Huang Y, Lee S, Tay NES, Rovis T, Yang HW, Xing L, 
            Stockwell BR, Ho DD, Chavez A
  TITLE     Inhibitors of Coronavirus 3CL Proteases Protect Cells from 
            Protease-Mediated Cytotoxicity.
  JOURNAL   J Virol. 2021 Jun 24;95(14):e0237420. doi: 10.1128/JVI.02374-20. 
            Epub 2021 Jun 24.
  PUBMED    33910954
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol."; 
            date: "2021-06-24"; volume: "95(14):e0237420. doi"; pages: " 
            10.1128/JVI.02374-20. Epub 2021 Jun 24"
COMMENT     Sequence Label: pLEX307-SARS-CoV-3CL-C145A
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgagtggtt ttaggaaaat ggcattcccg tcaggcaaag ttgaagggtg catggtacaa
       61 gtaacctgtg gaactacaac tcttaatgga ttgtggttgg atgacacagt atactgtcca
      121 agacatgtca tttgcacagc agaagacatg cttaatccta actatgaaga tctgctcatt
      181 cgcaaatcca accatagctt tcttgttcag gctggcaatg ttcaacttcg tgttattggc
      241 cattctatgc aaaattgtct gcttaggctt aaagttgata cttctaaccc taagacaccc
      301 aagtataaat ttgtccgtat ccaacctggt caaacatttt cagttctagc atgctacaat
      361 ggttcaccat ctggtgttta tcagtgtgcc atgagaccta atcataccat taaaggttct
      421 ttccttaatg gatcagccgg tagtgttggt tttaacattg attatgattg cgtgtctttc
      481 tgctatatgc atcatatgga gcttccaaca ggagtacacg ctggtactga cttagaaggt
      541 aaattctatg gtccatttgt tgacagacaa actgcacagg ctgcaggtac agacacaacc
      601 ataacattaa atgttttggc atggctgtat gctgctgtta tcaatggtga taggtggttt
      661 cttaatagat tcaccactac tttgaatgac tttaaccttg tggcaatgaa gtacaactat
      721 gaacctttga cacaagatca tgttgacata ttgggacctc tttctgctca aacaggaatt
      781 gccgtcttag atatgtgtgc tgctttgaaa gagctgctgc agaatggtat gaatggtcgt
      841 actatccttg gtagcactat tttagaagat gagtttacac catttgatgt tgttagacaa
      901 tgctctggtg ttaccttcca ataa
//
